206 results about "Enterobacter cloacae" patented technology

It has been discovered that fluids viscosified with viscoelastic surfactants (VESs) may have their viscosities reduced (gels broken) by the direct or indirect action of a biochemical agent, such as bacteria, fungi, and/or enzymes. The biochemical agent may directly attack the VES itself, or some other component in the fluid that produces a by-product that then causes viscosity reduction. The biochemical agent may disaggregate or otherwise attack the micellar structure of the VES-gelled fluid. The biochemical agent may produce an enzyme that reduces viscosity by one of these mechanisms. A single biochemical agent may operate simultaneously by two different mechanisms, such as by degrading the VES directly, as well as another component, such as a glycol, the latter mechanism in turn producing a by-product (e.g. an alcohol) that causes viscosity reduction. Alternatively, two or more different biochemical agents may be used simultaneously. In a specific, non-limiting instance, a brine fluid gelled with an amine oxide surfactant can have its viscosity broken with bacteria such as Enterobacter cloacae, Pseudomonas fluorescens, Pseudomonas aeruginosa, and the like.

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The invention belongs to the technical field of environment protection and discloses a compound microbial bacterial preparation and application thereof. The compound microbial bacterial preparation consists of fermentation liquor of four bacteria, i.e. Bacillus licheniformis, Pseudomonas putida, Enterobacter cloacae and Candida valida. The compound microbial bacterial preparation provided by the invention has strong digestion power for various types of cellulose, proteins and fat macromolecules. The addition of the compound microbial bacterial preparation into excess sludge and/or kitchen waste can quicken enrichment of methanogen, shorten anaerobic digestion time and improve methane yield. Moreover, the synergetic effect of the four microbial strains is superior to the effect of any one, two or three strains of microorganisms.

The invention relates to a micro-capsulated suspended microbial seed coating agent and a preparation method thereof. The micro-capsulated suspended microbial seed coating agent comprises a functional bacteria microcapsule and auxiliaries, wherein the functional bacteria microcapsule is prepared from wall materials such as sodium alginate and gelatin by virtue of an endogenous emulsion process, the grain size of the microcapsule is 20-60 mu m, the content of functional bacteria is not lower than 10<9>cfu/g, and the embedding rate is over 80%. Functional microbes are selected from two or more of pseudomonas, bacillus subtilis, bacillus amyloliquefaciens, enterobacter cloacae, raoultella planticola and bacillus atrophaeus. The auxiliaries comprise one or more of a film-forming agent, a dispersant, a tackifier, an anti-freezing agent and a coloring agent. The prepared functional bacteria microcapsule is uniformly mixed with the auxiliaries to obtain a finished product. The product has the advantages of good film-forming performances, thallus persistence, high survivability, good slow-controlled release, strong application stability, environment friendliness, convenience in use, and the like, can be used for promoting seed germination and seedling growth of cottons, soya beans, wheat and potatoes, and the like, controlling the diseases at the seedling stage, improving stress resistance, and the like.

The invention discloses a gene chip for detecting ten types of pathogenic bacteria in sea areas. The gene chip comprises a solid phase slide on which a quality control probe and a detection probe are arranged, wherein the nucleotide sequence components of the detection probe are shown in SEQ ID NO.1-SEQ ID NO.27. The gene chip is capable of simultaneously detecting enterobacter cloacae, edwardsiella tarda, streptococcus faecalis, vibrio fortis, vibrio harveyi, vibrio parahaemolyticus, vibrio splendidus, vibrio vulnificus, vibrio anguillarum and shewanella smarisflavi just in the presence of DNA (deoxyribose Nucleic Acid) without need of live bacteria. The gene chip has remarkable advantages of high throughput, parallelism, miniaturization, automation, rapidness, sensitiveness, quantification, small use amount, and the like.

