31 results about "Ustilago" patented technology

The invention relates to the field of pesticide preparations, disclosed a suspension seed coating agent which comprises the active components chlorantraniliprole and thiram and has the effects of killing insects and preventing diseases. The seed coating agent is characterized by being composed of the following compositions in percent by weight: 3.0-20.0% of chlorantraniliprole, 4.0-12.0% of thiram, 1.0-4.0% of tebuconazole, 2.0-4.5% of a wetting dispersant (prepared by compounding pesticide emulsifier 34 and HY-2), 0.1-0.5% of a thickening agent, 0.1-4.0% of a film forming agent, 3.0-5.0% of an antifreezing agent, 0.1-3.0% of an aposematic agent, 0.1-1.0% of an antifoaming agent and the balance water. The seed coating agent provided by the invention has the characteristics of being convenient to prepare, uniform in coating, low in shedding rate, high in efficiency, low in toxicity, relatively safe to environment and the like, and has substantial control effect on corn seedling-stage insect diseases such as sphacelotheca reiliana, ustilago maydis, stem rot, cutworms, athetis lepigone, grub and the like, the control effect on corn seedling-stage insect diseases reaches 90% or more, and the persistent period reaches about 2 months.

The present invention relates to a maize mushroom production method, and belongs to the technical field of mushroom cultivation. The method comprises: culturing ustilago maydis to prepare an inoculum solution with suitable concentration; after the susceptible maize hybrid tasseled, injecting the inoculum solution with suitable concentration through the filament passage into the top of corn cobs; and harvesting the maize mushroom in an adaptive period after inoculation. According to the maize mushroom produced by using the method provided by the present invention, the method has the advantages that the operation is simple, the yield is high, the appearance quality is good, and the batch production can be realized, and the method is a good method worth being promoted and applied.

The invention belongs to the field of agricultural fungicide and particularly relates to an agricultural compound fungicide. The effective components of the fungicide are pyraclostrobin and fenpyrazamine which are compounded. The weight ratio of the two components is 1: 0.1-10, preferably, 1: 2-3. The prepared fungicide expands fungicide spectrum and has high activity on botrytis cinerea, rhizoctonia, puccinia, ustilago, tilletia, corticium, pyrenophora and other pathogenic fungi, and is remarkable in synergy which is not simple composition of activity of the components. The fungicide is strong in preventive treatment efficacy, long in effect lasting time, small in resistance risk and capable of reducing field usage of effective components, reducing production and using cost and reducing fungicide residue and environment pollution. The fungicide is favorable for overcoming and delaying production of bacteria drug resistance and is safe for crops due to effective components with different action mechanisms.

The invention provides a rapid detection method for millet smut fungi and belongs to the technical field of biological detection. Whether smut fungi exists in a sample or not is detected through extracting millet smut fungi spore genome DNA (Deoxyribonucleic Acid), designing a PCR (Polymerase Chain Reaction) specific primer according to the difference between a millet smut fungi pathogenic bacteria ITS sequence and an ITS sequence of other ustilago on NCBI, carrying out PCR amplification by applying the designed specific primer of the millet smut fungi, carrying out electrophoresis on an amplification product with 1 percent agarose gel, photographing with a gel imaging system and comparing with a standard molecular weight. The detection verifies that a detection system provided by the invention has the advantages of simplicity in operation, rapidness and accurate detection result.

The invention discloses new applicationfor fungicidal synergism of farnesol and methoxy acrylic fungicides to prevention and treatment of smut disease. The synergistic combination can be applied to prevention and treatment of sugarcane, corn and other crops caused by sporosorium and ustilago, has a high synergistic effect, and can significantly reduce the use of chemical pesticides, and the prevention and treatment effect is significantly better than that of a single agent.

The invention discloses new applicationfor fungicidal synergism of farnesol and triazole fungicides in prevention and treatment of smut of sugarcane, corn and other cropscaused by sporisorium and ustilago. Farnesol and triazole fungicides have obvious synergistic effects,can greatly reduce the use of chemical pesticides, have the prevention and treatment effect obviously superior to that of a single agent, and thus have a good application prospect.

