The invention discloses a primer, a probe and a kit for evaluating skin anti-allergy condition. The sequences of the primer and the probe are SEQ ID NO:1-4, SEQ ID NO:5-8, SEQ ID NO:9-12, SEQ ID NO:13-16, SEQ ID NO:17-20, SEQ ID NO:21-24, SEQ ID NO:25-28, SEQ ID NO:29-32, and SEQ ID NO:33-36, wherein the 5' terminals and 3'terminals of SEQ ID NO:3-4, 7-8, 11-12, 15-16, 19-20, 23-24, 27-28, 31-32 and 35-36 are respectively connected with different fluorescent markers. The invention also discloses a method using gene detection to assist a customer to select correct face-beautifying methods. The primer and the probe have the advantage that by acknowledging the internal factors and external factors of skin allergy, a personalized face-beautifying and health solution is provided, and the effect of an anti-allergy face-beautifying product is maximized.