71 results about "Anthurium" patented technology

The invention relates to the technical field of substrates, in particular to Anthurium substrate. The Anthurium substrate comprises, by weight percent, 45-50% of peat soil, 49.5-55% of urban organic waste, 0.2-0.6% of perlite, and 0.005-0.02% of bactericide. The urban organic waste is mixture of domestic sludge, mushroom residue, and compost of dry branches and fallen leaves. The bactericide is sodium p(dimethylamino)benzenediazo sulfonate. The components add up to 100% by weight. Cost in planting Anthurium can be effectively reduced by using the Anthurium substrate, water and fertilizer retention is improved, quality of Anthurium is improved, and disease and insect resistance of Anthurium is improved.

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

The invention relates to a tissue culture method of anthurium. The method comprises the steps of (1) inducing callus; (2) differentiating adventitious buds; and (3) carrying out subculture proliferating on adventitious buds, and transplanting and nursing. The method is characterized in that when seedlings in step (3) in a regrowth block mass grow to 2cm or more and respectively have 1 to 3 roots, the regrowth block mass can be used as a subsequent transplanting regrowth block mass; the method also comprises a step (4) of transferring the regrowth block mass into a greenhouse to carry out seedling hardening two weeks before the transplanting, and opening a tissue culture bottle cover 3 to 5 days before the transplanting; a step (5) of cutting the large block mass into small block masses, cutting off test-tube seedlings with roots, which is more than 3cm high, and immersing the test-tube seedlings in a chlorothalonil liquid medicine for 30 minutes; step (6) of adopting peat soil or peat soil, perlite and river sand as cultivation substrates, transplanting the regrowth block mass and the test-tube seedlings, wherein the substrate is 1 to 1.5cm away from a seedling sieve notch, the small block mass is used as a transplanting unit, and 2 to 3 test-tube seedlings are used as a transplanting unit; and step (7) of nursing the seedlings after the transplanting. By adopting the method, the environmental applicability of the test-tube seedlings can be improved, and the survival rate can be increased by 8 percent.

The invention discloses a method for obtaining anthurium haplobionts and belongs to the technical field of genetic breeding of flowers and plants. The method includes the steps that (1) culture mediums are prepared; (2) inflorescences are selected, pretreated at low temperature and disinfected; (3) callus is induced; (4) proliferation culture is performed; (5) differentiation culture is performed; (6) rooting culture is performed; (7) seedlings are transplanted and hardened; (8) chromosomes are identified; (9) haploid propagation is performed. After the anthurium inflorescences are pretreated at the low temperature from 5 DEG C to 8 DEG C for 24 h to 72 h, the acquisition rate of sterile anthers and the induction rate of effective callus can be remarkably increased, and thus anthurium haplobionts can be effectively obtained; the operation method is easy and convenient to perform, the anthurium haplobionts can be used for further breeding a new variety of anthurium as the breeding material, and the breeding cycle is shortened.

The invention discloses greenhouse cultivation techniques of anthurium. Through techniques of facility construction, planting and culturing medium, plant shifting, water and fertilize management, temperature and light management, plant pruning, pest and disease controlling, and collecting and the like, the anthurium is enabled to be good in growing quality, bright in flower color, and easy to sell, and economical benefits of the anthurium are improved.

The invention belongs to the field of molecular breeding and discloses a genetic transformation method for transforming anthurium with DREB gene. The method is to incise the aseptic callus of Anthurium anthurium, infect with the Agrobacterium liquid containing the recombinant plasmid pCAMBIA1301-DREBa for 10 minutes, co-cultivate for 3 days, perform antibacterial culture for 5 days, and screen to obtain hygromycin-resistant rooted shoots. . It was confirmed by PCR detection that the exogenous gene had been transferred into the genome DNA of Anthurium anthurium. The present invention establishes its genetic transformation system for Anthurium for the first time, provides theoretical basis and experimental basis for Anthurium molecular breeding, and will effectively promote its molecular breeding process.

The invention discloses an anthurium tissue culture and fast propagation seed production method, which comprises the following steps: S1, explant sterilizing; S2, callus induction culture; S3, callus propagation culture; S4, callus differentiation culture; S5, successive transfer culture; S7, plantlet hardening; S8, transplanting. The invention provides an anthurium tissue culture and fast propagation culture medium, which comprises a callus induction culture medium, a callus propagation culture medium, a callus differentiation culture medium, a successive transfer culture medium and a rooting culture medium. According to the anthurium tissue culture and fast propagation culture medium and the anthurium tissue culture and fast propagation seed production method provided by the invention, the success rate of anthurium seed production is improved, the seed production period of anthurium tissue culture seedlings is shortened, and the aberration rate of callus differentiation is reduced. The yield and the quality of the anthurium tissue culture seedlings are improved; by utilizing a sterilization method, the pollution rate is effectively reduced; by utilizing a culture medium formula, the callus induction rate is high, the callus differentiation variation is low, the culture period is shortened, the probability of variation seedlings during successive transfer culture is reduced, the generation number of successive transfer culture is prolonged, and the yield is improved.