The invention discloses a high efficiency turning glycosyl beta-galactosidase gene. The genes are gained by enterobacter cloacae B5 CGMCC No.1401 gene set DNA under PCR amplification. The whole length of gene nucleotide sequence is 3090 basic groups, and coding 1029 amino acid. The recombinase features are that enzyme is quart-subunit albumen; polymer molecular weight are 391kD; mono subunit are 97.8kD; the enzyme to lactose Km value is 0.32mmol/L, Vmax is 210.70umol/(L.min); and it to onitrophenol-beta-D-galactoside Km value is close to 0; its optimum reaction temperature is 35 centigrade degree; the pH value is from 6.5-9.5; it can be stored at least two months at room temperature; oligomerization galactose yield is 55%; receptor substrate specificity of the enzyme turning glycosyl is very wild; it can used to compose multi galactoside compounds. The gained enzyme gene can be used to modify gene to gain new beta-galactoside synthetase, and improve its combined efficiency fundamentally.

The invention discloses a strain of Enterobacter cloacae subsp. dissolvens SDM and its application in the preparation of 2,3-butylene glycol. The preservation number of the Enterobacter cloacae subsp. dissolvens SDM is CGMCC No.4230. Pentose, hexose, molasses, starch raw material hydrolysate, lignocellulose raw material hydrolysate are utilized in the strain provided by the invention with high transformation rate, high product concentration and wide substrate spectrum. According to the invention, the application of the strain of Enterobacter cloacae in the preparation of 2,3-butylene glycol has advantages of simple operation method, low cost and high efficiency; the output of 2,3-butylene glycol by the fermentation cylinder fed batch fermentation can reach 125-162 g/L; the transformation rate accounts for 90-95% of the theoretical transformation rate; the maximum productivity of 2,3-butylene glycol is 4.1 g/(L<.>h). Thus, the invention has a great prospect for industrial popularization and application.

The invention discloses a novel compound microorganism fertilizer. The novel compound microorganism fertilizer is prepared from the following raw materials in parts by weight: 50 to 70 parts of an organic fertilizer, 20 to 30 parts of an inorganic fertilizer, 5 to 10 parts of a regulator and 3 to 5 parts of microorganism bacterial powder, wherein the microorganism bacterial powder is prepared from the following raw materials in parts by weight: 5 to 10 parts of enterobacter cloacae, 5 to 8 parts of serratia carollera phosphaticum, 3 to 5 parts of bacillus mucilaginosus and 2 to 3 parts of rhodospirillum fulvum. The novel compound microorganism fertilizer is prepared through the following steps: adding the enterobacter cloacae, the serratia carollera phosphaticum, the bacillus mucilaginosus and the rhodospirillum fulvum into fermentation equipment for fermenting respectively; after adsorbing fermented bacterium liquid, mixing and crushing to obtain the compound microorganism bacterial powder; mixing, crushing, granulating, drying, enveloping and packaging the organic fertilizer, the inorganic fertilizer, the regulator and the microorganism bacterial powder. The novel compound microorganism fertilizer has good economic benefits, social benefits and ecological benefits.

The invention discloses a salt-resistant ammonia-nitrogen-removing compound bacterial agent for printing and dyeing wastewater treatment. The salt-resistant ammonia-nitrogen-removing compound bacterial agent is prepared from, by mass, 5-8% of a saline-alkaline-resistant aliidiomarina haloalkalitolerans culture, 5-7% of a salt-resistant nesterenkonia halotolerans culture, 20-22% of a zobellella taiwanensis culture, 15-18% of a comamonas aquatica culture, 10-12% of a pseudomonas putida culture, 10-15% of a bacillus subtilis culture, 5-7% of an arthrobacter simplex culture and 17-18% of an enterobacter cloacae culture. The salt-resistant ammonia-nitrogen-removing compound bacterial agent for the printing and dyeing wastewater treatment contains various strains, is reasonable in compatibility,has a strong synergistic effect, can effectively remove ammonia nitrogen and industrial COD in printing and dyeing wastewater, has low input cost, and can be promoted and used on a large scale.

