The invention belongs to the technical field of edible fungus breeding, and relates to a germination medium for basidiospores of Zizhi basidiospores and a germination method for basidiospores of Zizhi. Each 1000mL of the germination medium includes the following components: 160-240g of potatoes, 10-20g of glucose, 3 sucrose -8g, MgSO 4 0.8-1.5g, KH 2 PO 4 0.3-0.8g, 1.5-2g of agar powder, 150-200mL of Zizhi decoction, 40-80μL of glacial acetic acid, and distilled water as the balance. The invention provides the germination medium of the basidiospores of Zizhi lucidum by adding a specific content of glacial acetic acid to make glacial acetic acid and potatoes, glucose, sucrose, MgSO 4 、KH 2 PO 4 The combination of the agar powder and the boiled liquid of Zizhi increases the germination rate of basidiospores of Zizhi to 6.50%. The method for germination of basidiospores of Zizhi using the basidiospore germination medium of Zizhi is simple, easy to operate, and easy to popularize and apply on a large scale.