The invention discloses an anthurium soilless cultivation method. The method includes the steps of cultivating young anthurium seedlings in a soilless cultivation matrix, and transplanting the seedlings to be cultivated in a hydropinics nutrient solution after the seedlings root so that the seedlings can rapidly root, wherein the root systems of cultivated plants can be stronger and the vitality can be higher. The ingredients of the nutrient solution can be changed according to the different requirements for nutrients in different growth periods, the waste of the nutrients is reduced, the plant growth is more vigorous, and the ornamental value is higher.

The invention discloses an anthurium rapid propagation method. According to the method, anthurium ramets are selected, and are soaked for 10-30min in an antioxidant with a mass percentage of 1-5%; after the sterilization disinfection treatment, the ramets are inoculated into an induction medium for culturing; dark culture is carried out for 5-10 days, and light culture is carried out, such that anthurium callus are obtained; the callus are transferred to a proliferation medium, and are cultured for 20-25 days with an illumination time of 10-12h / d, an illumination intensity of 3000-3500 lx and a culture temperature of 22-28 DEG C, such that buds are differentiated; when the differentiated buds are 1-2cm, the buds are transferred into a rooting medium, and are cultured for 20-30 days with an illumination time of 5-8h / d, an illumination intensity of 1500-2000 lx and a culture temperature of 22-28 DEG C, such that rooted anthurium is obtained. With the method, a large amount of anthurium with good genetic stability can be obtained rapidly; a tissue culture cycle is shortened; anthurium differentiation rate and rooting rate are greatly improved, and anthurium tissue culture cost is saved.

The invention discloses an anthurium myb transcription factor AaMYB1 and a carrier, engineering bacterium and application thereof. The complete prediction amino acid sequence of the transcription factor AaMYB1 is shown as SEQ ID NO:1, and encoding genes are shown as SEQ ID NO: 2. The transcription factor synthesized by MYB regulating anthocyanin is cloned from anthurium for the first time, and the ability of a target plant to synthesize the anthocyanin is improved by means of over-expression.

The invention relates to a cold-resistant anthurium induction culturing method which comprises the following steps of: selecting tender shoots of a main-bred anthurium variety as explants; inoculatingthe tender shoots into an induction culture medium to form calli; selecting the calli which are formed for 2 weeks after the induction; transferring the calli into a co-culture medium for 4-week culturing; selecting newly-generated calli; transferring the newly-generated calli into a subculture medium for culturing till a fast proliferation period; carrying out 2-week selection by exposing the calli to such conditions as a temperature of 5 DEG C and 2000Lux illumination; selecting and transferring the survived calli into the subculture medium for 4-week culturing; carrying out 2-week selection by exposing the calli to such conditions as a temperature of 0 DEG C and 2000Lux illumination; selecting and transferring the survived calli into a differentiation culture medium for differentiationand seedling formation; selecting and transferring the differentiated seedlings into a rooting culture medium so that the differentiated seedlings can grow into normal seedlings; continuing to grow the selected anthurium plants; continuing the growth and assessing and verifying the expression stability of the cold resistance; carrying out proliferation on the stable and fine plants; and culturingthe transgenic cold-resistant anthurium which resists the cold damage at low temperatures.

The invention relates to a cold-resistant anthurium transgenic culturing method which comprises the following steps of: selecting apical buds of a main-bred anthurium variety as explants; inducing calli; subculturing the calli till a fast proliferation period; carrying out 4 turns of selection by transferring the calli into a selection culture medium; selecting the resistant calli for induction and differentiation so as to obtain seedlings; carrying out histochemical staining and molecular detection on resistant plants; identifying the differentiated seedlings containing target genes; transferring the differentiated seedlings into a rooting culture medium so that the differentiated seedlings can grow into normal seedlings; determining cold resistance of selected transgenic plants; remaining the transgenic plants which are free from cold damage and grow well; continuing to grow the selected transgenic anthurium plants; assessing and verifying the expression stability of the cold resistance; carrying out proliferation on the stable and fine plants; and culturing the transgenic cold-resistant anthurium which resists the cold damage at low temperatures.

