384 results about "Tanshinone IIA" patented technology

The invention relates to a method for extracting tanshinone which comprises the following two steps: first, alcohol extraction process: separating the extraction liquid and dregs with the danshen liposolubility content from danshen medicine materical crude slices or disintegrated danshen medicinal materials by bath method, hypersonic extraction or microwave extraction in condition of normal temperature or heating, and reserving, adding correct concentration alcohol into the dregs, inverse flow extracting with heating to get the extraction, which combined with the extraction get from the first extraction, removing the solid impurities by the methods of filtration, centrifugation or deposition, and reserving. Second, isolation method separation: adding the medicine liquid get from the said alcohol extraction process to a macroreticular resinous adsorbent column, gradient eluting by alcohol with a finite concentration respectively to get the extraction I (with cryptotanshinone and dihydrotanshinone I) and extraction II (with tanshinone IIA and tanshinone I).

The invention relates to tanshinone compounds in the technical field of medicine and applications thereof. The tanshinone compounds contain tanshinone I, tanshinone IIA, cryptotanshinone and dihydrotanshinone I and the structural formulas are shown as below. The method of the invention uses the tanshinone compounds as the active ingredients of the androgen receptor antagonist; the compounds are used alone or used along with the excipient to prepare oral agents or non-oral agents for curing the diseases related to androgen.

The invention relates to a DanShen fingerprint spectrum quality control method, comprising that (1), adding 1.0g DanShen powder into carbinol to extract via microwave, (2), washing flow phase gradient that the Alltima C18 column is 4.6mm, 250mm, and 5mum, checking wavelength is 280nm, flow speed is 1.0ml/min, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is DanShen element, the eighth peak is rosmarinic acid, the ninth peak is alkannic acid, the tenth peak is phenolic acid B, the eleventh peak is phenolic acid B isomer, the fourteenth peak is cryptotanshinone and the fourteenth peak is tanshinone IIA, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 0.21 of DanShen element, 0.91 of rosmarinic acid, 0.93 of alkannic acid, 1.00 of phenolic acid B, 1.04 of phenolic acid B isomer, 0.92 of cryptotanshinone and 1.00 of tanshinone IIA, (5), the DanShen planting collecting method. The invention can control the quality of materials to assure the stable quality of product.

The invention provides an ultrafine wall-breaking grinding method for salviae miltiorrhizae, and belongs to the field of traditional Chinese medicines. The ultrafine wall-breaking grinding method for salviae miltiorrhizae provided by the invention comprises the following steps: (1) conventionally crushing salviae miltiorrhizae, and filtering through an 80-100mesh sieve to obtain salviae miltiorrhizae powder; (2) mixing sorbitol with phenylalanine evenly, wherein the mass ratio of the sorbitol to the phenylalanine preferably is 1 to 1, and obtaining sorbitol-phenylalanine mixed powder; and (3) mixing the salviae miltiorrhizae powder with the sorbitol-phenylalanine mixed powder evenly, wherein the mass ratio of the salviae miltiorrhizae powder to the sorbitol-phenylalanine mixed powder preferably is (200-100) to 1, crushing the powder until the mean grain size is smaller than 2 microns by an ultrafine grinder, so as to obtain ultrafine salviae miltiorrhizae wall-broken powder. Compared with a conventional ultrafine wall-breaking grinding method, the ultrafine wall-breaking grinding method for salviae miltiorrhizae provided by the invention has the advantages of short processing time, high powder extraction rate, high retention rate and dissolution rate of tanshinone IIA and salvianolic acid B, and small mean grain size of powder.

The invention discloses a gene for generating related diterpene synthase together with a tanshinone compound as well as an encoding product and an application thereof. The gene is named as the tanshin ent-kaurene synthase gene (SmKSL) and is obtained from tanshin by cloning and adopting a gene chip technology, and as for the sequence, see to SEQ ID No.1. The protein encoded by the SmKSL is the protein provided with an aminoacid residue sequence with SEQ ID No.2 or is the protein derived via the SEQ ID No. 2 by replacement, deletion or addition of one or more aminoacid residues for the aminoacid residue sequence with SEQ ID No.2 and provided with the same activity with the aminoacid residue sequence with SEQ ID No.2. The SmKSL is a key enzyme gene in a tanshin diterpene secondary metabolic pathway, the genetic expression is closely related with the generation of the tanshinone compounds, for example, tanshinone IIA, and the invention has important theoretical and practical significances for adjusting and producing plant diterpenoid compounds and cultivating the excellent medical new plant variety.

