239 results about "Phalaenopsis" patented technology

The present invention relates to the quick breeding method of high quality seedling of Phalaenopsis, known as 'queen orchid' and the culture medium therewith. The present invention uses the flower stem section of Phalaenopsis as explant and induces flower stem seedling with unique culture medium and performs proliferation with fascicular bud stem tip or stem section to produce high quality seedling of Phalaenopsis. The method is simple, practical, feasible and high in application value.

The present invention provides a method and an apparatus for nurturing phalaenopsis orchid seedlings with stalk with a high performance of land use, and comprises a nurture room having an air conditioning equipment for a temperature control; a multi-layer planting bed rack installed in the nurture room for placing the phalaenopsis orchid seedlings; and an illumination rack fixed above each planting bed, and having an artificial light source installed thereon for illuminating the phalaenopsis orchid seedlings in the planting beds. With the aforementioned arrangement, the large quantity of phalaenopsis orchid seedlings placed on each planting bed are grown under the conditions controlled at a temperature at 17° C. ˜25° C. and a light intensity of 100˜11000 Lux, so as to achieve the effects of improving the production efficiency, lowering the cost and providing a stable shipping time.

The invention discloses a plant regeneration method for butterfly orchid, belonging to the field of butterfly orchid planting. The plant regeneration method comprises the following steps of: selecting pedicel axillary bud stems with length of 2-5 centimeters on butterfly orchid plants; carrying out induction culture on stem apexes for 2-3 weeks for gradually forming protocorms after 25-30 days; continuously splitting the protocorms to implement subculture acceleration reproduction; moving seedlings with height of one centimeter to a strong seedling culture medium to be cultured for 3-6 months; and inducing the seedlings to root for 20-25 days, and transplanting the strong seedlings with height of 8-10 centimeters, wherein the survive rate of the seedlings is higher than 95%, nursery-grown plants grow well after surviving. The plant regeneration method enables the excellent butterfly orchids to form sterile strains within a short time for implementing large-scale industrial production, can be produced yearly, has a high reproduction speed, and provides novel, fresh, fragrant and colorful flowers with various colors and consistent model. Moreover, a production technology with high technical content is provided to enterprises, so that the plants are consistent in size for facilitating control on flowering phases and uniform management of industrialization, the production costs of the enterprises are reduced, and giant economical benefits are created.

The invention mainly relates to the field of plant tissue culture and particularly relates to a butterfly orchid anti-browning tissue culture method and an anti-browning culture medium. The tissue culture method comprises the steps of explant disinfection, primary culture, proliferation and differentiation culture, rooting culture, seedling hardening, transplanting and the like, wherein in the primary culture and the proliferation and differentiation culture, extracted solutions of calendulas, lemons, tomatoes, rosemary and the like are added into a corresponding culture medium so as to effectively prevent and treat a common browning problem in butterfly orchid tissue culture; and meanwhile, different types of cutting manners are used for helping to prevent and treat the browning problem at a differentiation phase, so as to form a compound anti-browning method. The method disclosed by the invention has the advantages of high stability, great success rate, greenness and environmental friendliness, and can annularly and repeatedly produce.

The invention relates to a method for using bud to induce the stem to cultivate iris, wherein it comprises that: 1, cultivating the iris until stem; 2, disinfecting the cut stem, cutting into sections with bud, removing package, inserting into cultivate medium; 3, adjusting condition, 4, cultivating in bottle; 5, cutting new stem into section with bud, inserting into cultivate medium, repeating steps 4, 5; repeating steps 5, until there is stem with node bud; 7, cutting stem into section with node bud, inserting into cultivate medium, adjusting condition, inducing bud into nourishment bud; 8, using it as external plant, gaining or separating cultivating; 9, cultivating it on cultivate medium into iris plant.

