46results about How to "Rich genetic diversity" patented technology

The invention discloses a method for ecologically restoring heavy metal contaminated soil. According to the method, heavy metals are absorbed by a plant growth regulator, an artificial chelating agent and bamboo willows in a combining manner. The method disclosed by the invention can be used for absorbing heavy metal ions, namely Pb, Cu, Zn, Cd and Cr, by utilizing the high-quality bamboo willows which is fast in growth, high in resistance and good in material and accelerating the absorption of the bamboo willows on the heavy metal ions by adopting the plant growth regulator and the artificial chelating agent, so that the purpose of ecological restoration for 3-4 years can be achieved; after the heavy metal contaminated soil is ecologically restored through the bamboo willows, the concentration of the Pb, the Cu, the Zn, the Cd and the Cr which are contained in the soil can accords to the II class standard of the 'Environmental quality standard for soils (GB15618-1995)'.

The invention discloses a litchi pollen collecting and storing method, which belongs to the technical field of fruit tree pollination. The method comprises the following steps: 1, collecting litchi anthers which are matured but not cracked at a developmental stage and putting the collected litchi anthers into a glass culture dish; 2, putting the glass culture dish in which the litchi anthers are placed into a drying box and drying the litchi anthers in the glass culture dish by using the drying box; 3, pouring the dried litchi anthers and the scattered pollen into a stainless steel screen, and putting the pollen into a centrifuge tube after enabling the pollen to pass through the stainless steel screen; and 4, sealing the pollen in the centrifuge tube and storing the pollen in an ultra-low temperature refrigerator at -86 DEG C. The method is simple, convenient and easy to implement, and can be used for performing a collecting operation even under different weather conditions. The obtained pollen is high in germination rate and can be stored for the longest time.

The invention provides a breeding method for a temperate corn employing tropical germplasm. The method comprises the steps that a temperate-tropical germplasm hybridized combination A*B or B*A crossed by a temperate germplasm selfing line A and a tropical germplasm selfing line B, a temperate-tropical germplasm hybrid C, a tropical germplasm hybridized combination D*E, or a tropical germplasm hybrid F serves as a breeding material; 1-2 generations are bred with a mixed pollination method or a method of mixed pollination after backcrossing; a single plant is selected for continuous selfing; a temperate-tropical germplasm selfing line X or a tropical germplasm selfing line Y having the characteristics of temperate germplasm and tropical germplasm is breeded through multi-generation pedigree selection; and then a temperate-tropical germplasm hybrid Z having the characteristics of the temperate germplasm and tropical germplasm is crossed. The breeding method has the benefits that the breeding time is short, the cost is low, the efficiency is high, and multiple tropical germplasm colonies not adapting to a temperate zone can be fully used.

The invention relates to a method for culturing a rice strain with the medium rice amylose content through the molecule label auxiliary selection. The method comprises the following steps: taking a K81 rice strain as a donor parent strain, taking a rice breed or strain with excellent agronomic characters and non-medium amylose content as a receptor parent strain, and carrying out the hybridization of the strains; after the hybridization, carrying out 2 to 3 times of backcross and then carrying out 3 to 5 times of inbreeding; and simultaneously planting the donor parent strain and the receptor parent strain for every generation. During the selection of the generations, a molecule label RA19 is used for auxiliary selection; a current generation individual strain containing a Wx gene of the donor parent strain is selected as the individual strain for the next backcross or inbreeding; and in addition, during the second selection and the later selection of inbred plant, the amylose content is determined for the selection. The method can be used to rapidly and effectively select the rice material with the medium amylose content, reduce the breeding scale and the breeding cost, improve the rice amylose content with high efficiency, and rapidly culture the rice strain with the medium rice amylose content.

