50 results about "Bacteriology" patented technology

The invention relates to a rapid parallel nucleic acid detection method and system based on a micro-fluidic chip. The nucleic acid detection system comprises a micro-fluidic chip, a motor, exciting light, a double-focal-plane imaging lens set, a detector, a signal acquisition processor and a display; the micro-fluidic chip comprises at least one reaction channel; a heating film is arranged at the periphery of the micro-fluidic chip; a submillimeter air layer is maintained between the micro-fluidic chip and the heating film; and the micro-fluidic chip is irradiated by adopting the exciting light, so that a nucleic acid sample generates fluorescence under excitation of the exciting light, the fluorescence is gathered on the detector by virtue of the double-focal-plane imaging lens set so as to generate an analog signal, the detector transmits the generated analog signal to the signal acquisition processor so as to generate a real-time fluorescence detection signal, and the real-time fluorescence detection signal is displayed by the display. The method and the system disclosed by the invention can be applied to the fields of clinical pathogenic bacterium molecular diagnosis, food inspection and quarantine, food poisoning pathogenic bacterium detection, bacteriology classification and epidemiological investigation and have huge economical and social benefits.

The invention discloses an oligonucleotide primer for detecting common pathogenic bacteria by adopting a fluorescent quantitation PCR (Rich Client Platform) technology, a method thereof for detecting common pathogenic bacteria and the application thereof. The method comprises the following steps of: providing 10 pairs of specific oligonucleotide primer sequences at annealing temperature of 50-60 DEG C without differing 5 DEG C; and simultaneously, quickly, accurately and effectively identifying and quantificationally detecting various pathogenic bacteria at the same time. A detection range comprises bacillus cereus, enterobacter sakazakii, vibrio parahaemolyticus, enterohemorrhagic escherichia coli O157, salmonella, Listeria monocytogenes, Shigella, campylobacter jejuni, pseudomonas aeruginosa, klebsiella pneumoniae, and the like. The invention also can be used for the fields of disease diagnosis, environmental monitoring, water-quality and food supervision and detection, food poisoning pathogenicbacteria detection, bacteriological classification, epidemiological investigation, biological agent detection, and the like, is convenient, quick, accurate and effective and has wide application range.

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

The present invention relates to the field of bacteriology. In particular, the invention relates to novel compositions and methods for altering (e.g., inhibiting) the growth and virulence of populations of pathogenic microorganisms.

The invention relates to a microbial detection device and a detection method of meat of livestock and poultry. The salmonella and Escherichia coli O157 multiple PCR rapid detection kit of the meat of livestock and poultry includes: 10 multiplied by PCR buffer, 1.0 to 3.0mmol / L of MgCl2, 240 Mumol / L of dNTP each, 200nmol / L of salmonella primer, 200nmol / L of Escherichia coli O157 primer and 1.3 to 3.0U of Taq enzyme. The microbial detection device has better specificity, which can complete the simultaneous detection of the salmonella and the Escherichia coli O157 within 8 to 9h; compared with the conventional bacteriology, the diagnosis is rapid, convenient and economical, thus providing a powerful technical means for the diagnosis of pathogen in a food sample and having good application prospect.

The present invention relates to the field of bacteriology. In particular, the invention relates to novel compositions and methods for altering (e.g., inhibiting) the growth and virulence of populations of pathogenic microorganisms.

The invention discloses a PCR (Polymerase Chain Reaction) amplification primer for quickly detecting klebsiella pneumonia and application thereof, and belongs to the field of animal bacteriology and molecular biology. The PCR amplification primer is prepared from an upstream primer having a nucleotide sequence as shown in SEQ ID NO:1 and a downstream primer having a nucleotide sequence as shown inSEQ ID NO:2; the PCR amplification primer is used for preparing a PCR amplification kit for detecting the klebsiella pneumonia. A PCR detecting method provided by the PCR amplification kit has high specificity and sensitivity, good repeatability and high credibility and is capable of specifically detecting the klebsiella pneumonia and quickly and accurately obtaining a detecting result; meanwhile, the PCR amplification kit is low in cost and simple in operation, is suitable for being used at the primary level and can be used as a quick, accurate and simple detecting tool for quick laboratoryidentification of the klebsiella pneumonia and large-scale epidemiological investigation.

The invention relates to the field of animal bacteriology and provides a preparation method of an inactivated vaccine of A-type clostridium perfringens for clostridium welchii disease of a domestic rabbit. The preparation method comprises the following steps: adopting the A-type clostridium perfringens as an original strain, finally obtaining a bacterium solution by primary and secondary culture, mixing the bacterium solution and white oil adjuvant and preparing the inactivated vaccine of the A-type clostridium perfringens. The vaccine prepared by the method is immunized for the domestic rabbit, the protective rate reaches 100% after counteracting toxic substances, so that good immunoprotection action is achieved.

