6282 results about "Spore" patented technology

An analyte is separated from a fluid sample by introducing the sample into a cartridge having an extraction chamber containing capture material for capturing the analyte. The sample is forced to flow through the extraction chamber to capture the analyte with the capture material in the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber. The cartridge may optionally include a lysing region for lysing sample components (e.g., cells spores, or microorganisms), a waste chamber for storing waste fluid, and reaction or detection chambers for chemically reacting or detecting the eluted analyte.

Methods are disclosed for sterilizing tissue to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria, (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, spores, prions or similar agents responsible, alone or in combination, for TSEs and / or single or multicellular parasites. The methods involve sterilizing one or more tissues with irradiation.

A device for use with an ultrasonic transducer to lyse components of a fluid sample comprises a cartridge having a lysing chamber, an inlet port in fluid communication with the lysing chamber, and an outlet port for exit of the sample from the lysing chamber. The inlet and outlet ports are positioned to permit flow of the sample through the lysing chamber, and the chamber contains at least one solid phase for capturing the sample components to be lysed as the sample flows through the chamber. The lysing chamber is defined by at least one wall having an external surface for contacting the transducer to effect the transfer of ultrasonic energy to the chamber.

A device for lysing components (e.g., cells, spores, or microorganisms) of a fluid sample comprises a cartridge having a lysing chamber for receiving the sample and having at least one solid phase in the lysing chamber for capturing the sample components to be lysed. An ultrasonic transducer is coupled to a wall of the lysing chamber to transfer ultrasonic energy to the captured sample components.

Methods of beverage and flowable food product production using steam injection are provided to efficiently destroy microorganisms able to withstand normal pasteurization temperatures. Microorganisms such as Alicyclobacillus acidoterrestris and its spores may be eliminated from fruit juices and the like while minimizing organoleptic degradation due to heating. The apparatus is capable of pasteurizing, blending and controlling the product specifications of a finished beverage in a continuous matter.

Compositions including a non-pathogenic lactic acid-producing bacteria, such as a Bacillus species, spores or an extracellular product of B. coagulans, formulated for oral administration to the intestinal tract for inhibiting bacterial gastrointestinal infections are described. Methods and systems using the compositions for treating gastrointestinal infections, particularly sudden infant death syndrome (SIDS) are also disclosed.

A method and apparatus is described for neutralizing airborne pathogens and chemical toxins in ventilated air, and in heating or air conditioning systems. The pathogen-chemical toxin neutralization system is effective against a wide spectrum of pathogens and toxins, it incorporates commercially available components, and it can be readily integrated into commercial HVAC systems where it decontaminates large volumes of ventilated air in real time without any chemical reagents. The system has a flow-through reaction chamber (101) that contains a UV light source (106) that emits short intense flashes of broad-spectrum UV light, a source aqueous hydrogen peroxide that can be a reservoir or a hydrogen peroxide generator (106), and optionally a source of ozone. The interaction of UV light and hydrogen peroxide generates hydroxyl radicals that neutralize pathogens and chemical toxins as they pass through the reaction chamber (101) in real time. The pathogens that can be neutralized by this system include bacteria, viruses, spores, fungi and parasites.

The present invention discloses compositions derived from an isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain, suitable for topical application to the skin or mucosal membranes of a mammal, which are utilized to inhibit the growth of bacterium, yeast, fungi, virus, and combinations thereof. The present invention also discloses methods of treatment and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, by topical application of therapeutic compositions which are comprised, in part, of isolated Bacillus species, spores, or an extracellular product of Bacillus coagulans comprising a supernatant or filtrate of a culture of said Bacillus coagulans strain. In addition, the present invention also discloses compositions, methods of treatment, and therapeutic systems for inhibiting the growth of bacterium, yeast, fungi, virus, and combinations thereof, comprising an extracellular product of Pseudomonas lindbergii comprising a supernatant or filtrate of a culture of said Pseudomonas lindbergii strain.

