297 results about "Guinea pig" patented technology

Existing epiretinal implants for the blind are designed to electrically stimulate large groups of surviving retinal neurons using a small number of electrodes with diameters of several hundred μm. To increase the spatial resolution of artificial sight, electrodes much smaller than those currently in use are desirable. In this study we stimulated and recorded ganglion cells in isolated pieces of rat, guinea pig, and monkey retina. We utilized micro-fabricated hexagonal arrays of 61 platinum disk electrodes with diameters between 6 and 25 μm, spaced 60 μm apart. Charge-balanced current pulses evoked one or two spikes at latencies as short as 0.2 ms, and typically only one or a few recorded ganglion cells were stimulated. Application of several synaptic blockers did not abolish the evoked responses, implying direct activation of ganglion cells. Threshold charge densities were typically below 0.1 mC / cm2 for a pulse duration of 100 μs, corresponding to charge thresholds of less than 100 pC. Stimulation remained effective after several hours and at high frequencies. To demonstrate that closely spaced electrodes can elicit independent ganglion cell responses, we utilized the multi-electrode array to stimulate several nearby ganglion cells simultaneously. From these data we conclude that electrical stimulation of mammalian retina with small-diameter electrode arrays is achievable and can provide high temporal and spatial precision at low charge densities. We review previous epiretinal stimulation studies and discuss our results in the context of 32 other publications, comparing threshold parameters and safety limits.

The invention discloses an O type foot-and-mouth disease 146S antigen quantitative ELISA (enzyme-linked immuno sorbent assay) detection kit and a method for using the same. The kit comprises an ELISA plate, an O type foot-and-mouth disease standard reference antigen, a demulsifier, an O type foot-and-mouth disease rabbit antiserum, an O type foot-and-mouth disease guinea pig antiserum, a rabbit anti-guinea pig-horse radish peroxidase conjugate, a guinea pig antiserum dilute solution, a 25-fold PBST (phosphate buffer solution tween) concentrated solution, a carbonate buffer solution capsule, a citric acid-phosphate buffer solution tablet, an OPD (o-phenylenediamine) tablet, a stop solution, a plate sealing membrane, a moving liquid tank and a 96-mesh U-shaped dilution plate. The kit is an organic combination of a sucrose density gradient centrifugation method and an indirect sandwich ELISA method, integrates the advantages of the sucrose density gradient centrifugation method and the indirect sandwich ELISA method, is simple to operate and good in stability, is suitable for batch detection, can be used for distinguishing serum types, and is an ideal substitution method for antigen quantitative and vaccine efficacy detection.

Existing epiretinal implants for the blind are designed to electrically stimulate large groups of surviving retinal neurons using a small number of electrodes with diameters of several hundred μm. To increase the spatial resolution of artificial sight, electrodes much smaller than those currently in use are desirable. In this study we stimulated and recorded ganglion cells in isolated pieces of rat, guinea pig, and monkey retina. We utilized micro-fabricated hexagonal arrays of 61 platinum disk electrodes with diameters between 6 and 25 μm, spaced 60 μm apart. Charge-balanced current pulses evoked one or two spikes at latencies as short as 0.2 ms, and typically only one or a few recorded ganglion cells were stimulated. Application of several synaptic blockers did not abolish the evoked responses, implying direct activation of ganglion cells. Threshold charge densities were typically below 0.1 mC / cm2 for a pulse duration of 100 μs, corresponding to charge thresholds of less than 100 pC. Stimulation remained effective after several hours and at high frequencies. To demonstrate that closely spaced electrodes can elicit independent ganglion cell responses, we utilized the multi-electrode array to stimulate several nearby ganglion cells simultaneously. From these data we conclude that electrical stimulation of mammalian retina with small-diameter electrode arrays is achievable and can provide high temporal and spatial precision at low charge densities. We review previous epiretinal stimulation studies and discuss our results in the context of 32 other publications, comparing threshold parameters and safety limits.

The invention is a kind of polypeptide vaccine and the manufacturing method which can resist Asia one foot and mouth disease virus. The vaccine is prepared by that DNA array of B , T cell form location amino acid on VP1 and VP4 contact with coding form location albumen alone, or link with macromolecule of the carrier coding schedule location merged protein. The invention uses chemical method to synthesize the peptide gene of B cell and T cell form location on coding Asia1 type VP1 and VP4, and they are contacted, the gene is inserted with particle carrier alone or connecting with the carrier molecular, and transferred into bacteria express, and yeasted and gets the product.

