701results about How to "High induction rate" patented technology

The invention relates to a forest tree seedling breeding technology and in particular relates to a method for suppressing browning of a carya cathayensis sarg explant. The method is characterized by selecting a carya cathayensis sarg stem with axillary buds as an explant in spring, disinfecting the explant, then inoculating the explant into a culture medium to which catechins and anti-browning agents are added, and firstly culturing a tissue culture vessel in the low temperature dark environment and then transferring the tissue culture vessel to the routine culture environment at the initial stage of inoculation. The method can conduce to obvious reduction of the browning rate of the carya cathayensis sarg explant and improvement of inductivity of the carya cathayensis sarg explant, is simple and convenient to operate, has a better browning suppression effect, and provides a technical support for tissue culture and asexual reproduction of carya cathayensis sarg.

The invention discloses a method using high-oil inducement series to induce the haploid and the polyembryony body of the corn. The method inducing the haploid and the polyembryony body of the corn provided by the invention is to use high-oil corn haploid inducement series as the father parent to pollinate the female parent, to obtain the first-generation grain and gain the haploid and the polyembryony body of the corn. The method not only has the advantages that the breeding process is simplified, the breeding course is accelerated, the purity of the breed is improved, etc. in the traditional haploid breeding; but also can use the genetic effect of the oil constituent to improve the identification efficiency of the haploid, and at the same can improve the inducement rate of the polyembryony body.

The invention discloses a method for building a high-efficiency regeneration system of superior corn self-bred line agriculture line 531, belonging to the field of plant genetic engineering and transgenosis breeding. The invention takes an agriculture line 531 rataria as an explant, induces in a callus induction medium and produces an II-type embryonic callus; the II-type embryonic callus is subjected to embryoid induction under light in an embryoid induction medium to produce a green embryoid; then, the green embryoid is transported to a regeneration medium and is cultured into a regeneration plant under light; root induction is carried out in a rooting medium, and acclimatization is carried out in Hogland nutrient solution to ensure that a new thick root grows on the root of the regeneration plant; the root is transplanted to nutritional soil for rejuvenation culture; and finally, the root is transplanted to a land for growing field crops to normally grow and seed. The regeneration technology is suitable for the superior corn self-bred line agriculture line 531 with high application value, can ensure that the superior quality of agriculture line 531 corn can be inherited in the corn transgenosis breeding process, and has an important meaning for functional genome group research.

The invention relates to a rapid propagation method for culturing Rieger begonias tissue, which comprises the following steps that: explant material is treated, that is, the explant material is selected for treatment to prepare the explant meeting the requirement; then inducement is carried out, that is, the explant prepared in the first step gets vaccinated to culture medium to induce bud on inducing conditions; the inducing condition that the light intensity with illuminance is less than 1500lx is met. As the lighting condition during the fast propagation of Rieger begonias tissue is further optimized, the application of the rapid propagation method for culturing Rieger begonias tissue causes the inducement time to be shorter and ensures the inducement rate to be higher and reach 100 percent.

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

The invention discloses a method for regeneration plant of tung oil tree leaf, and belongs to the technology field of rapid propagation of tung oil tree. The method comprises the steps of adventitious bud induction directly by tung oil tree leaf, subculture, multiplication culture, strong seedlings culture, rooting culture, acclimatization and transplantation, which not only provides a path for rapid breeding of excellent tung oil tree plants, and lays a solid foundation for improving resistance of the tung oil tree, tung oil tree output and properties of oil quality later on, and for building a tung oil tree genetic system.

The invention discloses a method for breeding chamomile. The method disclosed by the invention comprises the following steps: (1) inoculating chamomile seeds onto a seed germination culture medium to carry out sterile seedling cultivation so as to produce sterile seedlings; (2) taking hypocotyls of the sterile seedlings as explants, inoculating the explants onto a callus induction culture medium to carry out callus induction culture so as to generate calluses by virtue of induction; (3) inoculating the calluses generated by induction onto an adventitious bud differentiation culture medium to carry out adventitious bud induction differentiation culture so as to obtain adventitious buds; (4) inoculating the adventitious buds onto a rooting culture medium to carry out rooting culture and culturing adventitious roots by virtue of induction so as to obtain rooted seedlings; and (5) hardening and transplanting the rooted seedlings so as to finally obtain the chamomile. In a regeneration system established by the method disclosed by the invention, callus inductivity reaches up to 86.63%, differentiation rate of the adventitious buds reaches up to 25.5%, rooting rate reaches up to 100%, and transplanting survival rate reaches up to 100%, thus a large number of excellent chamomile test-tube plantlets can be obtained in short term so as to realize large-scale factory production.

