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Tissue cultivating fast reproducing method for pot culturing red palm seedling

A technology of tissue culture rapid propagation and anthurium, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of culture condition consumption, slow reproduction speed, high production cost, etc., achieve fast reproduction speed and improve efficiency and the effect of low profit and cost

Inactive Publication Date: 2005-06-08
广东省珠海市园艺研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, this process takes more than 6 months, and requires high production costs (including the cost of culture medium and the consumption of culture conditions, etc.)
So the existing potted anthurium seedling propagation technology still has the following defects: the propagation speed is slower and the cost is higher

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The potted anthurium seedling tissue culture rapid propagation method of the present invention comprises the following steps:

[0025] (1) Induction of callus

[0026] (1) Get the tender leaves of Anthurium fully expanded for 2 days as explant;

[0027] (2) the blade is first treated with 75% alcohol for 15 seconds, then processed in 0.1% mercuric acid solution for 7 minutes, taken out, rinsed with sterile water for 5 times, then immersed in sterile water for subsequent use, and the blade is immersed in sterile water, to prevent leaf browning;

[0028] (3) The blades treated with mercuric chloride were treated with BA 2.5mg / l and 2,4-D 2.0mg / l pretreatment liquid for 20 minutes;

[0029] (4) Cut the pretreated leaves into 0.6cm × 0.6cm blade cuts, put the blade cuts into sterile water, and put them into petri dishes in batches;

[0030] (5) Cut the leaves into pieces and inoculate them in batches on the induction medium for cultivation, keep the leaf surface of the c...

Embodiment 2

[0044] The potted anthurium seedling tissue culture rapid propagation method of the present invention comprises the following steps:

[0045] (1) Induction of callus

[0046] (1) Get the tender leaves of Anthurium fully expanded for 5 days as explants;

[0047] (2) The blade is first treated with 75% alcohol for 15 seconds, then taken out in 0.1% mercuric solution for 8 minutes, rinsed with sterile water for 5 times and immersed in sterile water for subsequent use;

[0048] (3) The blades treated with mercuric chloride were treated with BA 2.5mg / l and 2,4-D 2.0mg / l pretreatment liquid for 20 minutes;

[0049] (4) Cut the pretreated leaves into 0.6cm×0.6cm blade cuts, and put the blade cuts into sterile water for subsequent use;

[0050] (5) Cut the leaves into pieces and inoculate them in batches on the induction medium for cultivation, keep the leaf surface of the cut pieces of the leaves upward during inoculation, and the induction medium is added with 6-BA 1.0mg / l, 2.4-D...

Embodiment 3

[0064] The potted anthurium seedling tissue culture rapid propagation method of the present invention comprises the following steps:

[0065] (1) Induction of callus

[0066] (1) Get the tender leaves of Anthurium fully expanded for 4 days as explant;

[0067] (2) the blade is first treated with 75% alcohol for 15 seconds, then taken out in 0.1% mercuric solution for 7 minutes, rinsed with sterile water for 5 times and immersed in sterile water for subsequent use;

[0068] (3) The blades treated with mercuric chloride were treated with BA 2.5mg / l and 2,4-D 2.0mg / l pretreatment liquid for 20 minutes;

[0069] (4) Cut the pretreated leaves into 0.6cm×0.6cm blade cuts, and put the blade cuts into sterile water for subsequent use;

[0070] (5) Cut the leaves into pieces and inoculate them in batches on the induction medium for cultivation, keep the leaf surface of the cut pieces of the leaves upward during inoculation, and the induction medium is added with 6-BA 1.0mg / l, 2.4-D ...

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Abstract

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

Description

technical field [0001] The invention relates to a method for tissue culture and rapid propagation of potted anthurium seedlings. Background technique [0002] In the previous method of propagating by cutting cuttings, one anthurium can only reproduce 3 to 4 plants a year, because of its low reproduction coefficient, it is far from meeting the market demand. Therefore, the propagation method of potted anthurium adopts tissue culture method more at present, and it is a kind of utilizing the totipotency of plant cell, cultivates the small piece tissue of plant in vitro, carries out the industrialized seedling raising technology of plant clone rapid propagation, and specific process is: The young leaves of Anthurium anthurium are cut off, the callus is induced first, and then adventitious buds are differentiated, and then cultivated into strong seedlings that can be transplanted and domesticated. Generally, this process takes more than 6 months, and req...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 文方德金剑平刘群良李华养徐红
Owner 广东省珠海市园艺研究所
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