The invention discloses a forage hedgerow soil conservation planting technology, comprising the following steps of: tillage and land preparation, seeding and seedling, and plantation. The technology has the following advantages that: the forage hedgerow can prominently reduce sediment loss amount and runoff and has good effect of fast controlling water and soil loss; the forage hedgerow promotes value-cost ratio of energy of the entire system and becomes an effective measure to enhance the agricultural efficiency in hilly areas; bean forage hedgerow can offer excellent shelter to ground spider so as to enrich the diversity of spiders in farmland. The technology not only focuses on ecological and social benefits, but can also create economical benefit, in addition, the technology realizes sustainable agricultural development on dry hillside fields, effectually controls water and soil loss of dry hillside farmlands, enriches soil fertility, enhances grain yield and can also produce a certain amount of high-grade forage to further guarantee the supply of partial feed for the development of animal husbandry in agricultural regions.

The invention discloses a rice male sterile protein and a coding gene and application thereof. The protein is one of 1) a protein formed by amino acid residue sequences of the SEQ ID No.2 in a sequence table or 2) a protein which is formed after one or more amino acid residues in the amino acid residue sequences of the SEQ ID No.2 in the sequence table are substituted and / or deleted and / or added and has the same activities as the amino acid residue sequences of the SEQ ID No.2. The rice male sterile protein gene can be used for breeding male sterile rice, and makes contributions for removing male hybrid rice.

The invention discloses a rice breeding method for polymerizing three disease resistance genes. The rice breeding method adopts combination of a molecular marker-assisted selection technology and an anther culture and conventional breeding technology to quickly polymerize a bacterial blight resistance gene Xa23, a rice blast resistance gene Pi-1 and a rice stripe virus resistance gene Stvb-i. The result shows that the disease resistance gene contained by an obtained anther culture material polymerizing the three disease resistance genes can carry out stable heredity and efficiently resist diseases. The rice breeding method creates a batch of new rice germplasm resources with stable resistance and provides a material basis for rice disease resistance and breeding.

The invention discloses a lucid ganoderma basidiospore germination culture medium, and a method using the lucid ganoderma basidiospore germination culture medium to germinate lucid ganoderma basidiospore. The lucid ganoderma basidiospore germination culture medium is prepared from a lucid ganoderma decocting liquid, a PDA (potato dextrose agar) powder and water. The lucid ganoderma basidiospore germination method has the advantages that the germination rate of lucid ganoderma basidiospore is 10% or above, and is even up to 20%, so as to obtain lucid ganoderma spore monokaryontic strains; the problem of lower germination rate of lucid ganoderma basidiospore under the laboratory condition is solved, so as to lay a foundation for the monospore crossbreeding of lucid ganoderma.

The invention relates to a method for performing cross breeding of cymbidium by using a molecular marker and belongs to the technical field of biological breeding. The method is characterized by comprising the following cross breeding steps of: extracting a deoxyribonucleic acid (DNA) genome from the cymbidium by a cetyltrimrthyl ammonium-bromide (CTAB) method; screening a primer; selecting a cross parent; breeding the selected parent for blossoming; sampling parent flower powder to perform artificial pollination; and breeding the pollinated parent until a bred fruit pod is ripe. The method has the advantages of creating a new path for the manually selected breeding of a cymbidium variety, making selection from wild plants unnecessary, greatly improving parent selection efficiency, accelerating breeding process and promoting the innovation of a cymbidium germplasm resource. An inter-simple sequence repeat (ISSR) marking technique of the invention has the characteristics of high polymorphism level, simple operation, high repeatability, high stability and the like, can detect rich genetic diversity and select the cross parent which has an appropriate genetic distance and an excellent phenotype in a relatively visualized way in a molecular level so as to increase the success rate of crossing.

The invention relates to a protein DNL1 for controlling rice leaf morphology and root hair development, and a coding gene and application thereof. The rice DNL1 is a protein as the following 1) or 2): 1) protein with the amino acid sequence disclosed as SEQ ID No.2; and 2) protein subjected to substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid residues sequence disclosed as SEQ ID No.2 in the sequence table with the same activity as SEQ ID No.2. The invention also discloses a coding gene of DNL1, and an expression box, a transgenic cell line, a recombinant bacterium and the like containing the gene. The gene has important theoretical values for leaf and root hair generation mechanisms, and can be used in rice breeding to enrich the genetic diversity of the existing rice varieties. The rice DNL1 protein can be used for cultivating the rice narrow leaf species and forming ideal rice plant types.