The invention discloses a medlar sterile seedling and a method for obtaining the induced healing of the medlar sterile seedling, belonging to the technical field of plant tissue culture. The method comprises the following steps of drying fruits; disinfecting seeds: carrying out the seed disinfection by using sterile distilled water which contains 70 percent by weight of ethanol and 0.1 percent by weight of TritonX-100 (polyethylene glycol octylphenol ether); germinating seeds: firstly arranging the seeds into a refrigerator at 4 DEG C, standing for 2 days, and then removing the seeds to a culture room at the temperature of 25+ / -2 DEG C with the daily photoperiod of 14 hours to culture for 20+ / -2 days; healing subculture: arranging the seed on a subculture medium, culturing under at 25+ / -2 DEG C in the culture room with the daily photoperiod of 16 hours, and replacing the culture medium every two weeks. According to the method disclosed by the invention, both the medlar sterile seedling and the medlar induced callus are obtained, the step of carrying out physical trauma on the medlar callus in the inducing process of the medlar callus is simplified and the period of obtaining the medlar callus is shortened.

The invention discloses a method for inducing corn haploids. The method comprises the following steps: pollinating a female parent by a male parent corn haploid induction line with a higher corn haploid inductivity than a corn haploid induction line CAU5, and harvesting hybridized F1 generation grains to obtain corn haploids. A method for realizing the corn haploid induction line comprises the following steps: 1, introducing coding gene of a specific centromere histone mutant into a hybrid first generation plant of a corn inbred line HiIIA and a corn inbred line HiIIB to obtain a transgenic corn plant; and 2, hybridizing the specific histone mutant as a female parent with the CAU5 to obtain an F1 generation, hybridizing the F1 generation as a female parent with the CAU5 to obtain a BC1F1 generation, hybridizing the BC1F1 generation as a female parent with the CAU5 to obtain a BC2F1 generation, and continuously selfing the BC2F1 generation at least five generations to obtain the corn haploid induction line.

The invention relates to a method for establishing tissue culture regeneration system of tea tree. The method comprises the steps of inducing callus; performing callus subculture and proliferation; inducing adventitious bud; proliferating adventitious bud; hardening seedlings; rooting and transplantation. The method includes two proliferation stages, i.e., callus proliferation and adventitious bud proliferation. The callus proliferation manner has the characteristics of simple proliferation manner and stable inheritance. After subculture for 4 years, cell activity and differentiation ability have no obvious change. The callus with low phenols content can be taken as genetic transformation object. The technology establishes solid experimental foundation for establishment of genetic transformation system of tea tree.

The invention belongs to the field of genetic engineering of plants and discloses a method for building a high-efficiency regenerating and transforming system of Oryza sativaL. subsp. japonica 11 through cultivation of induction of embryogenic callus, subculture multiplication of embryogenic callus, transformation mediated by agrobacterium tumefaciens, cocultivation of embryogenic callus and agrobacterium tumefaciens, screening and cultivation of resistant callus and seedlings differentiation of resistant callus. The method has the advantages of simple technological processes, low production cost, and capabilities of obviously increasing the inductivity of the embryogenic callus, the subculture multiplication rate and the differentiation and regeneration rate of the embryogenic callus of the Oryza sativaL. subsp. japonica 11 and simultaneously shortening the period of obtaining regenerative rice plants; and on the basis, an improved agrobacterium tumefaciens-mediated method can be used for remarkably increasing the efficiency of obtaining the transgenosis rice plants and shortening the period of obtaining regenerative rice plants.

