The invention discloses an efficient
tissue culture and rapid propagation technology for seedlings of
bletilla striata. According to the technology,
bletilla striata seeds are sown on a seed
germination culture medium for sterile
germination, the obtained seedlings are inoculated to a multiplication and subculture medium one by one for multiplication and subculture, clustering seedlings are obtained and inoculated to a rooting culture medium for rooting culture, the obtained
tissue culture seedlings are planted on a prepared
seedling hardening substrate, and the seedlings are transplanted to a large filed for plantation after
seedling hardening, wherein the seed
germination culture medium comprises MS (
magnesium sulfate), 30g / L of
sugar, 8g / L of
agar and 10g / L-12g / L of potatoes; the multiplication and subculture medium comprises MS, 1.0 mg / L of KT (
kinetin), 0.2 mg / L of NAA (
naphthalene acetic acid), 30g / L of
sugar and 9g / L of
agar; the rooting culture medium comprises MS, 0.2 mg / L of NAA, 1.0mg / L of IAA (
indole acetic acid), 30g / L of
sugar, 9g / L of
agar and 25-30 g / L of potatoes; the
seedling hardening substrate comprises medium moor
peat soil,
perlite and wood dust in the volume ratio being 5:2:3. The technology is mature, a
system is perfect, the problems of low natural germination rate of
bletilla striata and difficulty in obtaining of seedlings can be effectively solved, and the technology has better popularization and application value and broad market prospect.