Rapid separation liquid chromatography detection method for naoxintong capsules

Abstract

The invention relates to a rapid separation liquid chromatography detection method for naoxintong capsules. The method comprises the chromatographic conditions that octadecyl bonded silica gel columns serve as filling agents, 0.1% formic acid water serves as an A mobile phase, acetonitrile serves as a B mobile phase, the volume ratio of the A mobile phase to the B mobile phase is 10-85:15-90, and linear gradient elution is performed, the flow velocity is set at 0.20 mL.min<-1>, the column temperature is set at 25 DEG C, and the sample introduction volume is 5 microliters. The content of gallic acid, tanshinol, hydroxysafflor yellow A, chlorogenic acid, amygdalin, protocatechualdehyde, epicatechin, caffeic acid, albiflorin std, ononin, paeoniflorin, rutin, salvianolic acid A, cinnamic acid, fermlononetin, dihydrotanshinone I in the naoxintong capsules is detected. The detection method has the advantages of being rapid, stable and accurate.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a rapid separation liquid chromatography detection method for Naoxintong capsules. Background technique [0002] Naoxintong Capsules are exclusively produced by Shaanxi Buchang Pharmaceutical Co., Ltd., and the quality standards are currently implemented. In 2002, the State Drug Administration compiled "National Standards of Chinese Proprietary Medicines - National Standards of Chinese Proprietary Medicines". "Internal Medicine-Brain Department" Fascicle, standard number: WS-10001(ZD-0001)-2002), has the effects of nourishing qi and activating blood circulation, removing blood stasis and dredging collaterals. It is used for apoplexy caused by qi deficiency and blood stagnation, venation stasis, hemiplegia, numbness of limbs, crooked mouth and eyes, strong tongue, lingering speech, chest pain, chest tightness, palpitations, shortness of breath; cerebral infarction, coro...

AI Technical Summary

Problems solved by technology

It is processed from 16 traditional Chinese medicines including astragalus, salvia, angelica, chuanxiong, red peony, safflower, frankincense (made), myrrh (made), mulberry branch, cinnamon branch, achyranthes bidentata, peach kernel, scorpion, earthworm and leech , Chinese patent document CN103207255A discloses the quality control method of the composition, adopts ultra-high performance liquid chromatography (UPLC) technology to simultaneously measure hydroxysafflor yellow A, paeoniflorin, ferulic acid, and salvianolic acid B in Naoxintong capsules , 5 main chemical components of ligustilide, the above methods are difficult to fully characterize the physical and chemical characteristics of Naoxintong

Moreover, because Naoxintong Capsules has good clinical effects and has a broad market prospect, the current quality standard of the preparation only controls the content of paeoniflorin, and the complexity of the chemical components in Naoxintong Capsules, the existing detection technology There are many problems such as difficult separation and long analysis time, which are obviously not enough to control the internal quality of Naoxintong capsules.

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