The invention discloses nutrient fluid for anthurium hybrida, which belongs to the field of nutrient fluid for plant cultivation. The nutrient fluid for the anthurium hybrida comprises the following compositions in millimole ratio (m mol.L<-1>): 7.32 to 8.51 m mol.L<-1> of N, 0.98 to1.47 m mol.L<-1> of P, 4.44 to 5.05 m mol.L<-1> of K, 0.80 to 4.50 m mol.L<-1> of Ca, 0.81 to 1.50 m mol.L<-1> of Mg, 1.46 to 1.61 of m mol.L<-1> S, 14.50 to 15.04 m mol.L<-1> of Fe, 9.00 to 20.05 m mol.L<-1> of B, 3.00 to 3.12 m mol.L<-1> of Zn, 2.96 to 3.00 m mol.L<-1> of Mn, 0.482 to 0.76 m mol.L<-1> of Cu and 0.50 to 0.78 m mol.L<-1> of Mo. During use, the nutrient fluid uses pure grass peat as cultivation substrate, adopts soilless cultivation to produce pot flower of the anthurium hybrida in order that the cultivated finished flower of the anthurium hybrida has the advantages of large plantlets, early blossoming, short cultivation period, good quality in appearance, lustrousness in leaves and pure color in flowers.

The invention discloses a method for cultivating potted anthurium under the condition of the subtropical zone dry-hot valley climate. By means of the method, temperature reduction and humidification are carried out at the same time. The potted anthurium is cultivated in a greenhouse. The environment parameter in the greenhouse is controlled in the mode of illumination control and temperature reduction and humidification control, wherein according to the illumination control, when illumination intensity exceeds 18000Lux, a black sunshade net at the outer layer of the greenhouse is unfolded for shading, and if the illumination intensity still exceeds 18000Lux, a silver sunshade net at the inner layer of the greenhouse is started for supplementing shading and meeting the illumination requirement; according to the temperature reduction and humidification control, when the indoor temperature in the day is higher than 27 DEG C, a water screen draught fan system is adopted for temperature reduction and humidification, namely a water pump is utilized for supplying water to a water screen in the greenhouse, an axial flow draught fan drives air to enter the greenhouse and pass through the water curtain, heat exchange is carried out between the air entering the greenhouse and water, and the humidification of the air entering the greenhouse increases, and when the indoor day and night temperature is below 17 DEG C, a hot-water pipeline laid at the bottom of a seedbed emits heat for increasing the temperature.

The invention belongs to the field of floriculture methods. An anthurium bacterial blight control method is characterized in that chemical is sprayed for the first time when waterlogging spots appear on the leaves; the chemical is sprayed for three times, once every seven days; the sprayed chemical liquid can be any of the following chemicals: 300-500 times-diluted bismerthlazol, or 2000-4000 times-diluted agricultural streptomycin, or 100-250 times-diluted chloramphenicol, or 1000-4000 times-diluted newly planted adriamycin, or 50-100 times-diluted zhongshengmycin. The anthurium bacterial blight control method has the advantages that the application of the method can prevent and cure more than 80% of anthurium bacterial blight.

The invention provides a cultivation method for inhibiting lateral buds of potted anthurium flowers from growing over-densely. Auxin with 10E-8 moles of concentration is sprayed on the growing points of five-month-old anthurium seedlings. By means of the cultivation method, the lateral buds of the potted anthurium flowers are inhibited from growing over-densely, the quality of the anthurium flowers is improved, and the potted anthurium flowers are full in plant shape and are sufficient in number, so that the sale standard and date are reached, and the shipment rate is ensured.

The invention relates to anthurium SSR (Simple Sequence Repeat) primer pairs and a kit based on transcriptome sequencing development. The invention provides sequences of 22 anthurium SSR primer pairs and a kit for identifying genetic relationship and hereditary properties of anthurium. The 22 primer pairs have polymorphism in 50 anthurium species, and match the hereditary diversity analysis on the 50 anthurium species with common knowledge of plant classification. The 22 primer pairs can be directly applied to more anthurium plant species to perform hereditary diversity analysis on germ plasm resources.

A fast reproduction method for Anzu flower includes taking the tissue of Anzu flower, using it as explant, culturing it in culture medium while controlling the development of calli, using the calli to directly induce globular embryo cluster, and using the globular embryo cluster for fast reproduction.

