58 results about "Dihydrotanshinone" patented technology

The invention relates to a method for extracting tanshinone which comprises the following two steps: first, alcohol extraction process: separating the extraction liquid and dregs with the danshen liposolubility content from danshen medicine materical crude slices or disintegrated danshen medicinal materials by bath method, hypersonic extraction or microwave extraction in condition of normal temperature or heating, and reserving, adding correct concentration alcohol into the dregs, inverse flow extracting with heating to get the extraction, which combined with the extraction get from the first extraction, removing the solid impurities by the methods of filtration, centrifugation or deposition, and reserving. Second, isolation method separation: adding the medicine liquid get from the said alcohol extraction process to a macroreticular resinous adsorbent column, gradient eluting by alcohol with a finite concentration respectively to get the extraction I (with cryptotanshinone and dihydrotanshinone I) and extraction II (with tanshinone IIA and tanshinone I).

The invention relates to tanshinone compounds in the technical field of medicine and applications thereof. The tanshinone compounds contain tanshinone I, tanshinone IIA, cryptotanshinone and dihydrotanshinone I and the structural formulas are shown as below. The method of the invention uses the tanshinone compounds as the active ingredients of the androgen receptor antagonist; the compounds are used alone or used along with the excipient to prepare oral agents or non-oral agents for curing the diseases related to androgen.

The invention relates to an antibacterial tanshinone extract extracted from Salvia spp. roots, a preparation method thereof, application of the antibacterial tanshinone extract and products of the antibacterial tanshinone extract. The extract contains cryptotanshinone, dihydro-tanshinone, tanshinone I and tanshinone IIA, wherein the total weight content of the four compounds is 40 to 99 percent of the total weight content of the extract; the weight content of the cryptotanshinone is 50 to 95 percent of the total weight of the four compounds; the weight content of the dihydro-tanshinone is 5 to 30 percent of the total weight of the four compounds; the weight content of the tanshinone I is 0 to 20 percent of the total weight of the four compounds; and the weight content of the tanshinone IIA is 0 to 40 percent of the total weight of the four compounds. The preparation method comprises the steps of diacolation extraction and purification for two times. The extract is used for preparing antibacterial medicines or other products. The tanshinone extract obtained has high purity, more reasonable proportions of various compositions, and can exert better clinic effect.

Generally, tanshinone IIA, cryptotanshinone, dihydro tanshinone and tanshinone I are obtained through column chromatography, which has too lower content of effective component in the product, and is time consuming and material consuming. The present invention provides tanshinone IIA, cryptotanshinone, dihydro tanshinone and tanshinone I extracting process including column chromatography and simultaneous extraction with ethyl acetate, silica gel H, benzene, chloroform and 60-90# petroleum ether, and the extracting process has effectively raised content of effective component in the product.

The invention relates to a rapid separation liquid chromatography detection method for naoxintong capsules. The method comprises the chromatographic conditions that octadecyl bonded silica gel columns serve as filling agents, 0.1% formic acid water serves as an A mobile phase, acetonitrile serves as a B mobile phase, the volume ratio of the A mobile phase to the B mobile phase is 10-85:15-90, and linear gradient elution is performed, the flow velocity is set at 0.20 mL.min<-1>, the column temperature is set at 25 DEG C, and the sample introduction volume is 5 microliters. The content of gallic acid, tanshinol, hydroxysafflor yellow A, chlorogenic acid, amygdalin, protocatechualdehyde, epicatechin, caffeic acid, albiflorin std, ononin, paeoniflorin, rutin, salvianolic acid A, cinnamic acid, fermlononetin, dihydrotanshinone I in the naoxintong capsules is detected. The detection method has the advantages of being rapid, stable and accurate.

The invention discloses a coding gene sequence of an AP2 / ERF transcription factor SmAP2 / ERF82 for regulating tanshinone synthesis. The SmAP2 / ERF82 gene provided by the invention has a nucleotide sequence as shown in the SEQ ID No.1. The gene encoded protein has an amino acid sequence as shown in the SEQ ID No.2. A SmAP2 / ERF82-RNAi vector and a SmAP2 / ERF82-overexpression vector are constructed in the invention. Through a genetic transformation method for transformation of red sage root, transgenic hairy roots are obtained. In comparison with a control line, contents of dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone II A in the SmAP2 / ERF82-RNAi line are remarkably reduced, and contents of dihydrotanshinone I and cryptotanshinone in the SmAP2 / ERF82-overexpression line areremarkably raised. The SmAP2 / ERF82 provided by the invention has the function of positive regulation of tanshinone compound biosynthesis. The compounds have remarkable efficacy of treating cardiovascular diseases. The invention brings forth new ideas for the research on molecular mechanism for tanshinone synthesis regulation and lays a foundation for the biological research on synthesis of tanshinone.