The present invention provides a rice genome-wide breeding chip and its application. The rice genome-wide breeding chip of the present invention is Rice60K, which is a SNP chip made based on Infinium chip manufacturing technology. Each chip can simultaneously detect 24 samples, including 58,290 SNPs These marker sites have the DNA sequence shown in SEQ ID NO.1-58290. The chip can perform molecular marker fingerprint analysis on rice variety resources, perform genotype identification on offspring of hybrid populations, and identify variety authenticity. The analysis and screening of the genetic background of breeding materials and the association analysis of agronomic traits have broad application prospects.

The invention belongs to the technical field of gene engineering, and particularly relates to a preparation method of a peanut mutant, a peanut mutant gene, protein encoded by the peanut mutant gene and an application. By designing SgRNA T1-T4, a CRISPR / Cas9 genome editing system is constructed, a Cas9 enzyme guided by sgRNA cuts a peanut desaturated fatty acid enzyme gene AhFAD2A at a gene target, and gene sequence insertion or base mutation is generated after DNA repair, so that targeted mutation of the peanut desaturated fatty acid enzyme gene AhFAD2A is completed, the gene cannot synthesize a functional desaturated fatty acid enzyme and cannot catalyze oleic acid dehydrogenation to generate linoleic acid, peanuts with high oleic acid content can be obtained, and the quality of peanut varieties can be improved.

The invention discloses a corn population artificial synthesis method for genetic breeding. The invention aims to breed a corn species having the advantages of high yield, wide application range, strong disease resistance, and quick dehydration, moreover, the corn should be simply or mechanically cultured and harvest in production in mountainous region of southwest China; and the group is established by selecting the advantages of germplasm materials and carrying out multi-stresses of high strength and multi-area complicated ecological environment stress. The tropic corn Suwan germplasm is themain material, the shortages of the tropic corn Suwan germplasm such as slow dehydration, low seeding rate, bad economical character, and the like are overcome; hybrid seeds suitable for future production are obtained; and the method has an important meaning for the corn breeding and genetic research in the mountainous region of southwest China.

The invention discloses an indirect ELISA (enzyme-linked immuno sorbent assay) kit for detecting a human toxoplasma gondii antibody and a preparation method of the indirect ELISA kit, and belongs to the field of biotechnology. The kit comprises a recombinant antigen GRA15 pre-coated plate, a sample diluent, a positive control, a negative control, an enzyme conjugate, a blocking solution, a washingsolution, a color developing solution and a terminating solution, wherein the amino acid sequence of a recombinant antigen GRA15 is shown in SEQ ID NO: 1. Compared with the existing detection technology, the indirect ELISA detection method has the advantages of being simple to operate, good in repeatability, economic and suitable for large-scale sample detection. Toxoplasma gondii Chineses1 strain GRA15 protein isolated in China is used as the coating antigen of the indirect ELISA method, and the kit has higher specificity for detection of toxoplasma gondii infection of Chinese people.

The invention relates to a prawn low dissolved oxygen resistance family selection method, and belongs to the technical field of prawn stress-resistance trait selection. The method comprises the steps of base population establishing; F1 generation family establishing; same pool culturing; low dissolved oxygen resistance testing; F2 generation family establishing; continuous generation selecting, wherein different sources of parent prawns are selected to establish a full / half-slib family through directional mating, common environmental breeding is performed, 94 h low dissolved oxygen stress is performed, parent selecting and remaining are performed according to the low dissolved oxygen resistance test result and the growth measure result, after four generations of continuous selection, the family of distant genetic relationship is selected from the families with excellent growth performance and strong low dissolved oxygen resistance performance, the male and female prawns which are big in individual, strong in vigor and normal in gonad development are selected to serve as parent to breed as good varieties. According to the method, the problem occurring in prawn high-density culturing that the prawns are dead caused by the fact that the prawn growth is influenced by low dissolved oxygen is solved, and the development of the prawn high-density culture industry is greatly promoted.

