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Cold-resistant anthurium induction culturing method

A cultivation method and technology for inducing culture medium, which are applied in the field of induction cultivation of cold-resistant anthurium, can solve problems such as increasing production cost, reducing ornamental and commercial quality of anthurium, and achieve the improvement of quality, ornamental and commercial quality, and reduction of production cost. Effect

Inactive Publication Date: 2010-02-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, due to the low temperature in winter in our country, it is difficult to plant anthurium in the natural environment of anthurium in our country, and it is necessary to control the planting under artificial greenhouse conditions, which not only greatly increases the production cost, but also significantly reduces the ornamental value of anthurium and commercial quality

Method used

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Examples

Experimental program
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Embodiment 1

[0027] The shoots of the anthurium variety "Vitara" were used as explants, inoculated into the induction medium, and the callus was induced at 25°C; the callus that had been induced for 2 weeks was selected and transferred to the culture medium containing ABA 5mg / L The co-culture medium was co-cultured for 4 weeks; the new callus was picked, transferred to the subculture medium, and subcultured until the callus entered the rapid proliferation phase; the callus in the rapid proliferation phase was selected and placed in Screening for 2 weeks at a temperature of 5°C and a light of 2000 Lux; select the still surviving callus, transfer to the subculture medium, and after subculture for 4 weeks, move to a temperature of 0°C and a light of 2000 Lux for screening for 2 weeks; select the still surviving callus The callus was transferred to the differentiation medium to differentiate into seedlings; 21 differentiated seedlings were selected and transferred to the rooting medium to grow ...

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Abstract

The invention relates to a cold-resistant anthurium induction culturing method which comprises the following steps of: selecting tender shoots of a main-bred anthurium variety as explants; inoculatingthe tender shoots into an induction culture medium to form calli; selecting the calli which are formed for 2 weeks after the induction; transferring the calli into a co-culture medium for 4-week culturing; selecting newly-generated calli; transferring the newly-generated calli into a subculture medium for culturing till a fast proliferation period; carrying out 2-week selection by exposing the calli to such conditions as a temperature of 5 DEG C and 2000Lux illumination; selecting and transferring the survived calli into the subculture medium for 4-week culturing; carrying out 2-week selection by exposing the calli to such conditions as a temperature of 0 DEG C and 2000Lux illumination; selecting and transferring the survived calli into a differentiation culture medium for differentiationand seedling formation; selecting and transferring the differentiated seedlings into a rooting culture medium so that the differentiated seedlings can grow into normal seedlings; continuing to grow the selected anthurium plants; continuing the growth and assessing and verifying the expression stability of the cold resistance; carrying out proliferation on the stable and fine plants; and culturingthe transgenic cold-resistant anthurium which resists the cold damage at low temperatures.

Description

technical field [0001] The invention relates to a method for inducing and cultivating cold-tolerant anthurium. Background technique [0002] Anthurium andraenum, also known as Anthurium and Anthurium, is a perennial herb of the Araceae Anthurium family. It is listed as the main cut flower species in many tropical countries and regions, and is the second largest tropical flower commodity after Tropical Orchid. In the Dutch flower auction market in 2002, Anthurium was not only one of the 10 most popular cut flowers, but also one of the 10 most popular potted plants. The fastest growing species; potted anthurium sales amounted to 34.1 million euros, second only to phalaenopsis. [0003] The Netherlands, Israel, and Hawaii of the United States are currently the main cultivation centers of anthurium, especially the Netherlands is in the leading position in anthurium breeding and standardized cultivation. my country's anthurium industry has experienced more than 20 years of deve...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 田丹青舒小丽葛亚英叶红霞周志丹吴殿星
Owner ZHEJIANG UNIV
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