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A method for breeding rice that aggregates genes for resistance to three diseases

A technology of disease-resistant genes and resistance genes, which is applied in the breeding method of rice containing three kinds of disease-resistant genes and its application field, and can solve problems such as being susceptible to environmental influences, long breeding cycles, and identification results errors

Inactive Publication Date: 2016-08-24
天津市农作物研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional breeding has a long cycle, low efficiency, and consumes a lot of manpower and material resources. Direct selection is often used for target traits. However, many agronomic traits are not easy to observe, are easily affected by the environment, and their performance is unstable. Direct selection is difficult.
Traditional rice disease resistance breeding mainly relies on the identification of resistance and the selection of plant phenotype, which requires rich experience and several years or even ten years, and is limited by the availability of suitable pathogen strains and pathogenic conditions. Identification results are likely to cause errors, and even cause the loss of resistance genes, so the selection efficiency is low and the cycle is long

Method used

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  • A method for breeding rice that aggregates genes for resistance to three diseases
  • A method for breeding rice that aggregates genes for resistance to three diseases
  • A method for breeding rice that aggregates genes for resistance to three diseases

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Embodiment 1

[0257] A method for breeding rice that aggregates genes for resistance to three diseases, comprising:

[0258] (1) Polymerization of genes resistant to two diseases: BG152 containing Xa23 gene for resistance to bacterial blight and R118 containing gene Pi-1 for resistance to blast Sexual hybridization was carried out to carry out double disease resistance gene aggregation, and obtained F containing Xa23 gene for resistance to bacterial blight and Stvb-i gene for resistance to stripe leaf blight respectively. 0 Seeds and F containing blast resistance Pi-1 gene and stripe leaf blight Stvb-i gene 0 seed;

[0259] (2) Aggregation of genes resistant to three diseases: using molecular markers to identify the F 1 Plants and F1 plants containing rice blast resistance Pi-1 gene and stripe leaf blight Stvb-i gene were used to identify true and false hybrids, and then the two true hybrids with double resistance and disease resistance genes were crossed with each other to obtain resista...

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Abstract

The invention discloses a method for selecting and breeding rice polymerized with three disease-resistant genes. The present invention combines molecular marker-assisted selection technology, anther culture and conventional breeding technology to quickly realize bacterial blight resistance gene Xa23 , rice blast resistance gene Pi‑1 stripe blight resistance gene Stvb‑i aggregation. The results showed that: the obtained disease-resistant genes contained in the flower culture materials that aggregated the three disease-resistant genes could be stably inherited and highly effective in disease resistance. The rice breeding method of the invention creates a batch of new rice germplasm resources with stable resistance, which provides a material basis for rice disease-resistant breeding.

Description

technical field [0001] The invention relates to a breeding method and application of rice strains. More specifically, the invention relates to a rice selection method and its application of polymerizing genes for resistance to three diseases. Background technique [0002] Rice is the largest grain crop in my country, and it is also the grain crop with the highest yield per unit area. The perennial planting area is less than 30% of the grain crops, but the output accounts for more than 40% of the total grain output; more than 60% of the population in my country takes rice as the staple food, especially The number of urban and rural residents whose staple food is japonica rice is increasing. Therefore, the development of rice production plays a pivotal role in solving my country's food security supply. [0003] In rice production, diseases are important limiting factors for high and stable rice yields, among which bacterial blight (a rice bacterial disease caused by the patho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 孙海波邹美智任洪岩王景余李艳萍冯瑞光
Owner 天津市农作物研究所
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