A composition comprising tanshinone compounds selected from the group consisting of miltirone, 1,2-didehydromiltirone, tanshinone IIA, tanshinone I and dihydrotanshinone I isolated from Salviae miltiorrhizae Bunge showing potent inhibiting effect on the aggregation and toxicity of beta-amyloid and recovering activity of memory learning disorder confirmed by Y maze study and Passive Avoidance study. Therefore it can be used as the therapeutics or health food for treating and preventing cognitive function disorder with safe.

The invention relates to the field of natural medicaments, in particular to a diterpenes compound in a radix salviae miltiorrhizae extract, mainly relates to the expression of a tanshinone compound in a reversal benzoapyrene-induced CYP1 enzyme, as well as application in the direct inhibition of the CYP1 enzyme activity. The invention discloses new pharmacological application of four types of tanshinones including tanshinone I, tanshinone II A, cryptotanshinone and dihydrotanshinone I, namely the four tanshinones not only can directly inhibit the activities of various subtype enzymes in the CYP1 family but also can reverse the up-regulation of a benzoapyrene-induced CYP1 function in Hep-G2 cells. The tanshinone compound has the development application prospect that the tanshinone compound can be developed into a supplementary drug for the treatment of cancer as a multi-drug resistant reversal agent and an anti-cancer health care product.

The invention discloses a method for extracting and preparing tanshinone compounds from dregs of radix salviae miltiorrhizae by virtue of a saccharification pretreatment method. The method comprises the steps of firstly carrying out saccharification treatment on waste dregs of radix salviae miltiorrhizae to damage cell wall cellulose, hemicelluloses and lignocelluloses, extracting treatment liquid by virtue of an organic solvent, concentrating extraction liquid to a certain volume, carrying out ethanol elution on a macroporous resin column, collecting optimized eluate parts, concentrating, and carrying out spray drying, so as to prepare chemical components of tanshinone. According to the method, the extraction rate can reach 80%-100%, and the purity can reach 70%-95%; the content of tanshinone IIA can reach 36%, the content of tanshinone I can reach 32%, the content of cryptotanshinone can reach 8%, the content of dihydrotanshinone can reach 9%, and the content of active ingredients is high. Experiment results prove that the tanshinone compounds have well efficacies of promoting blood circulation and removing stasis and can be used for preparing medicines or functional health products capable of preventing and treating cardiovascular and cerebrovascular diseases.

The invention relates to a method for detecting tanshinone components, and an application thereof. The tanshinone components are dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA. The method comprises the following steps: collecting samples to be tested in the extraction and concentration of Salvia miltiorrhiza, collecting the near infrared spectra of the samples to be tested bynear-infrared spectroscopy, and converting the near infrared spectra into the respective content of dihydrotanshinone, cryptotanshinone, tanshinone I and tanshinone IIA in every sample to be tested according to a selected model. The method has the advantages of fastness in detection of the respective content of the tanshinone components in a compound Salvia miltiorrhiza tablet, simplicity in thepreparation of the samples, and realization of online analysis.

The invention discloses an application of a salvia miltiorrhiza extract and quinone compounds in resisting coronavirus. One or more compounds of tanshinone I, tanshinone IIA, cryptotanshinone, dihydrotanshinone I, beta-lapamoquinone, L-shikonin, 2-methoxy-1, 4-naphthoquinone, CPI-169, S3I-201 and plumbagin, pharmaceutically acceptable salts thereof, solvates thereof, solvates of the pharmaceutically acceptable salts thereof, or crystal forms thereof are applied in preparation of anti-coronavirus agents, medicines for treating and / or preventing diseases caused by coronavirus, and / or an inhibitor for inhibiting papaya-like protease. The compounds can well inhibit the activity of the papaya-like protease in the coronavirus and inhibit the replication ability of the coronavirus in cells so as to inhibit the activity of the coronavirus, and when the compounds are used for treating diseases caused by the coronavirus, the toxic and side effects are low, and oral administration is easy to implement.

The invention relates to a double-column circulating separation system and method for preparing a high-purity tanshinone compound. The double-column circulating separation system comprises three counter-current chromatographic columns, three pumps, three detectors, three collectors and three six-way valves. A first counter-current chromatographic column serves as an initial separation column, a second counter-current chromatographic column and a third counter-current chromatographic column serve as mutually independent circulating separation columns, and the multidimensional circulating separation of the tanshinone compound can be realized by adjusting a second six-way valve and a third six-way valve. The tanshinone compound comprises dihydrotanshinone I, triiuganone B and methyl tanshinone ester. The system and method disclosed by the invention are simple in extraction and separation, short in time, high in product purity, capable of being continuously operated, and suitable for industrial production.