The invention relates to an anthurium culture medium and a method by adopting the medium to culture anthurium. The invention provides the anthurium culture medium, which is prepared by pinstrup turfy soil, perlite and coco coir in mass ratio of 1:0.3-0.5:0.3-0.5. The mass ratio of the pinstrup turfy soil, the perlite and the coco coir is 1:0.3:0.3. The total porosity of the anthurium culture medium is obviously improved by 54 percent and 56 percent, the problem that the root system of the anthurium is thin due to salinity accumulation is solved, the breathability is good, the air exchange growth of the root system is favored, gases such as carbon dioxide are fully exchanged, and the problems that clayed soil is easy to harden after being watered and air cannot be exchanged are solved. Through cultivation experimental management for a while, the root system is found to be thick with vigorous root hairs, and the whole plant grows well.

The invention discloses a composite fertilizer for potted anthurium. The composite fertilizer is prepared from the following raw materials in parts by weight: sheep manure, mushroom residue, bone meal, durian shells, wormcast, egg shells, traditional Chinese medicine additives and micro element fertilizers; through main ingredient secondary fermentation, after vermiculites absorb trace elements, the wormcast is added for uniform mixing; then through pelletizing and drying, the composite fertilizer is obtained. The method provided by the invention has the advantages that simplicity is realized; the mastering is easy; the fermented main ingredients do not have pathogenic bacteria and germs; the fertility is sufficient; trace elements and traditional Chinese medicine additives absorbed by contained vermiculite are slowly released; the fertility is durable; pests in the soil are killed; the breeding of the pathogenic bacteria and germs can be inhibited; meanwhile, the soil structure is improved; the soil hardening is avoided; the growth environment of the anthurium is improved; the sufficient nutrients are provided for the anthurium; the disease-resistant performance is enhanced; the anthurium is promoted to grow strong branches and exuberant leaves; the harm of soil insects such as aphids and bacteroidal diseases to the anthurium plants can be prevented; meanwhile, affiliated biogas is generated to be used, so that the production cost is reduced.

The invention discloses an anthurium plantlet culturing method, belonging to the technical field of plant culture and aiming to overcome the defects of the conventional anthurium culturing technology. The anthurium plantlet culturing method is characterized by comprising the following steps: culturing 1-8-week anthurium plantlets into flowerpots with the flowerpot diameter of 9 cm and the flowerpot spacing of 3.5 cm, namely keeping the plantlet spacing at 12.5 cm; increasing the plantlet spacing into 15 cm within the 8th-9th week; and after the 10th week, transplanting the anthurium plantlets into flowerpots with the flowerpot diameter of 17 cm. The anthurium plantlet culturing method has the advantages as follows: the survival rate of the anthurium plantlets obtained after tissue culture propagation is increased to more than 95%, and the growth vigor of the anthurium plantlets is good and integrally uniform; and under the condition that the growth vigor of the anthurium plantlets is not affected, the space on a seedbed is saved to the maximum extent.

The invention discloses a coated release-controlled fertilizer of pot-cultured anthurium and a preparation method thereof. On the basis of pure nitrogen, the specially-use fertilizer is composed of an L-shaped release-controlled composite fertilizer accounting for 65-85% and an S-shaped release-controlled composite fertilizer accounting for 15-35%. A polymer coating layer of the L-shaped release-controlled composite fertilizer includes polyethylene, regenerated polypropylene, talcum powder and a multiple fertilizer. The polymer coating layer of the S-shaped release-controlled composite fertilizer includes polyethylene, regenerated polypropylene, talcum powder, corn starch, calcium powder and a multiple fertilizer. The preparation method of the release-controlled specially-use fertilizer includes the steps of spraying the components of the polymer coating layers in the L-shaped release-controlled composite fertilizer and the S-shaped release-controlled composite fertilizer onto surfaces of composite fertilizer granules and then mixing the components. The release-controlled fertilizer is effectively reduced in fertilization times, saves labor intensity, reduces the content of nitrogen, phosphorus and potassium and such nutrients in leachate, is reduced in fertilizer waste, is reduced in pollution risk of nutrient leaching on surrounding river and underground water, and has a high application value on fertilization of the pot-cultured anthurium.

The invention discloses an pesticide for soybeans and a preparation method of the pesticide. The pesticide is prepared from, by weight, 10-20 parts of solanum pseudocapsicum, 20-30 parts of anthurium, 10-20 parts of cordate telosma, 5-15 parts of poinsettia, 10-15 parts of lycoris radiata and 5-20 parts of nandina. The pesticide is simple in formula, convenient to prepare, good in pest killing effect, free of harm to human and livestock, free of pollution after being used, free of influences on the environment and capable of being naturally degraded.