Bacterial compositions effective for inhibiting fungal diseases of potatoes and/or potato sprouting are produced by co-culture of two or more of Pseudomonas fluorescens (NRRL B-21133), Pseudomonas fluorescens biovar (NRRL B-21053), Pseudomonas fluorescens (NRRL B-21102) and Enterobacter cloacae (NRRL B-21050). Compositions produced by co-culture of these bacteria together in the same culture medium are significantly more effective for inhibiting fungi-induced diseases of potatoes and/or inhibiting sprouting of potatoes, than blends or mixtures of the same bacteria cultured separately.

The invention relates to the technical field of bioremediation of heavy metal mercury pollution and discloses enterobacter cloacae, a microbial inoculum containing the enterobacter cloacae, a use of the microbial inoculum and a method for passivation of mercury. The enterobacter cloacae has an accession number of CGMCC NO: 10850. The method for passivation of mercury comprises contacting the enterobacter cloacae having the accession number of CGMCC NO: 10850 and/or the microbial inoculum containing the enterobacter cloacae having the accession number of CGMCC NO: 10850 with a mercury-polluted sample so that mercury in the mercury-polluted sample is passivated. The enterobacter cloacae has high degradation ability to mercury, has a degradation rate of 85.5%, can tolerate high concentration mercury pollution, is easy to operate and can play an important role in bioremediation of mercury-polluted soil or mercury-containing wastewater.

The invention discloses enterobacter cloacae having a phosphorus solubilizing function and used for degrading imazethapyr and relates to enterobacter cloacae. The enterobacter cloacae have the functions of degrading imazethapyr and degrading indissolvable phosphorus into soluble phosphorus, thereby laying a foundation for solving the problem of soil pollution caused by residues of a herbicide imazethapyr by means of micro-biological degradation. The enterobacter cloacae T02 are preserved in CGMCC with the preserving address: #3, Yard 1, Beichen West Road, Chaoyang District, Beijing on Jule 9, 2014 and have a preservation number: CGMCC No. 9287. The enterobacter cloacae T02 not only have the function of degrading imazethapyr, but also have the function of degrading indissolvable phosphorus into soluble phosphorus. The enterobacter cloacae are used for degrading residual herbicide imazethapyr in soil.

The invention relates to degrading bacteria capable of efficiently degrading pesticide chlorothalonil, and belongs to the field of biotechnology. A classification name of the degrading bacteria is Enterobacter cloacae B9; and a culture preservation number is CGMCCNo. 5527. Chlorothalonil is an organic chlorine farm-oriented broad spectrum bactericide and is proved to have very strong effects of carcinogenicity, teratogenicity and mutagenicity. Micro-biological degradation gains more and more significance because of rich bacteria resources in the environment without secondary pollution caused by introducing other chemical reagents. Microbe BJQ2 capable of degrading the chlorothalonil can be obtained by directional culture. The Microbe BJQ2 is identified as the Enterobacter cloacae B9 by morphology, physiological and biochemical analysis and 16SrDNA sequence homology alignment. The bacteria are the degrading bacteria by using the chlorothalonil as a sole carbon source. A degrading rate is 79.2% after being cultured for 6 days in an inorganic salt culture liquid; and a degrading rate is 92.3% after being used in a soil for 7 days. The degrading bacteria can be used for degradation of the bactericide chlorothalonil, and biological purification of water bodies, soil or agricultural products which are polluted by the chlorothalonil.

The invention belongs to the field of examination of microorganisms, and relates to a method for quickly identifying bloodstream infection bacteria by a high-resolution fusion curve method. The method comprises the following steps of: performing smear gram staining on a blood culture positive culture flask to distinguish negative bacteria and positive bacteria; selectively amplifying different 16S fragments for the negative bacteria and the positive bacteria; and analyzing by the high-resolution fusion curve method. Common bloodstream infection pathogenic bacteria comprise staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, enterobacter cloacae, Serratia marcescens, Klebsiella oxytoca, acinetobacter lwoffii, enterobacter aerogenes, stenotrophomonas maltophilia and burkholderia cepacia. By the method, the common bloodstream infection pathogenic bacteria can be measured quickly, efficiently and accurately, so that strong evidences are provided and precious time is striven for subsequent treatment.

