59results about How to "Avoid inbreeding" patented technology

The invention provides a method for cultivating earthworms. The method comprises the steps that first, a base box body is sequentially filled with river stones, gravel and silver sand, an earthworm cultivation box is placed on the base box body, organic waste mixtures are arranged in the earthworm cultivation box, then earthworm seedlings are put into the earthworm cultivation box, after the earthworms are cultivated for 30 days, organic waste mixtures are continuously added into the earthworm cultivation box until the earthworm cultivation box is filled with the organic waste mixtures; earthworm cultivation boxes are continuously placed on the earthworm cultivation box filled with the organic waste mixtures, after the earthworm cultivation box at the top layer is filled with the organic waste mixtures, the earthworm cultivation box at the bottom layer is drawn out, and the earthworm cultivation box is placed back to the top layer after earthworm cast in the earthworm cultivation box is cleaned away to achieve circulation cultivation. The method for cultivating the earthworms has the advantages that the facts that earthworms are bred from generation to generation, are inbred and are in variety degeneration are avoided, and breed conservation and reproduction of the earthworms can be better achieved. Adult earthworms are harvested conveniently, the living and incubation environment of the earthworms is not damaged, the incubation rate is guaranteed, and the method is suitable for earthworm industrial large-scale cultivation.

The invention discloses a high strength selecting and cultivating method of excellent stichopus japonicus seeds. The high strength selecting and cultivating method comprises the steps of carrying out cultivation, spawning or spermiation promotion, and hybridization on parent stichopus japonicuses from at least four different geographical groups, and carrying out selective breeding at different degrees in different periods according to a quantitative genetics method. By combining two traditional and effective methods of hybridization breeding and selective breeding, the invention preserves excellent properties of the stichopus japonicus and also avoids inbreeding; because the cultivated stichopus japonicuses have excellent properties of different groups, the cultivated stichopus japonicuses have the advantages of fast growth speed, strong stress resistance and the like; and the invention not only realizes seedling pre-culturing of the stichopus japonicus to meet market demand in advance, but also prolongs an effective growth period of the stichopus japonicus within a year, shortens cultivation time, and improves economic benefit; therefore, the inveniton is suitable for large-scaled factory production.

A breeding method for the new variety of the carp relates to a breeding method of a new variety of the fish, which resolves the problems of the prior group breeding method for the culture and the cultivation of the carp that is easy to have the inbreeding, which leads to the decrease of the economic character and the production performance; the intraspecific hybridization method has great randomness, the probability for breeding an excellent variety is low and is easy to have the inbreeding. The breeding procedures are: 1. performing the phenotypic character selection; 2. detecting the genetic information of the parent strain; 3. performing the reproduction grouping according to the genetic information; 4. cultivating the filial generation to be matured; 5. adopting the offspring carp as the new generation of the breeding parent strain and repeating the operation procedures from 1 to 4 until the carp meets the character design requirements of the new variety. The present invention always uses the molecular marker to detect the genetic composition of the parent strain and the filial generation in the selection process of the new carp variety, thereby avoiding the inbreeding; moreover the character of the new variety of the carp is developed in the designed direction, the emergence probability of the excellent new variety is high; therefore, the economic character and the production performance of the carps are obviously improved.

A method for building pedigree of marsupenaeus japonicus and breeding relates to the breeding of the penacus orientalis. The method comprises the following steps: building the base population; building the F1 generation pedigree; cultivating in a same pond; building the F2 generation close cousins pedigree; after growing to 3 cm, cultivating in the same pond, and selecting the breeding parents while reaching the specification of goods to generally build 8-16 close cousins pedigrees, wherein each close cousins pedigree retains 5-8 fullsibs family, the total number of the breeding pedigrees is 60-80, and the breeding parents in each pedigree are continuously bred to mature parents; and adopting a continuous multi-generation hybrid system to continuously build the close cousins pedigree of the next generation until to reach the breeding target and form the new fast growing variety. The invention is a method for building close cousins pedigree of marsupenaeus japonicus and breeding, especially can be applied to the inheritance breeding of the marsupenaeus japonicus, and has important significance for the breeding design of the marine animals which are not suitable for the fine breeding mode.