The invention discloses a tissue culture rapid propagation seedling method of hybrid paper mulberry, and belongs to the technical field of hybrid paper mulberry plantation. The tissue culture rapid propagation seedling method of hybrid paper mulberry comprises following steps: explant selection, induced culturing, subculturing, rooting culture, and transplanting. According to the tissue culture rapid propagation seedling method, current year stems with buds are selected as explants, the meristematic capacity is excellent, and differentiation propagation is more convenient to realize; LED illumination culture is capable of adjusting culture material growth process, increasing the environmental adaptability, realizing reasonable optimization on composition of induction medium, proliferationmedium, and rooting medium, and illumination conditions of different stages, increasing seedling tissue culture efficiency, and achieving relatively induction rate, reproduction rate, and rooting rate; the adventitious bud induction differentiation rate is 94% or higher; after subculture, average seedling height reaches 3.5cm, rooting rate reaches 100%, and transplanting survival rate reaches 68%or higher. The tissue culture rapid propagation seedling method is adopted for seedling of hybrid paper mulberry, the seedling efficiency is high, the propagation coefficient is large, seedling cost is reduced, and the obtained tissue culture seedlings are strong, and are high in quality.

The invention relates to a method for cultivating and producing polygalacic acid with polygala root hair-shaped roots, which uses polygala root hypocotyl and blades as an explantation and converts by activating hair-shaped root agrobacterium, and leads T-DNA of hair-shaped root agrobacterium Ri plasmid to be integrated to polygala root nucleus DNA for expressing and forming hair-shaped roots, and polygala root hair-shaped roots are gained through plural times of subinoculation. The polygala root hair-shaped roots which are gained contain the same components of polygalacic acid (namely, senega-saponin) as that of traditional Chinese medicine polygala root, and the content is not lower than the wild traditional Chinese medicine polygala root. The invention can be used as new medicine resources to provide polygala root products, which solves the practical problems that the demand amount of polygala root is increased year by year, and wild resources are lacked and the quality of cultivated breeds is reduced, which brings practical problems to clinic, Chinese patent drug processes and export.

The invention discloses a method for breeding a corn haploid induction line. An identification method for the colored embryo haploid of the induction line is developed so that haploid identification of the corn haploid induction line can be realized, thus doubling is conducted, an inducible line DH line with high inductivity, high identification efficiency and excellent agronomic traits can be screened out from the expression of four aspects of molecular marker-assisted selection inducible genes, inductivity, haploid identification efficiency and the agronomic traits, and the excellent haploidinducible line can be obtained within 1-2 years in the whole process. According to the method capable of rapidly breeding the corn haploid inducible line, the breeding process of the haploid inducible line is accelerated, a feasible scheme for solving the problems of long breeding period and large workload of the corn haploid induction line is provided, the breeding period of the haploid induction line can be greatly shortened, the workload of test of each generation is decreased, and the haploid breeding efficiency is improved, thus development of a corn haploid breeding technology is promoted, and the method has important significance.

The present invention discloses a tree peony callus induction method, applying MS culture medium that contains 6-BA, cytokinin, NAA plant growth regulator, 2, 4-D weedicide to induce callus. There are specific culture mediums and hormone combinations in allusion to different explants of tree peony seeds, stem, leaf, petal, anther etc. The invention also expatiates the optimal material drawn period of the callus. The inductivity of the tree peony callus are more than 80% and achieves a preferable induction impression; and the inductivity of the petal and the anther also reaches 50%.

The invention discloses a method for cultivating a corn haploid inducer with higher corn haploid inductivity than a corn haploid inducer CAU5. The method comprises the following steps: 1, inducing coding gene of a specific histone mutant into a hybrid first generation plant of a corn inbred line HiIIA and a corn inbred line HiIIB to obtain a transgenic corn plant containing the specific histone mutant; and 2, hybridizing the specific histone mutant as a female parent with the corn haploid inducer CAU5 to obtain an F1 generation, hybridizing the F1 generation as a female parent with the corn haploid inducer CAU5 to obtain a BC1F1 generation, hybridizing the BC1F1 generation as the corn haploid inducer CAU5 to obtain a BC2F1 generation, and continuously selfing the BC2F1 generation five generations to obtain the corn haploid inducer with higher corn haploid inductivity than the corn haploid inducer CAU5.