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting rooting of a butterfly orchid tissue cultured seedling by utilizing an LED light source. The method specifically comprises the following steps: (1) performing induced culture by taking a butterfly orchid flower as an explant to obtain a cluster bud; (2) multiplying and culturing the cluster bud obtained in the step (1), dividing the multiplied and cultured cluster buds into single buds and inoculating the single buds into a rooting culture medium; (3) culturing and rooting the cluster buds which are inoculated into the rooting culture medium in mixed light consisting of three single-color lights including red light, blue light and far-red light. According to the method, the treatment of the mixed light added with the far-red light promotes the growth of the roots of butterfly orchid seedlings, so that accumulation of dry matters is increased, and the growth of the underground parts is not inhibited; meanwhile, the method has the advantages of lowering the production cost and saving energy.

This invention discloses a LED mixed lighting for tissue culture of orchids. Due to limitation of technology, currently it lacks of LED lighting directly used for orchids, especially for tissue culture of dendrobium candidum, ornamental dendrobium and phalaenopsis. This inventions adopts a technical proposal as follows: a LED fixed lighting for tissue culture of orchids is characterized in that said many red and blue LEDs will constitute several units; each unit has 3 blue LEDs and there are 8 red LEDs in elliptical or rounded patterns surrounding said 3 blue LEDs, and said 3 blue LEDs are gathered in triangular pattern, and said 8 red LEDs are separated at interval. This invention makes special arrangement of lamps and realizes adjustment of light quality and light period by turning on or turning off red LEDs and blue LEDs, and by this way, a LED mixed light which is distributed evenly and suitable for tissue culture of orchids.

The invention relates to the technical field of plant cultivation and reproduction, in particular to a method for cultivating and propagating Phalaenopsis, comprising the following steps: material selection, material disinfection, induced cultivation, multiplication and subculture cultivation, strong seedling cultivation and transplanting. Orchids are rapidly cultivated and propagated, and the cultivated Phalaenopsis seedlings have a low incidence rate, a high seedling rate, good seedling quality, and strong adaptability after cultivation. Through biotechnology, they meet the needs of commercial production in a short period of time. , providing technical support for large-scale and industrialized cultivation of Phalaenopsis, and at the same time meeting the needs of the Phalaenopsis cut flower market.

The invention discloses a phalaenopsis brightening mask which is composed of the following components in percentage by mass: 0.64-0.96% of phalaenopsis extract, 5-8% of glycerol, 4-6% of propanediol, 3.6-5.4% of butanediol, 1-3% of sodium ascorbate phosphate, 0.35-0.532% of bis(hydroxymethyl)imidazolidinyl urea, 0.2-0.4% of PEG-60 (polyethylene glycol-60) hydrogenated castor oil, 0.2-0.4% of hydroxyethyl cellulose, 0.05-0.15% of disodium EDTA (ethylene diamine tetraacetate), 0.05-0.15% of essence and the balance of water. By using the phalaenopsis extract as a skin conditioner, the sodium ascorbate phosphate as a freckle remover and PEG-60 hydrogenated castor oil as a surfactant, the phalaenopsis brightening mask has the functions of lightening melanin and brightening the skin, and implements the effects of beauty treatment and skin care.

The invention relates to the technical field of phalaenopsis seedling propagation, in particular to a phalaenopsis virus elimination and rapid propagation method. By exploring of primary culture, stem tip elongation culture, virus elimination culture, seedling strengthening and rooting culture, a rapid propagation approach and a method for secondary phalaenopsis virus elimination on the basis of combination of a physical method and chemical agents. Innovatively, a stem tip elongation culture method and a method for increasing survival rate and virus elimination rate by combination of growing point cutting size and the chemical agents and secondary virus elimination are developed, and a phalaenopsis virus elimination and rapid propagation approach is established. The method has advantages of high survival rate and virus elimination rate, the survival rate is up to 80.13%, the virus elimination rate is up to 92.67%, and remarkable technical effects and promising promotion prospect are achieved.

The invention discloses a phalaenopsis blume culture medium and a culture method. The phalaenopsis blume culture medium comprises a primary induction culture medium and a proliferation subculture medium, wherein the primary induction culture medium takes an MS culture medium as a basic culture medium and is added with 6-benzylamino adenine, naphthylacetic acid, diethylaminoethyl hexanoate, active carbon, cane sugar and agar powder; the proliferation subculture medium takes the MS culture medium as the basic culture medium and is added with 6-benzylamino adenine, naphthylacetic acid, corn oligopeptide powder, coconut milk, active carbon, cane sugar and agar powder. The culture medium disclosed by the invention can be used for enabling phalaenopsis blume to be rapidly propagated, and the gold edge character of the leaves of the phalaenopsis blume can be stably inherited.

The present invention belongs to the field of molecular biology and gene technology, and is especially the nucleotide coding sequence of flavoid-3', 5'-hydroxylase gene expressed in butterfly orchid, and its application in changing flower color. The present invention relates to the recombinant expression vector including the said gene, the transgenic plant, nucleotide primer sequence for obtaining the gene, the oligonucleotide sequence probe for detecting endogenous expression mode and method of analyzing and identifying the expression mode of the endogenous gene in butterfly orchid. Transforming the gene into petunia obtains transgenic plant with differentially expressed flavoid-3', 5'-hydroxylase gene and altered flower color.

The invention relates to the technical field of non-toxic cultivation of plants, in particular to a phalaenopsis non-toxic seedling cultivation and propagation method. The phalaenopsis non-toxic seedling cultivation and propagation method comprises the following steps: performing heat treatment on the plants, selecting materials, disinfecting the materials, performing pre-treatment on the materials, and performing tissue cultivation. The tissue cultivation comprises protocorm induced cultivation, multiplication cultivation, strong seedling cultivation and transplanting. After the phalaenopsis seedlings are cultivated according to the method, the disease incidence rate is low, the survival seedling rate is high, the seedling quality is high, the adaptability of the seedlings is high after being planted, the seedlings grow well, the requirements on commercialized production are met in a short time through a biotechnological means, and technical support is provided for large-scale and industrialized cultivation of the phalaenopsis non-toxic seedlings of enterprises.

The invention discloses a gene sequence for regulating and controlling the petal color of phalaenopsis equestris. The gene sequence comprises one or a plurality of the following nucleotide sequences:1, a nucleotide sequence shown in SEQ ID NO.1; 2, a nucleotide sequence generated by substitution, deletion or addition derivation of one or a plurality of nucleotides of the nucleotide sequence shownin SEQ ID NO.1; and 3, a nucleotide sequence with homology of at least 80% with that in SEQ ID NO.1. The gene sequence PeMYB4 disclosed by the invention has an important effect of regulating and controlling the flower color development of the phalaenopsis equestris, and in a VIGS strain of the phalaenopsis equestris, the color of the phalaenopsis equestris is obviously lightened, so that an important foundation is provided for the breeding of the phalaenopsis equestris.

The invention discloses a method for regulating the flowering period of phalaenopsis tissue culture seedlings. The method comprises the following steps that 1, roots of the phalaenopsis seedlings arewrapped in sphagna, placed in a 1.5 L nutrient bowl and cultivated in a greenhouse until new roots grow out, and then fertilization is conducted; 2, when the roots are completely wound in sphagna, the1.5 L nutrient bowl is replaced with a 3.5-inch nutrient bowl, and 10 days after bowl replacement, fertilization is conducted once every 10 days; 3, an abscisic acid solution is adopted for wateringthe roots of phalaenopsis plants to promote dormancy of the phalaenopsis plants; 4, 120 days before expected marketing and selling, flower buds are induced, a GA3 solution and a KH2PO4 solution are adopted for watering the roots of the phalaenopsis plants in the early stage of flower bud induction, a compound fertilizer solution is adopted for watering the roots of the phalaenopsis plants in the later stage of the flower bud induction, and after natural illumination in this stage is completed, a combined LED red-blue light source is adopted for illuminating phalaenopsis; 5, in a flower bud differentiation period, a 6-BA solution is adopted for watering the roots of the phalaenopsis plants. The method is adopted for regulating the flowering period of the phalaenopsis tissue culture seedlings to obtain phalaenopsis which has a controllable flowering time and neater and brighter flowers.

A preparation method of phalaenopsis nutrient fertilizer, which is composed of: pigeon dung powder, rabbit dung powder, sorghum cake powder, rapeseed cake powder, tomato stem and leaf powder, carrot stem and leaf powder, wheat bran powder, peanut shell powder, Bone meal, dried chicken manure, sesame cake powder, pumpkin powder, safflower powder, pine leaf powder, vanilla powder, onion powder, Chuanwu powder, and the percentages described are percentages by weight; The extraction, preparation and preparation methods are simple, and the various nutrients in the ingredients are rich in content, so that the phalaenopsis grows fast, has strong growth power, and has good disease prevention performance.

The invention discloses a method for rapidly propagating Phalaenopsis by inducing clustered buds through leaves, in particular a method for sterilizing and cultivating Phalaenopsis pedicel buds to obtain aseptic materials, removing leaves from the aseptic materials, and germinating aseptic pedicel buds to obtain Cluster buds, while the cluster buds propagate, utilize aseptic materials or / and leaves in the cluster buds to induce cluster buds, and finally use the cluster buds as breeding objects to cultivate tissue culture seedlings to rapidly propagate Phalaenopsis. The method has the advantages of fast propagation speed, short growth cycle and reduced production cost.

After butterfly orchid is treated by an outdoor hormone combination (TDZ+GA3+NAA) and a browning prevention solution (AC+PVP+Vc0.5g / L+glutathione), adventitious buds can be directly induced, the content of explant total phenols and PAL activity can be inhibited, and rapid plant growth is promoted. Due to the outdoor hormone treatment, hormones can be absorbed by the explant in advance, hormonal equilibrium needed by adventitious bud germination is achieved, and the time needed for absorbing nutrition in a bottle is shortened. Browning generated in the explant is greatly inhibited due to the outdoor treatment, so that rapid growth of the adventitious buds and seedlings is promoted. According to the explant, the adventitious buds of the butterfly orchid are directly induced without passing a callus stage, excellent traits of stock plants can be kept, variation generated in the culture process is reduced, and the method has high significance in large-scale production.

The invention relates to a novel cultivation method for butterfly orchid tissue culture seedlings, and belongs to the technical field of flower culture, particularly orchid cultivation. The novel cultivation method comprises the following steps: (1), preparing a cultivation medium: mixing coconut fiber and volcanic rock after being processed to prepare a cultivation medium, or mixing the coconut fiber and float grass imported from Chile, after being processed, to prepare the cultivation medium, or mixing the volcanic rock and the float grass imported from Chile, after being processed, to prepare the cultivation medium, or mixing the coconut fiber, the volcanic rock and the float grass imported from Chile, after being processed, to prepared the cultivation medium, or preparing the coconut fiber after being processed into the cultivation medium; (2), getting a culture flask for loading butterfly orchid tissue culture seedlings, taking the butterfly orchid tissue culture seedlings out of the culture flask, cutting off dead roots and yellow leaves, washing root systems to be clean, and obtaining butterfly orchid tissue culture seedlings to be transplanted; the cultivation medium adopted in the invention has the advantages that degradation products are free from contamination, and good water holding, ventilating, moisture-holding, fertilizer retaining functions are achieved, and the requirements of the growth of the butterfly orchid are met; moreover, the denudation of microbes can be resisted, the probability of rottenness is low, and the cost is low.

The invention belongs to the field of plant tissue culturing, and in particular relates to a method for promoting rapid propagation of cluster buds of a butterfly orchid by utilizing an LED (Light Emitting Diode) light source. The method comprises the following steps: (1) inducing the generation of cluster buds by taking a pedicel of the butterfly orchid as an explant; (2) cutting the cluster buds obtained in the step (1) into simple buds or bunch buds and inoculating the simple buds or the bunch buds into an enrichment medium; and (3) by taking an LED capable of emitting red light or emitting warm white light as a light source, culturing the cluster buds which are obtained in the step (2) and are inoculated into the enrichment medium under the LED capable of emitting red light or emitting warm white light. According to the method, the number of the single buds of the cluster buds is obviously increased; the dry weight, the fresh weight and the plant height of the cluster buds are all increased; and the production cost is lowered and energy conservation is realized.

The invention relates to a seedling hardening and domestication method in greenhouse for forest tree tissue culture seedlings. The method is characterized by comprising following steps: (1) seedling hardening greenhouse preparation; (2) seedling hardening management of tissue culture seedlings before bottle out; (3) cultivation substrate and container preparation; (4) tissue culture seedling transplanting; (5) domestication management after transplanting; (6) domestication time control after transplanting. According to the seedling hardening and domestication method in greenhouse for forest tree tissue culture seedlings, a uniform and stable domestication environment is formed with white transparent nursery pot used for butterfly orchid production and plates of the corresponding size. Compared with the prior art, the method is simple and efficient; the plates and the nursery pots can be reused, which greatly reduce production cost and can be promoted in large scale; according to the measure integrated effect before, during and after the seedling hardening; the time for seedling hardening after tissue culture seedling transplanting in greenhouse can be shortened by at least one month; the survival rate of seedling hardening is increased by 5 to 15 percentage; the manpower and material cost is reduced by about 30%; the over ground part of the tissue culture seedlings is increased by 30 to 50% compared with seedlings planted by regular methods. The plant grows well.

The invention belongs to the technical field of polyploid breeding, and discloses a method for inducing phalaenopsis protocorm to generate polyploid by colchicine. The method comprises the following steps of transferring the protocorm into a 1 / 2MS+1mg / l 6-BA + 0.2 mg / l NAA+5g / l agar+20g / l sucrose solid culture medium containing 0.05% of colchicine, and culturing for 15 days for induction treatment; washing the protocorm for 3-4 times by using sterile water after treatment, absorbing moisture by using filter paper, transferring the protocorm into a 1 / 2MS+5g / l agar+20g / l sucrose culture medium,and carrying out subculture for 2-3 times; performing polyploid identification, namely respectively carrying out morphological observation, stomatal identification and ploidy identification; and performing data statistics. According to the method, colchicines with different concentrations and different treatment time are used for doubling the phalaenopsis protocorm by a co-culture method, so thata foundation is laid for polyploid induction culture of a new phalaenopsis variety with large flower size, bright color and high ornamental value.

The invention relates to the technical field of plant nursery stock cultivation, in particular to a management method for promoting prematurity of potted butterfly orchid. The management method comprises the following steps: removing a butterfly orchid seedling which is 1.5 inches from a pot once every 50 to 52 days, immersing the seedling in a purple non-sulfur germ secreta extracting solution, wrapping the surface of each butterfly orchid leaf with a layer of blue polyethylene film, carrying out treatment with dark light, weak light, moderate light and strong light cyclically in sequence for 4 to 5 cycles, and after the butterfly orchid is bloomed, and watering the root of the butterfly orchid once with a solution with sodium alizarin sulfonate diluted by 800 times every other 5 days. After being treated by the management method, the butterfly orchid seedling which is 1.5 inches can be bloomed in advance within 5 to 6 months, and the flowering phase can be lasted for 60 to 75 days.

The invention discloses a tissue cultural method of butterfly orchid. The method comprises the steps of seedling cultivation of proliferation nutrient solution which includes compound major elements, compound micro substances, inositol, 6-benzyl purine, 2,4-dichlorobenzene acetic acid, indolebutyric acid, mashed potatoes and saccharose; strong seedling cultivation of strong seedling culture solution which contains compound major elements, compound micro substances, inositol, peptone, activated carbon, citric acid, ammonium nitrate, nitrate of potash, mashed potatoes, mashed bananas and saccharose; adaptation cultivation of growing culture solution which comprises compound major elements, compound micro substances, inositol, indolebutyric acid, peptone, activated carbon, citric acid, ammonium nitrate, nitrate of potash, major element auxiliaries, mashed potatoes, mashed bananas and saccharose. A culture medium of butterfly orchid is also provided in the invention, and by using the culture medium, the germination rate is increased by 6%; the growing period of seedlings is shorten by 10 days; the survival rate of the seedlings is 100%; the growing period of the strong seedlings is shorten by 14 days.

The invention discloses a butterfly orchid induction culture medium and an asexual propagation method of butterfly orchid. The butterfly orchid induction culture medium is obtained by mixing a standard N6 culture medium serving as basic mother liquor with 6-benzylaminopurine BA, gibberellin GA3 and an organic additive. The butterfly orchid induction culture medium can be used for inducing pedicel internode parts of butterfly orchids to quickly propagate the butterfly orchids and has the advantages of low cost, high protocorm-like body induction rate, short induction time, high growth speed and the like. According to the asexual propagation method of the butterfly orchid, tender pedicel internodes are used as an explant, and the butterfly orchid induction culture medium is used as the culture medium; by the adoption of the method, pedicel materials are fully used; waste is turned into treasure, and the cost is reduced; furthermore, the parent is not damaged; the flowers on the lower parts of the pedicels can be still used for hybridization.

The invention discloses a bactericide composition. The bactericide composition contains pyrimethanil and berberine, for 1 part of pyrimethanil, the content of the berberine is 0.01-90 parts by weight. The invention further provides application of the bactericide composition in prevention and control of botrytis of cucumbers, tomatoes, strawberries, grapes, rieger begonia, cyclamen, cineraria, phalaenopsis, poinsettia pulcherrima, peony, China aster, camellia, chrysanthemums, begonia, dahlia, geranium and gladiolus. Through the adoption of the technical scheme, the bactericide composition has obviously enhanced prevention and control effect with respect to the pyrimethanil and berberine.

The invention discloses a TUA segment sequence for a Phalaenopsis internal reference gene. Multisequence comparison degenerate primer design, PCR (polymerase chain reaction) amplification and sequencing are performed to obtain the alpha-tubulin gene (TUA) segment sequence; and according to the designed fluorescence quantitative specific primers, real-time fluorescence quantitative PCR detection is performed to determine that the TUA segment sequence is an internal reference gene which can be stably expressed in different tissues in different Phalaenopsis development stages.

The invention discloses a dendrobium candidum suspension-type cultivation method. A butterfly orchid pallet is employed to make a cultivation device, the cultivation device is filled with pine bark which serves as a cultivation medium, dendrobium candidum seedlings of the right age are planted, plastic bands are used as binding and suspension materials, the cultivation device is suspended on appropriate cultivation places such as the underneath of trees, the interior of cultivation facilities or balconies and roofs with shading facilities, and the suspension-type cultivation of dendrobium candidum is achieved. According to the invention, a dendrobium candidum cultivation new selection is provided, compared with other cultivation methods, the dendrobium candidum suspension-type cultivation method is characterized by being simple to operate, convenient to manage and protect, and low in plantation cost, and being flexible. According to the invention, scattered forest trees and limited family space can be fully utilized to carry out the suspension-type cultivation of dendrobium candidum, the cultivation device can be adjusted flexibly according to the change of environmental factors, and the growth of dendrobium candidum can be facilitated; generation and development of diseases and pests can be effectively prevented, and usage of pesticides can be avoided or reduced. The dendrobium candidum suspension-type cultivation method has a promotion effect on promoting scattered plantation under trees and household plantation of dendrobium candidum.