The invention relates to a method for breeding carp quickly and efficiently. The method is characterized by comprising the following culture steps of: establishing 80 first breeding families by taking carp and Yellow River carp as basic breeding colonies, hatching fish fry in net cages and performing early culture; injecting radio frequency marks into abdominal cavities of 50 fish fry selected from each family by using a syringe and individually marking; evaluating the breeding value of each fish fry by using ASReml software and according to a BLUP method; performing matched breeding on male fish with the evaluated breeding values in the first ninety places and female fish with the evaluated breeding values in the first ninety places, and establishing second breeding families; performing matched breeding on 20 male fish with the evaluated breeding values closest to 0 and 20 female fish with the evaluated breeding values closest to 0, and establishing second control families; and after the female fish are matched with the male fish, transferring fish nests with oosperms into hatching net cages to hatch to obtain the second generation of fish fry. By the method, the carp can be bred quickly and efficiently; inbreeding depression is avoided; and breeding can be performed for a long time.

The invention relates to a rapid and efficient method for breeding tilapias, which is characterized by comprising the following steps of: utilizing GIFT strain Nile tilapias of 60 families introduced from the Malaysia Worldfish Center as a basic breeding colony; separating tilapia fries of different families into different net cages for breeding; respectively selecting 30 fries from each family, and injecting a radio frequency identification to a fry abdominal cavity with an injector to identify individuals; breeding the fries before a propagation season of the next year, and measuring body weight, body length, body height and body thickness data of all identified tilapias; estimating a breeding value of each fringetail according to a BLUP (Best Linear Unbiased Prediction) method by utilizing ASREML software; selecting male tilapias with the estimated breeding values of the top 60 and female tilapias with the estimated breeding values of the top 120, and carrying out nest mating propagation in the ratio of 1:2 of the male tilapia to the female tilapia to establish a family of the second generation; taking out oosperms from the oral cavity of the female tilapia which spawns, and transferring the oosperms into a hatcher to carry out hatching of running water. The rapid and efficient method for breeding the tilapias can avoid the problem of inbreeding depression caused by inbreeding, thereby being capable of carrying out breeding for a long period of time.

The invention discloses a cement pit breeding method of langoustine, comprising the steps of seed selection, mating, hatching, juvenile langoustine breeding and langoustine seed catching. According to the invention, the seed selection standard of the parent langoustine is strict, and the old, weak, sick and disable parent langoustine are sifted out, thereby laying foundation for breeding health juvenile langoustine with high quality; since the parent langoustine used for breeding is different in resource, inbreeding is avoided, and the quality of the juvenile langoustine is ensured; oogenesis parent langoustine refers to oogenesis parent langoustine laying egg in the same period, the yield is high and the langoustine seeds are kept consistent in size; the disease resistance of the langoustine seed is strong, the loss of langoustine workers is reduced, and the cultivation benefit is increased; furthermore, according to the invention, parent langoustine transfer is simple and convenient, the langoustine seed catching is simple and feasible, and the labor intensity is greatly reduced.

The invention relates to the technical field of breeding of livestock and poultry, and especially relates to an efficient goat breeding method based on meat properties. The method comprises the following steps: determination on body weight, DNA sample acquisition, primer synthesis, template PCR amplification of a primer, genotype determination and genotyping, sequencing verification on individual PCR products with different genotypes, individual MBLUP heredity assessment, matching and establishing of a family, formation of a core colony through colony continuous-progeny selection, and manual dispersion of excellent genes. By determining body weight and detecting molecular marker genotype, MBLUP technology is employed for evaluating breeding value of breeding goats so as to perform selection, body-weight determination and genotype detection are performed on selected progeny according to a designed selection target, and the MBLUP method is used to perform colony continuous-progeny selection, so that finally a new variety of north Guizhou Ma goat for meat is formed. By taking selection of north Guizhou Ma goat as an example, the body weight of rams and ewes in a selected north Guizhou Ma goat core colony for meat is improved by 14.12 Kg and 9.61 Kg compared with a basic colony.

The invention discloses a method for keeping the genetic diversity of Jian carps. The method comprises the steps of individual tagging, template creating, genetic typing, family building, breeding file establishing and parent updating. Compared with the prior art, inbreeding of the Jian carps can be effectively avoided, the genetic diversity of Jian carp groups is kept and increased, and genetical characterization decline of the Jian carps is slowed down; in addition, breeding is assisted through molecular markers, a breeding file is established, genetic distances of the groups are analyzed by combining all parameters in the file, and therefore dominant groups are selectively reserved, and descendants excellent in genetic characterization are obtained through mating.

