Lucid ganoderma basidiospore germination culture medium

Abstract

The invention discloses a lucid ganoderma basidiospore germination culture medium, and a method using the lucid ganoderma basidiospore germination culture medium to germinate lucid ganoderma basidiospore. The lucid ganoderma basidiospore germination culture medium is prepared from a lucid ganoderma decocting liquid, a PDA (potato dextrose agar) powder and water. The lucid ganoderma basidiospore germination method has the advantages that the germination rate of lucid ganoderma basidiospore is 10% or above, and is even up to 20%, so as to obtain lucid ganoderma spore monokaryontic strains; the problem of lower germination rate of lucid ganoderma basidiospore under the laboratory condition is solved, so as to lay a foundation for the monospore crossbreeding of lucid ganoderma.

Description

technical field [0001] The invention relates to the field of edible fungus breeding, in particular to a method for germination of ganoderma basidiospores. Background technique [0002] Ganoderma lucidum [Ganoderma lucidum (Curtis) P.Karst.] belongs to Basidiomycotina, Phyllomycetes, Phyllomycetes, Polyporaceae, and Ganoderma genus. "Shen Nong's Materia Medica" and "Compendium of Materia Medica" listed it as the top grade. It has a long history of application among the people, "benefits the heart, nourishes the middle, increases wisdom, does not forget", "long-term use makes light of the body and does not grow old, and prolongs life". . Modern pharmacological research also shows that Ganoderma lucidum has multiple effects: 1. Anti-tumor effect; 2. Immunomodulatory effect; 3. Anti-radiation effect; 4. Effect on nervous system; 7. The effect on the digestive system. The mating type of Ganoderma lucidum is quadrupolar heterothallic combination, that is, two different nuclei c...

AI Technical Summary

Problems solved by technology

However, the basidiospores of Ganoderma lucidum that have not germinated cannot produce primary hyphae, so it is impossible to breed Ganoderma lucidum varieties by means of hybridization, protoplast fusion or mutagenesis

Ganoderma lucidum spores have two layers of walls, which are very tough, and it is difficult to break them by general chemical and physical methods; it is very difficult to germinate in nature, and the germination rate is extremely low under laboratory conditions

Thereby limiting the selection and breeding of new varieties of Ganoderma lucidum

[0004] Although the use of protoplast monokaryonization technology can solve the problems caused by the extremely low germination rate of Ganoderma lucidum basidiospores and the difficulty in obtaining monokaryotic strains, the monokaryotic strains obtained through protoplast monokaryonization technology do not have meiosis, The resulting monokaryotic strains were not rich in genetic diversity

Therefore, it is difficult to obtain new strains with extensive variation through protoplast mononuclearization technology

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