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Metabonomics analysis method base on acute anaphylactic reaction

A technology of allergic reaction and metabolomics, applied in the field of pattern recognition analysis and evaluation of acute allergic reaction of animals, can solve the problem of low sensitivity and achieve the effect of sensitive results and easy operation

Active Publication Date: 2013-08-07
HUNAN INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of the traditional acute allergic reaction detection method, such as strong subjectivity and low sensitivity, and propose an acute allergic reaction guinea pig serum metabolome analysis based on ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry The method has the characteristics of being objective, sensitive and reliable.

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  • Metabonomics analysis method base on acute anaphylactic reaction
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  • Metabonomics analysis method base on acute anaphylactic reaction

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Embodiment 1

[0031] The metabolomics analysis method based on acute allergic reaction comprises the following steps:

[0032] (1) Divide clean-grade male guinea pigs (250-350g) into blank control group and acute allergic reaction group randomly. The acute allergic reaction group used bovine serum albumin (BSA) as a positive sensitizer, and gave animals 10mg / mL bovine serum albumin, 1.0mL / animal, intraperitoneal injection, once every other day, for 3 consecutive times; on the 14th day after the first sensitization On the first day (the first challenge) and on the 21st day (the second challenge), the toe vein injection (2.0ml / cause) was challenged. The blank group was given the same volume of normal saline;

[0033] (2) Before sensitization, after the third sensitization, and 30 minutes before and after the first and second challenges, the guinea pigs were subjected to orbital blood collection, left at room temperature for 10 minutes, centrifuged at 4°C and 3000rpm for 10 minutes, and the u...

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Abstract

The invention discloses a metabonomics analysis method base on acute anaphylactic reaction. The invention mainly starts from the systems biology, and applies a UPLC-QTof / MS technology platform for UPLC and SPE-RRLC-Q-TOF combination, and establishes an acute anaphylactic reaction guinea pig serum metabonomics evaluation model, and provides a basis for further research of medicament acute anaphylactic reaction. During analysis, the UPLC and SPE-RRLC-Q-TOF combination technology is used for detecting serum samples of normal guinea pig and experiment guinea pig with acute anaphylactic reaction generated by induction, and a metabolome spectrogram is obtained by an acquisition method after optimization, and a mode identification method suitable for evaluating animal acute anaphylactic reaction is established for analyzing the metabolome data, which establishes a base for subsequently and furtherly screening biological tag with characteristics and developing a new animal acute anaphylactic reaction detection method.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and biochemistry, and specifically relates to the optimization of a guinea pig serum metabolomics data collection method based on ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry and the collected metabolomics Data were subjected to a pattern recognition analysis method suitable for evaluating acute allergic reactions in animals. Background technique [0002] Anaphylaxis is a common adverse reaction. As early as 1902, drug anaphylaxis attracted the attention of experts, especially the acute allergic reaction caused by drugs seriously threatens the safety of human life. A British study showed that 50% of patients who died of anaphylactic shock were induced by drugs, and the average death time was 5 minutes after drug administration. There are 550,000 severe drug allergic reactions in U.S. hospitals each year. my country's ADR monitoring ...

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Application Information

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IPC IPC(8): G01N30/86
Inventor 蒋秋桃李文莉林海王蓉蓉丁野潘震宇李子珊刘晓娟
Owner HUNAN INST FOR FOOD & DRUG CONTROL
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