The invention relates to nucleotide special to an Internal transcribed spacer (ITS for short) of a 16S rRNA-23S rRNA gene in Proteus mirabilis, in particular to oligonucleotide special to the ITS in the Proteus mirabilis and application thereof. The invention also provides a PCR detection reagent taking an oligonucleotide pair as a primer and a detection method thereof. The detection method utilizes the PCR reagent to detect the Proteus mirabilis in human body and the environment, is simple, convenient and quick, has good specificity and high sensitivity, can be used in the fields of supervision and detection of food and clinical samples, detection of pathogen in the food, microbial classification and epidemiological investigation and so on, and has deep social benefit and large economic benefit.

The present invention is a modular electronic-optical-mechanical apparatus, to be used by observers in combination with a wide variety of optical image sources, for observing, acquiring and sharing the optical imagery of objects, and the audio and sounds derived from observing sessions bi-directionally in real time with audiences both remote and local. The apparatus enables observers who are local or remote from optical image sources to control and acquire optical imagery from optical image sources, where those optical image sources include microscopes, telescopes, IR scopes, spotting scopes, polarimeters, interferometer microscopes, interferometers, rifle scopes, surveillance scopes, drone optics, binoculars, theodolites, autocollimators, alignment telescopes, camera lenses, periscopes, slit lamp bio microscopes and dioptometers. The observer's audiences span the fields of training, teaching, surveying, bird watching, hunting, target shooting, photography, law enforcement, remote surveillance, local surveillance, drone flight, national defense, medicine, metrology, interferometery, astronomy, geology, biology, bacteriology, ophthalmology, entertainment, et al.

The present invention relates to the field of bacteriology. In particular, the present invention provides compositions (e.g., a lantibiotic-based spore decontaminant (e.g., comprising nisin)) and methods of neutralizing (e.g., killing or inhibiting growth or inhibiting germination of) bacteria (e.g., cells and spores). For example, the present invention provides nisin-based compounds (e.g., for bacterial spore decontamination) and methods of using the same in research, therapeutic and drug screening applications.

The invention relates to a primer for respectively amplifying specific regions of gyrB genes (DNAgyrase B subunit gene, hereinafter referred to as gyrB) in Legionella micdadei, Legionella bozemanii and Legionella longbeachae, but also provides a PCR (Polymerase Chain Reaction) reagent kit comprising the primer for amplifying the specific regions of gyrB genes in Legionella micdadei, Legionella bozemanii and Legionella longbeachae. The PCR reagent kit for detecting Legionella micdadei, Legionella bozemanii and Legionella longbeachae is simple, convenient and quick and has good specificity and high sensitivity, can be applied to the fields of supervision and detection of water bodies and clinic samples, detection of pathogenic bacteria in drinking water, bacteriology classification, epidemiology survey and the like and has profound and lasting social benefit and great economic benefit.

The invention relates to nucleotides SEQ ID NO: 1 to SEQ ID NO: 3 with specificity to the 16S rRNA-23S rRNA internal transcribed spacer (ITS) of neisseria meningitidis and application thereof, and provides a fluorescent quantitative PCR(polymerase chain reaction) detection kit and a detection method by using oligonucleotide as a primer and a probe. The fluorescent quantitative PCR detection kit has the advantages of simple operation, quick result, good specificity and high sensitivity when used for detecting the neisseria meningitidis in a human body and environment, can be used in fields such as supervision and detection of foods and clinical samples, detection of pathogenic bacteria of foods, bacteriological classification and epidemiological study and has significant social benefit andgreat economic benefit.

The invention relates to a primer for amplifying the gene specific area of a DNA gyrase B subunit gene (gyrB for short) in legionella pneumophilia and provides a polymerase chain reaction (PCR) kit which comprises the primer for amplifying the gene specific area of the gyrB in the legionella pneumophilia. The PCR kit is simple, convenient and rapid and has high specificity and sensitivity when used for detecting the legionella pneumophilia, can be applied to the fields such as the supervision and detection of water bodies and clinical samples, the detection of pathogenic bacteria in drinking water, bacteriology classification, the investigation of epidemiology and the like and has good social benefit and economic benefit.

The present invention relates to the field of animal bacteriology, and provides an indirect ELISA detection method of a clostridium perfringens type A toxin antibody. The method uses prepared clostridium perfringens type A toxin as an antigen; the antigen is used for immunizing rabbit to prepare a hyperimmune serum, and is also concentrated and purified as a coating antigen for indirect ELISA. The detection method includes the following steps: (1) coating; (2) enclosing; (3) adding a sample to be tested; (4) adding a secondary antibody; (5) developing; (6) terminating; and (7) determining the results. The method has the advantages of high specificity, strong sensitivity, good repeatability and low cost. In addition, the method can detect samples in a large amount, and provides an effective and simple serological diagnosis method for the detection and study of the disease.

