1126 results about "Enterobacter" patented technology

Disclosed herein are various open reading frames from a strain of E. coli responsible for neonatal meningitis (MNEC), and a subset of these that is of particular interest for preparing compositions for immunising against MNEC infections.

In one aspect, the invention provides a purified and modified phytase enzyme from Escherichia coli K12 appA phytase. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In one aspect, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

A method of reducing the content of TSNA in tobacco leaves, comprising treating the tobacco leaves with a microorganism having no nitrate-reducing ability but having the ability of growth-competition with a microorganism belonging to Enterobacter or Pantoea genus.

A method and a composition for treatment of pulmonary bacterial infections caused by gram-negative bacteria suitable for treatment of infection caused by Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Haemophilus influenzae, Proteus mirabilis, Enterobacter species, Serratia marcescens as well as those caused by Burkholderia cepacia, Stenotrophomonas maltophilia, Alcaligenes xylosoxidans, and multidrug resistant Pseudomonas aeruginosa, using a concentrated formulation of aztreonam, or a pharmaceutically acceptable salt thereof, delivered as an aerosol or dry powder formulation.

The invention discloses a multiplex LAMP detection primer, kit and method for six food-borne pathogenic bacteria in fruits and vegetables and belongs to the technical field of bacterial gene detection. A rapid detection primer set for the six pathogenic bacteria including listeria monocytogenes, enterobacter sakazakii, shigella spp, staphylococcus aureus, salmonella spp and escherichia coli O157:H7 is designed, and multiplex LAMP reaction is performed on the genome DNA of the bacteria extracted from a sample to be detected in the same reaction system by use of the detection kit including the primer set to determine whether the sample contains the six food-borne pathogenic bacteria or not. The multiplex LAMP detection primer is high in specificity and sensitivity and can accurately detect the genome DNA of the six food-borne pathogenic bacteria in the same reaction system, can realize simple and convenient, quick and accurate detection, is suitable for on-site rapid detection and has significance on improving the pathogenic bacterium analysis and detection technology and the fruit and vegetable edible quality security.

The invention relates to enterobacter ludwigii BG10-1 and application thereof in biological prevention and control over aspergillus flavus. The enterobacter ludwigii BG10-1 is preserved in China Center for Type Culture Collection (CCTCC) on January 7, 2016, the preservation number is CCTCC NO: M 2016014, and the classification name is EnterobacterLudwiggi BG10-1. The enterobacter ludwigii BG10-1 is obtained by being separated from peanut pods produced in Babu Town, Hezhou City, Guangxi Province. Through indoor confrontation and antagonism experiments, living body bacteriostasis and detoxification experiments and field prevention and control experiments, it is found that the strain is high in bacteriostatic capacity on the aspergillus flavus, easy to culture, free of contamination and safe to environment. By applying microbial fertilizer of the enterobacter ludwigii BG10-1, contamination of the aspergillus flavus to peanuts can be effectively prevented and controlled.

The invention discloses a double-antibody sandwich method for detecting salmonella typhimurium in food based on monoclonal antibodies, belonging to the technical field of immunoassay. Salmonella typhimurium ATCC13311 and smooth salmonella typhimurium LPS are adopted for mixed immunity of a 7-week BALB / c mouse, 10 LPS monoclonal antibodies are obtained by immunity, fusion and screening, horse radish peroxidases (HRP) are labeled respectively, and the salmonella typhimurium is paired two by two. A sandwich enzyme-linked immuno sorbent assay (ELISA) method is established by taking 6E2 CGMCC No.7206 monoclonal antibodies as coated antibodies and enzyme-labeled antibodies and by taking the salmonella typhimurium as standards, and the LOD is 500cfu / mL. The sandwich method, established by using the monoclonal antibodies which are highly uniform in physicochemical property and high in specificity and can be prepared on a large scale, is high in sensitivity and low in cost; the salmonella typhimurium is not in cross reaction with salmonella enteritidis, salmonella arizonae, E.coli, E.coliO157:H7, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes; a quick and efficient analysis way is provided for detection of the salmonella typhimurium in the food.

The invention provides a nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip. The nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane immobilized with streptavidin, and an absorbent pad; the nitrocellulose membrane is provided with a detection line and a quality control line; the sample pad, the colloidal gold combined pad, the nitrocellulose membrane, and the absorbent pad are glued onto a plastic liner board successively; colloidal gold labeled nucleic acid aptamer is arranged on the colloidal gold combined pad; a capture probe is immobilized on the detection line; and a quality control probe is immobilized on the quality control line. The invention also provides a method used for detecting escherichia coli O157:H7 with the nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip. According to the nucleic acid aptamer-based escherichia coli O157:H7 colloidal gold test strip, the escherichia coli O157:H7 outer membrane protein high specificity aptamer is taken as a recognition element of test strip instead of antibodies, so that problems of immunochromatographic test strip containing antibodies, such as high cost, large batch difference, induced cross reaction, and low sensitivity, are solved, and high-efficiency rapid detection of escherichia coli is realized.