According to the invention, young leaves or stems of plants of Psammosilene tuniceoides W. C. Wu et C. Y. Wu are taken as an explant to successfully induce callus of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu, and a callus culture system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu under the conditions of light and dark cultivation is established to induce the differentiation of the callus to produce adventitious roots, thus establishing an adventitious root cultivation system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu. Moreover, the content of total saponins of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu is determined, and the conditions and parameters of plant cell culture are further optimized, thus establishing a high-yield cell culture system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu. Therefore, the plant cells of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu are cultured in a large scale to produce the adventitious roots which substitute for the original plant of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu to be used as medicine.

The invention discloses a tissue culture and rapid propagation method for actinidia chinensis, and relates to the tissue culture and rapid propagation method for actinidia chinensis seedlings. According to the method, young leaves of the current year serve as explants and are inoculated on a medium MS+0.3mg / LZT+0.05mg / LNAA subjected to callus induction and cluster bud differentiation. The invention also provides an improved MS; after cluster buds are differentiated, the cluster buds are sub-cultured in an improved MS+2mg / LZT proliferation culture medium, and are transferred into an improved MS+0.015mg / LTDZ thickening culture medium according to seedling height; small seedlings which are in accordance with rooting are transferred into 1 / 2 of improved MS+0.7mg / LIBA+0.1g / LAC rooting culture medium; the small seedlings are transplanted and are domesticated after roots grow. By the method, the callus induction rate reaches 96.4 percent; the height of cluster bud reaches 1.67cm; the proliferation coefficient of cluster buds reaches 3.67; the rooting rate reaches 98.12 percent; moreover, the culture medium is low in cost and short in propagation period, and industrial production can be realized.

The invention discloses a method for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds, relates to a method for inducing pine plant regeneration through in vitro culture of adventitious buds, and aims at solving the problems that in the existing tissue culture method of hybrid larch, organogenesis is difficult, plant regeneration rate is low, and adventitious bud induction rate is low. The method comprises the following steps: 1 inoculating pretreated zygotic embryos of the hybrid larch into a culture medium, and carrying out dark cultivation or light cultivation until callus tissue is generated; 2 inoculating the callus tissue into the culture medium, carrying out light cultivation until an adventitious buds grow; 3 cutting the callus tissue with the adventitious buds into blocks, transferring into the culture medium, carrying out subculture for once after 3 weeks, carrying out adventitious bud elongation, transferring into the culture medium, and carrying out stooling of the adventitious buds; and 4 transferring the stooled adventitious buds into the culture medium, and carrying out light cultivation to realize rooting of the adventitious buds. According to the method disclosed by the invention, the induction rate of the adventitious buds is 87.73%; the stooling rate is 75.96%; the rooting rate is 45%; and the method is applied for inducing hybrid larch plant regeneration through in vitro culture of adventitious buds.

The present invention discloses a method suitable for commercially inducing a corn haploid. The method comprises the following steps: carrying out hybridization of a corn haploid induction line I and a corn haploid induction line II to obtain a progeny recorded as an induction line hybrid, adopting the induction line hybrid as a male parent, carrying out hybridization of the induction line hybrid and a female parent material expected to obtain a haploid to harvest F0 hybridization kernel, and taking the haploid kernel to obtain the corn haploid, wherein the corn haploid induction line I and the corn haploid induction line II are different. Compared with the traditional method, the method of the present invention has the following characteristics that: on the basis of maintaining of a high induction rate, agronomy characteristics of the male parent (induction line hybridization combination) in the haploid induction is substantially increased, and especially a plant growth condition, a pollen disseminating amount, a pollen disseminating duration time, adaptability, resistance, and the like are substantially increased so as to improve haploid induction efficiency, provide a guarantee for efficient haploid induction, and provide possibility for large-scale haploid induction.

The invention relates to a tissue culture method for cultivating amaryllis vittata by utilizing bulbs. The tissue culture method comprises the following steps: selecting and disinfecting explants; performing induction culture; performing subculture multiplication culture; rooting; transplanting. According to the invention, small bulbs of amaryllis vittata are taken as explants, so that the reduction of contamination rate is benefited and the induction rate is increased; cluster buds can be induced without callus stage; the cluster buds can be directly induced so as to acquire bulbs; the induction time is shortened; the buds can be added for multiplication culture; the multiplied test bulbs are rooted; one-step bulb forming of amaryllis vittata bulbs can be realized according to the invention; the time of tissue culture is saved; the operation steps are reduced; the excellent natures of original bulbs are kept; an effective method is supplied for industrial efficient cultivation of amaryllis vittata; a technical support is supplied for the industrial development of amaryllis vittata.

