32results about How to "Sensitive result" patented technology

The invention discloses a PCR detection method for identifying germplasms of four populations of Pelodiscus sinensis, a primer group and a kit. The primer group comprises primers of SEQ No.1 / SEQ No.4, SEQ No.2 / SEQ No.5 and SEQ No.3 / SEQ No.6. The kit comprises a 2*TaqPCR premixed liquid, a 5U / mul enzyme BglI and 10*digestion buffer liquid A, a 5U / mul HpaII and 10*digestion buffer liquid B, a 5U / mul ClaI and 10*digestion buffer liquid C, 10mg / ml BSA, a contrast DNA template and a 10pM amplimer. The method comprises the following steps: carrying out PCR amplification of extracted DNA through using the primer group, digesting to obtain a digestion product, carrying out agarose gel electrophoresis, and comparing the obtained electrophoresis result with a standard electrophoretogram to obtain a result. The detection method has the advantages of simplicity, good reaction stability and repeatability, and cost saving.

The invention belongs to the technical field of biotechnologies and discloses a method for detecting MMP-9 by a fluorescence zymography imprinting technology. The method includes: 1) subjecting a to-be-detected serum sample to incubation centrifuge according to a gel protein concentration method to increase enzyme concentration of the serum sample by five times; 2) adopting a fluorescence zymography imprinting method for distinguishing various enzymes and proteins in the concentrated to-be-detected serum sample by electrophoresis according to molecular weights; 3) adopting a capillary imprinting method for transferring the proteins distinguished at the step 2) to the surface of a nitrocellulose membrane, and degrading a substrate pre-cured on the surface of the nitrocellulose membrane; 4) after degradation of the substrate, separating a fluorescence group from a quenching group to emit fluorescence under a specific excitation wavelength, and detecting fluorescence intensity to judge enzyme activity of MMP9 in the to-be-detected serum sample. On the premise that advantages of gelatin zymography are kept, serum is concentrated, and detection sensitivity is improved; by adoption of the fluorescence zymography imprinting method, experimental results are low in background, and high sensitivity and quantifiability of the results are realized.

The invention relates to the technical field of organic small molecule isotope labeling, in particular to a stable isotope mercapto compound labeling reagent, a synthesis method and application thereof. The isotope labeling reagent is [d0] / [D4]-acridone-10-ethyl-N-maleimide. The reagent adopts acridone as the isotope group and takes maleimide as the reaction group. The synthesis method includes: taking [d0] / [D5]-bromobenzene as the raw material, and carrying out two-step condensation reaction, hydroxylation reaction, helogenation reaction, Gabriel reaction and acylation reaction, thus obtaining the reagent. The stable isotope deuterium label obtained by the invention is subjected to separation and purification, then the isotope labeling position is table, the chemical purity is up to 99.0% or more, and the isotope abundance is up to 99.0% or more. The reagent can selectively label mercapto compounds, and has the advantages of fast labeling reaction rate, high labeling yield and high mercapto selectivity, etc.

The invention relates to a method for measuring residual quantity of thidiazuron and diuron as well as metabolites thereof in cotton, which comprises the following concrete steps: weighing 20.0g of sample; putting the sample into a conical flask with a plug; adding 50mL of acetonitrile and 10mL of distilled water; oscillating and extracting for 60min on an oscillator; adding 6.0-10g of sodium chloride into the filtered solution; oscillating for 2-5min; standing for layering; collecting the organic phase at the upper layer; dehydrating by anhydrous sodium sulfate; carrying out decompression concentration in a water bath at 40 DEG C for nearly drying; blow-drying by nitrogen gas; adding 10-20mL of ethyl acetate; then pre-leaching by adopting a C18 column and 5mL of ethyl acetate; sampling; leaching by 10mL of ethyl acetate; collecting the leacheate; carrying out decompression concentration in the water bath at 40 DEG C for nearly drying; blow-drying by nitrogen gas; metering the volume by 2mL of methanol; filtering by a filter membrane; and measuring by adopting a DAD detector. The measuring method of the invention simplifies the extracting steps, reduces the consumption of solvents such as toluene dichloride and the like, and reduces the interference of impurities in the sample by using solid phase extraction and purification so that the result is more accurate, sensitive and reliable.