The invention discloses a double antibody sandwich ELISA kit used for Seneca viral antigen detection and application thereof. The kit comprises a Seneca viral guinea pig anti-IgG enveloped elisa plateand an HRP marked Seneca viral rabbit anti-IgG. The HRP marked rabbit anti-IgG is used for replacing primary antibodies and secondary antibodies in traditional ELISA, so that the operating steps aresimplified. Simultaneously, the SVV guinea pig anti-IgG is enveloped to the surface of a solid-phase carrier by changing an enveloping stabilization technology, the preparation technology of an enzyme-labeled antibody diluent is changed, an enzyme-labeled antibody working solution can be stored stably without changing the activity and valence, and a double antibody sandwich ELISA kit for SVV antigen specific detection and a detection method thereof are established. The vacancy of making up SVV ELISA antigen detection is proposed, the problem that existing virus separation and detection for SVVantigens are low in repeatability and sensitivity and complex in operational program is overcome, and effective technical means is provided for SVV antigen detection.

The invention relates to application of maidenhair volatile oil in a medicine for treating rhinitis. A method comprises the step of grinding a whole herb of maidenhair and then extracting the maidenhair by a steam distillation method or a carbon dioxide supercritical fluid extraction method to form the volatile oil. No toxic reagent is used by the two extraction methods, and the extracted volatile oil has the characteristics of no toxicity, no residue, simple extraction technology and high extraction efficiency. A medicinal auxiliary material can be added into the volatile oil to prepare a nasal drop or a spray as the medicine for treating the rhinitis. Pharmacological experiments show that the volatile oil has a better pharmacological action on an allergic rhinitis model of a guinea pig, can obviously alleviate symptoms of a nose of the allergic rhinitis model of the guinea pig, reduces an ethological accumulative integral, and significantly relieves edema of a nasal mucosa of the allergic rhinitis model of the guinea pig.

The present invention discloses the cultivating technology of aseptic cavy and the feed formula. The cultivating technology includes abdominal incision to take out cavy embryo, raising and propagating in aseptic isolating apparatus, and providing delicious feed comprising protein, carbohydrate, fat, vitamins and minerals and Co-60 ray irradiated. The cavy thus raised has high survival rate and no microbe and parasite infection, and makes it possible to obtain correct and reliable experiment result. The present invention may be used widely in various research and production fields.

The invention discloses an encoding gene of porcine parvovirus VP2 protein, a method of prokaryotically expressing VP2 protein virus-like particles, and application of the method in vaccine preparation. Sequences are optimized, VP2 gene is artificially synthesized, the synthesized gene is inserted into pET28a vector, the gene and chaperone protein plasmids are co-transferred to BL21(DE3) host bacteria, the VP2 protein and chaperone protein are co-expressed to promote correct folding of the VP2 protein. Experiments prove that recombinant bacteria expressed VP2 protein can be self-assembled in vitro and has good immunogenicity; by immunizing mice and guinea pigs with the virus-like particle subunit vaccine prepared with the VP2 protein expressed herein, it is possible to induce the production of a high level of hemagglutination inhibition antibodies and neutralizing antibodies, and the vaccine can prevent guinea pigs from being affected by strong porcine parvovirus. The recombinant bacteria according to the invention can be utilized to efficiently prepare porcine parvovirus virus-like particles, the production cost is low, operation is simple, and biosafety is better.

The invention discloses a bovini Asia 1 / O type foot-and-mouth disease bivalent multi-epitope vaccine and a preparation method and an application thereof and belongs to the field of veterinary biological vaccines. The bovini Asia 1 / O type foot and mouth disease virus compound multi-epitope recombinant antigen is obtained by coupling dominant epitope of epidemic bovini O and Asia 1 type foot-and-mouth disease virus representative strains connected in series in China with a bovini IgG heavy chain constant region. Immune efficacy experiments verify that the bovini Asia 1 / O type foot-and-mouth disease bivalent multi-epitope vaccine prepared by the invention has good immunogenicity, and high level protective antibodies can be generated by the body induced by immune guinea pig or immune bovine. The inoculated vaccine is safe and harmless to immune animals. The vaccine is a novel vaccine which is abroad in prospect, supports matter storage and technical support to prevention and control of bovini Asia 1 / O type foot-and-mouth disease in China, and is of great importance.

The invention comprises novel polynucleotides, and related vectors, host cells, methods, and compositions, containing transcriptional enhancers providing very high levels of expression of operably-linked expressible nucleic acid sequences in eukaryotic cells. Advantageously the enhancers may be used in combination with their naturally-associated promoters and / or other genetic elements that increase transcription. The invention comprises eukaryotic expression vectors that are capable of providing increased levels of expression in many cell types over that obtainable from human or murine CMV IE enhancer / promoter elements.