152results about How to "To achieve the detection effect" patented technology

The invention discloses a fourteen-food-borne pathogenic bacterium multiplex PCR detection primer set and a kit. The detection primer set is composed of primer pairs for detecting salmonella, Shigella, Vibrio parahemolyticus, campylobacter jejuni, campylobacter coli, staphylococcus aureus, bacillus cereus, Listeria monocytogenes, yersinia enterocolitica, enterobacter sakazakii, Escherichia coli, vibrio cholerae, Escherichia coli O157, aeromonas hydrophila and internal positive control. A multiplex PCR detection method based on an ordinary PCR platform is built, multiplex PCR reactions are carried out on genomic DNA, extracted from a sample to be tested, of bacteria in the same reaction system through the primer pairs acquired through analysis and design, and whether the food-borne pathogenic bacteria are contained in the sample or not is judged through the electrophoretic analysis of reaction products.

The invention provides a flexible pulse sensor and a manufacturing method thereof. The flexible pulse sensor comprises a flexible base, carbon nano tube-graphene composite film of which part is embedded into the flexible base, and electrodes, wherein the electrodes are arranged at two ends of the carbon nano tube-graphene composite film; the thickness of the flexible base is 0.8-1.2mm; the light transmittance of the initial carbon nano tube is 85-95%, and the light transmittance of the carbon nano tube-graphene composite film obtained through chemical vapor deposition is 80-92%. According to the flexible pulse sensor and the manufacturing method thereof, the carbon nano tube, the graphene and the flexible base synergize with one another so that the flexible pulse sensor can not only have the advantages of the graphene and the carbon nano tube sensor but also avoid the disadvantages of the graphene and the carbon nano tube sensor, the mechanical stability of a strain sensor is strengthened, the pulse sensor does not lose efficacy within at least 10% of strain ranges, the pulse sensor can effectively catch pulse signals, and the detecting effect is reached.

The invention discloses a visible light and infrared detection result integration based moving target detection method which is used for solving the technical problem that the existing moving target detection method is poor in detection effect. According to the technical scheme, the visible light and infrared detection result integration based moving target detection method comprises extracting a foreground picture of a visible light image by a ViBe background extraction method; converting the foreground picture to an LUV space for shadow judgment after the foreground picture is obtained; extracting a significant figure of an infrared image by a significance detection method; integrating two detection results and extracting a final moving target. According to the visible light and infrared detection result integration based moving target detection method, the technical problem of the poor target detection effect caused by shadows and illumination intensity environment changes can be effectively solved and the all-weather detection effect can be achieved.

The invention discloses a vortex line scanning thermal imaging detection system and method. The detection system comprises a trigger with at least two output ends, one output end of the trigger is connected with a coil through a vortex flow heating module, other output ends of the trigger are respectively connected with one or more thermal imaging acquisition devices, each thermal imaging acquisition device is equipped with a lens used for adjusting the size of a viewing field, the output end of each thermal imaging acquisition device is connected with a computer, the vortex line scanning thermal imaging detection system further comprises a scanning control module connected with the output end of the computer, the output end of the scanning control module is connected with a motion device, a clamping device is further arranged on the motion device, the clamping device is respectively and fixedly connected with the coil and each thermal imaging acquisition device, or the clamping device is fixedly connected with a detected object. Compared with the prior art, the vortex line scanning thermal imaging detection system provided by the invention is convenient to use, high in detection efficiency, has a good imaging effect, high in defect detecting accuracy, low in detecting cost, and high in detecting result reliability.

The invention relates to a ligand for aggregation-induced emission and metal complexes thereof, belonging to the field of biological detection. The ligand simultaneously comprises a macrocyclic amine group and a group for aggregation-induced emission (AIE), wherein both the groups are covalently linked or bridged through common organic units. According to different metal ions chelated in the ligand, the complexes show different functions, wherein the complexes of zinc, cadmium and the like can be used as anion fluorescent probes and applied in detection of molecules like nucleic acid and protein; the complexes of lanthanide metals can be applied in fluorescent and phosphorescent detection and imaging for time resolution; and the complexes of paramagnetic metals can be applied in magnetic resonance imaging.

