113 results about "Rhizobacteria" patented technology

A method for promoting the health of a plant comprises administering malic acid to the plant or the soil in an amount effective to recruit plant growth promoting rhizobacteria (PGPR) to the plant. Administration of malic acid promotes biofilm formation of PGPR on the plant's roots, thereby restricting entry of a foliar pathogen through stomatal pores present in the leaves. Another method for promoting the health of a plant comprises administering acetoin to the plant or the soil in an amount effective to increase pathogen resistance in aerial parts of the plant.

Lipo-chito oligosaccharides (LCOs) are produced by culturing rhizobacteria cells in or on a culture medium comprising at least one of: jasmonic acid or a derivative thereof; linoleic acid or a derivative thereof; or linolenic acid or a derivative thereof. Preferably, the rhizobacteria cells are Bradyrhizobium japonicum cells having the identifying characteristics of B. japonicum strain USDA 3. Preferably, the derivative of jasmonic acid is an ester thereof, preferably methyl jasmonate. Also provided are methods for improving LCO production at low temperatures, particularly temperatures below 25° C.

According to the present invention new isolates of bacterial strains have been shown to possess unique properties. These bacterial strains are plant growth-promoting rhizobacterium (PGPR), posses an enhanced competitive advantage at colonizing leguminous plants, and enhance the overall performance of leguminous plant growth. Further still, the present invention discloses a novel method for screening and selecting bacterial strains having the aforementioned beneficial characteristics.

A method of plant cultivation which comprises inoculating plants in a plant growing system comprising a container and an insert therefore, the microbial inoculant containing at least one species from the following group of microorganisms comprising arbuscular mycorrhizae associated bacteria, plant growth promoting rhizobacteria, yeast microorganisms and substrate conditioning bacteria.

According to the present invention a new isolate of a Pseudomonas spp, DSM 21663, has been shown to possess unique properties. This Pseudomonas is a plant growth-promoting rhizobacterium (PGPR). Among its modes of action involved in plant growth-promotion are anti-biotic production (2,4-diacetylphloroglucinol, (DAPG); pyrrolnitrin, PRN and others), indole-3-acetic acid (IAA) production and phosphate solubilization, and production of unique volatiles. The strain is fluorescent, oxidase-positive, and has the ability to suppress soil-borne root and foliar pathogens of both fungal and bacterial origin.

The invention provides a soil remediation method and application thereof. The method comprises the steps: planting remediation plants in soil, and applying a biological remediation agent into the soil, wherein the remediation plants are one or a combination of annual cross-bred forage grass sorghum and annual cross-bred forage grass foxtail millet which can be cut for many times; the biological remediation agent at least comprises a fermentation raw bacteria liquid which contains plant growth promoting rhizobacteria (Pseudomonas cedrina UW3 and Pseudomnonas Putida UW4); the preparation of the fermentation raw bacteria liquid is described in the invention patent-ZL2008 1 0181327.8z; the soil is soil damaged by heavy metals which include As, Cd, Co, Cr, Cu, Hg, Ni, Pb, Zn, radioactive U235 and the like.

The invention belongs to the field of agricultural microbiology, and relates to plant growth-promoting rhizobacteria and an application thereof. The plant growth-promoting rhizobacteria (Pseudomonas fluorescens Y1) was collected in the General Microbiological Center of China Microbiological Culture Collection Management Committee on January 11th, 2013, and is named Pseudomonas fluorescens in a classified manner, and the collection number is CGMCC (China General Microbiological Culture Collection Center) NO. 7130. The strain utilizes insoluble phosphate as a phosphorus source for strong growth ability, can improve the utilization ratio of the fertilizer, promotes the development of plant roots and uptake of a fertilizer and increases the phosphorus content of the plant. The plant growth-promoting rhizobacteria disclosed by the invention has a good growth promoting effect for peanuts, and the efficient phosphate solubilisation of the strain promotes the growth and the development of the peanuts and the utilization ratio of a phosphorus fertilizer.