The invention belongs to the technical field of biological engineering, and relates to a culture medium for the spore germination and seedling culturing of pteridophyte, which is characterized by consisting of the following nutritional components: 2 to 60 mmol.L<-1> nitrate ions, 1 to 30 mmol.L<-1> ammonium ions, 0.3 to 8 mmol.L<-1> phosphate radical ions, 2.7 to 66 mmol.L<-1> potassium ions, 0.37 to 12.0 mmol.L<-1> calcium ions, 0.18 to 1.8 mmol.L<-1> magnesium ions, 0.173 to 1.73 mmol.L<-1> sulfate ions, 10 to 100 mumol.L<-1> chelated ferric salt or chelated ferrous salt, 10 to 200 mumol.L<-1> boric acid, 10 to 100 mumol.L<-1> divalent manganese ions, 3 to 30 mumol.L<-1> zinc ions, 0.5 to 8 mumol.L<-1> divalent copper ions or complexes thereof, 0.1 to 1 mumol.L<-1> molybdate ions, 0.01 to 0.1 mumol.L<-1> divalent cobalt ions, 0.05 to 12 mumol.L<-1> chloride ions, 0.3 to 30 mumol.L<-1> thiamine hydrochloride and the balance of water, wherein the pH value of the culture medium is between 4.0 and 9.0. The culture medium can improve the germination rate of spores.

The invention discloses a propagation method for keeping a cabbage RGMS male sterile line, which comprises the following steps: selecting a scape leaf blade of the cabbage male sterile line, inoculating the scape leaf blade on an adventitious bud differentiation induction culture medium for cultivation, and when adventitious buds grow to a height of between 2.0 and 4.0 centimeters, transplanting the adventitious buds to an adventitious root differentiation induction culture medium for cultivation to obtain a complete regrowth seedling; transplanting the regrowth seedling to cultivating soil for cultivation; when the seedling grows to have 6 to 8 true leaves, hardening off the seedling adaptively for 3 to 5 days and then planting the seedling permanently in a field (an open ground), wherein the survival rate of the seedling is over 95 percent. By adopting the method, the breeding effect of the cabbage can be improved, and the normal breeding of the male sterility of the cabbage is ensured and enlarged. The method overcomes the complexity of the normal breeding of the male sterility of the cabbage, not only keeps the sterile line but also greatly improves the propagation coefficient, and provides guarantees for mass seed production.

The invention provides a method for in-vitro induction of a lobed kudzuvine root microtuber, and relates to the technical field of lobed kudzuvine root cultivation. The method comprises the followingsteps that 1) a rooting culture medium is inoculated with lobed kudzuvine root tissue culture seedlings for rooting culture so as to obtain rooted seedlings; and 2) a microtuber induction culture medium is inoculated with the rooted seedlings for induction culture so as to obtain the lobed kudzuvine root microtuber. The method has the advantages that the induction rate of the microtuber can be obviously increased, the induction time of the microtuber can be shortened, and the induction efficiency of the microtuber can reach 95% or above within the induction time of 20-25 days.

The invention discloses a method for breeding a corn inbred line by using the hybridizing induction of haploids, and belongs to the field of crop breeding methods. The method comprises the following steps: pollinating basic population or cenospecies according with breeding targets by using JASS31, and selecting aleurone layer top purple and purple-free embryonic tip labeled grains; naturally or artificially doubling the obtained material, and inbreding; and selecting non-separated head progeny rows realizing consistent progenies as a new inbred line. The method has a high haploid inductivity reaching above 13%, and has the advantages of obvious selection labeling, easy screening and high accuracy; and additionally, the JASS31 used in the invention has a moderate height, a long flowering time and a large pollen amount, and natural pollination induction of haploids can be carried out, so the artificial pollination can be avoided, labor saving is realized, and it is suitable for the large scale population operation. The method also has the characteristics of high breeding efficiency, simple operation and fast breeding speed.