The invention relates to a tin soldering machine abnormal tin-discharging monitoring method which includes the steps: enabling tin wires and a soldering mouth to be insulated before tin is charged, continuously detecting if the tin wires are conducted with the soldering mouth between start of tin-charging and stop of the tin-charging, and judging detecting results after the tin-charging is stopped. If conduction is achieved, the tin-charging is judged to be normal, otherwise the tin-charging is abnormal. The invention discloses a tin soldering machine abnormal tin-discharging monitoring mechanism which is provided with an insulation mechanism, a detecting mechanism and a judging mechanism, wherein the insulation mechanism is used for insulating the tin wires and the soldering mouth and the detecting mechanism is used for detecting if the tin wires are conducted with the soldering mouth. The invention further relates to a tin-discharging device and a tin soldering machine, the tin-discharging device and the tin soldering machine are with the abnormal tin-discharging monitoring method and the abnormal tin-discharging monitoring mechanism. The tin-discharging device and the tin soldering machine are suitable for being applied in the tin wires with various types, and capable of judging if the tin wires are sent to the soldering mouth and if tin-blocking happens. Due to the fact that as long as the tin-blocking happens at any position of the tin-discharging device, the tin wires can not be sent to the head portion of the soldering mouth, and a non-detection zone not exists. The tin-blocking can be detected. The tin-discharging device and the tin soldering machine are good in instantaneity, high in susceptiveness and low in cost.

The invention, which belongs to the field of photoelectrochemical visualization biosensor construction, relates to preparation of a photoelectrochromic visualization biosensor based on the ratio principle. A region 1 and a region 2 of two electrodes being a module A and a module B respectively are modified respectively by using Prussian blue and a Co-N-TiO2 / 3DGH nanocomposite material; the surfaceof region 2 is processed by using chitosan; the surface of region 2 of the module A is modified with an ochratoxin A adaptor solution, incubation is carried out, and washing is carried out by using aphosphate buffer solution; an active site that is not bonded is sealed by using bovine serum albumin; and then washing is carried out by using a phosphate buffer solution to obtain a photoelectrochromic visualization biosensor based on the ratio principle. The Co-N-TiO2 / 3DGH nanocomposite material represents the high photoelectric activity and stability; and with the constructed Co-N-TiO2 / 3DGH nanocomposite material, the interferences of various external and internal factors are reduced substantially and the detection accuracy is improved. Moreover, no complicated instrument is needed and naked-eye identification is used, so that the visual inspection is realized.

The invention discloses a power battery environment bin and belongs to the technical field of battery testing devices. The power battery environment bin comprises a bin body, a refrigerating unit anda water cooler, a bin door is arranged on the left side of the bin body, and an air exhaust system, a fire-extinguishing system and a poisonous gas detecting system are sequentially arranged at the top of the bin body from left to right. A battery carrying sub-master vehicle is moved by means of the method that a master plate is equipped with first trundles, the surface of the master plate and theinterior of the bin body are provided with a supporting guiding rail and a bearing guiding rail correspondingly, and by means of second trundles, a sub plate can move to the bearing guiding rail in the bin body from the supporting guiding rail through the supporting plate. The supporting plate is rotatably connected with a U-shaped block, the supporting plate is stored through a containing groove, a spring and a connecting block, and a cushion plate is clamped at the edge of the containing groove, so that taking-out is convenient. According to the power battery environment bin, battery burning or exploding in the detection testing process can be detected in time, safe operation is conducted through air exhaust and fire-extinguishing devices, meanwhile carrying is conducted through the battery carrying sub-master vehicle, fixing of a battery is facilitated, and the work efficiency and testing accuracy are improved.