The present invention is directed to a method for the disinfection and sterilization of material and surfaces contaminated with one or more members selected from the group consisting of bacteria and bacterial spores, comprising the steps of: (a) providing a biocidal fluid containing a mixture of effective amounts of a germinant and a germicide; and (b) contacting the material and surfaces contaminated with one or more members selected from the group consisting of bacteria and bacterial spores, with the biocidal fluid of step (a) for a time sufficient for disinfecting and sterilizing said material. The invention also provides a sterilizing composition suitable for killing and rendering spores lifeless comprising: (a) an effective amount of a germinating agent; (b) an effective amount of a germicide.

A substrate comprising a crosslinked polymer primer layer, and grafted thereto a ligand-functionalized polymer is provided. The grafted polymer has the requisite affinity for binding neutral or negatively charged biomaterials, such as cells, cell debris, bacteria, spores, viruses, nucleic acids, and proteins, at pH's near or below the pI's of the biomaterials.

The present invention relates to shelf stable and / or less corrosive peroxycarboxylic acid antimicrobial compositions, including ready-to-use compositions. Shelf stable compositions can include defined ratios of hydrogen peroxide to peroxycarboxylic acid and / or hydrogen peroxide to protonated carboxylic acid, but need not include strong acid. Reduced corrosion compositions can include carboxylic acid and corrosion inhibitor at acid pH. Compositions of the invention can have advantageous activity against spores.

Sterilization methods and apparatus are effective to achieve a 6-log reduction in CFUs of industry standard bacteria and bacterial spores, i.e., B. stearothermophilus and B. subtilis spores, by subjecting sterilizable materials to a chemical additive-containing carbon dioxide sterilant fluid at or near its supercritical pressure and temperature conditions. Most preferably, the chemical additive-containing supercritical carbon dioxide sterilant fluid is agitated during sterilization, e.g., via mechanical agitation or via pressure cycling.

A method for preparing a sample suspected to contain a target nucleic acid sequence for a nucleic acid amplification reaction and for verifying the effectiveness of the sample preparation comprises the step of mixing the sample with sample preparation controls. The sample preparation controls are cells, spores, microorganisms, or viruses that contain a marker nucleic acid sequence. The sample mixed with the sample preparation controls is subjected to a lysis treatment, and nucleic acid released by the lysis treatment is subjected to nucleic acid amplification conditions. The presence or absence of the target nucleic acid sequence and of the marker nucleic acid sequence is then determined. Positive detection of the marker nucleic acid sequence indicates that the sample preparation process was satisfactory, while the inability to detect the marker nucleic acid sequence indicates inadequate sample preparation.

This invention relates to a method, process and apparatus for disinfecting and sterilizing all types of surfaces contaminated with microorganisms and toxic substances to render both inactive. Furthermore, this invention relates to both a method and apparatus for disinfecting and / or sterilizing breathable air and then using this air to protect a confined space from external contamination. The apparatus consists of a new ultra-violet (NUV) source that is more effective than mercury based 254 nm light for destroying DNA of virus, bacteria, spores and cists. It is most effective in breaking chemical bonds in toxic gases and Biotoxins that are useful to terrorists. It is combined with other apparatus that remove particulates and byproducts sometimes produced by the NUV source and maintains positive pressure of the confined space so as to prevent the influx of air from outside the protected zone.

The subject invention provides novel and efficacious systems and methods for particle, chemical, and / or biocompound sensing. In one embodiment, the system of the invention comprises a sensing device that includes a membrane containing at least one nanochannel that spans all or substantially all of the thickness of the membrane. The nanochannel(s) of the invention can be functionalized to enhance target analyte detection and quantification. In one embodiment, the nanochannel is conically shaped and includes a molecular recognition agent for a target analyte. In certain operations, the sensing systems of the invention quantitatively and qualitatively detect biochemical / biomedical species and biomacromolecules, such as proteins, DNA, cells, spores and viruses, with a high degree of sensitivity and specificity.