This invention encompasses the identification and isolation of genes that confer disease control properties in plants, as well as plants comprising such genes. These genes are derived from the following sources: Nicotiana benthamiana, Oryzae sativa (var. Indica IR7), Papaver rhoeas, Saccharomyces cerevisiae and Trichoderma harzianum (Rifai 1295-22). The control conferred is against the one or more of the following phytopathogens: Aspergillus flavus, Cercospora zeae-maydis, Fusarium monilforme, Fusarium graminearum, Helminthosporium maydis, Phoma lingam, Phomopsis helianthi, Phytopthera infestans, Pyricularia oryzae, Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum, Ustilago maydis, and Verticillium dahliae. Further, this invention encompasses other homologous and heterologous sequences with a high degree of functional similarity.

The invention discloses an avermectin derivative, which is extracted and separated from Anthogorgia caerulea, and is named as 5-O-dimethyl-25-bis(1-methylpropyl)-25-(1-methylbutyl)avermectin Ala. Tests indicate that the EC50 value of the avermectin derivative Blc on ustilago scitaminea MAT-1 and MAT-2 matching basidiospore proliferation is far less than a positive control azoxystrobin suspending agent, and has good avermectin derivative inhibition activity. The compound Blc is a marine natural active component, also is easily soluble in methanol and ethanol, and has good alcohol affinity, thus being applicable individually or in combination to preparation of efficient biopesticides for control of sugarcane ustilago. The invention provides a new use for development of avermectin derivatives, and provides a new source for preparation of novel pesticides inhibiting ustilago scitaminea.

A spinach and kiwifruit juice is prepared from following raw materials including, by weight, 300-350 parts of kiwifruits, 100-120 parts of fresh oranges, 40-50 parts of oat, 40-50 parts of cucumbers, 20-30 parts of carrots, 20-25 parts of spinaches, 30-40 parts of brown sugar juice, 20-30 parts of fresh ginger juice and the like. The invention discloses the spinach and kiwifruit juice and a preparation technology thereof. The preparation technology is simple. The spinach and kiwifruit juice is prepared with addition of the oat, the carrots and the spinaches so that the spinach and kiwifruit juice is thick in fragrance and is rich in nutrition. By means of additions of extracts of semen cassiae, gynostemma pentaphyllum, radix rehmanniae preparata, ustilago maydis and the like, the spinach and kiwifruit juice has a certain blood fat reducing effect, is suitable for people whom is suffered from hyperlipidemia and obesity, and is beneficial to body health.

The invention discloses health food and in particular relates to nutritious nano casing convenience food containing ustilago sorghum. The nano casing convenience food is prepared by the following raw materials in parts by weight: 4-8 parts of nano ustilago sorghum powder, 1-4 parts of nano pearl barley powder, 9-11 parts of nano sorghum rice powder, 1-4 parts of nano millet powder and nano black rice powder. The food is hermetically packed with casing, can be stored for a long time, dispenses with preservatives, is good and smooth in mouthfeel, convenient to eat and rich in nutrition, and can be eaten after the bag is opened or after being heated or as a cold dish. Ustilago sorghum is added in the food, which has anti-aging effect on the human bodies, has strong restraint against tumor cells and can promote synthesis of proteins and nucleic acid, and the nutritional components of ustilago sorghum are well preserved in the food, therefore the food also has anti-aging and anti-tumor effects through test verification.

The present invention relates to a maize mushroom production method, and belongs to the technical field of mushroom cultivation. The method comprises: culturing ustilago maydis to prepare an inoculum solution with suitable concentration; after the susceptible maize hybrid tasseled, injecting the inoculum solution with suitable concentration through the filament passage into the top of corn cobs; and harvesting the maize mushroom in an adaptive period after inoculation. According to the maize mushroom produced by using the method provided by the present invention, the method has the advantages that the operation is simple, the yield is high, the appearance quality is good, and the batch production can be realized, and the method is a good method worth being promoted and applied.

The present invention relates to the application of isolated promoters and synthetic dominant selection constructs and enhancers for gene targeting for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the Rhodosporidium, Sporisorium, Sporobolomyces or Ustilago genera.

The present invention discloses a feed additive for improving hair quality and hair color of cats and a preparation method thereof. The feed additive consists of the following raw materials: fructus alpiniae oxyphyllae, metaplexis japonica, radix polygonati officinalis, dandelions, saussurea involucrata flowers, sedum aizoon, siberian nitraria fruits, white perilla stems, ashycoloured ironweed herb, lucid ganoderma, melodinus suaveolens fruits and rhizoma curcumae; and preferably, further comprises the following raw materials: ovateleaf holly bark, cassia seeds, ustilago nuda, Chinese flowering quince fruits and stems or leaves of fortune euonymus. The beneficial effects are as follows: the feed additive can comprehensively improve the health of cats' skin-hair, protect hair follicle and hair from damages, enable hair to be bright and smooth, reduce hair loss, promote regeneration of skin-hair, enhance immunity of the cats, and enable the cats' skin-hair to be smooth and bright, bright in color, and smooth and flexible, and can increase hair quality and brighten hair color. The raw materials are all natural products, so that the feed additive has advantages of being high in security, free of drug residues, toxic or side effects, etc.