A tissue culture method for quickly reproducing the red cactus includes such steps as disinfecting the tender leaf of red cactus, pretreating in the pretreating liquid containing BA and 2,4-D, cutting to become blocks, inductive culture in the improved MS culture medium to induce calli, differentiating adventitious buds in the improved diferential culture medium, culturing the rooted aseptic seedlings in the improved reproductive culture medium, naturalizing, and transplanting them in pots.

The invention provides a hybridization breeding and rapid propagation method of anthurium. The hybridization breeding and rapid propagation method sequentially comprises the following six steps: material selection, manual pollination, sterile seed sowing and culture, propagation culture, seedling strengthening and rooting culture and test-tube plantlet transplanting. With the adoption of the method provided by the invention, the hybridization breeding success rate of the anthurium can be improved, certain asynchronous flowering period problems of hybridized combination are solved and high-quality germchits are rapidly cultured; and the hybridization breeding and rapid propagation method has the advantages of less investment, high yield and like, and is applied to large-scale production and protection of germplasm resources of the anthurium.

The invention relates to a method of increasing the coldness tolerance of red palm using NO, and in particular a method of increasing the coldness tolerance of red palm applying external source nitric oxide. At the initial stage of red palm budding, the date is normally selected between September and October, after the dew dries on the selected sunny day or cloudy day morning, the leaves are sprayed with the SNP solution at 8-9 o'clock, the spraying continues until the solution drips from the red palm leaves, the spraying is applied once every 3 days, and continuously for 3 times. When the method is adopted, after the red palms are transplanted and the red palms survive normally until the initial stage of buds emerging, the SNP spraying treatments on leaf surfaces are conducted, the coldness tolerance of red palm can be significantly increased, and the method has the advantages of being the simple in process and easy to operate, free of secondary pollution and low in cost, and large area treatments are facilitated.

The invention relates to an anthurium adndraeanum pollen culture method. The method comprises the following steps: an anther at a mid-late uninucleate stage is adopted and is inoculated to a callus induction medium, and callus is obtained by culturing; the callus is inoculated to a differentiation medium, such that test tube seedlings are obtained; the test tube seedlings are inoculated to a rooting medium, and screening is carried out, such that single plants are obtained. With the above culturing manner, an anthurium adndraeanum pollen callus induction rate reaches 88%, a rooting rate reaches 94%, and a transplanting survival rate reaches 90%.

The invention belongs to the agriculture flower technology field, and discloses a plantation method for promoting accumulation of Anthurium nutrient growth and prolonging flower viewing period, and the method comprises the steps of cultureing a sprout or cutting and breeding a sprout, then transplanting the sprout to an aperture disk for cultivation; after the aperture disk sprout is cultivated, transplanting the sprout to a double color basin to promote the plant and the root grow; when the root grows all over the double color basin soil, transplanting the sprout to another double color basin to cultivate a bed reflective membrane so as to promote the nutrition accumulation of the plant's stem and leaves; transplanting the plant whose root grows all over the double color basin to another double color basin for colonization so as to improve the sprout bed reflective membrane and promote the plant's growth and cultivation; taking the plant that has a good shape out for selling; when the Anthurium is ready for selling, putting the Anthurium for viewing. According to the invention, the growth of the Anthurium can be promoted obviously; the accumulation of nutrient growth can be improved; the flower viewing period can be prolonged; photosynthetic efficiency of the Anthurium plant can be obviously improved; the accumulation of nutrient growth can be increased; and bennet coarseness and Spathe diameter can be increased.

The invention discloses a method for increasing the germination rate of anthurium seeds by pretreatment before seed sowing. The method comprises the following steps: collecting rape fruits of anthurium, removing peels, then using water to clean mucilage on the seeds, sowing the seeds with mucilage being cleaned off in a matrix, and germinating and growing the seeds. The method is simple and efficient, the cost is low, the seeds are clean, and after the seeds are sown, germs are not easily bred to influence germination and growth; the method is adopted for directly sowing the anthurium seeds inthe matrix, the germination rate of manure fruit seeds is up to 97.00%; the method has the advantages of high germination rate and high seedling survival rate and the like and is beneficial to cultivating strong seedlings, shorting the breeding period and saving the breeding cost and the like.

The invention provides a prevention method for greenhouse anthurium thrips. A mixture of 3000 parts of spinetoram solution and 3500 parts of abamectin solution is sprayed and applied. The method is capable of stopping the extension of the thrips by spraying on flowers without damaging the growth of the plant body, and reducing the yield cost of the flowers and plants, so that the method has the superiority.