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Scientific and effective Sichuan radix aconiti lateralis preparata breeding method

A scientific technology of Radix Aconiti, which is applied in the field of propagation of Radix Aconitiae, can solve the problems of poor disease resistance, low yield, instability, etc., and achieve the effects of poor disease resistance, high differentiation rate, high rooting rate, and high survival rate

Inactive Publication Date: 2018-01-05
罗华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The main purpose of the present invention is to provide a method for tissue culture and rapid propagation of Aconite in order to solve the problems of poor disease resistance, low and unstable yield, and mixed varieties of current varieties in the production of Aconite. , laying the foundation for the production of Chuanfuzi GAP

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] In this embodiment, the shoot tip of the Aconite plant in Pingwu Aconite Provenance Base of Sichuan was selected as the explant, and the callus was induced by tissue culture for rapid propagation, including the following main steps:

[0031] Step 1: Selection and disinfection of explants

[0032] In the Sichuan Pingwu Fuzi Provenance Base, in March, the shoot tip of the annual robust Aconitum plant was selected as the explant (as the inoculation material), and treated at a low temperature of 4°C for 3 days; rinsed with tap water, and treated with 75% alcohol for 15-15 20s, soak in 0.1% mercury chloride solution for 10-12min, pour off the mercury solution, and wash with sterile water for 4-5 times; peel off the stem tip and cut it into 0.2mm length, and set aside;

[0033] The second step: inoculation and induction of callus

[0034] Use MS as the basic medium, add NAA 0.1mg / L+6-BA1.0mg / L to the basic medium as the medium for inducing callus, and inoculate the explants ...

Embodiment 2

[0043] In this embodiment, the leaves of Aconite fruticosa plants in Sichuan Pingwu Aconite Provenance Base are selected as explants, and rapid propagation is carried out through tissue culture, including the following main steps:

[0044] Step 1: Selection and disinfection of explants

[0045] In the Sichuan Pingwu Aconite Provenance Base, in March, the leaves of the annual robust Aconitum plant were selected as explants (as inoculation materials), treated at a low temperature of 4°C for 3 days; rinsed with tap water, and treated with 70% alcohol for 15-20s , soak in 0.1% mercuric acid solution for 10-12 minutes, pour off the mercuric solution, and wash it with sterile water for 4-5 times; cut the leaves into 1cm×1cm squares for later use;

[0046] Step 2: Induction of Callus

[0047] Use MS as the basic medium, add NAA0.2mg / L+6-BA4.0mg / L to the basic medium as the medium for inducing callus, and inoculate the explants treated in the first step above by conventional methods ...

Embodiment 3

[0056] In this embodiment, the stem section of the Aconite plant and the stem section with axillary buds in the Sichuan Pingwu Aconite Provenance Base are selected as explants, and the callus is induced by tissue culture for rapid propagation, including the following main steps:

[0057] Step 1: Selection and disinfection of explants

[0058] In the Sichuan Pingwu Fuzi Provenance Base, in March, the stem section of the annual strong Aconite plant and the stem section with axillary buds were selected as explants (as inoculation materials), treated at a low temperature of 4°C for 3 days; rinsed with tap water, Treat with 70% alcohol for 15-20 seconds, soak in 0.1% mercuric acid solution for 10-12 minutes, pour out the mercuric acid solution, and wash it with sterile water for 4-5 times; cut the stems and stems with axillary buds into about 1.5cm, stand-by;

[0059] The second step: inoculation and induction of callus

[0060] Use MS as the basic medium, add NAA0.2mg / L+6-BA2.0m...

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Abstract

The invention discloses a scientific and effective propagation method of Radix Aconiti Radix, comprising: selection and sterilization of explants, inoculation to induce callus, induction of clustered buds, induction of rooting, seedling hardening and transplanting, etc. Aconite shoot tips, leaves, roots, stem segments, petioles and seeds are used as explants for tissue culture to induce plant regeneration and rapidly propagate Aconite seedlings. The induction rate, differentiation rate and rooting rate are high, and they survive after being transplanted into the cultivation soil. High rate; it can effectively solve the problems of poor disease resistance, low and unstable yield, and mixed varieties of current varieties in the production of Aconite. Through the detoxification of stem tips and the use of tissue culture methods, the purification and rejuvenation of varieties can be achieved, and excellent varieties of Aconite can be provided. , Lay a good foundation for the industrialized and large-scale production of a large number of high-quality seedlings required by the Chuanfuzi GAP base.

Description

Technical field: [0001] The invention belongs to the technical field of medicinal material cultivation, in particular to a scientific and effective propagation method of aconite. Background technique: [0002] Aconite (Radix Aconiti Lateralis Preparata) is a processed product of the root of Aconitumcarmichaeli Debx., a plant of the Ranunculaceae family. , impotence, uterine cold, confidant cold pain, deficiency-cold vomiting and diarrhea, Yin-cold edema, Yang-deficiency exogenous pathogenesis, cold-damp arthralgia, genital sores, etc. [0003] Aconite is one of the traditional authentic Chinese medicinal materials in Sichuan, which has important medicinal and economic value. Jiangyou City, Sichuan Province, as the authentic production area of ​​aconite, has a planting history of more than 1,400 years. In 2006, Jiangyou Aconite obtained the protection of geographical indication products (Announcement No. 41 in 2006). It is a national aconite production commodity base, and it...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 罗华
Owner 罗华
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