1200 results about "Phenolic acid" patented technology

This invention concerns the method of extracting salvianolic acid A from Chinese crude drug: danshen root, and the quality control methods and drug combinations, and the application of this drug. It can be used in the preparation of the prevention drugs for cardiovascular disease, liver damage, liver fibrosis, pulmonary fibrosis and other.

A process for preparing danshinolic acid A from red sage root includes such steps as extracting in water or alcohol, concentrating, high-temp and -pressure reaction, purifying by chromatographic column, regulating pH value, extracting in organic solvent, concentrating and drying.

The present invention provides a technique method which separates lignose, hemicellulose, cellulose and phenolic acid from lignocellulosic biomass. Under the selected pH value and temperature, the lignocellulosic biomass is processed by hot water, so that the hemicellulose and the phenolic acid are preferentially resolved in aqueous solution, delignification processing is carried out, organic solvent or alkaline solution is applied to carry out high-temperature cooking, the generated liquid-solid phase product can be easily and effectively separated, and thus the hemicellulose, the cellulose and the phenolic acid can be respectively obtained. Moreover, enzymatic treatment can be selectively added, and a solid phase product is obtained at the first stage of a group of enzymatic treatment with a cooperative effect. Because the ferulic acid linkage between the hemicellulose and the lignose can be selectively broken, the separation of the chemical components has a high yield rate and high purity.

The invention discloses a method for controlling the quality of ginkgo leaves and an extract thereof. After the screening of a great quantity of experiments, the method adopts ultra-high performance liquid chromatography for detection; and the method can be used for detecting three types of components including flavone, terpene lactones and phenolic acid, twenty-four active compounds in total, in the ginkgo leaves and the extract of the ginkgo leaves. The results of the experiments prove that the method is high in detection sensitivity and good in stability, and can be used for objectively, comprehensively and accurately evaluating the quality of ginkgo leaf medicines, the extract of the ginkgo leaves and ginkgo leaf preparations, thus having important significance on controlling the quality and guaranteeing the clinical treatment effect.

A purified cannabidiol (CBD) extract and/or cannabidiolic acid (CBDA) extract is isolated from industrial hemp and comprises less than 0.5 wt % organic impurities as measured by HPLC and ¹H NMR spectroscopy exhibits no detectable peak at 4.07 ppm as measured by ¹H NMR spectroscopy. The CBD and/or CBDA extract is in crystalline form. The CBD extract exhibits a melting point as measured by differential scanning calorimetry (DSC) of 69-70° C. Dry powder compositions comprise such extracts. Additional dry powder compositions comprise polyvinylpyrrolidone and an amorphous CBD extract. An adduct comprises CBD and/or CBDA bonded to a paramagnetic trivalent lanthanide (III) metal chelate.

The present invention relates to processes for the treatment of liquors derived from oil-bearing fruit, for example from oil palm fruit, and to products therefrom. Typically a process involves removal of undissolved solids, oleaginous parts, colloids and higher weight molecules from the vegetation liquor to give an aqueous fraction containing phytochemicals, for example, flavonoids, phenolic acids and hydroxy acids. Subsequently, pH adjustment and solvent extraction upon said aqueous fraction realise an extract rich in hydroxy acids or phenolic acids or flavonoids or any combination thereof Applications of the substances subject of this invention are to be found in drinks, edible products, tonics, health supplements, antioxidant additives, cosmetics, soaps, shampoos, detergents, drugs or medicinal products.

A process for extracting danshinolic acids A and B from red sage root includes soaking in hot water, collecting liquid extract, centrifugal separating, ultrafiltering of supernatant, extracting by reverse-phase column or acetate, acidifying, separating organic phase to recover solvent, and separating by reverse-phase column. A freeze dried injection for preventing and treating liver injury and hepafibrosis is prepared from said danshinolic acids A and B and additives. Its advantages are high purity and high output rate.

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.

The present invention relates to a compound salvia active component group and its slow-release preparation, medicinal application and preparation method. It features convenient administration and good stability. Said invention contains salvia active component (salvia total phenolic acid content is above 50%), notoginseng active component (notoginseng total saponin content is above 50%) and natural (or artifiical) borneol and its cyclodextrin inclusion. It can be made into various slow-release preparations, and is obtained by using water or pure water to make extraction, dissolving in water, removing impurity by using water to wash and recovering and drying. It can be used for making medicine for curing angiocardiopathy and cerebrovascular diseases.

The invention discloses a method for manufacturing salvianolic acid A of danshen root, which is characterized in that total salvianolic acid in the danshen root is transformed into salvianolic acid A by adopting a certain method. The method increases greatly the extraction yield of salvianolic acid A. The salvianolic acid A is prepared into tablets, capsule, granula, soft capsule, micro pill, dripping pill and oral liquid, which have good pharmacological action indicated by pharmacological experiments.