The invention relates to a method for breeding high-temperature-resistant Paralichthys olivaceus new varieties and belongs to the technical field of agricultural mariculture. In the method, Paralichthys olivaceus half sibs family and sibs family with a clear pedigree are established; multi-generation breeding is performed on the Paralichthys olivaceus family at half lethal temperature; inbreeding possibly occurring in the breeding process and tolerance of the Paralichthys olivaceus at different growth stages of juvenile fish and adult fish to the lethal temperature are taken into full consideration; and screening is repeated for two or more times, so that the high-temperature-resistant Paralichthys olivaceus new varieties can be bred effectively, and the problem that the Paralichthys olivaceus in some provinces in south China are difficult to aestivate.

The present invention relates to a method for the optimization of the fish variety, in particular to a method for the optimization of the carp variety, which resolves the problems that the inbreeding rate is high in the prior carp breeding cultivation and the intraspecific hybridization cultivation, thereby leading to the genetic degradation of the group and the decrease of the economic character and the production performance. The breeding procedures are: 1. performing the phenotypic character selection; 2. detecting the genetic information of the parent strain; 3. performing the reproduction grouping according to the genetic information; 4. the parent strains in each of the breeding group are free to mate with each other to get an optimized carp variety. The method of the present invention can avoid the inbreeding, so the variety can be optimized and the economic character and the production performance of the carps are obviously improved.

The invention discloses a rotational mating breeding method for pinctada fucata groups. The method comprises the following steps of: collecting at least three pinctada fucata groups from different sources, and constructing an F1 generation diallel crossing group; before a breeding season, randomly sampling over 1,000 individuals from every each group for performing character measurement, computing a breeding character average value or a multi-character comprehensive selection index, sequencing according to sizes, and at least selecting five groups serving as cone breeding groups by adopting a last-one-out system; selecting largest female and male individuals from every each selected group, mating male individuals of a No.1 group with female individuals of a No.2 group, mating male individuals of a No.2 group with female individuals of a No.3 group, and so on, and rotationally mating, wherein the ratio of female individuals to male individuals is 2:1, and at least four male individuals are selected; and breeding generation after generation to over five generations. Due to the adoption of the method, high selection strength is realized, genetic gain is increased, and the genetic improvement of every each generation of the bred pinctada fucata can be over 15 percent.

The present invention relates to a method for recovering an underwater ecosystem of a river. In particular, the method for recovering an underwater ecosystem of a river comprises a first step of constructing an upstream blocking wall at the same level as the bottom of a river such that the bottom of the river is positioned in the same line as upper surfaces of the upstream blocking wall; a second step of constructing a downstream blocking wall at the same level as the bottom of the river at a position spaced apart from the upstream blocking wall by a predetermined distance such that the bottom of the river is positioned in the same line as an upper surface of the downstream blocking wall; and a third step of dredging the bottom of the river in a predetermined depth according to a planned depth of water of the river and constructing a reservoir between the upstream and downstream blocking walls to release various kinds of underwater organisms suitable to regional characteristics, wherein the first to third steps are repeated to form a plurality of pond regions along the river. The method of the present invention further comprises a fourth step of constructing shallow regions for water purification between the plurality of pond regions. Therefore, there are advantages in that the underwater ecosystem of a river can be recovered and maintained healthily and the contamination of river can be prevented through the improvement of natural purification capability of the river, whereby a natural ecosystem including the ecosystem of an existing river can be kept more healthily and the river may be thus easily utilized as a water-friendly space.

The invention relates to an identity card coding method for global pets and other artificial fed animals. The identity card coding method comprises the following steps of: determining national codes according to birthplaces of pets or residence places of pet feeders; determining regional codes inside one state; determining a postal service delivery region code; determining birth date codes according to birth times of the pets; determining digital sequence codes of the pets; determining gender codes of the pets; determining category codes of the pets; determining species codes of the pets; and sequentially combining the codes to generate identity codes of the pets and other artificially fed animals. According to the coding scheme designed by the invention, nations, regions in the state, postal service delivery regions, birth dates, numerical codes, genders, category, species and other information can be easily distinguished from the codes. Identity codes of the pets in different states and different species are unified. According to the codes, outbreeding and inbreeding of similar pets can be effectively avoided.

