39 results about "Saccharum arundinaceum" patented technology

The invention belongs to the field of genetic breeding. The invention particularly relates to an SSR molecular marker and method for identifying authenticity of filial generations of sugarcane and saccharum arundinaceum. The SaSSR molecular marker is amplified by adopting 10 primer pairs with nucleotide sequences as shown in SEQ ID NO: 1-20, an amplification product is detected by agarose gel electrophoresis, and the authenticity of the filial generation of sugarcane and saccharum arundinaceum is identified by analyzing strips. The SaSSR molecular marker disclosed by the invention is a group of specific SSR markers with different amplification bands between sugarcane and saccharum arundinaceum, uniform distribution of the markers in different chromosomes of saccharum arundinaceum is ensured, and the SSR molecular marker and method are particularly suitable for identification of sugarcane and saccharum arundinaceum high-generation materials.

The invention relates to a method for performing genome in-situ hybridization in a chromosome suspension (Genome in situ hybridization in suspension, GISHIS). The method comprises the steps of cell cycle synchronization, preparation of the chromosome suspension, preparation of a genomic probe, denaturation of samples and genomic probe and genome in-situ hybridization in the suspension. Dripping piece microscopic examination is carried out, if a green fluorescence signal is generated in the suspension, a saccharum arundinaceum chromosome is determined, so that it is indicated that green fluorescence is successfully marked onto the saccharum arundinaceum chromosome. A flow cytometry is utilized to achieve the separation of the saccharum arundinaceum chromosome specially marked with the greenfluorescence signal in the chromosome suspension and perform the related sequencing study, thereby providing a new, effective and feasible method for studying a complex polyploid saccharum arundinaceum genome. The method is applied to the genome in-situ hybridization in the chromosome suspension of intergeneric hybridization offspring in various crops.

The invention discloses application of a Chinese herbal preparation to treatment of chronic renal failure. The Chinese herbal preparation comprises the following raw materials containing effective constituents by weight: 60 to 90 parts of herba elsholtziae ciliatae, 57 to 87 parts of radix paeoniae alba, 55 to 85 parts of radix morindae officinalis, 53 to 83 parts of saccharum arundinaceum, 50 to 80 parts of limonium sinense, 45 to 75 parts of salvia miltiorrhiza, 42 to 72 parts of herb of thymeleaf speed welt, 40 to 70 parts of rosa multiflora Thunb., 38 to 68 parts of lysimachia paridiformais Franch., 35 to 65 parts of herb of Japanese conehead, 33 to 63 parts of carthamus tinctorius, 31 to 61 parts of root and rhizome of bloodred iris, 30 to 60 parts of polygonum runcinatum, 26 to 56 parts of fiveleaf akebia fruit, 25 to 55 parts of rhizome of elderleaf rodgersflower, and 20 to 50 parts of aleuritopteris argentea. The Chinese herbal preparation has no toxic or side effect, is obvious in renal failure treating effect, low in cost, fast in effect taking and lasting in curative effect, and has no adverse reaction.

The invention relates to a method for screening drought-tolerant mutant sugarcane strains, which belongs to the technical field of sugarcane molecular breeding, and specifically relates to a method for selecting drought-tolerant sugarcane plants. A method for breeding drought-tolerant sugarcane plants, co-cultivating sugarcane embryogenic suspension small cells with a suitable concentration of the total DNA solution of Banmaea, culturing the cultures on MS medium with different concentrations of PEG, and improving the proliferation of callus, Repeated drought-resistant screening for differentiation, and SRAP molecular detection of mutant strains, and finally selection for drought-resistant stress in greenhouses, and finally drought-resistant sugarcane plants were selected. The method of the invention shortens the time for sugarcane breeding, has simple operation and low price, and simultaneously provides a new way to break through close hybridization of Saccharum genus and species, and the obtained sugarcane plants have better drought-tolerant characteristics than the control.

The invention provides a screening method of a saccharum arundinaceum breeding material. The method comprises the following steps: collecting saccharum arundinaceum resources, and performing variation analysis of phenotypic character and correlation analysis among characters on the resources; extracting the genome DNA of saccharum arundinaceum as a template, adopting SCoT labeled primers to perform amplification, detecting amplified products through polyacrylamide electrophoresis, and obtaining a DNA finger-print chromatogram; performing polymorphic analysis and J genetic similarity coefficient analysis on the saccharum arundinaceum resources, and constructing UPGMA phylogenetic trees; performing comprehensive analysis on different ecological positions, phenotypic variation characteristics and phylogenetic trees of different saccharum arundinaceum resources, so as to obtain the screened material. By adopting the technical scheme and in combination with the phenotypic character variation analysis and a molecular marker technology, screening results are more sensitive and accurate; the utilization potential of saccharum arundinaceum germplasm resources is comprehensively evaluated, the accharum arundinaceum breeding material is screened, and the method can serve cane breeding.