The invention discloses a treatment method of sweet potato germplasm resources collected in the field. The treatment method starts with the links of field collection of seed seedlings, seedling treatment and packaging, hydroponic preservation of seed seedlings, transportation of the seed seedlings, isolation transplanting, post-planting management and the like. With the method, the survival rate of the sweet potato germplasm resources collected in the field is greatly increased, the spread of sweet potato diseases and insect pests is effectively avoided, and field collection and treatment of the sweet potato germplasm resources are realized; a foundation is laid for enriching sweet potato germplasm resources and breeding of breakthrough varieties of sweet potatoes.

The invention discloses a breeding method for a golden yellow adductor muscle bay scallop new strain. New bay scallop products ''Zhong Ke Red'' and ''Zhong Ke NO.2'' serve as core collection groups, individuals with the adductor muscle being golden yellow are selected from the core collection groups as parent oysters so as to construct self-fertilization families, and the character of the golden yellow adductor muscle is subjected to rapid purification through family selection; the ''Zhong Ke Red'' self-fertilization family and ''Zhong Ke NO.2'' self-fertilization family are selected, wherein the ''Zhong Ke Red'' parent oysters and ''Zhong Ke NO.2'' parent oysters are the same in number, the hybridization group based on the two new products is constructed, the stability of the golden yellow character of the adductor muscle of the hybridization group is verified through breeding in different sea areas, and the golden yellow adductor muscle bay scallop new strain is obtained. The golden yellow character of the adductor muscle is rapidly purified through the family self-fertilization method, and the genetic diversity of the breeding strain is enriched by adopting a method of hybridization between varieties; and the nutritive value of the bay scallop is obviously increased, the product additional value and the breeding enterprise economic benefits are increased, and market prospects are wide.

The invention relates to a high-efficiency mutagenesis method of nicotiana tabacum L. pollen. The high-efficiency mutagenesis method comprises the following steps of putting nicotiana tabacum L. pollen into a liquid medium under an aseptic condition to carry out mutagenesis treatment by 0.1 to 0.2% of ethyl methanesulfonate (EMS) in a dark place at a temperature of 30 to 32 DEG C, carrying out chromosome doubling treatment by a NLN-16 liquid medium in a dark environment at a temperature of 30 to 32 DEG C, putting the nicotiana tabacum L. pollen obtained by the previous step into a NLN-13 liquid medium to carry out embryo induction culture in a dark environment at a temperature of 24 to 26 DEG C, carrying out shake culture by a shaking table in a dark environment at a temperature of 24 to 26 DEG C when an immature embryo appears, and putting the immature embryo growing a cotyledon into a solid medium to carry out subculture, wherein during a transplanting season, after seedling hardening, a seedling obtained by the high-efficiency mutagenesis method can be transplanted in a field for mutant character identification. The high-efficiency mutagenesis method overcomes dominant and recessive barriers of gene expression, is beneficial for selection, shortens greatly a mutant character stabilization period, saves breeding generation time, and has the characteristics of wide variation range, high mutagenesis frequency and the like.

The invention discloses an ELISA method and a kit for panoramic identification of intra-genus broad spectrum of a human norovirus antibody. The kit contains 28 Nov capsid proteins of human norovirus genotypes, wherein the Nov capsid proteins are respectively coated in an elisa plate. The intragenus broad-spectrum ELISA method for identifying the human norovirus antibody has the advantages of simplicity in operation, short period, high flux, good stability and the like, and can determine the broad-spectrum range of the norovirus antibody among different types in one step. The kit can be widely applied to scientific research, inspection and quarantine and other institutions and viral vaccine or antibody research and development companies.

The invention discloses a rice male sterile protein and a coding gene and application thereof. The protein is one of 1) a protein formed by amino acid residue sequences of the SEQ ID No.2 in a sequence table or 2) a protein which is formed after one or more amino acid residues in the amino acid residue sequences of the SEQ ID No.2 in the sequence table are substituted and / or deleted and / or added and has the same activities as the amino acid residue sequences of the SEQ ID No.2. The rice male sterile protein gene can be used for breeding male sterile rice, and makes contributions for removing male hybrid rice.