The invention belongs to the technical field of animal bacteriology and vaccine genetic engineering preparation, and relates to a resistance mark-free recombinant bordetella bronchiseptica strain for expression of a pig toxaigenic pasteurella multocida toxA gene fragment, and its vaccine containing the resistance mark-free recombinant bordetella bronchiseptica strain, a preparation method and a use. The resistance mark-free recombinant bordetella bronchiseptica strain QH0814 delta aroA / toxA-N for expression of the pig toxaigenic pasteurella multocida toxA gene fragment does not contain a 5-enolpyrul-shikimate-3-phosphate synthase aroA gene of bordetella bronchiseptica, and contains the pig toxaigenic pasteurella multocida toxA gene fragment. The invention also discloses the preparation method of the resistance mark-free recombinant bordetella bronchiseptica strain and the vaccine use. The genetic engineering vaccine can stimulate a pig to produce a protective immune response for resisting pig toxaigenic pasteurella multocida and a wild strain of pig bordetella bronchiseptica, and can effectively prevent the double infection caused by pig toxaigenic pasteurella multocida and pig bordetella bronchiseptica.

The invention discloses a PCR amplification primer for the rapid detection of a bovine infectious rhinotracheitis virus and applications thereof, and belongs to the fields of animal bacteriology and molecular biology. The PCR amplification primer is composed of a forward primer having a nucleotide sequence represented by SEQ ID No.1 and a reverse primer having a nucleotide sequence represented bySEQ ID No.2. The PCR amplification primer is used to prepare a PCR amplification kit for detecting the bovine infectious rhinotracheitis virus. The provided PCR detection method using the kit has theadvantages of high specificity, high sensitivity, good repeatability, and high credibility. The bovine infectious rhinotracheitis virus can be detected specifically. The detection results can be obtained rapidly and accurately. At the same time, the cost is lower, the operation is simple, and the kit is suitable for being used in grassroots units; and a rapid, accurate and simple detection tool isprovided for laboratory rapid identification and large scale epidemiological investigation of bovine infectious rhinotracheitis virus.

The invention relates to the field of animal bacteriology, and provides an Envision immunohistochemical detection method of Clostridum Perfringens alpha toxin. The Envision immunohistochemical detection with the advantages of high specificity, good repeatability, low background and realization of visual and simple detection of the alpha toxin is established on the basis of a mouse anti-alpha toxin monoclonal antibody. The method can be used for detecting the Clostridum Perfringens alpha toxin in tissues of animals except mice, and provides an effective and reliable way for detection of the distribution of the toxin in the animal bodies.

The invention discloses a portable bacteriological detection system based on a smart phone. A general bacteriological experiment includes lots of complex equipment and essential skills, which greatly hinder the application of microbiology. Based on a portable microbiological medium, the invention describes an instrument which can satisfy the overall process of growth, reservation and sterilization of bacteria, and extremely rigorous bio-safety measures are designed. According to the portable bacteriological detection system disclosed by the invention, the smart phone is used for simultaneously photographing and analyzing a multiple reference sample and a detection sample; if the analysis on a control group of which the results are known is wrong, the analysis of an analysis sample is not started, and thus wrong analysis is prevented, and the scientific preciseness of the analysis is greatly improved. Meanwhile, the system is a scientific researching and analyzing tool which can enable a commonly-educated person to finish scientific experiments under the training of smart phone software and to learn new skills.

This invention relates to a multiple PRC quick test reagent box for pathogens often seen in animal and bird meats and aquatic products including 10xPCR buffer solution, MgCl2, dNTP, primers and DNA poly-enzymes. The tet method includes: picking up a due test sample template, adding 10xPCR buffer solution, MgCl 2, dNTP, primers, DNA poly-enzymes, the due test sample template and ddH20 water to be mixed and enlonged on a PCR instrument, electrophoresis and expansion are made to the product in an electrophoresis device to register the result to be analyzed and judged.

The invention relates to a kit and detecting method for quickly detecting mutiplex PCRs of five common pathogenic bacteria in disposable hygiene products. The kit comprises nuclease-free water, 10*PCR buffer solutions, dNTP, upstream-downstream combined primers, DNA polymerases, negative quality control products and positive quality control products. The kit provided bt the technical scheme of the invention has a favorable detection specificity, and can realize quick detection of salmonella, pseudomonas aeruginosa, staphylococcus aureus, colibacillus and candida albicans, and as compared with conventional bacteriology, the diagnosis is quick, simple, convenient and economy.

The present invention generally relates to the fields of molecular biology, clinical bacteriology and protein folding. More particularly, the invention relates to methods for recombinantly expressing a soluble mature Streptococcus pyogenesexotoxin B (SpeB) polypeptide in a host cell.

