The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) kit for detecting a human toxoplasma gondii antibody and a preparation method of the indirect ELISA kit, and belongs to the field of biotechnology. The kit comprises a recombinant antigen GRA15 pre-coated plate, a sample diluent, a positive control, a negative control, an enzyme conjugate, a blocking solution, a washingsolution, a color developing solution and a terminating solution, wherein the amino acid sequence of a recombinant antigen GRA15 is shown in SEQ ID NO: 1. Compared with the existing detection technology, the indirect ELISA detection method has the advantages of being simple to operate, good in repeatability, economic and suitable for large-scale sample detection. Toxoplasma gondii Chineses1 strain GRA15 protein isolated in China is used as the coating antigen of the indirect ELISA method, and the kit has higher specificity for detection of toxoplasma gondii infection of Chinese people.