The invention discloses a recombinant vector for expressing an ustilago maydis effector protein CMU1 gene and further discloses a method for expressing the ustilago maydis effector protein CMU1 gene.A prokaryotic expression vector of the ustilago maydis effector protein CMU1 gene is constructed, a series of optimization is performed on protein expression conditions, CMU1 soluble protein is finally obtained, and the result lays a foundation for further study on crystal structures and biological characteristics of protein, provides a thought for designing and developing pesticides targeting CMU1 and further provides a basis for control of ustilago maydis.

The invention discloses a fungicide composition containing metalaxyl and thifluzamide. The fungicide composition comprises the effective components, including metalaxyl and thifluzamide which are compounded, and the balance of auxiliary components, wherein the mass ratio of the effective components metalaxyl and thifluzamide in the fungicide composition is (1-50) to (50-1), and the mass part ratio of the effective components metalaxyl and thifluzamide in the preparation is 1%-80%, and the balance is auxiliary components required to be used and accepted in pesticides. The fungicide composition has the dosage forms of missible oil, suspension, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules and water aqua, is mainly used for preventing and controlling powdery mildew of wheat, strawberries, grapes, apples, and cucumbers, meanwhile can effectively prevent and control fungi such as rhizoctonia, puccinia, ustilago, tilletia, corticium, pyrenophora, and especially has special effects to banded sclerotial blight, damping off and the like due to basidiomycotina fungus.

The invention discloses a Ustilago-maydis haploid strain UM02. The collection number of the Ustilago-maydis haploid strain UM02 is CGMCC No.15387. The Ustilago-maydis haploid strain UM02 is combined with a haploid strain matched with the Ustilago-maydis haploid strain UM02 in mating type, then the pathogenicity is high, the success rate of inoculation is high, an identification result of the ustilago maydis resistance of maize varieties is accurate and reliable, and the consistency is good. When the Ustilago-maydis haploid strain UM02 is used for maize mushroom production, the yield of maize mushrooms is high, husk leaves are wrapped with the Ustilago-maydis haploid strain UM02, and harvesting and transportation are convenient.

The invention discloses an Ustilago maydis haploid strain UM01. The Ustilago maydis haploid strain UM01 has a preservation number of CGMCC No. 15263. Through combination of the Ustilago maydis haploidstrain UM01 and a haploid strain matching with the mating type, the pathogenicity is strong and a success rate of inoculation is high. The Ustilago maydis haploid strain UM01 produces accurate and reliable identification results of resistance to Ustilago maydis-caused diseases and has good consistency. In corn mushroom production, a corn mushroom yield is high and the corn mushrooms are easy to collect and transport.

The invention relates to a bactericidal composition containing cyflufenamid and thifluzamide. The bactericidal composition comprises active ingredients including the cyflufenamid and the thifluzamide in a binary compound way, and the balance of auxiliary components, wherein the mass ratio of the active ingredient cyflufenamid to the active ingredient thifluzamide in the pesticide composition is (1-50): (50-1); the preparation comprises the active ingredients including the cyflufenamid and the thifluzamide with the mass percent of 1-80%, and the balance of acceptable auxiliary components which are allowed to be used in pesticide. The pesticide composition can be prepared into different preparations such as missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules or aqueous solution, is mainly used for controlling the powdery mildew of wheat, strawberries, grapes, apples and cucumbers, is capable of effectively controlling fungi such as rhizoctonia, puccinia, ustilago, tilletia, corticium and pyrenophora, and especially has special effect for diseases such as banded sclerotial blight and damping off caused by basidiomycotina fungus.

The present invention relates to the application of isolated promoters and synthetic dominant selection constructs and enhancers for gene targeting for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the Rhodosporidium, Sporisorium, Sporobolomyces or Ustilago genera.

