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247results about How to "High differentiation rate" patented technology

Bletilla seed tissue culture propagation method

The invention discloses a bletilla seed tissue culture propagation method which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following six steps of seed introduction and sterilization, induction culture of protocorm, enrichment culture of protocorm, induction culture of multiple shoots, enrichment culture of multiple shoots, and rooting culture. The method has the beneficial effects that seedlings generated by adopting seed tissue culture is low in pollution rate, high in differentiation rate, stable in hereditary characters, and high in active substance content. According to the method disclosed by the invention, the tissue culture propagation period of bletilla is also greatly shortened, and the tissue culture period from seeding to rooting is less than four months, so that the production time is shortened, and the production cost is lowered.
Owner:重庆市秀山红星中药材开发有限公司

Culture method for inducing adipose tissue-derived stromal cells to differentiate to chondrocyte

The invention relates to a culture method for inducing adipose tissue-derived stromal cells to differentiate to chondrocyte, and aims to solve the problem that the prior art is low in differentiation rate. The culture method comprises the following steps: 1) performing separation of primary cells of adipose tissue-derived stromal cells; 2) performing amplification and passage of the adipose tissue-derived stromal cells; and 3) performing differentiation culture on P5-generation adipose-derived stem cells to chondrocyte, namely, adding the adipose tissue-derived stromal cells into a condition culture medium, namely, an adipose tissue-derived stromal cell chondrocyte differentiation culture medium, after P5 generation of passage, performing chondrocyte differentiation culture, replacing the medium of the cells every 3 days, observing the morphological change of the cells, and after 16 days of induction, performing Alcian blue dyeing, and identifying the chondrocyte differentiation situation of the adipose tissue-derived stromal cells. The result of Alcian blue dyeing identification shows that the chondrocyte can be formed, and the Alcian blue dyeing is positive. The culture method has the characteristics of being simple and feasible, short in induction time as the induction culture medium is a serum-free culture system, good in test repeatability and high in osteoblast differentiation rate.
Owner:中卫华医(北京)生物科技有限公司 +1

Special anti-cracking foliar fertilizer for jujube tree, and preparation method and application thereof

The invention discloses a special anti-cracking foliar fertilizer for a jujube tree, and a preparation method and an application thereof. The special anti-cracking foliar fertilizer for the jujube tree is composed of the following components in percentages by mass: 5% to 8% of potassium chloride, 7% to 15% of calcium chloride, 1% to 5% of borax, 1% to 5% of chelated magnesium, 1% to 3% of chelatediron, 0.5% to 1.5% of zinc sulfate, 0.2% to 0.6% of manganese sulfate and 3% to 4% of organosilicon, with the balance being water. The foliar fertilizer provided by the invention has low production cost, is safe and effective, and is beneficial for and harmless to human and livestock; mineral elements added in the foliar fertilizer are needed by animals, and can effectively prevent a variety of diseases like yellow-leaf disease and little-leaf disease caused by nutrient deficiency of a fruit tree; the disease resistance of a tree body is strengthened; the quality of a fruit is improved; the soluble sugar content of a fruit is improved by 14.3% to 43.5%, the weight of a single fruit is increased by 5% to 13.3%; the proportion of high-quality fruits is increased by 20.2% to 69.8%; the proportion of low-quality fruits is decreased by 28.9% to 76.9%; the fruit cracking rate of golden-silk jujube is significantly decreased by 30% to 45%; meanwhile, the contents of mineral elements in fruits and leaves are significantly improved; and the foliar fertilizer is free of any side effects, is safe and reliable, and is a green and environment-friendly product.
Owner:CHINA AGRI UNIV

Sexual propagation method of bletilla striata

The invention provides a sexual propagation method of bletilla striata. The sexual propagation method of bletilla striata particularly comprises the steps of processing of capsules, preparation of nutrients, inoculation and cultivation, and bottle out domestication, and is characterized in that in the step of processing of capsules, an alcohol lamp drying mode replaces a filter paper sucking dry mode in the prior art; in the step of preparation of nutrients, combination of banana slurry and humus is adopted; in the step of inoculation and cultivation, only inoculation, germination cultivation and strong seedling cultivation steps are included, the conventional enlargement cultivation process in an existing tissue culturing technology is omitted, and the process is simplified and the cost is lowered while the purposes are achieved; in the step of bottle out domestication, cultivation is performed through humus in a greenhouse, domestication is finished under the condition of controlling the relative humidity, temperature and shading rate, and qualified bletilla striata seedlings are obtained. Bletilla striata seeds are used for scale seedling culturing, the survival rate is high, manual scale propagation of bletilla striata is achieved, and the obtained bletilla striata seedlings are low in contamination rate, high in differentiation rate and stable in inheritable character.
Owner:湖北济世药业有限责任公司 +1

Method used for inducing differentiation of human multipotential stem cells into retinal progenitor cells

The invention belongs to the field of biomedicine, and specifically relates to a method used for inducing differentiation of human multipotential stem cells into retinal progenitor cells. According to the method, human embryonic stem cells or human induced multipotential stem cells are induced in a medium containing N2, and then are delivered into a retina induction medium containing neural basis culture medium, knockout serum replacement and nicotinamide so as to obtain the retinal progenitor cells. The method is capable of increasing differentiation rate of human multipotential stem cells into retinal progenitor cells; and the retinal progenitor cells obtained via differentiation can be used for transplantation therapy of irreversible degenerative diseases of retinal neurons, such as age-related macular degeneration and retinitis pigmentosa, and also can be used for iPS cells sourced from patients as cell models in drug screening and gene interference experiments. Compared with existing technology, small molecule compounds used in the method are less, so that influences caused by non-anthropogenic factors are avoided effectively, and yield of obtained optic cup-like cells is obviously higher than that of existing induced differentiation methods.
Owner:FUDAN UNIV