The invention provides SSR primers, applications of the SSR primers in detection of polymorphism of saccharum arundinaceum, a design method of the SSR primers, and a method used for detecting the polymorphism of saccharum arundinaceum by using the SSR primers. The detection method is capable of detecting some genomic SSR locus information of saccharum arundinaceum; in addition, according to verification of polymorphism of saccharum arundinaceum by using the designed primers, the designed primers can be used for the research on genetic diversity of saccharum arundinaceum and construction of genetic maps effectively; an advanced gene analyzer is used in the development of the polymorphism, so that accuracy of primer polymorphism detection is increased.

The invention relates to a method for identifying erianthus arundinaceus consanguinity in sugarcane by a sub telomere sequence. The method comprises the following steps: preparing a root-tip cell slide, preparing a label probe, pre-treating the root-tip slide, preparing a denatured hybrid mixture, and hybridizing the denatured hybrid mixture and the pretreated root-tip slide. If a green fluorescence signal is generated on the hybrid slide, judging that a to-be-detected sample comprises erianthus arundinaceus consanguinity which is a real filial generation of sugarcane and erianthus arundinaceus. A nucleotide sequence of the generated green fluorescence signal probe is a nucleotide sequence as shown in SEQ ID NO: 1 in a sequence table. The method integrates a fluorescent in-situ hybridization technique, utilizes a special sub telomere sequence probe, relative to the sugarcane, of erianthus arundinaceus to identify the real filial generation of sugarcane and erianthus arundinaceus, has the characteristics of being high in efficiency, good in sensitivity, good in stability and high in reliability, can detect reality of relatively high filial generation by only needing to obtain root-tips of sugarcane, and provides a stable and effective identifying method for hybrid utilization of erianthus arundinaceus in a sugarcane genetic breeding improvement process.

The invention relates to a method for identifying consanguinity of saccharum arundinaceum in sugarcane via a spread sequence. The method comprises steps of preparing a root-tip cell slide, preparing alabeled probe, pre-processing the root-tip slide, preparing a denaturing hybrid mixed solution and hybridizing the denaturing hybrid mixed solution and the pre-processed root-tip slide. A to-be-detected sample is judged that the sample contains the consanguinity of saccharum arundinaceum and is a real hybrid later generation of sugarcane and saccharum arundinaceum when a green fluorescence signalis generated on the hybrid slide. The nucleotide sequence of a probe which generates the green fluorescence signal is shown as SEQ ID NO:1 in a sequence list. The method provided by the invention, through the combination with fluorescence in-situ hybridization technology and by identifying the real hybrid later generation of sugarcane and saccharum arundinaceum through the spread sequence probe that saccharum arundinaceum is specific to sugarcane, has the characteristics of being high in efficiency, good in sensitivity, good in stability and high in reliability; and the reality of any hybridgenerations can be detected just by acquiring root tips of sugarcane seedlings, so that an efficient and stable identifying method is provided for hybridization utilization of the saccharum arundinaceum in a sugarcane genetic breeding improving process.

The present invention provides a kind of rapid propagation method of schizomaea, comprising step 1: explant selection and sterilization; step 2, sterilized seeds are inoculated on the callus induction medium, and each bottle is inoculated with 10 Block explants, adjust the pH of the medium to 5.8, place them in dark culture at 25°C, and start to form callus after 5 to 7 days of culture; step 3, under dark culture conditions, the cultured on the callus medium Embryogenic callus, after peeling off, transferred to the subculture medium for culture, the pH of the medium was adjusted to 5.8, the temperature was 25±1°C, and every 20d to 28d was a multiplication cycle; step 4: bud differentiation, step 5: rooting and Proliferation; Step six: transplanting. The method for propagating the grass grass provided by the invention has the advantages of simple sampling, high induction rate of the cultured callus, high differentiation rate, high value-added coefficient, good stability, and can obtain a large amount of robust test-tube plantlets in a relatively short period of time, and The method has strong operability, low production cost and high application value, and is very suitable for industrial production.