The invention discloses a multiploid bulb induction method with a firtillaria cirrhosa scale leaf as an explant. The innovation of the invention lies in that a used material is a close callus with a certain differentiation characteristic, which is obtained through a predifferentiation culture process on the basis of generating a callus through induction of the scale leaf and has. The material is cut into small blocks and is soaked into a colchicine solution to carry out multiploid mutagenesis treatment, thereby greatly lightening damage of the colchicine solution to a processing material and enhancing the inductivity of the firtillaria cirrhosa multiploid bulb.

The invention provides a cultivation method for high yield and high quality industrial cannabis sativa. The method mainly includes the steps of: 1. flower bud collection and screening; 2. flower bud sterilization and inoculation; 3. anther induction culture; 4. callus redifferentiation; 5. adventitious bud proliferation and rooting; 6. pollen plant ploidy detection, and rejection of non-diploid plants; 7. transplantation of pollen plant seedlings; and 8. cross breeding, thus obtaining a holandric new line. The invention for the first time establishes a complete system for industrial cannabis sativa anther culture, also utilizes the method to produce super-male plants and female plants, and the plants are subjected to cross breeding to cultivate the holandric new line, thus significantly improving the yield and quality of cannabis sativa fiber, and providing a material basis for the development and utilization of cannabis sativa and cannabis sativa fiber.

The invention relates to a culture medium of an induced haplobiont for culturing eggplant anther and a method of the culture medium, and belongs to the technical field of plant biology. The method for cultivating the eggplant anther provided by the invention comprises the following steps: with the eggplant anther as a material, carrying out anther culture to induce callus; carrying out differentiation culture on the callus; carrying out rooting culture on differentiated seedlings; carrying out acclimatization and transplanting; and carrying out haplobiont doubling germination, so as to finish regeneration of an eggplant plant. The culture medium provided by the invention is low in cost and good in culture effect; the method for culturing the eggplant anther by virtue of the culture medium provided by the invention has the advantages of high callus induction rate, simple culture method and short culture period; the culture medium and the eggplant anther culture technology provided by the invention are applied to eggplant genetic breeding; the excellent genotype individual bodies of the eggplant are greatly enriched; the breeding period is obviously shortened; and a reliable technical support is provided for variety improvement of the eggplant.

The invention introduces an effective inductive cultivation method for an autotetraploid of paulownia catalpifolia by using leaves of a diplontic paulownia catalpifolia tissue culture seedling. The method comprises: pre-cultivating the leaves of paulownia catalpifolia for 6 days, wherein the concentration of added colchicine is 30 mg / L, the concentration of dimethyl sulfoxide is 2%, and the soaking time is 48 hours; performing shake cultivation, wherein the induction rate of the tetraploid paulownia catalpifolia reaches 14.5%; detecting that the chromosome number of root tip cells of variant plants of paulownia catalpifolia is 80 by virtue of a chromosome counting method, wherein the chromosome number is two times of that of the corresponding diplontic paulownia catalpifolia; and detecting that the unicellular DNA content of the tetraploid leaves of the paulownia catalpifolia are two times of those of the corresponding diplontic paulownia catalpifolia by using a flow cytometer, showing that a tetraploid paulownia catalpifolia plant is obtained. The cultivation method for inducing autotetraploid of paulownia catalpifolia by colchicine disclosed by the invention has the advantages of being wide in material drawing, simple and convenient to operate, low in cost, high in induction rate and the like, and is of certain guiding significance for woody plants, the tetraploid of which is not easily obtained.

The invention belongs to the field of plant tissue culture, and discloses an onion callus induction method and a special culture medium thereof. The onion callus induction method comprises the following step of: carrying out callus induction culture on an onion explant by using a B5 culture medium added with 2,4-D and 6-BA, wherein the sucrose concentration of B5 culture medium is 30g / L and the agar concentration of B5 culture medium is 8g / L, the pH value of culture medium is 5.8, the added 2,4-D concentration is 0.5-2.0mg / L, and the 6-BA concentration is 1.0-4.0mg / L. The selected onion explant has the advantages of simple and convenient disinfection and sterilization method, and low pollution rate after inoculation and high callus induction rate.