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter cloacae and belongs to the technical field of biological detection. The enterobacter cloacae specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-CATGACACCGGTGTTTCCCCAGT-3'; and the reverse primer R is 5'-CGGTCGGTGAAGCCCAGAACCACTA-3'. A detection method for detecting the enterobacter cloacae, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

The invention discloses enterobacter cloacae SRPG-70 which is screened from Sinkiang salinity soil and has the effect of remarkably relieving salt stress to promote crop growth. Authentications in the aspects of physiological and biochemical characteristics show that the enterobacter cloacae SRPG-70 is a gram negative bacterium. By utilizing the characteristics of the enterobacter cloacae SRPG-70, pot experiment proves that the germination rate in treatment of 0.8% of NaCl is improved by 22.4% relative to sterile treatment; under salinization condition, the dry weight of cotton is improved by 28.8% by the SRPG-70; the plant height is increased by 14.2%; under salinization-free condition, the dry weight of cotton is improved by 14.9% by the SRPG-70; the plant height is improved by 11.5%. Through electron microscopy observation, the SRPG-70 can be successfully colonized at the middle parts of root tips and root base parts of plant roots. The cotton inoculated with the SRPG-70 is capable of adjusting the uptake, accumulation, transportation and distribution of metal ions. The SRPG-70 can be used for replacing chemical fertilizers to provide salt stress relieving and growth promoting effects and has the advantages of being strong in adaptive capacity to environment and stable in application effect; therefore, the SRPG-70 can be used for developing microbial agents and has wide application prospect to solve the soil salinization problem.

The invention belongs to the technical field of plant growth promoting rhizobacteria and particularly relates to inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 and application thereof.The inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 is taxonomically named as Enterobacter cloacae NG-33 and preserved in the China Center for Type Culture Collection, and a preservation number of the plant growth promoting rhizobacteria strain NG-33 is CCTCC M 2016056.The plant growth promoting rhizobacteria strain NG-33 is obtained for the first time by screening and separating from soil of the Longgang National Natural Reserve in the Guangxi Zhuang Autonomous Region and is capable of realizing high-yield gluconic acid and acetic acid, dissolving hardly-soluble inorganic phosphorus to increase soluble phosphorus content in soil, relieving phosphorus deficiency of plants and improving soil conditions, and accordingly plant growth is promoted.

The invention belongs to the technical fields of molecular biological techniques and clinical tests, and particularly discloses a nosocomial infection multiple causative agent parallel detection gene chip and a preparation method and application thereof. The gene chip can be used for rapidly and sensitively detecting fourteen medical treatment infection typical causative agents such as acinetobacter baumannii, enterobacter cloacae, enterococcus faecalis and the like by using one reaction, and the detection data of coinfection of yeast and bacterium which cannot be provided by a traditional amplification method is offered. Furthermore, the gene chip also comprises a methicillin resistance target spot, thus realizing further detection of staphy lococcus infection, therefore, a clinician can realize which causative agent a patient is infected in one or two hours, and realize whether the patient has drug resistance, so as to decide whether the patient needs antibiotics or which antibiotics is needed, so that the gene chip has a greater clinical guiding significance.

The invention discloses a bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil. According to the bagasse immobilized bacterium, Enterobacter cloacae is immobilized by adopting bagasse, and when the Enterobacter cloacae is inoculated to a bagasse vector, a bacterial suspension is prepared by culturing in advance, and then the Enterobacter cloacae is immobilized onto the bagasse vector treated by an inorganic salt culture solution to establish an efficient and stable agricultural waste immobilized bacterium system. The bagasse immobilized bacterium is low in cost, and when the soil is remediated, simplicity and environment-friendliness are achieved, and secondary pollution is avoided; in addition, the bioavailability of sulfonamides can be improved, and the microbiological degradation efficiency of the sulfonamides is improved.