564 results about "Agricultural biotechnology" patented technology

The invention relates to the technical field of agricultural biologics and in particular relates to Bacillus velezensis ANSB01E CGMCC No. 17328 and application thereof. The Bacillus velezensis provided by the invention is capable of simultaneously and efficiently degrading zearalenone and aflatoxin, and degradation rates upon g zearalenone and aflatoxin are both up to 90% or greater 48 hours laterafter reactions. The Bacillus velezensis provided by the invention is high in toxin degradation efficiency, good in specificity, mild in action effect, free of damage to nutrient components in feedsand applicable to biological detoxification of mycotoxins in cereals, feeds and foods.

The invention belongs to the field of agricultural biotechnology, and particularly relates to a zearalenone degrading enzyme fusion protein and a coding gene thereof and application thereof. The zearalenone degrading enzyme fusion protein is composed of a zearalenone degrading enzyme ZHD101 and xylanase XYNB, and an amino acid sequence of the zearalenone degrading enzyme ZHD101 is as shown in SEQID No. 1, and an amino acid sequence of the xylanase XYNB is as shown in SEQ ID No. 2. According to the zearalenone degrading enzyme fusion protein and the coding gene thereof and the application thereof, the expression level of the zearalenone degrading enzyme is greatly increased, and the enzyme activity is 2.2 times that of ZHD101 expressed alone.

Belonging to the field of agricultural biotechnology, the invention relates to a laodelphax striatellus lethal gene fragment. By a laboratory improved dsRNA feeding method, the invention, from laodelphax striatellus, screens out the laodelphax striatellus lethal gene fragment Ribosomal protein L9-B which can lead to laodelphax striatellus death and has sequences as shown in SEQ ID No.1. Feeding laodelphax striatellus with the dsRNA of the gene fragment provided in the invention can have good lethal effect, thus providing sequence and data bases for establishing new strategies of pest control with RNA interference technology.

The invention belongs to the field of agricultural and biological technology and relates to a gene silencing technique based Laodelphax striatellus lethal gene fragment Alpha1-tubulin and a dsRNA thereof. The invention is to screen the Laodelphax striatellus lethal gene fragment Alpha1-tubulin, which can lead to the death of Laodelphax striatellus after interference and has a sequence representedby SEQ ID No.1, from Laodelphax striatellus by using a laboratory improved dsRNA feeding method. When the dsRNA of the gene fragment is used for feeding Laodelphax striatellus, the adjusted mortalityof the Laodelphax striatellus may reach 70 percent, and a sequence basis and a data basis are provided for the construction of a new policy of controlling pests by using RNA interference technology.

The invention discloses a method for quickly extracting DNA from plant leaves, and relates to a method for quickly extracting beet total DNA by using leaf tissues. The method comprises the following steps: directly grinding leaves in a grinding pestle centrifuge tube, performing high-temperature water bath treatment by using an extracting buffer solution, and adding a separation medium to perform high-speed centrifugation. The method disclosed by the invention is simple in step, less in required tissue sample, less in toxic chemical reagent, low in cost, short in operation time and good in experimental effect. The obtained DNA can be directly used for PCR (polymerase chain reaction) amplification of nucleoplasm gene fragments, and applied to molecular biology tests of molecular markers, fingerprint spectrums and the like, so that a DNA nucleic acid base material is provided for agricultural biotechnology molecular breeding and researches in the field of molecular biology.

The invention relates to a multi-seedling autogenic relay growth method for seeds of amorphophallus muelleri, and belongs to the field of agricultural biotechnology. The multi-seedling autogenic relay growth method includes 1, processing the seeds; 2, soaking the seeds in solution containing 3% of potassium permanganate by 30 minutes to bactericide the seeds; 3, soaking the seeds in cold water for three days; 4, soaking the seeds in thiourea with the concentration of 0.1-3% or mixed liquid containing 400ppm GA3, 33ppm IBA and 33ppm 6-BA for 12 hours; and 5, breaking dormancy of the seeds and sowing the seeds. In the procedure for breaking the dormancy of the seeds and sowing the seeds for breeding seed bulbs, the moisture content of soil keeps 25-45% after the seeds are sown in the soil. As shown by test results, a germination rate of the seeds can reach 95% at least after seed dormancy breaking, an emergence rate of the seeds reaches 90% at least, the maximum weight of harvested seed bulbs of the amorphophallus muelleri is 710g, and the average weight of the harvested seed bulbs of the amorphophallus muelleri is 500g. The multi-seedling relay growth method has the advantages that a procedure is simple, and operation is convenient; by combining dormancy breaking with a germination accelerating and seedling raising mode, the germination speed and the germination rate of the seeds of the amorphophallus muelleri can be effectively increased, and the germination uniformity of the seeds of the amorphophallus muelleri can be effectively improved; and the quality of spouts is improved, the amorphophallus muelleri production period is shortened, and the method can be efficiently applied to large-scale production for commercial amorphophallus muelleri or seed bulbs in amorphophallus muelleri plantation.

The invention relates to a preparation method of biological bacteria fertilizer for improving stress resistance of plants, which belongs to the technical field of agricultural biology; and the preparation method is characterized in that the biological bacteria fertilizer consists of following components by weight percent that: 5 to 10 percent of biological bacteria strain, 20 to 30 percent of animal excrement, 40 to 50 percent of pool mud, 10 to 15 percent of decomposed coal and water in balance. The preparation method comprises following steps that the raw materials are adequately stirred and mixed according to the proportion and placed inside a fermentation pond to be thermo-modulated and fermented for 30 days, after the fermentation is completed, the material is crushed and filtered by a 100-mesh coarse sieve to obtain the powder preparation, and the prepared preparation is placed inside a packaging bag with a moistening function, so that the bagged solid bacteria fertilizer product is formed. The preparation method has characteristics of simplicity, convenience in obtaining of raw materials, low cost and good economic benefit.

The invention relates to an screening method in vitro of elephant grass salt-tolerant somatic mutant belongs to a field of agricultural biotechnology. The process is: granular callus is transferred into proliferation medium with 16g / L NaCl, into differentiation medium with 16g / L NaCl after 45 days, and into rooting medium with no NaCl after 35 days to get regeneration plant; pot salt-tolerance identification of the regeneration plant is applied after tidal flat field comprehensive agronomic characters evaluation and grass-production measurement to get plants of which the fresh grass yield is higher than a control by 23.8% in tidal flat field with pH8.67-9.01; the fresh grass increment of the first time and the second tiome of cutting for the clonal progeny of the plants is higher than the control by 9.2% and 7.4% respectively in 1 / 2Hoagland nutrient solution with 8.25g / L sea-salt; therefore, the salt-tolerant somatic mutant of good comprehensive agronomic characters and significantly high salt-tolerance is selected. The invention has the advantages of simple-operation, short-cycle, and high selection efficiency.

The invention relates to a method for producing polypeptide antibiotics enramycin by the fermentation method, which pertains to the fields of agricultural biotechnology, industrial biotechnology and the fermentation engineering. The method is as follows: (1) slant culture: a Streptomyces sp.NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days; (2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days; (3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.