The invention relates to the field of drug compound detection, in particular to a method for detecting tanshinone compounds in compound salvia tablets. A plurality of components of the same type including dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA can be simultaneously detected by detecting the index component of the tanshinone IIA. The method is simple and convenient, is fine in universality and can be used for more completely reflecting the quality of the compound salvia tablets.

The invention relates to the field of natural medicines, and discloses application of dihydrotanshinone I in preparation of medicines for treating multi-drug resistant tumors as well as a preparationmethod and a pharmaceutical composition of the dihydrotanshinone I. The dihydrotanshinone I disclosed by the invention has a remarkable inhibiting effect on drug-resistant tumor cells; the mechanism of the dihydrotanshinone I is that the killing of multi-drug resistant tumor cells is not affected by P-glycoprotein, and the intracellular drug concentration can be maintained at a normal level, so that the multi-drug resistance of tumors can be overcome. Furthermore, the killing effect of the dihydrotanshinone I on the multi-drug resistant tumor cells is significantly better than the representative antitumor drugs which are commonly used at present. The mechanism of the dihydrotanshinone I killing P-glycoprotein high-expression multi-drug resistant tumor cells is to induce apoptosis, and is consistent with parental drug-sensitive cells; furthermore, the dihydrotanshinone I has lower toxicity; therefore, the dihydrotanshinone I has a good clinical application prospect for the multi-drug resistant tumors.

The invention relates to a single-column cyclic separation system for preparing high-purity tanshinone compounds and a method thereof. The single-column cyclic separation system comprises three countercurrent chromatographic columns, three pumps, three detectors, three collectors and three six-way valves. The first countercurrent chromatographic column is taken as a starting separation column, the second countercurrent chromatographic column and the third countercurrent chromatographic column are used as mutually independent cyclic separation columns, the second six-way valve is used for connecting the first and second countercurrent chromatographic columns, and the third six-way valve is used for connecting the first and third countercurrent chromatographic columns; the multi-dimensional cyclic separation of tanshinone compounds can be realized by regulating the second six-way valve and the third six-way valve. The tanshinone compounds comprise dihydrotanshinone I, triiuganone B, methyl tanshinone ester, cryptotanshinone, tanshinone I and 1, 2-dihydrotanshinone. The single-column cyclic separation system and the method, provided by the invention, have the advantages of simple extraction and separation, short time, high product purity and continuous operation, and are suitable for industrial production.

The invention provides a method for detecting a compound salvia miltiorrhiza tablet, which comprises the following steps of: providing a test sample solution of a compound salvia miltiorrhiza tablet to be detected; selecting one of dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA and borneol for dissolving in ethyl acetate to be used as a contrast sample solution; and detecting the test sample solution by using a mixture of petroleum ether and acetone as a developing solvent through adopting a thin-layer chromatography. In the invention, the mixture of the petroleum ether and the acetone is used as the developing solvent; compared with the benzene-contained developing solvent used in the prior art, the developing solvent used by the invention has no poison; and in addition, a detecting result indicates that the developing solvent used by the invention can accurately detect active components of the compound salvia miltiorrhiza tablet.

The invention provides effective components of radix salviae miltiorrhizae, mainly comprising TrijuganoneC as A compound, 7-Hydroxy-8, 13-abietadiene-11, 12-dione as B compound and Dihydrotanshinone I as C compound with respective content of 40 to 50 percent, 30 to 40 percent and 3 to 10 percent. The effective components can be obtained through following procedures: heating extraction, condensing, silica gel column separation, elution, condensing and drying of the eluted solution, liquid chromatography separation, solution collecting and condensing and drying of the solution. The effective components can be used in the preparation of drugs for treating and preventing tumors. With reasonable design, the invention can be quickly obtained and can be more favorable for quality control of the drugs.

The invention belongs to the field of medicines, and relates to application of dihydrotanshinone I in preparation of anti-hepatic fibrosis medicines. Systematic researches on the inhibiting effect on the mRNA and protein level of fibrosis main markers (COL1A1, TGFB1, ACTA2 and MMP2) in a TGF beta 1 induced LX-2 cell, influence on contents of ALT and AST in the serum of a BDL rat, improvement on the pathological change of liver tissues of the BDL rat, influence on the content of hydroxyproline of liver tissues of the BDL rat, inhibition on the mRNA and protein level of fibrosis main markers of liver tissues of a rat, influence on the hepatic fibrosis degree of the rat prove that the dihydrotanshinone I has excellent anti-hepatic fibrosis activity, and is expected to be developed into novel anti-hepatic fibrosis medicines.

