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Photoelectrochromic visualization biosensor based on ratio principle and preparation method and application thereof

A biosensor, color-changing technology, applied in the direction of analysis, instruments, scientific instruments, etc., by causing chemical reactions to occur in materials, can solve the problems of long processing time, high cost, complex equipment requirements for operation technology, etc., and achieve simple and effective detection. Effect

Active Publication Date: 2019-11-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional OTA detection methods, including thin-layer chromatography (TLC), enzyme-linked immunoassay (ELISA), and liquid chromatography-tandem mass spectrometry, are costly, have long processing times, and require certain operational techniques and complex equipment requirements

Method used

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  • Photoelectrochromic visualization biosensor based on ratio principle and preparation method and application thereof
  • Photoelectrochromic visualization biosensor based on ratio principle and preparation method and application thereof
  • Photoelectrochromic visualization biosensor based on ratio principle and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Co-N-TiO 2 / Preparation of 3DGH nanocomposites and Prussian blue PB solution

[0034] First, cobalt-nitrogen co-doped titania nanoparticles were first prepared as follows: solution A was to place 30 mL of ethanol in a 250 mL round bottom flask and stir. Then, 2.0 g of urea was added as a nitrogen source. In addition, 0.05 g of cobalt nitrate was added to the round bottom flask. Finally, 2 mL of ultrapure water was added thereto. Solution B is to add 30mL ethanol and 3mL HNO respectively to another 250mL round bottom flask 3 . Finally, 10 mL of TBT was slowly added to the above solution and stirred for half an hour. Solution B was added dropwise to solution A at a rate of 1 mL / min, and then stirred for 120 min. After the mixed solution was stirred, it was transferred to a 100 mL autoclave and heated in an oven at 120° C. for 16 h. Then it was washed several times with ultrapure water and dried overnight in an oven at 100 °C. Finally, the obtained sample was p...

Embodiment 2

[0043] 1) Co-N-TiO 2 / Preparation of 3DGH nanocomposites and Prussian blue PB solution

[0044] First, cobalt-nitrogen co-doped titania nanoparticles were first prepared as follows: solution A was to place 30 mL of ethanol in a 250 mL round bottom flask and stir. Then, 2.0 g of urea was added as a nitrogen source. In addition, 0.05 g of cobalt nitrate was added to the round bottom flask. Finally, 2 mL of ultrapure water was added thereto. Solution B is to add 30mL ethanol and 3mLHNO respectively to another 250mL round bottom flask 3 . Finally, TBT was slowly added to the above solution and stirred for half an hour. Solution B was added dropwise to solution A at a rate of 1 mL / min, and then stirred for 120 min. After the mixed solution was stirred, it was transferred to a 100 mL autoclave and heated in an oven at 120° C. for 16 h. Then it was washed several times with ultrapure water and dried overnight in an oven at 100 °C. Finally, the obtained sample was placed in a...

Embodiment 3

[0053] 1) Co-N-TiO 2 / Preparation of 3DGH nanocomposites and Prussian blue PB solution

[0054] First, cobalt-nitrogen co-doped titania nanoparticles were first prepared as follows: solution A was to place 30 mL of ethanol in a 250 mL round bottom flask and stir. Then, 2.0 g of urea was added as a nitrogen source. In addition, 0.05 g of cobalt nitrate was added to the round bottom flask. Finally, 2 mL of ultrapure water was added thereto. Solution B is to add 30mL ethanol and 3mLHNO respectively to another 250mL round bottom flask 3 . Finally, TBT was slowly added to the above solution and stirred for half an hour. Solution B was added dropwise to solution A at a rate of 1 mL / min, and then stirred for 120 min. After the mixed solution was stirred, it was transferred to a 100 mL autoclave and heated in an oven at 120° C. for 16 h. Then it was washed several times with ultrapure water and dried overnight in an oven at 100 °C. Finally, the obtained sample was placed in a...

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Abstract

The invention, which belongs to the field of photoelectrochemical visualization biosensor construction, relates to preparation of a photoelectrochromic visualization biosensor based on the ratio principle. A region 1 and a region 2 of two electrodes being a module A and a module B respectively are modified respectively by using Prussian blue and a Co-N-TiO2 / 3DGH nanocomposite material; the surfaceof region 2 is processed by using chitosan; the surface of region 2 of the module A is modified with an ochratoxin A adaptor solution, incubation is carried out, and washing is carried out by using aphosphate buffer solution; an active site that is not bonded is sealed by using bovine serum albumin; and then washing is carried out by using a phosphate buffer solution to obtain a photoelectrochromic visualization biosensor based on the ratio principle. The Co-N-TiO2 / 3DGH nanocomposite material represents the high photoelectric activity and stability; and with the constructed Co-N-TiO2 / 3DGH nanocomposite material, the interferences of various external and internal factors are reduced substantially and the detection accuracy is improved. Moreover, no complicated instrument is needed and naked-eye identification is used, so that the visual inspection is realized.

Description

technical field [0001] The invention belongs to the construction field of photoelectrochemical biosensors, and relates to a ratio photochromic visualized biosensing method with high sensitivity and high selectivity. Background technique [0002] As we all know, photoelectrochemical PEC reflects the electrochemical behavior of photoactive materials under light. Based on this principle, people combine the relationship between the photoelectric response changes produced by the physical and chemical interactions between the analyte and the PEC active material to quantify the concentration of the analyte, which is called photoelectrochemical analysis. Because this analytical method is an analytical technique based on traditional electrochemistry, it has high sensitivity and simple equipment; light is used as the excitation signal and electrical signal is used as the detection signal; the background signal is low and the detection limit is low; it is widely used in the biological ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N21/78G01N21/31
CPCG01N21/31G01N21/78G01N27/416
Inventor 郝楠花荣王坤
Owner JIANGSU UNIV
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