A pesticidally active isolate of the fungus Beauveria bassiana, which is monosporic and which may be used in agriculture as a pesticide for the effective control of pests. The biomass of the inventive isolate contains an unusually high proportion of spores. The invention also provides methods for the preparation of the isolate and methods of formulating and using same for the effective control of crop infesting pests.

Current chemotherapeutic approaches for cancer are in part limited by the inability of drugs to destroy neoplastic cells within poorly vascularized compartments of tumors. We have here systematically assessed anaerobic bacteria for their capacity to grow expansively within avascutar compartments of transplanted tumors. Among 26 different strains tested, one (Clostridium novyi) appeared particularly promising. We created a strain of C. novyi devoid of its lethal toxin (C. novyi-NT) and showed that intravenously injected C. novyi-NT spores germinated within the avascular regions of tumors in mice and destroyed surrounding viable tumor cells. When C. novyi-NT spores were administered together with conventional chemotherapeutic drugs, extensive hemorrhagic necrosis of tumors often developed within 24 hours, resulting in significant and prolonged anti-tumor effects. This strategy, called combination bacteriolytic therapy (COBALT), has the potential to add a valuablle dimension to the treatment of cancer.

A biosensor system is provided for detecting biological species such as bacteria, proteins, viruses, spores and DNA or RNA. The biosensor system may also be able to distinguish between Gram positive and Gram negative bacteria. In some embodiments, the analyte solution flows past a detector surface to a filter, which traps the analyte. The analyte is then washed back past the detector surface, thus increasing the number of analyte species that become attached to the surface. The detector may include an optical microresonator that is optically coupled via waveguides to a light source and an optical detector. One of the waveguides may be provided with a wavelength selective reflector to increase the amount of probe light coupled into the microresonator or to increase the fraction of the signal light detected by the optical detector.

The current invention relates to the field of detection and identification of clinically important fungi. More particularely, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cyptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.

The present invention provides a composition, comprising: greater than about 0.1% by weight hydrogen peroxide; an aromatic acid component; surfactant; optionally, a solvent; and a carrier. The composition of the invention is useful as a disinfecting composition for killing microorganisms such as bacterium (including Mycobacterium), spores and fungi. The composition provides a pathogenic bacteria kill rate of 99.9% in about 30 seconds when bacteria are exposed to the composition and is effective in providing a Mycobacterium kill of 106 with two minutes or less. Moreover, the compositions of the invention are generally more resistant to catalase deactivation than, for example, an aqueous solution of hydrogen peroxide. The concentration of hydrogen peroxide within the composition may range from about 1% by weight to about 7% by weight and the concentration of aromatic acid component may range from about 0.1% by weight to about 5% by weight. The invention also provides a method for disinfection of a substrate utilizing the composition. The composition of the invention may be used in the foregoing method on a medical instrument, such as an endoscope or the like. Applying the compositions to a substrate may be accomplished in any of a variety of application methods such as by roll coating, dipping, spraying, or rotational tumbling. The composition may be applied to the substrate for a period of time ranging from about 30 seconds to about ten minutes. In this aspect, the invention can further comprise drying the substrate after removing the composition.

A swab comprising gelatine or collagen has been found to have a remarkably high recovery of microorganisms. Furthermore, the samples, such as microorganisms, spores, nucleotides and other biologically or biochemically relevant compounds can be fully recovered from the collagen- or gelatine-comprising swab. The invention thus provides a method and swab which has a high recovery of a target from a sample and furthermore a second high recovery when transferring from the swab to a medium for analysis.

The invention relates to a method for producing parental lines for a hybrid organism. This method is based on the segregation of individual alleles in the spores produced by a desired plant and / or in the progeny derived from the self-pollination of that desired plant, and on the subsequent identification of suitable progeny plants in one generation, or in a limited number of inbred cycles. The invention further relates to a method for reconstructing a partially heterozygous starting organism.