The invention relates to a comprehensive treatment agent for water bodies and bottom mud of river channels and a method for preparing the comprehensive treatment agent, and belongs to the field of water environment protection. The comprehensive treatment agent is of a multilayer spherical structure and sequentially comprises a submerged plant seed layer, a composite biological preparation layer and an oxygen release layer from the inside to the outside. The submerged plant seed layer comprises submerged plant seeds, activated carbon and PGRR (plant growth promoting rhizobacteria) preparations;the composite biological preparation layer comprises attapulgite, activated carbon, bentonite and microbial agents; the oxygen release layer comprises calcium peroxide, kaolin and quartz sand. The comprehensive treatment agent and the method have the advantages that the comprehensive treatment agent prepared by the aid of the method can be directly spread to the river channels and gradually sinksat water bottoms, oxygen can be slowly released, nitrogen, phosphorus and heavy metal in the water bodies can be treated by mineral and microbial preparations in fillers, submerged plants ultimatelygrow and breed, accordingly, the river channels can be ecologically restored, and the ornamental value of landscape of the river channels can be improved.

The invention discloses an alfalfa growth-promoting rhizobacteria MJM-11 and application thereof. An Enterobacter ludwigii strain MJM-11 has a preservation number of CGMCC No.6295. The Enterobacter ludwigii strain MJM-11 can be applied in at least one of the following four ways: 1, preparation of an ACC (1-aminocyclo propane-1-carboxylate) ammonialyase; 2, preparation of indoleacetic acid; 3, preparation of siderophores; and 4, promotion of the growth of plants under the saline-alkali stress. Experiments prove that the alfalfa growth-promoting rhizobacteria MJM-11 which is obtained through separation can be used for synthesizing the ACC ammonialyase and the IAA (Indole Acetic Acid) siderophores and is capable of effectively promoting the alimentation of plants in a saline-alkali stress environment, regulating the growth of plants and enhancing the stress resistance capacity of plants in adverse conditions.

The invention discloses ectomycorrhizal helper bacteria bacillus pumilus HR10. The classified nomenclature of the ectomycorrhizal helper bacteria bacillus pumilus HR10 is bacillus pumilus, the bacillus pumilus is preserved in CCTCC, the collection number of the bacillus pumilus is CCTCC No: M 2010143 and the preservation date is June 12th, 2010. The bacillus pumilus HR10 is plant growth-promoting rhizobacteria, has direct promoting effect on black tree growth and can promote the growth of rhizipogen luteous of ectomycorrhizal fungi; double inoculation of the bacillus pumilus HR10 and the rhizipogen luteous has obvious growth-promoting effect on pinus thunbergii parl; the bacillus pumilus HR10 has inhibiting effect on pathogenic rhizoctonia of pine nursery stock damping-off disease; and greenhouse preventing and controlling experiments show that the bacillus pumilus HR10 strains can effectively prevent and control the pine nursery stock damping-off disease.

A method using pseudomanas vesicularis to promote plant to repair the soil polluted by Ni features that said pseudomanas vesicularis, which can secrete Fe arrier and decrease the content of ethene in plant, is inoculated to the root of plant growing in said polluted soil for increasing the effect of plant to absorb the Ni, so repairing the soil.

The invention discloses growth-promoting rhizobacteria and fungicide thereof, as well as a preparation method and application of the fungicide, namely Klebsiellapneumoniae and the fungicide taking the Klebsiellapneumoniae used as an active ingredient, the preparation method of the fungicide and application of the fungicide in phosphorus dissolving and nitrogen fixation. The invention has the advantages that the nitrogen fixing and phosphorus dissolving bacterium integrated with multiple growth-promoting capabilities, such as phosphorous dissolving, nitrogen fixing and auxin producing, into a whole is provided to prepare the nitrogen fixing and phosphorous dissolving fungicide, the fungicide has the characteristics of complete growth promotion performance, high fertilizer efficiency and low cost, and is suitable for the regions lacking nitrogen and phosphorous and having hungry soil; and the preparation process is simple and convenient, high fertilizer efficacy of nitrogen fixing and phosphorous dissolving is obtained, and the invention has excellent development prospect.

Multiple-effect plant growth-promoting bacteria and an isolation and screening method are disclosed. The invention belongs to the field of agricultural microbiology and specifically relates to multi-effect plant growth-promoting rhizobacteria with phosphorus-solubilizing, NH3-producing, plant growth hormone-producing and siderophore-producing effects. The preservation number of the multi-effect plant growth-promoting bacteria is CGMCC No. 14664. The multiple-effect plant growth-promoting bacteria are applied to fertilizers and microbial inoculants. The isolation and screening method of the invention includes sampling, cultivation of strains, identification and selection of strains, preliminary selection of strains, re-selection of strains, signing of strain performance and identification of multi-effect strains.