The invention provides a multifunctional low-dimensional photoelectric material detection platform, effectively solving problems in the prior art. The detection platform comprises a base and is characterized in that a first clamping hand is mounted on the top surface of the base, a rack groove is formed in the right end of the top surface of the base, an arc-shaped rack is slidably arranged in therack groove, a second clamping hand is mounted on the arc-shaped rack, a rotary rod is mounted on the inner bottom surface of the base, a driving gear is mounted at the top of the rotary rod, a driven toothed disc located below the driving gear is mounted on the rotary rod, a sleeve sheathes the rotary rod, the top of the sleeve is connected with a transmission toothed disc, the bottom of the sleeve is connected with a driving toothed disc, and the transmission toothed disc is connected with a motor. The detection platform provided by the invention realizes stretching and bending on a solar cell panel by virtue of the first clamping hand which can move left and right and a second clamping hand on which the arc-shaped rack is mounted as well as a pressure bar, so that the solar cell panelonly bends at the pressure bar and can not be stretched; and by virtue of the sleeve which moves up and down, different detection effects can be achieved, and solar cell panel strength detection efficiency is improved.

The invention discloses a Shigella flora / serotype multiplex-PCR (polymerase chain reaction) detection primer set and kit. The detection primer set is composed of primer pairs for detecting Shigella sonnei, type-1-5 Shigella flexneri, type-I Shigella dysenteriae, type-6 Shigella flexneri, and Shigella. According to the invention, a multiplex-PCR detection method based on a common PCR platform is built; according to the method, multiplex-PCR reaction is carried out on total DNA of bacteria extracted from a sample to be detected in a same reaction system by using the primer pairs obtained through analysis and design, and through electrophoretic analysis on a reaction product, whether the sample is Shigella can be determined and Shigella flora / serotype can be judged.

The invention discloses a method and a device for detecting web page updating. The method includes: acquiring the historical access records of a to-be-detected website; determining the historically accessed web pages in the to-be-detected website according to the historical access records; acquiring the current access record of the to-be-detected website; acquiring the currently accessed web page in the to-be-detected website according to the current access record; judging whether the currently accessed web page is in the historically accessed web pages or not; if so, determining that the currently accessed web page is not a newly added web page; if not, determining that the current access record is a newly added web page. By the method, the problem that web page updating condition detecting is high in load consumption is solved, whether the current web page is a newly added web page or not is determined by the historical access records of the website, and the web page updating conditions can be detected without causing high load consumption.

The invention provides a primer group for detecting diarrhea-causing parasite genes through the multi-PCR technology. The primer group comprises primers shown as SEQ ID NO.1 and SEQ ID 3-18. The invention further provides a detection method and a kit for detecting the diarrhea-causing parasite genes through the multi-PCR technology. After the technical scheme is adopted, a complete technical means is provided for the rapid and accurate screening of the diarrhea-causing parasite genes, the rapid screening and identification for the diarrhea-causing parasites after the food poisoning event are realized, and a reliable basis is provided for reasonably and properly disposing the diseases.

The invention provides a primer set for detecting bacillus cereus virulence genes by means of a multiplex-PCR. The primer set comprises primers shown in SEQ ID NO.1 and 3-24. The invention further provides a detection method and kit for detecting bacillus cereus virulence genes by means of the multiplex-PCR. According to the technical scheme, the perfect technological means is provided for rapid and accurate screening of the bacillus cereus virulence genes, rapid screening and identification of pathogenicity bacillus cereus after a food poisoning event and outbreak of an epidemic situation can be achieved, and the reliable basis is provided for reasonable and appropriate epidemic treatment.

The invention provides a consumption signal conversion module which comprises a drive signal generation circuit, a detection circuit, a modem circuit and a pulse signal generation circuit which are integrated in a module by a thick module. An RC bridge type sine wave oscillation circuit of a power amplifying circuit is connected in series in the drive signal generation circuit, a switch K4 and a capacitor C15 are arranged at the control end, the RC bridge type sine wave oscillation circuit generates two alternating current drive signals through the power amplifying circuit, the drive signals drive two ends of a consumption sensor through a positive phase voltage following circuit and a negative phase voltage following circuit to enable the consumption sensor to form a modulation carrier signal in the rotating process, the modulation carrier signal is filtered, detected, amplified and compared through the modem circuit and then transferred to the pulse signal generation circuit to generate a pulse signal for a micro processor to recognize. The consumption signal conversion module is small in size, low in power consumption and high in reliability, overcomes the shortcoming in the prior art that a printed circuit board (PCB) board occupies a large amount of assembly space, and can detect fault information of the consumption sensor on an airplane.

