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Monkshood-tuber tissue culture and rapid propagation method

A technology for tissue culture and A. chinensis, applied in the field of tissue culture and rapid propagation of A. chinensis, can solve problems such as low yield, poor disease resistance, instability, etc., achieve high differentiation rate and rooting rate, solve poor disease resistance, and survival rate. high effect

Active Publication Date: 2010-12-29
雅安三九中药材科技产业化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main purpose of the present invention is to provide a method for tissue culture and rapid propagation of Aconite in order to solve the problems of poor disease resistance, low and unstable yield, and mixed varieties of current varieties in the production of Aconite. , laying the foundation for the production of Chuanfuzi GAP

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0030] In this embodiment, the shoot tip of the Aconite plant in Pingwu Aconite Provenance Base of Sichuan was selected as the explant, and the callus was induced by tissue culture for rapid propagation, including the following main steps:

[0031] Step 1: Selection and disinfection of explants

[0032] In the Sichuan Pingwu Fuzi Provenance Base, in March, the shoot tip of the annual robust Aconitum plant was selected as the explant (as the inoculation material), and treated at a low temperature of 4°C for 3 days; rinsed with tap water, and treated with 75% alcohol for 15-15 20s, soak in 0.1% mercury chloride solution for 10-12min, pour off the mercury solution, and wash with sterile water for 4-5 times; peel off the stem tip and cut it into 0.2mm length, and set aside;

[0033] The second step: inoculation and induction of callus

[0034] Use MS as the basic medium, add NAA 0.1mg / L+6-BA 1.0mg / L to the basic medium as the medium for inducing callus, and inoculate the explants...

Embodiment 2

[0046] In this embodiment, the leaves of Aconite fruticosa plants in Sichuan Pingwu Aconite Provenance Base are selected as explants, and rapid propagation is carried out through tissue culture, including the following main steps:

[0047] Step 1: Selection and disinfection of explants

[0048] In the Sichuan Pingwu Aconite Provenance Base, in March, the leaves of the annual robust Aconitum plant were selected as explants (as inoculation materials), treated at a low temperature of 4°C for 3 days; rinsed with tap water, and treated with 70% alcohol for 15-20s , soak in 0.1% mercuric acid solution for 10-12 minutes, pour off the mercuric solution, and wash it with sterile water for 4-5 times; cut the leaves into 1cm×1cm cubes and set aside;

[0049] Step 2: Induction of Callus

[0050] Use MS as the basic medium, add NAA 0.2mg / L+6-BA 4.0mg / L to the basic medium as the medium for inducing callus, and inoculate the explants treated in the first step above according to the convent...

Embodiment 3

[0062] In this embodiment, the stem section of the Aconite plant and the stem section with axillary buds in the Sichuan Pingwu Aconite Provenance Base are selected as explants, and the callus is induced by tissue culture for rapid propagation, including the following main steps:

[0063] Step 1: Selection and disinfection of explants

[0064] In the Sichuan Pingwu Fuzi Provenance Base, in March, the stem section of the annual strong Aconite plant and the stem section with axillary buds were selected as explants (as inoculation materials), treated at a low temperature of 4°C for 3 days; rinsed with tap water, Treat with 70% alcohol for 15-20 seconds, soak in 0.1% mercuric acid solution for 10-12 minutes, pour out the mercuric acid solution, and wash it with sterile water for 4-5 times; cut the stems and stems with axillary buds into about 1.5cm, stand-by;

[0065] The second step: inoculation and induction of callus

[0066] Use MS as the basic medium, add NAA 0.2mg / L+6-BA 2....

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Abstract

The invention discloses a monkshood-tuber tissue culture and rapid propagation method, which comprises the main steps of selection, disinfection and sterilization of an explant, inoculation and induction of callus, induction of cluster buds, root induction, hardening-seedling and transplanting and the like. By carrying out tissue culturing on stem tips, blades, roots, stem sections, leafstalks, seeds and the like of monkshood-tuber as the explants, and inducing a plant to regenerate and rapidly propagate monkshood-tuber seedlings, the inductivity, the differentiation rate and the rooting rate are higher, and the survival rate of the seedlings after being transplanted in culture soil is high. The method can effectively overcome the problems of poor disease resistance, low and unstable output, varietal complexity and the like of a traditional monkshood variety in the production of the monkshood-tuber, realizes the variety purification and rejuvenation through stem tip detoxification, tissue culture and other means, and provides excellent monkshood varieties, and lays a favorable foundation for the factory and scale production of large quantities of high-quality seedlings required by monkshood GAP (Good Agricultural Practices) bases and the like.

Description

technical field [0001] The invention belongs to the technical field of medicinal material cultivation, and in particular relates to a method for tissue culture and rapid propagation of aconite. Background technique [0002] Aconitum (Radix Aconiti Lateralis Preparata) is a processed product of the root of Aconitum carmichaeli Debx., a plant of the Ranunculaceae family. Mild, impotence, uterine cold, confidant cold pain, deficiency-cold vomiting and diarrhea, Yin-cold edema, Yang-deficiency exogenous pathogenesis, cold-damp arthralgia, genital sores, etc. [0003] Aconite is one of the traditional authentic Chinese medicinal materials in Sichuan, which has important medicinal and economic value. Jiangyou City, Sichuan Province, as the authentic production area of ​​aconite, has a planting history of more than 1,400 years. In 2006, Jiangyou Aconite obtained the protection of geographical indication products (Announcement No. 41 in 2006). It is a national aconite production co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 唐莉田孟良杨华吴锡明张小琴刘天成官华王巧胡丹胡莹莹
Owner 雅安三九中药材科技产业化有限公司
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