The invention discloses a breeding method for maintaining the excellent traits of Procambarus clarkii. The method belongs to the field of animal molecular breeding, and specifically relates to a breeding method for maintaining the excellent traits of Procambarus clarkii. Through parental selection and cultivation, establishment of family, hatching and seedling, breeding of a family with excellenttraits, calculating the kinship between different individuals, re-selecting parents to cultivate, the excellent traits of stable Procambarus clarkii can be cultivated after multiple generations of breeding. The method lays a foundation for the construction and breeding of large-scale families, which is helpful for breeding new strains with excellent growth traits.

The invention relates to a poultry variety preservation method which is implemented by selectively breeding by turns among family generations. The method comprises the following steps: (1) original variety preservation populations are divided into a plurality of families with appropriate male / female matching ratio, and the families are numbered from No.1 to No.n; (2) during the next generation breeding process, a rooster in the No.1 family is mated with a hen in any one of the N0.2 to the No.n families, a hen in the No.1 family is mated with a rooster in any one of the N0.2 to the No.n families, the rooster and the hen of two different families are mated, and the genetic diversities of the rooster and the hen of the two mating families are different; (3) the previous step of next generation breeding is repeated, the two families for mating are different at each time of breeding, and the genetic diversities of the rooster and the hen of the two mating families are different; (4) the sub-breed of rooster and the sub-breed of hen are re-organized to form a new family according to the family tree; and (5) the step (1) to the step (4) are repeated. The invention can ensure that the biological characteristics of the variety preservation population remain relatively stable.

The invention discloses a breeding method of black goats. The breeding method comprises the following steps: 1) performing grazing; 2) performing dry lot feeding; 3) preparing feeds which are required to be high in nutritive value, have sufficient protein, vitamins and inorganic salts, and be easy to digest and good in palatability; and 4) performing mating with the following requirements: rams need to keep better than average body conditions, are sexually active, are fed in a separate sheep pen, are grouped separately for grazing, activity and supplementary feeding, and must not be mixed with ewes, the ewes need to be fattened for rejuvenation before mating, thin and weak ewes need proper increase of nutrition, and the ewes need self-help feeding with green and coarse feeds, only need about 0.2kg of concentrated feeds as supplementary feeding every day to prevent abortion, should be fed with more concentrated feeds at late stages of pregnancy due to quick development of fetuses, should be fed with certain succulent feeds about 10 days before delivery, and need nutrition strengthening in the lactation period to guarantee sufficient milk. The breeding method of the black goats, disclosed by the invention, by virtue of grazing, dry lot feeding, feed preparation, and mating, solves the problems of long growth cycle, poor mutton quality, high feed cost and the like in the prior art.

A river ecosystem restoration method, including the following stages: the first stage of maintaining the same height between the upstream partition wall and the river bottom in order to make the end face of the river bottom and the upstream partition wall on the same line; The second stage of maintaining the same height between the downstream partition wall and the river bottom in order to make the end face of the river bottom and the downstream partition wall on the same line; between the aforementioned upstream partition wall and the downstream partition wall according to the planned water depth of the river The third stage of the water storage area is to build a certain depth, and then form a water storage area that can release various aquatic organisms that meet the local characteristics. The first to third stages have formed multiple swamp areas; The fourth stage of the rapids zone of action. The present invention can not only restore and maintain the ecological system in the river water, but also prevent river pollution by improving the natural purification ability of the river, thereby making the related natural ecological system of the river ecological system healthier and making it easier to use the hydrophilic space environment.

The invention relates to a method for matching and breeding jian carps. The method comprises the following steps of: sampling blood or fin rays of a jian carp breeding population to extract genome DNA; detecting the genotype of SNPs (Single Nucleotide Polymorphisms) by a PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method constructed by taking the extracted genomeDNA as a template according six SNP sites related to jian carp weight increment; carrying out genotype determination by using a sepharose gel electrophoresis enzyme slitting strip; selecting individuals containing more than four genotypes with rapid weight increment as candidate parents; carrying out digital treatment on the candidate parents by using the PCR-RFLP sepharose gel electrophoresis enzyme slitting strip; analyzing by using genetic analysis software to obtain genetic distances between every two individuals; constructing an evolutionary tree; and selecting an individual which has a larger genetic distance and better gonad development and can enrich more than four genotypes with rapid weight increment. The method is helpful to improving the production performance of jian carps, avoiding inbreeding and accelerating the breeding process of jian carps.