The invention relates to a tanshinone class I derivant and a synthesizing method and an application thereof, wherein the tanshinone class I derivant is characterized in that various substituent groups are introduced into different positions of a ring A of a tanshinone I molecule, the number of carbonyl groups on a ring C of the tanshinone I molecule is selectively retained, and a ring D of the tanshinone I molecule is reduced into a dihydrofuran ring. The preparation method comprises the steps that Diels-Alder reaction is carried out on a styrene compound and p-benzoquinone, and a key skeleton--phenanthraquinone compound is obtained after separation and purification; and then the phenanthraquinone compound is reacted with enamine to introduce a furan ring into the phenanthraquinone skeleton, and a target product--tanshinone class I compound is obtained through acidizing hydrolysis, oxidation reaction, separation and purification, and the tanshinone class I compound is catalytically hydrogenated to obtain a dihydro tanshinone class I compound. Cytotoxicity research and pharmacology research results show the cytotoxicity of the compound, so that the tanshinone compound has wide clinical application prospects, and is expected to be used as a new antineoplastic medicine.

This invention relates to the use of a plant extract, derived from a Salvia spp, comprising one or more tanshinone compounds, or said one or more tanshinone compounds, in the treatment of wounds, particularly chronic wounds, or other conditions benefiting from inhibition of Cortisol production, particularly Cushing's syndrome. Preferred tanshinone compounds include, but are not limited to, dihydrotanshinone (particularly 15,16- dihydrotanshinone (CAS No. 87205-99-0) ) and Tanshinone I.

The invention discloses a pharmaceutical composition for treating myocardial infarction. The pharmaceutical composition comprises a tanshinone compound shown in a formula (I) and a phenolic acid compound containing one or more substituent shown in a formula (II), preferably, comprises tanshinone I and tanshinol or tanshinone IIA and salvianolic acid B or dihydrotanshinone I and protocatechualdehyde or cryptotanshinone and salvianolic acid C. According to the invention, the phenolic acid compound containing the specific substituent and the tanshinone compound having a specific structure are prepared to obtain the pharmaceutical composition. The pharmaceutical composition can effectively reduce the myocardial infarction area of mice with acute myocardial infarction, and has low toxic and side effects; the phenolic acid compound in the composition can obviously reduce the in vitro and vivo toxic and side effects of the tanshinone compound, and can enhance the cardiac muscle protection effect of the tanshinone compound on the mice with acute myocardial infarction.

The invention belongs to the field of acetylcholinesterase inhibitor screening, and particularly relates to a composition, a preparation method and an application of the composition as an acetylcholinesterase inhibitor. The existing research shows that danshen roots and danshen root Chinese patent medicine have good anti-Alzheimer disease effect, and the full screening of potential acetylcholinesterase inhibitor in the danshen roots has important significance. The invention provides a method for separating and preparing compounds with anti-acetylcholine activity in the danshen roots by combining ultrafiltration affinity and semi-preparative liquid chromatography, the method screens out six compounds of Oleoyl neocyptotanshinone, dihydrotanshinone I, cryptotanshinone I, tanshinone I, tanshinone II A and Miltirone from an ethyl acetate extract of the danshen roots, and the compounds have good acetylcholinesterase inhibition activity. The method provides technical support for rapidly screening out the acetylcholinesterase inhibitor, and the research result has guiding significance for the development of medicines for treating Alzheimer disease.

The invention relates to a method for detecting active ingredients of salviae miltiorrhizae and products. The method comprises the following steps: preparation of a test solution, preparation of a reference solution and sample detection. In the method provided by the invention, index ingredients such as sodium danshensu, rosmarinic acid, alkannic acid, danshinolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone II contained in salviae miltiorrhizae and preparations thereof are simultaneously detected for the first time to realize more comprehensive evaluation and control of the quality of salviae miltiorrhizae and preparations thereofs. The detection time is shortened, the detection cost is reduced, the method is not only accurate, but also is simple and feasible. The method provided by the invention has good stability, good repeatability, high recovery rate and relatively high specificity.

