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Pseudomonas putida SRPG-396 and salt-dissolving and growth-promoting application thereof

A technology of Pseudomonas putida and strains is applied in the field of agricultural biology to achieve the effects of strong adaptability, increasing economic income and alleviating the harm of salt stress

Inactive Publication Date: 2015-04-22
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in view of the special climate and geographical environment in Northwest China, there is no report on the development of highly efficient salt-dissolving and growth-promoting strains and their potential microbial agents suitable for application in arid and semi-arid areas.

Method used

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  • Pseudomonas putida SRPG-396 and salt-dissolving and growth-promoting application thereof
  • Pseudomonas putida SRPG-396 and salt-dissolving and growth-promoting application thereof
  • Pseudomonas putida SRPG-396 and salt-dissolving and growth-promoting application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The separation and purification of embodiment 1 bacterial strain

[0027] Soil samples were collected from the cotton rhizosphere soil of Xinjiang saline-alkali land and salinized cultivated land caused by long-term drip irrigation. Weighed 10g of soil samples, put them into 100ml of sterile water, added sterilized glass beads, 200rpm, 30°C, and shook for 20min. The sample is well dispersed. Aseptically draw supernatant 100μl into 900μl sterile water, dilute with sterile water to make 10 -2 --10 -8 concentrated diluent. Take 100 μl of dilutions of each concentration, apply the conventional coating method on the NA plate, and place it in a constant temperature incubator at 30°C for cultivation. After 48 hours, after colonies grew on the plate, a single colony on the plate was picked for purification and culture to obtain strain SRPG-396.

Embodiment 2

[0028] Example 2 Screening of salt-dissolving and growth-promoting bacterial strain SRPG-396

[0029] (1) Primary screening

[0030]Preparation of bacterial suspension: the bacterial strains were inoculated in liquid NA medium respectively, and after being shaken at 200 rpm for 2 days at 30°C, 10 8 Bacteria suspension with cfu / ml concentration is ready for use.

[0031] Experimental treatment: select cotton seeds that are plump and of the same size, sterilize them with 75% alcohol for 15 seconds, then soak them in 0.1% mercuric chloride for 20 minutes, wash them with sterile water for 3 times, soak them in the bacterial suspension for 4 hours, and then sow the cotton seeds in In the hole tray equipped with sand, the water content of the sand was adjusted to 14% with 0.5% sodium chloride solution in advance, and 5 cotton seeds were one repetition, and each treatment had four repetitions. Placed in an artificial intelligence climate box for cultivation. (Day and night tempera...

Embodiment 3

[0040] Identification characteristics of embodiment 3 salt-dissolving growth-promoting bacterial strain SRPG-396

[0041] According to the morphological characteristics of the bacteria, the morphological characteristics of the colonies and the results of Gram staining, combined with the eighth edition of Bergey's Manual of Etermitive Bacteriology and the Manual of Identification of Common Bacteria, the salt-dissolving and growth-promoting bacteria SRPG-396 The identification of physiological and biochemical characteristics was carried out. The results are shown in Table 3. After preliminary identification, the strain SRPG-396 belongs to the genus Pseudomonas.sp.

[0042] Table 3 Physiological and biochemical characteristics of strain SRPG-396

[0043] Pilot projects

result

Pilot projects

result

Colony shape

round bump

gas production

+

Cell shape

short to round

glucose

+

Gram

－

glucose acid productio...

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Abstract

The invention relates to pseudomonas putida SRPG-396 and salt-dissolving and growth-promoting application thereof, and belongs to the field of agricultural biotechnology. The accession number of the bacterial strain is CGMCC No. 9397. The bacterial strain has the functions of dissolving inorganic phosphorus, and secreting auximone and siderophore, can improve the absorption of elements such as nitrogen, phosphorus, potassium, calcium, iron, and the like by crops, reduce the absorption of sodium, has good salt resistance, can effectively relieve salt stress of crops caused by salinized soil and promote growth of crops under salt stress. When compared with a control, the bacterial strain can significantly improve the germination rate, dry weight, and plant height of crops such as cotton, tomatoes, capsicum and the like under salt stress. The bacterial strain of the invention has the advantages of stable functions, strong environment adaptability, and ecological environment friendliness, can be prepared into products such as microbial agents, biological fertilizers, and the like, has great development potential for industrial products, and has good application prospects.

Description

Technical field: [0001] The invention relates to a strain of Pseudomonas putida with salt-dissolving and growth-promoting effects and its application in alleviating the salt stress damage caused by salinized soil to crops and promoting the growth of crops at the seedling stage, belonging to the field of agricultural biotechnology. Background technique: [0002] Northwest China is located in an arid and semi-arid region, and water shortage has become a bottleneck restricting its agricultural development. At present, the local area combines advanced drip irrigation technology with film-covered planting mode to form an efficient water-saving technology-under-film Drip irrigation has fundamentally changed the traditional agricultural structure and greatly promoted the development of agricultural production. However, at the same time, the soil salt in the cultivated layer cannot be fully washed. As the evaporation continues to move upward, the salt accumulates in the cultivated l...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/40
CPCA01N63/00C12N1/205C12R2001/40
Inventor 李春樊艳爽武占省何艳慧凃亮
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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