Method for picea balfouriana somatic embryo generation and plant regeneration

The invention relates to a method for picea balfouriana somatic embryo generation and plant regeneration, which comprises steps of collecting picea balfouriana open pollination clonal cones, and separating immaturate zygotic embryos out for somatic embryogenetic culture, wherein the somatic embryogenetic culture comprises four stages, i.e. an embryogenetic callus inducing stage, an embryogenetic callus multiplicating stage, a somatic embryo maturity stage and a somatic embryo germination stage. Compared with the prior art, the method has the advantages that the seedling stage is greatly shortened, and natural maturing rate, seed maturation rate, differentiation rate and germination rate are all improved. The seedling culture method having short period, high multiplying rate, closely linked cultivation steps and low cost is provided for popularizing and planting picea balfouriana in a large scale for forestry production, is suitable for a rapid propagation technical system of picea balfouriana cell engineering and satisfies the requirements of markets on picea balfouriana.
Owner:INST OF FORESTRY CHINESE ACAD OF FORESTRY

Bletilla lateral bud tissue culture propagation method

The invention relates to a bletilla lateral bud tissue culture propagation method, and belongs to the technical field of traditional Chinese medicine material plantation. The method comprises the following steps of introduction sterilization, bud sprouting culture, multiple shoot induction culture, multiplication culture and rooting culture. The lateral bud is adopted for tissue culture, so that the pollution rate of the obtained seedlings is low, the differentiation rate is high, inheritable character is stable, and the content of active substances is high; and meanwhile, the tissue culture propagation period of the bletilla is greatly shortened, the tissue culture period from the cutting of lateral bud to the rooting is less than 4 months, the production time is greatly shortened, and the production cost is reduced.
Owner:重庆市秀山红星中药材开发有限公司

Method for acquiring regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants

The invention relates to a method for obtaining regeneration seedlings of dove trees through tissue culture by taking dove tree leaves as explants. The method comprises the following steps: step of: (1) acquiring calluses of the dove tree leaves; (2) breeding the calluses; (3) inducing cluster buds; (4) breeding the cluster buds; and (5) inducing roots. According to the method, the environmental temperature in steps (1)-(5) are 22-26 DEG C, and the environmental humidity is preferably 60%-90%. According to the method, the differentiation rate of the calluses is higher and reaches more than 50%; the rooting percentage is 100%; and the survival rate of transplanting is more than 90%.
Owner:SICHUAN UNIV

Method for fast propagation of test tube arrowhead by tissue culture

The invention discloses a method for fast propagation of test tube arrowhead by tissue culture. The test tube arrowhead is formed by sterilizing treatment of an explant, stem tip differentiation, subculture multiplication and induction. According to the method disclosed by the invention, the propagation of the test tube arrowhead is carried by tissue culture, and thus high propagation coefficient and short propagation period are realized; in addition, the whole propagation process is carried out under manual control conditions without being influenced by an external environment; the test tube arrowhead can be annually produced; due to small size and light weight, the test tube arrowhead can solve the problems existing in the remote transportation and storage of arrowhead test tube seedlings; the test tube arrowhead has no disease, so that the problem that a conventional arrowhead seed easily carries diseases is solved; the induction of the test tube arrowhead provides an operable simple system for research work of the carmus expansion mechanism; as an in-vitro dormancy organ, the in-vitro indoor preservation research on arrowhead germ plasm resources can be realized; and live exchange of international germ plasm resources is benefited. The invention provides a quick, convenient and effective propagation method for the development of the arrowhead industry.
Owner:WUHAN VEGETABLE RES INST

Tissue-culture propagation method of cold-resistant Chinese rose

The invention relates to a tissue-culture propagation method of cold-resistant Chinese rose and solves the propagation problems of slow propagation and low multiplication coefficient of the cold-resistant Chinese rose. The method includes the steps of firstly, screening explants; secondly, sterilizing the explants; thirdly, performing primary culture; fourthly, performing multiplication culture; fifthly, performing strong-seedling culture; sixthly, performing rooting culture; seventhly, transplanting and performing acclimation. The method is characterized in that newly grown top buds or lateral buds of plants are used as the explants, MS is used as the basic culture medium, naphthylacetic acid (NAA), 6-benzylamino purine (6BA) and indolebutyric acid (IBA) are utilized to perform hormonal regulation, and accordingly the regeneration system of the cold-resistant Chinese rose is built. The method has the advantages that one multiplication subculture cycle of the method is 4-5 weeks, multiplication times is controlled to be 6-8 times, the method is unaffected by seasons, seedling propagation coefficient can be increased greatly, a scientific and effective propagation method is providedfor the cultivation and application of the cold-resistance Chinese rose, production requirements are satisfied, industrial propagation can be performed, and reference can be provided for the propagation of other cold-resistant Chinese rose varieties.
Owner:HEILONGJIANG ACAD OF SCI INST OF NATURAL RESOURCES +1
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