The present disclosure discloses a primer probe set and a kit using recombinase polymerase amplifying for detecting Marburg virus and a detection method. The primer probe set includes primers with nucleotide sequences shown in SEQ ID NO. 1 to 2 and a probe with a nucleotide sequence shown in SEQ ID NO.3. The present disclosure also provides the kit using recombinase polymerase amplifying for detecting the Marburg virus. The kit includes the primer probe set. The primer probe set and the kit significantly improve the sensitivity, specificity, and simplicity of the detection of the Marburg virus.

The invention discloses a false comment detection method based on a double-cycle graph, comprising the following steps: (1) using an original graph filter to calculate a comment confidence degree anda user confidence degree of the original comment data, and screening the user confidence degree to obtain a reliable user; (2) calculating the store confidence level of the comment data correspondingto the reliable users by using the original graph filter; (3) updating the comment confidence level in the original graph filter to the comment confidence level obtained in the step (1), and calculating the user confidence level of the original comment data by using the original graph filter; (4) taking the store confidence obtained in the step (2) and the user confidence obtained in the step (3)as initial values to construct a weighted map filter; (5) calculating The store confidence, user confidence and comment confidence of the original comment data by using the weighted graph filter, andthe false comment is obtained by screening according to the comment confidence. This method improves the detection accuracy of false comments.

The invention provides real-time fluorescence quantitative PCR (polymerase chain reaction) primers and probes for detecting vomit-type Bacillus cereus, and a method for detecting the vomit-type Bacillus cereus by using the primers and probes. The nucleotide sequences of the primers and probes are respectively disclosed as SEQ ID NO.3 through SEQ ID NO.8. The invention also provides a kit for detecting the vomit-type Bacillus cereus. The detection method and kit thereof have the advantages of high detection accuracy, high sensitivity and specificity, are simple, convenient and quick, and have favorable clinical specimen detection capability.

The invention discloses a communication data detection method and device. The communication data detection method comprises the steps that communication data transmitted by a communication protocol to be detected are acquired; a configuration file corresponding to the communication protocol to be detected is called, wherein the configuration file is a preconfigured file of data stored according to a preset format for the communication protocol to be detected, and the preset format is the inherent format of the configuration file; the acquired communication data are detected through the configuration file. According to the communication data detection method and device, the problem that in the prior art, different detection software programs need to be written for different communication protocols is solved, and the effect that the same detection software can be adopted for detection on communication data transmitted by different communication protocols is achieved.

The invention relates to an enhanced highlight LED (light-emitting diode) flashlight capable of detecting a high-power concentrator cell, belonging to the technical field of solar photovoltaic power generation. The enhanced highlight LED flashlight comprises a reflector a switch button, a hook, a charging port, an anti-skidding groove, a highlight LED lamp, a flashlight body, a PCB (printed circuit board) board and a threaded condensation shield, and is characterized in that the highlight LED is mounted on the PCB board and is fixed in a lamp holder, the lamp holder is internally equipped with the reflector, the threaded condensation shield is installed on the lamp holder, the hook and the charging port are installed on the tail part of the flashlight body, the switch button is designed a push mode, the anti-skidding groove is carved in a handle of the enhanced highlight LED flashlight, and a rechargeable lithium battery is arranged in the flashlight to prolong the life of the LED lamp to be 20,000 hours. The enhanced highlight LED flashlight is convenient to maintain and carry, low in maintenance cost and small in size, can effectively utilize the light source under the help of condensation shield and is applied to field overhaul of a high-power condensation battery module.

The present disclosure relates to a nucleic acid reagent, kit and system for detecting enteroviruses. The nucleic acid reagent comprises primers shown in SEQ ID NO.1-36 and probes shown in SEQ ID NO.39-58, wherein the primers and probes are stored separately from each other or arbitrarily mixed with each other. The nucleic acid reagent, kit and system detecting 22 pathogenic enteroviruses are established by using the above primers and probes, and can realize rapid, comprehensive, sensitive, specific and automatic detection result determination, and significantly improve sensitivity, specificity, and simplicity of simultaneous detection of multiple enteroviruses.