Disclosed is a medicinal composition for treating senile dementia, wherein 1g of the dried active sites comprises tanshinone IIA 1.79-9.01mg, salvianolic acid B 35.87-225.23mg, danshensu 4.93-45.05mg, protocatechuic aldehyde 0.10-4.50mg, and notoginseng saponin R 13.14-22.50mg.

The invention relates to an antibacterial tanshinone extract extracted from Salvia spp. roots, a preparation method thereof, application of the antibacterial tanshinone extract and products of the antibacterial tanshinone extract. The extract contains cryptotanshinone, dihydro-tanshinone, tanshinone I and tanshinone IIA, wherein the total weight content of the four compounds is 40 to 99 percent of the total weight content of the extract; the weight content of the cryptotanshinone is 50 to 95 percent of the total weight of the four compounds; the weight content of the dihydro-tanshinone is 5 to 30 percent of the total weight of the four compounds; the weight content of the tanshinone I is 0 to 20 percent of the total weight of the four compounds; and the weight content of the tanshinone IIA is 0 to 40 percent of the total weight of the four compounds. The preparation method comprises the steps of diacolation extraction and purification for two times. The extract is used for preparing antibacterial medicines or other products. The tanshinone extract obtained has high purity, more reasonable proportions of various compositions, and can exert better clinic effect.

Generally, tanshinone IIA, cryptotanshinone, dihydro tanshinone and tanshinone I are obtained through column chromatography, which has too lower content of effective component in the product, and is time consuming and material consuming. The present invention provides tanshinone IIA, cryptotanshinone, dihydro tanshinone and tanshinone I extracting process including column chromatography and simultaneous extraction with ethyl acetate, silica gel H, benzene, chloroform and 60-90# petroleum ether, and the extracting process has effectively raised content of effective component in the product.

The invention discloses a tanshinone IIA derivative. A connecting arm, phosphocreatine and an analogue of the phosphocreatine form a conjugate of which the structure is as shown in formula (I) in the specification, wherein n is equal to 1-3, m is equal to 0-3, R1 and R2 respectively independently represent hydrogen atoms, C1-C5 alkyl, cycloalkyl, vinyl and alkynyl, the R1 and the R2 also can form ternary to hexatomic rings, and M represents metal ions, like sodium ions. The compound can be used for improving the water solubility of the whole molecule through sodium phosphate in the phosphocreatine on the premise of maintaining the activity of the tanshinone IIA; moreover, a phosphocreatine fragment in the molecule can play a dual protection effect on myocardial muscles.

A quality control method for the Chinese medicine to treat thrombus features that a thin-layer chromatography is used for the identification to earthworm, vomiting nut, musk, corydalis tuber, toad, liquorice root, etc and an efficient liquid-phase chromatography is used to measure the contents of tanshinone IIA and strychnine.

A process for extracting and refining tanshinone IIA by supercritical CO2 includes loading pwodered red sage root in extractor, extracting at 32-80 deg.C and 30-45 MPa, rectifying the supercritical CO2 fluid in which the extract is dissolved at 25-75 deg.C and 7.5-32 MPa, and separating at 15-75 deg.C and 6.5-25 MPa. Its advantages are high purity up to 80-95% and output rate up to 90% or more, not degradating easily and cyclic use of CO2.

The invention relates to a traditional Chinese medicine composition for treating insomnia and a preparation method and a quality control method thereof. The medicine composition comprises mother-of-pearl, Polygonum multiform, glossy privet fruit, Salvia miltiorrhiza and other bulk medicines. The preparation method is easy, simple and convenient, and can preserve effective components in the medicines and give play to the treatment effect of the medicines to the maximum degree. The quality control method comprises the steps of thin-layer identification of the Salvia miltiorrhiza, Schisandra chinensis, prepared rehmannia rhizome, the glossy privet fruit and Silktree Albizzia barks, content inspection of an ethanol extract, and content assay of an effective component tanshinone IIA in the medicines, and can effectively control the quality of the medicines and guarantee the stability of the treatment effect of the medicines.