Germicidal compositions with enhanced activity towards killing microbiological spores and vetgetative cells comprising certain quaternary ammonium compounds (QACs), phenolic compounds, monohydric alcohols, hydrogen peroxide, iodine, triclocarban, triclosan or combinations thereof with one or more spore coat opening agents. The invention also provides for the application of the germicidal compositions to animate and inanimate surfaces to help kill germs and protect against the risk of infection from bacteria, molds, yeasts, fungi, viruses, and microbiological spores.

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

The invention discloses a composite type artificial algal reef comprising a mature algal reef and a seedling reef. The mature algal reef comprises a solid base. The upper surface of the solid base is embedded with a metal frame, wherein the shape of the metal frame is identical to that of the upper surface of the solid base and the size of the metal frame is identical to that of the upper surface of the solid base. A conch net bag is arranged in the metal frame. The center of the upper surface is embedded with a metal stand column. A steel ring is welded to the upper end of the stand column. A plurality of seedling ropes are fixed between the metal frame and the steel ring. The seedling reef is fixed to the side face of the solid base of the mature algal reef, and algal seedlings are attached to the seedling reef. By the adoption of the composite type artificial algal reef, the attaching effect of alga is guaranteed, the repair period of an algae field is shortened, the success rate of repair and reconstruction of the algae field is improved, the adhesive rate of the seedlings is increased, and the seedling reef is firm, durable, small in size, light, and convenient to carry; the net bag made of waste conches is placed in the metal frame, and a biophile reef can be composed through diffusion and attachment of spores of fronds on the seedling ropes; resource utilization of the waste conches is achieved, and composite type artificial algal reef has the advantages of being economical and easy and convenient to use.

The invention discloses microbial straw decomposition additive, which belongs to the field of straw decomposition additive and is obtained through combining four bacteria: bacillus subtilis, aspergillus niger, trichoderma reesei and saccharomyces cerevisiae. The microbial straw decomposition additive utilizes the temperature rise benefit of the aspergillus niger spore enzyme activity for raising the environment temperature of decomposition, the further temperature rise benefit in the growth process of the bacillus subtilis is cooperated, and the temperature required by the decomposition is ensured, so the decomposition effect of straws is improved. The microbial straw decomposition additive has the obvious temperature rise benefit and can be used for the in-site field returning decomposition of the straws, the straw decomposition can be better accelerated in low-temperature and normal-temperature environment, the bottleneck problem of straw utilization is solved, the straw burning can be effectively reduced, the decomposition work procedure and the labor cost are saved, the fertilizer feeding is reduced, the soil structure is improved, and the planting benefit is improved.

Compositions, methods and business applications of using new and recycled cardboard infused with a plurality of saprophytic (including endophytic) and mycorrhizal fungi matched with seeds of plants (including trees, vegetables, herbs and grasses) whereby the cardboard can be sprouted by end-users to start ecosystems. Such containers may have carbon-credit value for companies and consumers when planted and grown as a carbon sink or carbon offset for the photosynthetic and mycelial sequestration of carbon dioxide. The relative weight of the Life Box's added seeds and spores does not significantly affect the total weight of the infused cardboard, thus not increasing transportation costs.

The invention relates to enterococcus faecium for feeding as well as a freeze-drying fungicide and applications thereof, and discloses enterococcus faecium LAB12 CGMCC (China General Microbiological Culture Collection Center) No.4847 which is grampositive cocci, has no spores, grows well on an MRS agar plate, forms a round bacterial colony with the diameter of 0.5-1mm within 48 hours and is used for feeding, and the bacterial colony is round, smooth and upheaved, and is a shape of grey white dewdrop; the enterococcus faecium grows in a facultative anaerobic condition; the growth temperature range is 10 DEG C-45 DEG C; the optimum growth temperature is 30 DEG C-40 DEG C; and the growth pH value is 4-10, and the optimum pH value is 6.0. The freeze-drying fungicide formed by the bacterial strain is nontoxic and harmless, is gastric juice resistant, is cholate resistant, has a high inhibitory effect for multiple harmful bacteria, has a long quality guarantee period, can be widely applied to birds and livestock breeding to strengthen the animal disease-resistant capability, and is expected to be the substitution of antibiotics for feeding.