The invention relates to maize growth-promoting rhizobacteria YM4 and an application thereof. The maize growth-promoting rhizobacteria are the maize growth-promoting rhizobacteria YM4, and are classified to be named as Bacillus pumilus; the maize growth-promoting rhizobacteria YM4 are collected in the General Microbiological Center of China Committee for Culture Collection of Microorganisms on October 31, 2014, and a collection number of the maize growth-promoting rhizobacteria YM4 is CGMCC No.9898; by adopting the maize growth-promoting rhizobacteria YM4, the problem of converting difficultly-soluble potassium-containing silicate into soluble potassium salt can be effectively solved, organic phosphorus can be degraded, and the utilization rate of fertilizers can be improved; and the maize growth-promoting rhizobacteria YM4 can be effectively applied to the planting of maize, can be used for promoting the growth of maize and improving the yield, and is a great innovation of microorganism and maize planting.

A biologically pure phosphate solubilizing plant growth promoting rhizobacteria (PGPR) XSB375 was isolated from canola rhizosphere and identified as Bacillus firmus is provided herein. The PGPR is capable to solubilize phosphate that enhance plant available phosphorous. The PGPR XSB375 enhances seed germination, early emergence, plant vigor, root and shoot growth, and higher crop yield. This increased crop growth, development and yield is attributed from not only the greater phosphate solubilization making more available phosphorus to plant but also making other essential plant nutrients more available to the plant and the like. The application of this phosphate solubilizing PGPR can be done as liquid suspension or solid materials using onto soil, potting mix, seeds, seed pieces, seedlings, foliage, carrier materials, roots and planting soil.

The invention discloses a production process for relay fermentation of novel plant growth promoting rhizobacteria type bio-organic fertilizer. According to the technical scheme, the production process comprises the steps that a nutritional package I of functional growth promotion microorganisms is made, functional microorganisms are inoculated and a nutritional package II is added in the later period of compost fermentation, breeding of the inoculated functional microorganisms is promoted by adding the nutritional package II, and the novel bio-organic fertilizer is made through the plant growth promoting rhizobacteria in a relay fermentation mode. The production process for relay fermentation of the novel plant growth promoting rhizobacteria type bio-organic fertilizer has the advantages that the environmental friendliness and resource conservation can be achieved to the greatest extent in the way of increasing soil fertility through activities of microorganisms, waste serves as a nutrient resource replacing chemical fertilizer, farmland and a water environment are purified, the production cost of growth promotion microorganism organic fertilizer is reduced, and agricultural extension is facilitated. The production process has comprehensive benefits such as the economic benefit, the ecological benefit, the environmental benefit and the social benefit.

The invention provides ardisia japonica phyllobacterium RC6b and application of the ardisia japonica phyllobacterium RC6b in soil remediation. The strain is stored in a depository authority specified by State Intellectual Property Office with a number of CGMCC No. 6621 on September 25, 2012; and the depository authority is a common microbe center of China Committee for Culture Collection of Microorganisms. The plant growth promoting rhizobacteria is high in heavy metal resistance and absorbing performance, can obviously promote t growth of a plant, reduce toxicity of heavy metal to plant, and improve phyto-availability of the heavy metal in soil, so that the ardisia japonica phyllobacterium RC6b can be directly adopted for improving plant repair efficiency; and the ardisia japonica phyllobacterium RC6b has a good application prospect in bioremediation of the soil polluted by the heavy metal.

Biological nitrogen fixation is a very important process to plant growth and health. Biological nitrogen fixation is performed naturally by a number of different microorganisms including plant growth-promoting rhizobacteria, mycorrhizal fungi, and rhizobia. The present invention discloses novel compositions comprising water soluble polymers, rhizobium and plant-growth promoting rhizobacteria. Uses of the novel composition include treatment of seeds prior to planting and use in soil surrounding roots to improve plant growth and health.

The invention relates to peanut growth promoting rhizobacteria HS2 and an application thereof. The peanut growth promoting rhizobacteria is peanut growth promoting rhizobacteria HS2 which is classified and named bacillus subtilis and collected in the China General Microbiological Culture Collection Center on October 31, 2014, with a collection number CGMCC No.9888; the insoluble potassium-containing silicate can be effectively converted into soluble potassium salt, organophosphorus is dissolved, and the utilization rate of fertilizer is improved; and the peanut growth promoting rhizobacteria HS2 is effectively applied to peanut planting to promote the peanut growth and increase the yield, and the application is a great innovation in microorganism and peanut planting.