The invention relates to a staged population selective breeding method of a Takifugu obscurus non-toxic pedigree, which is used for selecting unrelated artificial freshwater aquaculture parent fishes according to the population selecting standard and the individual selecting standard. The selective breading stage comprises a preoviposition period, an artificial reproduction period, a larva period, a postlarva period, a fishseed period, a commercial fish period and a prematuration period. The method comprises the following steps of: selecting in the continuous culturing period of three to four years according to the selecting standard of each stage to selectively breed individuals not containing tetrodotoxin; stabilizing the non-toxicity character generation by generation through at least two continuous selective breeding periods to eliminate the possibility of hereditarily carrying the tetrodotoxin, and then selectively breeding non-toxic populations with rapid growth and strong stress resistance; and judging whether the whole fishes and highly toxic organs comprising livers and ovaries of commercial fishes in various specifications are toxic or not, and selecting and preserving the non-toxic populations. The method has the advantages of simple and easy execution and rapid effect taking and is suitable for selectively breeding the populations in any scales of fish fry fields.

The invention discloses a technology for increasing the breeding survival rate of black goats. The technology includes the steps that 1, old and weak ewes are weeded out; 2, inbreeding is strictly prohibited, and rams are replaced once every year; 3, appropriate hybridization time is selected, wherein hybridization is performed in November every year; 4, feeding management is performed on pregnant ewes; 5, newly-born lambs are nursed; 6, lamb dysentery is prevented and treated; 7, feed supplementation and ablactation are performed in due time. By weeding out the old and weak ewes, replacing the rams, prohibiting inbreeding and controlling the hybridization time, it is guaranteed that plenty of forage is available for the lambs; by managing fodder of the pregnant ewes, it is guaranteed that the lambs have a good physique and the ewes have sufficient milk; by managing the lambs, balanced growth and development and good disease resistance of the lambs are guaranteed, and therefore the breeding survival rate of the lambs can be greatly increased.

The invention provides a duplex PCR method for identifying the genetic relationship of black carp. The method comprises the steps as follows: (1) extracting genome DNA of a black carp sample; (2) performing duplex PCR by 5 groups of microsatellite primers; (3) performing electrophoretic separation on a PCR amplified product on polyacrylamide gel; (4) counting genotype of the PCR amplified productand analyzing the genetic relationship between individuals. The 5 groups of microsatellite primers are sites with high polymorphism and clear strips and can realize genetic diversity detection and individual recognition of the black carp and identify the genetic relationship between individuals, inbreeding among black carp individuals can be avoided, and a the used time and cost are a half of those in traditional PCRs. Tests prove that the method can identify the genetic relationship of 30 black carps and accurately identify the genetic relationship between samples, and the genetic distances between the samples can be calculated according to identification results.

The invention relates to a breeding rabbit selection method capable of improving the breeding effect. A comprehensive selection index method is applied to achieve breeding rabbit selection, a weighingvalue is regulated according to the main character, breeding rabbits conforming to the breeding direction are selected to complete rabbit breeding, the rabbit breeding is optimized, and directional breeding is achieved. According to the rabbit breeding management system, the breeding rabbit stage growth information data can be completely recorded, same-generation and all-generation breeding rabbit designated index data transverse and longitudinal comparison is achieved, breeding rabbit selection is facilitated, the family tree relation among the zero, first, second, third and fourth generations can be visually queried, and inbreeding is avoided. The breeding rabbit breeding management system further comprises a breeding work daily management module for achieving the system management breeding progress, the schedule reminding function is achieved, omission or expire management items deserved to be processed in the breeding management process can be effectively avoided, and breeding management is performed in time and in order.

The invention discloses a three-dimensional water culture system. The three-dimensional water culture system comprises one or more multi-layer isolation mesh boxes and a water flow feeding system; thewater flow feeding system is used for delivering baits into the multi-layer isolation mesh boxes; the multi-layer isolation mesh box has multiple layers; each layer comprises multiple isolation spaces; the isolation spaces are used for culturing aquatic animals; the water flow feeding system comprises delivery pipes; each multi-layer isolation mesh box is vertically penetrated by one or more delivery pipes; each isolation space of each multi-layer isolation mesh box is communicated with a delivery pipe; and, when a pressurized water flow containing baits is input into the delivery pipe, the baits in the pressurized water flow enter the communicated isolation space through a ventilation hole formed in the delivery pipe. According to the three-dimensional water culture system in the invention, the multi-layer isolation mesh boxes are adopted; furthermore, the aquatic animals are isolated by utilization of the isolation spaces; therefore, the utilization rate of water spaces is increased; the culture density and the survival rate of the aquatic animals can be greatly increased; furthermore, the cost is relatively low; and thus, the three-dimensional water culture system in the invention has relatively high economic benefit.