The invention discloses joint application of dihydrotanshinone I and protocatechualdehyde to preparation of medicines for treating acute myocardial infarction and particularly provides a medicine for treating acute myocardial infarction. Active components of the medicine include dihydrotanshinone I and protocatechualdehyde. A pharmaceutical preparation for treating acute myocardial infarction comprises the active components and a pharmaceutically acceptable carrier or excipient and is made into a pharmaceutically acceptable form. The joint application has advantages that toxicity of dihydrotanshinone I is reduced while efficacy advantages of the dihydrotanshinone I are achieved, namely myocardial infarction area of acute myocardial infarction rats can be effectively reduced, long drug action is realized, and toxic lethality is evidently decreased. Further, treatment effects of the dihydrotanshinone I on the myocardial infarction rats can be evidently improved by addition of protocatechualdehyde in different doses, and the dihydrotanshinone I and the protocatechualdehyde have an efficacy synergistic effect.

The invention discloses a method for preparing tanshinone IIA and dihydrotanshinone I by adopting a medium / high-pressure preparation liquid-phase method. The method comprises the following steps: (1) drying the salviae miltiorrhizae dreg after aqueous extraction, crushing and concentrating with ethanol, thereby acquiring salviae miltiorrhizae alcohol extract; (2) extracting the extract with petroleum ether and ethyl acetate, taking the part extracted with ethyl acetate and concentrating; (3) adopting medium pressure preparation liquid phase, thereby acquiring a total tanshinone crude extract; (4) adopting UPLC analysis and then merging tanshinone IIA and dihydrotanshinone I; (5) performing high pressure preparation liquid phase separation, thereby acquiring tanshinone IIA and dihydrotanshinone I monomeric compounds. According to the invention, the medium / high-pressure preparation liquid-phase combined technique is adopted for efficiently and quickly separating and purifying high-purity tanshinone IIA and dihydrotanshinone I compound from the salviae miltiorrhizae dreg; the waste resources can be fully utilized; the method has environmental protection significance and economic value.

The invention discloses a method for simultaneously and rapidly determining various phenolic acid and tanshinone components in compound salvia milliorrhiza tablets. The method comprises the followingsteps: mixing a product to be detected and an extracting solvent; after carrying out microwave assisted extraction, detecting by a high performance liquid chromatography; quantifying salvianic acid, protocatechuic aldehyde, caffeic acid, rosmarinic acid, lithospermic acid, salvianolic acid A, salvianolic acid B, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA quantitation byadopting an external standard method. Under the condition that the compound salvia milliorrhiza tablets contain various complicated components, the method can be used for simultaneously determining 7phenolic acid components and 4 tanshinone components in the compound salvia milliorrhiza tablets; the quality analysis efficiency of the compound salvia milliorrhiza tablets and the quality control level of a product can be remarkably improved; the method has the advantages that a few of organic solvents are consumed, energy saving and environmental protection are saved, and a green and chemicaldevelopment concept is met.

The invention relates to a method for extracting tanshinone which comprises the following two steps: first, alcohol extraction process: separating the extraction liquid and dregs with the danshen liposolubility content from danshen medicine materical crude slices or disintegrated danshen medicinal materials by bath method, hypersonic extraction or microwave extraction in condition of normal temperature or heating, and reserving, adding correct concentration alcohol into the dregs, inverse flow extracting with heating to get the extraction, which combined with the extraction get from the first extraction, removing the solid impurities by the methods of filtration, centrifugation or deposition, and reserving. Second, isolation method separation: adding the medicine liquid get from the said alcohol extraction process to a macroreticular resinous adsorbent column, gradient eluting by alcohol with a finite concentration respectively to get the extraction I (with cryptotanshinone and dihydrotanshinone I) and extraction II (with tanshinone IIA and tanshinone I).

The invention relates to the technical field of microorganisms, and discloses radix salviae miltiorrhizae endophytic fungi and application thereof to promotion of growth of radix salviae miltiorrhizae / or synthesis of active ingredients. The invention provides the radix salviae miltiorrhizae endophytic fungi of which the preservation name is (phomaherbarum) D603, the preservation number is CGMCC No.16884, and the preservation unit is China center for microbial culture collection management committee general microbiology. Meanwhile, the invention also discloses application of the radix salviae miltiorrhizae endophytic fungi, and thus, the growth of the radix salviae miltiorrhizae can be effectively promoted, a main root of the radix salviae miltiorrhizae is thickened and lateral roots are developed, and the color of the surface of the root turns red; and moreover, the synthesis of tanshinone I, cryptotanshinone, dihydrotanshinone I, tanshinone IIA, tanshinol, rosmarinic acid and salvianolic acid B in the radix salviae miltiorrhizae can be effectively promoted.

A plant extract, derived from a Salvia spp, may include one of at least one tanshinone compound, or at least one tanshinone compounds including a CYP11B1 inhibitory amount of at least one of tanshinone I and dihydrotanshinone. The plant extract may be used for use in the treatment of a wound or Cushing's syndrome.