The invention relates to a nucleic acid reagent, a kit, a system and a method for detecting Gram-negative bacteria and / or drug resistance of Gram-negative bacteria. The nucleic acid reagent comprisesprimers shown as the SEQ ID NO. 1-60 and probes shown as the SEQ ID NO. 63-92 which are independently stored or stored as arbitrary mixtures. By using the primers and the probes, the nucleic acid reagent, the kit, the system and the method for detecting 6 pathogens, including Klebsiella pneumoniae, Escherichia coli, pseudomonas aeruginosa, enterobacter cloacae, serratia marcescens and acinetobacter baumannii, as well as 24 common drug resistance genes carried by the 6 pathogens are established by the invention; moreover, fast, comprehensive, sensitive, specific and automatic detection result determination can be realized, so that, sensitivity, specificity and simplicity of simultaneous detection of multiple Gram-negative bacteria and drug resistance thereof are significantly improved.

The invention relates to the field of detecting a moving target by a narrow-band ground-based radar in a clutter environment, and particularly relates to a method for detecting a low signal-to-clutterratio (SCR) moving target by a stepped frequency coherent processing. The method comprises the following steps: transmitting an inter-pulse same frequency signal by a radar with the frequency stepping between pulse group signals, firstly performing equal order AMTI on each carrier frequency pulse group signal in a group in the received signal processing; performing step frequency phase synthesisafter phase compensation on a time-aligned AMTI result of each carrier frequency; and finally completing coherent accumulation by a step frequency synthesized image at a slow beat; thereby completingseparation between the moving target and the remaining clutter, and achieving a purpose of improving the SCNR of the moving target detected.

The invention discloses an environmentally friendly purifying device with a circulating filtration function. The purifying device comprises a box. Two sides of the bottom of the box are both fixedly connected to support legs. The tops of two sides of the inner cavity of the box are both fixedly connected to fixing blocks. The sides, which are far away from the box, of the fixing blocks are fixedlyconnected to a filter box. A filter case is arranged in the inner cavity of the filter box. By setting the filter case, a filter cotton layer, and an active carbon adsorption layer, the sewage is filtered gradually; the purified water can be stored in a water tank; through a water quality detector, a water pump, and a long pipe, the quality of purified water is detected; the disqualified water isfiltered in cycle; and solved are the problems that in the prior art, the sewage cannot be filtered in cycle, processed sewage still contains a large amount of harmful substances, and the use performance of a sewage processing device is largely reduced.

The invention discloses a printing machine based on a blockchain and relates to the technical field of printing machines. The printing machine comprises a base; a box body is fixedly mounted at the top of the base; a display lamp and a display screen are fixedly mounted on the front surface of the box body sequentially from left to right; a motor and a casing are fixedly mounted at the top of an inner cavity of the box body sequentially from left to right; fan blades are fixedly mounted on an output shaft of the motor; supporting rods are fixedly mounted on two sides of the bottom of the inner cavity of the box body; and the top ends of the two supporting rods are fixedly connected through a plate body. According to the printing machine, through improvement of the casing, under the effects of a sound insulating net, an inserting rod, a sound insulating plate, a reset spring, a transverse plate, a sponge block, a sliding rail, a sliding rod and a cotton yarn net, and the effect of noise reduction of the printing machine is realized, so that noise pollution of the printing machine is reduced, and the use efficiency of the printing machine is improved while the use comfort is improved.

The invention relates to a nucleic acid reagent, reagent kit, system and method for detecting escherichia coli and klebsiella pneumoniae and toxicity and reverse tolerance of the escherichia coli andthe klebsiella pneumoniae. The nucleic acid reagent comprises a primer as shown in SEQIDNO.1-26 and a probe as shown in SEQIDNO.29-41, which are in mutual independent storage or mutual discretionary mixed storage. Through the primer and the probe disclosed by the invention, the nucleic acid reagent, reagent kit, system and method for detecting the escherichia coli and klebsiella pneumoniae and 6 virulence factors and 5 reverse tolerance genes, carried by the escherichia coli and the klebsiella pneumoniae, are constructed, quick, comprehensive, sensitive, excellent and automatic testing resultjudgment can be realized, and the sensibility, the specificity and the simplicity for detection for target detection genes can be notably improved.