The invention relates to the field of animal bacteriology and provides a capture ELISA (enzyme-linked immunosorbent assay) detection method for a Clostridium perfringens Alpha-toxin antibody; in the method, prepared type-A Clostridium perfringens exotoxin is used as an antigen that is used for immunizing a rabbit to obtain rabbit serum and for acting as a binding antigen for capture ELISA after being concentrated and purified; the detection method comprises the steps of (1) enveloping; (2) sealing; (3) adding an antigen; (4) adding a sample to be tested; (5) adding a secondary antibody; (6) developing color; (7) ending; (8) judging results. The detection method has the advantages of high specificity, high sensitivity, good repeatability, low cost and the like; in addition, the detection method is suitable for detecting samples in large batch, and an effective and simple serological diagnostic method is provided for the detection and research on such toxin.

The invention discloses a PCR (polymerase chain reaction) amplification primer for fast detecting mannheimia haemolytica and application thereof, and belongs to the technical field of animal bacteriology and molecular biology. The PCR amplification primer consists of an upstream primer with the nucleotide sequence shown as SEQ ID NO:1 and a downstream primer with the nucleotide sequence shown as SEQ ID NO:2; the primers are used for preparing a PCR amplification kit for fast detecting the mannheimia haemolytica. A PCR detecting method provided by the kit has high specificity, sensibility, highrepeatability and high reliability; the detecting result is fast and accurately obtained; meanwhile, the cost is low; the operation is simple and convenient; the defects of low separation rate, timewaste, labor waste and the like of a traditional identification method are overcome; the PCR amplification primer is suitable for being used for basement layers, and can be used as a fast, accurate, simple and convenient detecting tool for fast detection of mannheimia haemolytica laboratories and survey of large-scale epidemiology.

The invention discloses an industrialized breeding method for sterile cordyceps sinensis host larvae. The industrialized breeding method comprises the following steps of preparing sterile eggs, sterile feed and a sterile breeding device which are prepared in advance, placing the sterile eggs, the sterile feed and the sterile breeding device in a sterile room, detecting no pollution, then turning on a fan, turning on the sterile breeding device, moving the prepared sterile feed into the sterile device by using a sterile tool, placing the sterile eggs on the sterile feed, fastening a top cover of the sterile device, and placing in a sterile production plant area. The industrialized breeding method has the advantages that sterile insects are bred, no outside pathogenic microorganism enters, the survival rate can be increased, and the sterile insects lack horizontally transmitted microorganisms and are weaker in immunocompetence, so that infection of cordyceps sinensis can be facilitated, the method can provide a professional platform for researching the health relationship between microorganisms and the cordyceps sinensis host larvae, including pathogen research of some diseases, antagonism among microorganisms, virus disease research, bacteriology research, fungal infection research and protozoa infection research.

The invention discloses a rabbit spinal tuberculosis animal model building method based on twelfth rib positioning. The method comprises the following steps of randomly selecting a plurality of healthy white rabbits with unlimited male and female and weight of 2.8-3.0kg, randomly selecting one white rabbit, weighing, and performing ear marginal vein anesthesia by using pentobarbital; and fixing the white rabbit anesthetized in the step 1 on an animal operation plate, and then performing a series of operations to complete modeling. According to the rabbit spinal tuberculosis animal model building method based on the twelfth rib positioning, the multiple white rabbits are used for conducting anesthesia in sequence and then conducting an operation, bone parts are punched, tubercle bacillus samples are implanted, then wounds are sewn, cage-separated feeding is conducted, a contrast test is conducted, the states of the white rabbits after the test are observed after the white rabbits are bred for a period of time, and vertebral bodies of rabbits were examined by X-ray, MRI imaging, specimen pathology, bacteriology and the like. The method has the advantages of being easy to locate, lowin cost, practical in technology, short in period, convenient to model, high in success rate and the like, and the basic performance after tubercle bacillus infection can be clearly determined by combining the general situation of animals, the typical imaging performance and the combined bacterium culture situation.

The invention discloses an RT-PCR primer for rapidly detecting Ferlavirus and application of the RT-PCR primer, and belongs to the technical field of animal bacteriology and molecular biology. The RT-PCR primer is composed of an upstream primer having a nucleotide sequence shown in SEQ ID NO: 1 and a downstream primer having the nucleotide sequence shown in SEQ ID NO: 2; and the primer is used toprepare an RT-PCR kit for detecting Ferlavirus. An RT-PCR detection method provided by the kit has high specificity and sensitivity, high repeatability, and high credibility, detection results are quickly and accurately acquired, and the method is low in cost and simple in operation, overcomes low separation rate, time and labor consumption and other deficiencies of a traditional identification method, is suitable for use at a base level, and can be used as a rapid, accurate and simple detection tool for laboratorial rapid detection and large-scale epidemiological investigation of Ferlavirus.