There is provided a surfactant composition, a stable, self emulsifiable water borne curing agent composition, and methods for the manufacture of each, and two component water borne curable epoxy resin compositions. The surfactant composition comprises the reaction product of an a) a phenolic acid substituted with at least one carboxyl group and at least one hydrocarbyl group having at least 1 carbon atom; b) a polyepoxide compound; c) a polyamine compound having at least two primary amine groups; d) a reactive surfactant; and optionally e) a monoglycidyl, monocarboxylic acid, or monoisocyanate capping agent; wherein the reactive surfactant comprises an epoxide, a carboxylic acid or anhydrides thereof, or an isocyanate functional moeity, and a hydrophilic moiety comprising a polyoxyalkylene monool or polyol residue. The reactive surfactant comprises a compound represented by one of the following formulas:

The invention relates to a DanShen fingerprint spectrum quality control method, comprising that (1), adding 1.0g DanShen powder into carbinol to extract via microwave, (2), washing flow phase gradient that the Alltima C18 column is 4.6mm, 250mm, and 5mum, checking wavelength is 280nm, flow speed is 1.0ml/min, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is DanShen element, the eighth peak is rosmarinic acid, the ninth peak is alkannic acid, the tenth peak is phenolic acid B, the eleventh peak is phenolic acid B isomer, the fourteenth peak is cryptotanshinone and the fourteenth peak is tanshinone IIA, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 0.21 of DanShen element, 0.91 of rosmarinic acid, 0.93 of alkannic acid, 1.00 of phenolic acid B, 1.04 of phenolic acid B isomer, 0.92 of cryptotanshinone and 1.00 of tanshinone IIA, (5), the DanShen planting collecting method. The invention can control the quality of materials to assure the stable quality of product.

The invention relates to a method for ultrasonically extracting and preparing flavonoids pigment from chrysanthemum, belonging to the field of food processing and especially used for deep processing chrysanthemum. The process comprises the following steps of: blanching, drying and hermetically storing chrysanthemum at a low temperature, filtering by using a 100-mesh sieve, soaking in 60% ethanol as an extraction solvent in a liquid-material ratio of 40/1 (v/w) for 2 h, ultrasonically extracting a dry base in a water bath at 70 DEG C for 10 min to acquire the yield of 13.34 percent; and evaporating in the water bath at 70 DEG C by using the 60% of ethanol extraction liquid in a rotating way to release an ethanol taste; and freezing and drying in vacuum, wherein analytical compositions reach 52.01% of the dry base and comprise flavonoids, linarin, flavonoids (luteolin, acacetin and apigenin), flavonols (quercetin), phenolic acid (chlorogenic acid and caffeic acid), metal elements, and the like. The invention provides a new method for deep processing the chrysanthemum and has strong maneuverability, good safety and good repetitiveness.

The invention discloses a golden fungus gingko yellow wine. Alcoholic strength is 8-45%, general flavone content is more than or equal to 1-5 mg/L, ginkgolide is more than or equal to 1-6 mg/kg, total triterpenoid compound content is 30-800 mg/L, protein is more than or equal to 60 mg/kg, soluble sugar is less than or equal to 2.0%, gingko phenolic acid is less than or equal to 5 ppm, and hydrocyanic acid is less than or equal to 0.1 mu g/L. A double-enzyme method is adopted to hydrolyze gingko to obtain gingko hydrolysate which contains active compounds, such as ginkgo flavone, triterpenoid compound, amino acid, peptide, starch and the like, wherein the content of amino acid and peptide is more than or equal to 1%, and starch content is more than or equal to 2%. After being added with gingko leaf extract, the hydrolysate is converted by golden fungus strains, and rice koji is added to obtain fermentation liquor; the fermentation liquor is filtered and blended to obtain golden fungus gingko yellow wine. The wine contains gingko and golden fungus active ingredients and has the efficacy of preventing diabetes and hyperlipidemia, preventing oxidation and improving immunity of body.

The invention relates to a method for increasing the contents of tanshinone and salvianolic acid in a salvia miltiorrhiza hairy root by using a transgene AtMYC2, belonging to the technical field of gene engineering. The method comprises the steps of constructing a high-efficiency expression vector of a plant by using an arabidopsis transcription factor AtMYC2, and carrying out genetic transformation on salvia miltiorrhiza leaves to obtain a gene AtMYC2 overexpressed transgenetic salvia miltiorrhiza hairy root; analyzing the expression of AtMYC2 in the transgenetic salvia miltiorrhiza hairy root and related genes in biosynthetic pathways of tanshinone and salvianolic acid through qRT-PCR; measuring the contents of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a high-performance liquid chromatography (HPLC); and measuring the antioxidant activity of tanshinone and salvianolic acid in the transgenetic salvia miltiorrhiza hairy root by using a DPPH free radical scavenging method. The invention provides the method for simultaneously increasing the contents of tanshinone and salvianolic acid in salvia miltiorrhiza hairy root and also provides a novel high-quality raw material for producing tanshinone and salvianolic acid with important clinic demands so as to have the positive promoting significance and application value for relieving the problem that the drug resources of tanshinone and salvianolic acid are short.

The invention discloses an identification method for rape honey doped in acacia honey. Firstly, chemical compounds of phenolic acids are extracted from honey by means of resin, and chromatographically pure methyl alcohol of a metered volume is utilized to prepare a test solution; then certain amounts of ten kinds of phenolic acid comparison products are respectively taken, and chromatographically pure methyl alcohol of a metered volume is utilized to prepare a comparison product solution. The determination method comprises the steps that the comparison product solution and the test solution are respectively absorbed to be injected into a high performance liquid chromatograph, a chromatogram is measured and recorded by making the comparison products as reference substances, an HPLC-ECD fingerprint spectrum of the acacia honey is obtained, an HPLC-ECD fingerprint spectrum of the rape honey and an HPLC-ECD fingerprint spectrum of the acacia honey containing the rape honey are obtained by means of the same method, the three spectrums are compared, and by combining a chemometrics method, whether the rape honey is doped in the acacia honey is identified. The identification method is reasonable in design, good in separation effect and high in sensitivity and feasibility, and resolves an important technical problem in acacia honey adulteration identification and quality control.