The invention discloses a database index processing method and device. The database index processing method comprises the steps of obtaining the annotation information of pre-added indexes, wherein the annotation information is used for recording the original format used by the indexes; carrying out analysis on the annotation information to obtain the original format used by the indexes and recorded by the annotation information, obtaining metadata of the indexes; carrying out comparison on the original format used by the indexes recorded by the annotation information and the metadata of the indexes; obtaining the using information of the indexes according to comparison results. Through the database index processing method and device, the effect of carrying out detection on the using situation of the indexes is achieved, and maintenance on the indexes is convenient to carry out.

The invention relates to the technical field of detecting and packaging devices, and discloses a puffed food detecting and packaging device which comprises a base connecting assembly and an extrudingassembly. Fixing columns are fixedly mounted on the left side and the right side of the top end of the base correspondingly, and an air pump is fixedly mounted on the inner wall of the fixing column located on the right side. A moving plate moves downwards to drive the extruding assembly to move downwards, a to-be-detected object can provide counter-acting force for the extruding assembly in the downward moving process, a multi-section telescopic rod can contract, air can be blocked by a connecting rod, if air leakage happens to the to-be-detected object, the multi-section telescopic rod cannot bear extrusion force, therefore, the connecting rod and an air blocking plug cannot block air, the air in the moving plate can enter the fixing column on the left side and push a sealing head and amoving block to move rightwards, a constant-pressure rod contracts, a pushing block also moves rightwards along with the sealing head and the moving block, unqualified articles are pushed out of the base, and the effect of packaging and detecting puffed food is further achieved.

The invention relates to the technical field of drug detection, and discloses a preparation method and applications of an aggregation-induced emission probe material. The preparation method comprises:1) dissolving carboxylated oligomeric silsesquioxane in N,N-dimethylformamide; 2) adding 4-dimethylaminopyridine, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and triethylamine, and stirring in an ice water bath; 3) adding 1-hydroxypentaphenylsilole or 1-aminotetraphenylethylene, carrying out a thermal insulation reaction, adding to distilled water, and stirring to obtain a white precipitate; and 4) carrying out suction filtration, and washing to obtain the aggregation-induced emission probe material. According to the present invention, the aggregation-induced emission probe material is not affected by the external conditions in drug detection, and further has advantages of simple detection and short detection time.

Provided in the invention is a two-wheel-driving type low-speed all-weather unmanned cruiser system comprising a cruiser body and a control system. The cruiser body has driving wheels and driven wheels; and one pair of driving wheels are driven by two direct-current motors connected with planetary gears. Each of a first front environmental detection and protection system and a first rear environmental detection and protection system in the control system is composed of a single-line laser radar, a single-line laser radar and a CCD camera; each of a second front environmental detection and protection system and a second rear environmental detection and protection system consists of two microwave radars; and each of a front blind zone detection and obstacle avoidance system and a rear blindzone detection and obstacle avoidance system includes three ultrasonic sensors. The single-line laser radars, the microwave radars, the CCD cameras, a wireless communication module, a wireless communication module and an auxiliary controller are connected with a main controller; the ultrasonic sensors and the motor drivers are connected with an auxiliary controller; and motor drivers are connectedwith the two direct-current motors. The system has high stability, reliability and cost performance, fast operation processing speed, high flexibility, and effective coping with bad weather.

The invention discloses an adjustable finger clip blood oxygen tester, which comprises a first fixing plate, a first groove is opened on the right side of the top of the first fixing plate, and a first groove is opened on the left side of the top of the first fixing plate. A fixed block is fixedly connected, and the fixed block is movably connected with a second fixed plate through a rotating shaft. The right side of the surface of the second fixed plate is provided with a second groove, and the left side of the inner cavity of the second groove is provided with Drive gear. The present invention achieves the effect of placing the user's finger and detecting the user by setting the first fixing plate, the first groove and the second fixing plate, and achieves the effect of driving the threaded rod to rotate through the driving gear and the driven gear. The threaded rod and the threaded tube achieve the effect of driving the movement of the splint. Through the splint, the effect of fixing the user's finger is achieved, which can effectively fix the user's finger. During the detection, the finger is not easy to fall off, which ensures the accuracy of the detection. Effect.