The invention belongs to the technical field of plant growth promoting rhizobacteria and particularly relates to inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 and application thereof.The inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 is taxonomically named as Enterobacter cloacae NG-33 and preserved in the China Center for Type Culture Collection, and a preservation number of the plant growth promoting rhizobacteria strain NG-33 is CCTCC M 2016056.The plant growth promoting rhizobacteria strain NG-33 is obtained for the first time by screening and separating from soil of the Longgang National Natural Reserve in the Guangxi Zhuang Autonomous Region and is capable of realizing high-yield gluconic acid and acetic acid, dissolving hardly-soluble inorganic phosphorus to increase soluble phosphorus content in soil, relieving phosphorus deficiency of plants and improving soil conditions, and accordingly plant growth is promoted.

The invention discloses a method for producing full water-soluble solid bio-organic fertilizer. The method comprises the following steps: generating waste water rich in varied nutrient substances based on fermentation of yeasts, and concentrating the waste water into semiliquid; flash drying the semiliquid to dried powder; supplementing nitrogen, phosphor and potassium nutrition to the dried powder and adding plant rhizosphere growth-promoting rhizobacteria. According to the method, fermented waste liquid is deeply processed, the plant rhizosphere growth-promoting rhizobacteria are special functional bacteria which can preferably utilize amino acids and vitamins to quickly propagate and form dominant bacteria within the soil range that fertilizers can reach, and the metabolites of the dominant bacteria can promote the growth of plants as well as restrain or interfere with the propagation of other bacteria, thereby achieving the effect of further improving the fertilizer efficacy and restraining the propagation of pathogenicbacteria.

The invention discloses a method for screening multifunctional bacterial for degrading organophosphorus pesticides. The method comprises the following steps of: (1) activating or screening plant growth-promoting rhizobacteria; (2) domesticating strains; (3) degrading the activity of the organophosphorus pesticides by using the strains; (4) optimizing the influence of environmental factors on the capacity of degrading the organophosphorus pesticides of the strains, and optimizing the condition; and (5) researching the degrading effect on the organophosphorus pesticides in soil of the strains through basin and earthen bowl tests to analyze the biological remediation potential of the strains to the soil contaminated by the organophosphorus pesticides. The bacterial obtained through the method can keep the stabilities of disease-preventing and growth-promoting functions and capacity of degrading the organophosphorus pesticides for a long time; due to the diversity of the functions, the bacterial can survive and propagate easily after being applied into soil, so that the functions of the bacterial can be given into full play; and besides, the method is simple to operate, low in cost and time-saving and labor-saving. Therefore, the method can have an extensive application prospect in the fields of screening the multifunctional bacteria, performing microbial remediation on the soil contaminated by the organophosphorus pesticides, and the like.

The invention provides a method for improving cadmium resistance of tomatoes. The method includes the steps: 1) transplanting tomato seedlings into cadmium contaminated soil and inoculating AMF (arbuscular mycorrhizal fungi) bacteria; 2) inoculating plant growth promoting rhizobacteria to a plant root system; 3) culturing the tomatoes to be mature and generate fruits. By co-inoculation of the plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi, growth of the tomatoes is promoted under cadmium stress, the content of heavy metals in plants can be reduced without affecting soilproperties, directions are provided for research of combined effects of the plant growth promoting rhizobacteria and the AMF on plant growth and metal resistance, and routes are provided for exploringthe action mechanism of the plant growth promoting rhizobacteria and the AMF.

The invention relates to the field of plant growth-promoting rhizobacteria (PGPR), in particular to Bacillus aryabhattai for promoting corn root system development and application of the Bacillus aryabhattai. The strain is Bacillus aryabhattai LAD, and is preserved in China General Microbiological Culture Collection Center, wherein the preservation site is No.3, Yard 1, Beichen West Road, ChaoyangDistrict, Beijing, the preservation number is CGMCC NO: 18653, and the preservation time is October 10, 2019. The invention relates to an application of the Bacillus aryabhattai in promoting corn root development. The Bacillus aryabhattai LAD can stably exist in soil, has the characteristics of nitrogen fixation, sugar production, starch decomposition, plant hormone production and the like, and promotes the growth of corn seedlings and the development of root systems. Moreover, the Bacillus aryabhattai has relatively strong stress resistance, so that the Bacillus aryabhattai can be applied tocrop production.