The invention discloses a Hibiscus cannabinus lead stress response gene EST-SSR primer group and a kit. The invention provides primer sequences of 22 Hibiscus cannabinus EST-SSR markers. The 22 EST-SSR markers have polymorphism in 138 Hibiscus cannabinus varieties / strains, Hibiscus cannabinus germplasms can be completely distinguished, and 138 materials are divided into three groups, which indicates that the Hibiscus cannabinus germplasms have obvious grouping ability to resist lead stress, so that a candidate marker is provided for screening Hibiscus cannabinus germplasm resources resistant to heavy metal lead stress and heavy metal lead resistant molecular marker-assisted breeding.

The invention relates to the technical field of crop breeding, and provides a breeding method for creating a new soybean germplasm. Natural open type natural hybridization is adopted, so that multi-gene recombination is achieved, genetic diversity is richer, complex procedures of artificial hybridization are overcome, and labor and time are saved. The method includes the steps that male parent soybeans and male genic male sterile female fertile female parent soybeans are planted, and harvesting is conducted to obtain basic soybeans and sterile plant grains; excellent soybeans are selected from the basic soybeans for planting, quality analysis is performed after harvesting, and breeding is completed; the plant height of the male parent soybeans and the plant height of the female parent soybeans are 80-90 cm independently; the grain protein is not less than 38% independently; and the grain fat content is independently greater than or equal to 18%. By hybridizing a soybean ms1 sterile plant and an excellent germplasm, the genetic basis of the soybean is separated and enriched, and the basic population is continuously optimized and improved. The provided breeding method for creating the new soybean germplasm is simple, rapid, high in selection efficiency, easy to master and convenient to operate.

The invention discloses a breeding method for a golden yellow adductor muscle bay scallop new strain. New bay scallop products ''Zhong Ke Red'' and ''Zhong Ke NO.2'' serve as core collection groups, individuals with the adductor muscle being golden yellow are selected from the core collection groups as parent oysters so as to construct self-fertilization families, and the character of the golden yellow adductor muscle is subjected to rapid purification through family selection; the ''Zhong Ke Red'' self-fertilization family and ''Zhong Ke NO.2'' self-fertilization family are selected, wherein the ''Zhong Ke Red'' parent oysters and ''Zhong Ke NO.2'' parent oysters are the same in number, the hybridization group based on the two new products is constructed, the stability of the golden yellow character of the adductor muscle of the hybridization group is verified through breeding in different sea areas, and the golden yellow adductor muscle bay scallop new strain is obtained. The golden yellow character of the adductor muscle is rapidly purified through the family self-fertilization method, and the genetic diversity of the breeding strain is enriched by adopting a method of hybridization between varieties; and the nutritive value of the bay scallop is obviously increased, the product additional value and the breeding enterprise economic benefits are increased, and market prospects are wide.

The invention discloses a method for selecting and breeding rice polymerized with three disease-resistant genes. The present invention combines molecular marker-assisted selection technology, anther culture and conventional breeding technology to quickly realize bacterial blight resistance gene Xa23 , rice blast resistance gene Pi‑1 stripe blight resistance gene Stvb‑i aggregation. The results showed that: the obtained disease-resistant genes contained in the flower culture materials that aggregated the three disease-resistant genes could be stably inherited and highly effective in disease resistance. The rice breeding method of the invention creates a batch of new rice germplasm resources with stable resistance, which provides a material basis for rice disease-resistant breeding.

The invention discloses a method for ecologically restoring heavy metal contaminated soil. According to the method, heavy metals are absorbed by a plant growth regulator, an artificial chelating agent and bamboo willows in a combining manner. The method disclosed by the invention can be used for absorbing heavy metal ions, namely Pb, Cu, Zn, Cd and Cr, by utilizing the high-quality bamboo willows which is fast in growth, high in resistance and good in material and accelerating the absorption of the bamboo willows on the heavy metal ions by adopting the plant growth regulator and the artificial chelating agent, so that the purpose of ecological restoration for 3-4 years can be achieved; after the heavy metal contaminated soil is ecologically restored through the bamboo willows, the concentration of the Pb, the Cu, the Zn, the Cd and the Cr which are contained in the soil can accords to the II class standard of the 'Environmental quality standard for soils (GB15618-1995)'.