The invention relates to a method for picea balfouriana somatic
embryo generation and
plant regeneration, which comprises steps of collecting picea balfouriana
open pollination clonal cones, and separating immaturate zygotic embryos out for somatic embryogenetic culture, wherein the somatic embryogenetic culture comprises four stages, i.e. an embryogenetic
callus inducing stage, an embryogenetic
callus multiplicating stage, a somatic
embryo maturity stage and a somatic
embryo germination stage. Compared with the prior art, the method has the advantages that the
seedling stage is greatly shortened, and natural maturing rate, seed maturation rate, differentiation rate and
germination rate are all improved. The
seedling culture method having short period, high multiplying rate, closely linked cultivation steps and low cost is provided for popularizing and planting picea balfouriana in a large scale for
forestry production, is suitable for a rapid propagation technical
system of picea balfouriana
cell engineering and satisfies the requirements of markets on picea balfouriana.