Method for preparing salvianolic acid A of salvia miltiorrhiza

A technology of salvianolic acid and salvia miltiorrhiza, applied in the field of medicine, can solve the problems of low content, neglect, and inability to carry out industrial production.

Inactive Publication Date: 2011-10-19
PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Salvia miltiorrhiza is a classic traditional Chinese medicine that promotes blood circulation and removes blood stasis. Its chemical components that have the effect of promoting blood circulation and removing blood stasis are mainly water-soluble components. Among the active ingredients, salvianolic acid B has the highest content in the total phenolic acids of salvia miltiorrhiza, therefore, the focus of people's development is on salvianolic acid B, but salvianolic acid A has the best activity in the water-soluble components of salvia miltiorrhiza (Du Guanhua [Basic Medicine and Clinical Medicine] , 2000, 20(5): 10~14], Hu Yiyang [Acta Pharmacology of Traditional Chinese Medicine, 1997, 18(5): 478-480]), but the content of salvianolic acid A in Danshen is very low, because it cannot be industrialized being ignored

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing salvianolic acid A of salvia miltiorrhiza
  • Method for preparing salvianolic acid A of salvia miltiorrhiza
  • Method for preparing salvianolic acid A of salvia miltiorrhiza

Examples

Experimental program
Comparison scheme
Effect test

experiment approach 1

[0022] Experimental scheme 1: Obtain the salvianolic acid A extract according to Example 1 of the patent application number 200610145453.9;

experiment approach 2

[0023] Experimental scheme 2: Obtain the salvianolic acid A extract according to the preparation method of the salvianolic acid A extract of the present invention: Salvia miltiorrhiza is extracted with water and ethanol, the extract is recovered and concentrated with ethanol, the pH value is adjusted to 4.0, and heated at 120°C for 3 hours to obtain the reaction solution. The reaction solution was centrifuged at 25°C, separated by AB-8 macroporous resin column chromatography, washed with water first, discarded the eluent, then eluted with 20% ethanol, discarded the eluent, and then used 40% concentration of Elution with ethanol, collect the eluent, concentrate the eluent to an ethanol concentration of 10%; separate the concentrated solution with a polyamide column, first elute with 40% ethanol solution, discard the eluent, and then use 80% ethanol The solution was eluted, the eluate was collected, the eluate was concentrated with ethanol to the utmost extent, and dried to obtai...

Embodiment 1

[0041] A preparation method of salvianolic acid A extract, extracting salvia miltiorrhiza with water, concentrating the extract, adjusting the pH value to 3.5, heating at 105°C for 6 hours to obtain a reaction liquid, centrifuging the reaction liquid at 20°C, passing through HPD-100 macroporous resin Separation by column chromatography; wash with water first, discard the eluent, then elute with 10% ethanol, discard the eluent, then elute with 30% ethanol, collect the eluent, and concentrate the eluent to ethanol The concentration is 5%; the concentrated solution is separated by polyamide chromatography column, first eluted with 30% ethanol solution, discarded the eluent, then eluted with 70% ethanol solution, collected the eluent, and concentrated ethanol to exhausted, dried to obtain the salvianolic acid A extract.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting salvianolic acid A, which is characterized in that a new process method is adopted to obtain salvianolic acid A extract, the yield of the salvianolic acid A in the salvianolic acid A extract prepared with the method is more than five thousandth, thus saving a great quantity of resources. The invention further discloses a method for preparing the salvianolic acid A, which comprises the steps of adopting a medium pressure liquid phase preparation method, wherein eluent has two phases to carry out isocratic or gradient elution to obtain high-purity salvianolic acid A with related substances, such as salvianolic acid F and salvianolic acid C, with content lower than 0.5 percent. The quality of the salvianolic acid A obtained by using the method is more uniform and more stable.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method of salvianolic acid A. Background technique [0002] Salvia miltiorrhiza is a classic traditional Chinese medicine that promotes blood circulation and removes blood stasis. Its chemical components that have the effect of promoting blood circulation and removing blood stasis are mainly water-soluble components. Among the active ingredients, salvianolic acid B has the highest content in the total phenolic acids of salvia miltiorrhiza, therefore, the focus of people's development is on salvianolic acid B, but salvianolic acid A has the best activity in the water-soluble components of salvia miltiorrhiza (Du Guanhua [Basic Medicine and Clinical Medicine] , 2000, 20(5): 10~14], Hu Yiyang [Acta Pharmacology of Traditional Chinese Medicine, 1997, 18(5): 478-480]), but the content of salvianolic acid A in Danshen is very low, because it cannot be industrialized And...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56
Inventor 顾群渠守峰孙德杰郭小鹏郝华孙学伟
Owner PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap