The invention relates to a method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo, comprising the following steps of: (1) culturing mycorrhizal fungi by utilizing a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP2 deuteromycotina Fusariumsp, mycorrhizal fungi LP3, or mixed strain deuteromycotina Verticilliumsp of the mycorrhizal fungi LP2 deuteromycotina Fusariumsp and the mycorrhizal fungi LP3; (2) culturing tissue culture seedlings of the dendrobium officinale kimura et migo; (3) preparing a nutrient solution; (4) carrying out water culture and training the tissue culture seedlings; and (5) carrying out planting and mycorrhizalculturing of the dendrobium officinale kimura et migo. The method for culturing, planting and mycorrhizal production of the dendrobium officinale kimura et migo, provided by the invention, can promote growth of dendrobium officinale kimura et migo seedlings obviously; and compared with a contrast plant, the dendrobium officinale kimura et migo cultured by inoculating pathogen has increased rooting numbers, high survival rate, longer plant length, better growth vigor, obviously expanded internodes, obviously big stems, improved fresh weight and dry weight and obviously enhanced stress resistance of a pathogen-inoculated plant.