The invention discloses a growth-promoting rhizobacteria SXH-2 with a categorical name of klebsiella oxytoca SXH-2. The strain is collected in China General Microbiological Culture Collection Center with a collection number of CGMCC No. 6297. The strain can synthesize indole acetic acid and siderophore, and has a phosphate-solubilizing effect. Therefore, the strain assists in providing indole acetic acid and iron element for plants, and assists in promoting phosphorus absorption. The strain assists in promoting plant growth. The strain has substantial promoting effect upon plant height, fresh weight and dry weight of leeks.

The invention discloses a seedling growing matrix capable of improving the survival rate of afforestation of non-woven fabric container seedlings. The seedling growing matrix is composed of a matrix host material and a water-retaining agent. The matrix host material is composed of the following constituents, by weight, 40-50 parts of turf, 50-60 parts of fermentation carrier matrices and 10-20 parts of vermiculite. The addition quantity of the water-retaining agent is 3-4%. that of the matrix host material. The seedling growing matrix is suitable for cultivating container seedlings and applicable to improving the survival rate of afforestation of the non-woven fabric container seedlings in arid and barren mountains, and furthermore, the matrix and plant growth promoting rhizobacteria are used in a combined mode, so that the survival rate of afforestation is higher. The invention further discloses a method for improving the survival rate of afforestation of the non-woven fabric container seedlings. The method includes the steps that the seedling growing matrix is used for cultivating the non-woven fabric container seedlings first, before the non-woven fabric container seedlings are transplanted and fixedly planted, the plant growth promoting rhizobacteria are inoculated, and then transplanting and fixed planting are carried out.

The invention relates to plant-microorganism combination remediation technologies of DDT-contaminated soil, in particular to a method and application for remedying DDT-contaminated soil through a plant-microorganism combination. According to the method and application for remedying the DDT-contaminated soil through the plant-microorganism combination, the DDT-contaminated soil is remedied in the manner that ryegrass is planted in the DDT-contaminated soil and DDT degrading bacteria and plant growth promoting rhizobacteria are exerted on the DDT-contaminated soil, then overground ryegrass is harvested, and DDT in the soil is removed. The method and application for remedying the DDT-contaminated soil through the plant-microorganism combination comprise the following steps that in a DDT-contaminated farmland greenhouse, before the ryegrass is planted, a plough layer of the soil is turned over and flattened manually, and the DDT degrading bacteria are added; the ryegrass is sown, weeding is conducted at regular intervals, and after the ryegrass grows for one month, the plant growth promoting rhizobacteria are added; the overground part and the underground part of the ryegrass are harvested; and the DDT contents of the overground part and the underground part of the ryegrass are measured, and the DDT removal amount of the soil is measured. By the adoption of the method and application for remedying the DDT-contaminated soil through the plant-microorganism combination, the DDT in the soil can be effectively removed, the degradation rate of the DDT is high, the DDT content of the overground part of the ryegrass is low, and the risk that the DDT enters a food cycle along with the plants is lowered; and the method is simple in operation, safe, high in efficiency, low in cost and high in economic and social benefit.

The invention relates to a method for promoting plants to restore soil hardening through utilizing Acetobacter xylinum, and belongs to the technical field of the agriculture. The method is characterized in that the method comprises the following steps: carrying out expanding culture of Acetobacter xylinum strains to prepare a bacterial liquid, wherein the concentration of the bacterial liquid, represented by OD600, is not less than 0.8 (the OD600 is generally between 0.8 and 1.5); controlling the pH value of soil at 5.5-7; and processing the soil restoration plants by the bacterial liquid. The method has the following beneficial effects: the Acetobacter xylinum selected in the invention can promote the growth of the plants and improve the biomass of the plants through the symbiotic relation between rhizosphere bacteria and the plants, so the rich water absorption of the plants and the hardened soil restoration effect are improved, and potential economic effects are apparent; and the used strains improve present green in-situ restoration effects, and gained plant overground parts can generate economic effects and also can improve the soil degeneration and the productivity decrease caused by the soil pollution of fertilizers and the like.