The invention provides a method for using a near infrared spectroscopy to measure contents of multiple components in a radix et rhizoma salviae miltiorrhizae medicinal material and application feasibility of the method in a Chinese patent medicine raw material quality assurance system is discussed. The method comprises the steps: utilizing a high performance liquid chromatography to measure contents of danshinolic acid B, rosmarinic acid, alkannic acid, tanshinone IIA, tanshinone I and cryptotanshinone in radix et rhizoma salviae miltiorrhizae as contrast values; utilizing near infrared diffusion reflection method to collect a sample spectrum, comparing different spectrum pretreating methods and modeling effects of modeling waveband and optimizing modeling parameters; utilizing a partial least squares (PLS) to respectively establish near infrared quantitative analysis models of danshinolic acid B, rosmarinic acid, alkannic acid and tanshinone components (tanshinone IIA plus tanshinoneI plus cryptotanshinone) in the radix et rhizoma salviae miltiorrhizae and evaluating the models. The quantitative analysis models established by the method disclosed by the invention can meet a requirement of quickly obtaining chemical information of massive radix et rhizoma salviae miltiorrhizae medicinal material samples.

The invention is applicable to the field of medical chemistry and provides a natural and edible composition with healthcare functions, application of composition, makeup cosmetics and a preparation method of the makeup cosmetics. The composition comprises multiple active molecular tanshinone IIA, a ginseng extract and soybean lecithin which are natural, all the components have an effect synergistic superposition function, have significant therapeutic effects, are natural, edible, safe and free of side effects, can be applied to preparation of makeup cosmetics with multi-molecular, multi-target, multi-layer, multi-link and multi-way functions, including but not limited to lipsticks, lip cream, lip gloss, colored lip cream, lip balm, lip moisturizing gel, lip protection oil, lip glaze, lip dying liquids, lip liners, rouge, eye shadow, blusher, pressed powder, liquid foundation, BB cream, air cushion cream, eye liner, eye shadow cream, eyebrow pencils and finishing powder, and can endow the cosmetics with multiple skin care and healthcare effects so as to enrich functions of the cosmetics.

The invention relates to a Chinese herbal medicine compound preparation for treating skin and mucous membrane disease. The Chinese herbal medicine compound preparation is prepared from the following raw materials: cortex moutan, polygonum chinense, radix salviae miltiorrhizae, herba lycopi, common dayflower herb, rhizoma smilacis glabrae, ramulus cinnamomi, caulis dendrobii officinalis, gorgon fruit and cortex cinnamomi; the ingredient of the Chinese herbal medicine compound preparation comprises a cortex moutan extract, a polygonum chinense extract, a radix salviae miltiorrhizae extract, a herba lycopi extract, a common dayflower herb extract, a rhizoma smilacis glabrae extract, a ramulus cinnamomi extract, a caulis dendrobii officinalis extract, a gorgon fruit extract and a cortex cinnamomi extract; and the effective ingredient of the cortex moutan extract is paeonol, the effective ingredient of the polygonum chinense extract is quercitrin, the effective ingredient of the radix salviae miltiorrhizae extract is tanshinone IIA, the effective ingredient of herba lycopi is ursolic acid, the effective ingredient of common dayflower herb is commelinin, the effective ingredient of rhizoma smilacis glabrae is astilbin, the effective ingredient of ramulus cinnamomi is cinnamaldehyde, the effective ingredient of caulis dendrobii officinalis is erianin, the effective ingredient of gorgon fruit is cyclo(profilum), and the effective ingredient of cortex cinnamomi is cinnamaldehyde.

The invention provides a drug composition for treating cardio-cerebrovascular diseases and a preparation method. The drug composition is jointly formed by such active ingredients as 2-10 parts by weight of salvianolic acid B, 0.1-0.5 part by weight of tanshinone IIA, 0.1-0.5 part by weight of ferulic acid, 0.4-2 parts by weight of ligustilide and 2-10 parts by weight of puerarin and the auxiliary added ingredients acceptable in the drugs. Tests show that the drug composition can obviously reduce brain tissue hemorrhagic necrosis caused by brain ischemia reperfusion injury and alleviate injuries of nerve cells and nerve fibers.

The solution of sample is prepared according to following steps. Powder of red sange root 0.3 g is fetched with methanol 50ml being added. Then, ultrasound operation is carried out for the solution just prepared. Cooling the preparing solution to room temperature and shaking up obtains the solution of sample. Reference substance is prepared according to following steps. fetching an amount of tanshinone IIA with methanol being added produces the solution of reference substance (20 ª–g/ml). The standard finger print of fat soluble component of red sange root is obtained through measurement and calculation by using high performance liquid chromatograph for 5 ª–L of sample solution and 5 ª–L of reference substance. The method provides the advantages of simple, stable, high accuracy and good repeatability. Thus, the invention can be used to control quality and distinguish whether the product is true or false.