A spinach and kiwifruit juice is prepared from following raw materials including, by weight, 300-350 parts of kiwifruits, 100-120 parts of fresh oranges, 40-50 parts of oat, 40-50 parts of cucumbers, 20-30 parts of carrots, 20-25 parts of spinaches, 30-40 parts of brown sugar juice, 20-30 parts of fresh ginger juice and the like. The invention discloses the spinach and kiwifruit juice and a preparation technology thereof. The preparation technology is simple. The spinach and kiwifruit juice is prepared with addition of the oat, the carrots and the spinaches so that the spinach and kiwifruit juice is thick in fragrance and is rich in nutrition. By means of additions of extracts of semen cassiae, gynostemma pentaphyllum, radix rehmanniae preparata, ustilago maydis and the like, the spinach and kiwifruit juice has a certain blood fat reducing effect, is suitable for people whom is suffered from hyperlipidemia and obesity, and is beneficial to body health.

The invention relates to a haploid strain of Ustilago smut UeTSP and an application thereof, belonging to the field of biotechnology. On the one hand, the present invention provides a new Ustilago smut haploid strain UeTSP, and on the other hand provides the use of the Ustilago smut haploid strain UeTSP in pregnant bamboo. Beneficial effects of the present invention: the smut fungus of the present invention ( Ustilago esculenta ) The haploid strain UeTSP can directly infect Zizania japonica plants and make Zizania zizania gestation without producing teliospores, that is, without the unfavorable phenotype of gray horns, which provides a new idea for zizania zizania artificial pregnancy.

In Magnaporthe species and other plant pathogenic fungal species, the appressoriu, (infection structure) is responsible for breaching the host plant cell wall and gaining entry into the host tissues. Magnaporthe ABC3 protein is an MDR transporter that plays an important role during host penetration and also is involved in regulating fungal response to intracellular oxidative stress. The insertional mutant abc3Δ, in which the ABC3 efflux pump function is blocked, lacks functional appressoria and is therefore incapable of causing disease in host plants. This invention provides the abc3 nucleic acid (gene) and ABC3 protein from Magnaporthe or from Aspergillus, Ustilago or Fusarium and describes methods for reducing plant pathogenicity for important rice pathogens.

The invention discloses a formula of a traditional Chinese medicine for treating hypotension and a preparation of the traditional Chinese medicine. The traditional Chinese medicine is prepared from the following raw materials by weight: 5g of spatholobus sinensis, 3g of pungent litse branchlet, 3g of talinum crassifolium, 5g of ustilago maydis, 6g of processed fleece-flower root, 3g of manyinflorescenced sweetvetch root, 5g of oreas, 6g of tofieldia yunnanensis franch, 6g of ginkgo root, 3g of areneria kensuensis, 5g of mulberry fruit, 3g of polygala fallax hemsl, 10g of panax ginseng, 6g of eleutherine plicata herb and 5g of eleutherococcus brachypus. The traditional Chinese medicine for treating hypotension is convenient to use, does not have side effect, has low price and drug effect, and cannot cause potential hazard when taken.

The invention relates to liver-protecting digestion tea and a preparation method thereof and belongs to the technical field of health-care tea. The liver-protecting digestion tea is prepared by processing honeysuckle, ustilago maydis and aloe. When the liver-protecting digestion tea is prepared, the ustilago maydis and aloe leaves are sliced and are mixed with dry honeysuckles; the dry honeysuckles are capable of absorbing ustilago maydis slices and soluble substances in an aloe vera gel; and meanwhile, ustilago maydis spores are in full contact with the honeysuckle through adhesion of the aloe vera gel, thereby reducing the dispersion and loss of the spores in a drying process, and preventing active substances from losing. After the ustilago maydis spores and the honeysuckle are mixed and fried, volatiles of the mixture blend mutually and promote mutually. When the liver-protecting digestion tea is soaked, the active substances are easy to leach out, and the taste is further improved. As the liver-protecting digestion tea is prepared by taking the honeysuckle, the ustilago maydis and the aloe as raw materials, a novel variety of health-care tea is increased, wastes are effectively utilized; and the three raw materials are reasonably matched and made, so that pharmacodynamic effects of the honeysuckle and the aloe are gentle, thus the liver-protecting digestion tea is convenient to drink by most people.