The invention discloses a molecular marker-assisted breeding method of a new variety for rapid growth of trachinotus ovatus. According to the method, by efficiently integrating technologies such as individual phenotype value selection, microsatellite molecular marker, additive genetic correlation matrix, linear hybrid model, quantity genetics, individual breeding value selection, gender specific molecular marker, inter-individual genetic distance analysis, group mating scheme and the like, the problems of slow fry growth, low survival rate, poor stress resistance and disease resistance, germplasm degeneration and the like caused by difficulty in candidate parent genetic evaluation and optimal selection and blind inbreeding due to unclear genetic relationship and sex in existing trachinotus ovatus genetic breeding work can be effectively solved.

The invention belongs to the breeding technical field, and specifically discloses a guinea pig breeding method comprising the following steps: 1, species selection: selecting excellent individuals with strong arms and legs, flat back and wide ass and well grown genitals from a batch of guinea pigs of 3-month-old, and using same to serve as male and female guinea pig species; 2, breeding: using culture forage to feed the guinea pigs at the following quantity and frequency: 140-180g / time / pig, and 2times / day; 3, hybridization: allowing pubescent male and female guinea pigs to hybridize, breeding newly produced guinea pig babies to 4-month-old, and determining species. The guinea pig breeding method strictly selects species, uses special formula culture forage to feed the guinea pigs, thus improving guinea pig species breeding capability, guinea pig baby survival rate and growth speed.

The invention provides a method for cultivating earthworms. The method comprises the steps that first, a base box body is sequentially filled with river stones, gravel and silver sand, an earthworm cultivation box is placed on the base box body, organic waste mixtures are arranged in the earthworm cultivation box, then earthworm seedlings are put into the earthworm cultivation box, after the earthworms are cultivated for 30 days, organic waste mixtures are continuously added into the earthworm cultivation box until the earthworm cultivation box is filled with the organic waste mixtures; earthworm cultivation boxes are continuously placed on the earthworm cultivation box filled with the organic waste mixtures, after the earthworm cultivation box at the top layer is filled with the organic waste mixtures, the earthworm cultivation box at the bottom layer is drawn out, and the earthworm cultivation box is placed back to the top layer after earthworm cast in the earthworm cultivation box is cleaned away to achieve circulation cultivation. The method for cultivating the earthworms has the advantages that the facts that earthworms are bred from generation to generation, are inbred and are in variety degeneration are avoided, and breed conservation and reproduction of the earthworms can be better achieved. Adult earthworms are harvested conveniently, the living and incubation environment of the earthworms is not damaged, the incubation rate is guaranteed, and the method is suitable for earthworm industrial large-scale cultivation.

The invention relates to an earthworm culture facility and a method for culturing earthworm and treating organic waste. The earthworm culture facility comprises one shaft body or combination of a plurality of shaft bodies for culturing earthworm, wherein a hollow shaft is arranged on the shaft body. The method comprises the following steps: (1) putting earthworm feed and earthworm in the shaft of the shaft body; (2) feeding the earthworm in the shaft for 30-40 days, controlling the temperature in the shaft to be 18-25 DEG C, and keeping ventilation; and (3) taking the earthworm and earthworm manure out of the shaft body, and separating to obtain the earthworm and earthworm eggs. By adopting the facility, earthworm culture can be industrialized and mechanized, and season influence, especially winter influence, can be eliminated. The facility has the characteristics of harmlessness, energy conservation, low cost and the like.

The invention provides a leech culture method comprising the steps of placing hatched leech younglings into a rest culture tank; when the leech younglings are just hatched, putting starter feed into the rest culture tank; performing graded raising once on the leech younglings every time the leech younglings grow for one instar; increasing the water level in the rest culture tank with the growth of the leech younglings; increasing the quantity of feed delivered into the rest culture tank with the growth of the leech younglings; purifying external water by using a water preparing pool and feeding the water into the rest culture tank. The leech culture method can prevent inbreeding and guarantee first feed taking of leech younglings; the method enables leeches to live in an environment suitable for growth consistently, thereby increasing the surviving rate of leeches and increasing the economic benefit.