The invention relates to the field of pesticide preparations, disclosed a suspension seed coating agent which comprises the active components chlorantraniliprole and thiram and has the effects of killing insects and preventing diseases. The seed coating agent is characterized by being composed of the following compositions in percent by weight: 3.0-20.0% of chlorantraniliprole, 4.0-12.0% of thiram, 1.0-4.0% of tebuconazole, 2.0-4.5% of a wetting dispersant (prepared by compounding pesticide emulsifier 34 and HY-2), 0.1-0.5% of a thickening agent, 0.1-4.0% of a film forming agent, 3.0-5.0% of an antifreezing agent, 0.1-3.0% of an aposematic agent, 0.1-1.0% of an antifoaming agent and the balance water. The seed coating agent provided by the invention has the characteristics of being convenient to prepare, uniform in coating, low in shedding rate, high in efficiency, low in toxicity, relatively safe to environment and the like, and has substantial control effect on corn seedling-stage insect diseases such as sphacelotheca reiliana, ustilago maydis, stem rot, cutworms, athetis lepigone, grub and the like, the control effect on corn seedling-stage insect diseases reaches 90% or more, and the persistent period reaches about 2 months.

The invention relates to a sterilizing composition containing sedaxane and fluopicolide. The active component of the sterilizing composition is formed by compounding the sedaxane and the fluopicolide,and the rest is auxiliary components. The mass ratio of the active components, namely the sedaxane to the fluopicolide, of the pesticide composition is (1-50):(50-1), the mass part ratio of the active components, namely the sedaxane to the fluopicolide, in the preparation is 1 percent to 80 percent, and the rest is auxiliary components which are allowed to use and accept in the pesticide. The dosage forms of the pesticide composition are missible oil, a suspending agent, and the pesticide composition is mainly used for preventing and treating powdery mildew on triticeae, strawberries, grapes,apples and cucumbers; and moreover, the pesticide composition can effectively prevent and control fungi such as rhizoctonia, puccinia person, ustilago, tilletia, corticium and pyrenophora, in particular has a special effect on diseases, such as rhizoctonia solani and damping off, caused by basidiomycetes.

The invention discloses a bactericidal composition, which is prepared from triticonazole and fludioxonil as effective components, and the mass ratio of triticonazole to fludioxonil is (50:1)-(1:50). The composition disclosed by the invention has obvious synergistic effects, is effective to diseases caused by fusarium, ustilago, tilletia, pyrenophora teres, septoria, rhizoctonia solani and the like, is mainly used for preventing and treating diseases of cereal crops, leguminous crops and fruit trees, has special effects on seed-borne diseases, has a lasting period of 4-6 weeks, and can be usedfor preventing and treating diseases of cereal crops and especially preventing and treating ustilago tritici, stinking smut, wheat stem rot, maize head smut, maize stem rot, maize gall smut and rice bakanae disease in seed treatment.

The invention discloses traditional Chinese and western medicine compound injection for great occipital neuralgia and a preparation method thereof. The injection is prepared from the following medicinal raw materials: toona leaves, Indian nightshade roots, loquat cores, iris confusa, neocinnamomum lecomtei liou, ustilago nuda, urtica macrorrhiza, alpine cladonia, delonix regia, calastrus flagellaris rupr, heterohairy monkshood root, root of scopolia sinensis hemsl, womwoodleaf ampelocissus root, hedyotis tenelliflora, clerodendron inerme, ternate-leaved rabdosia, pratt mountainash root-bark, haloragis micrantha, Japanese snake gourd root, osmanthus fragrans, dolomiaea berardioidea, wilson citrus seed, glomeris, atropine, vitamin C, thiamine, zinc gluconate and magnesium sulfate. The injection has the effects of activating blood circulation, dredging collaterals, dispelling wind and cold, diminishing swelling, removing stasis, soothing liver, regulating qi, diminishing inflammation and relieving pain, can be used for relieving local muscle spasm, improving local microcirculation of nerve involvement and nourishing nerves, and is advantageous to focus regeneration and repair. Furthermore, drugs can directly reach a focus with good absorption, by means of local injection, so that the injection has quick response, is convenient to treat, acupuncture, massage and other complicated processes can be reduced, and the blank of the prior art can be filled up.

The invention discloses an avermectin derivative, which is extracted and separated from Anthogorgia caerulea, and is named as 5-O-dimethyl-25-bis(1-methylpropyl)-25-(1-methylbutyl)avermectin Ala. Tests indicate that the EC50 value of the avermectin derivative Blc on ustilago scitaminea MAT-1 and MAT-2 matching basidiospore proliferation is far less than a positive control azoxystrobin suspending agent, and has good avermectin derivative inhibition activity. The compound Blc is a marine natural active component, also is easily soluble in methanol and ethanol, and has good alcohol affinity, thus being applicable individually or in combination to preparation of efficient biopesticides for control of sugarcane ustilago. The invention provides a new use for development of avermectin derivatives, and provides a new source for preparation of novel pesticides inhibiting ustilago scitaminea.