Method for rapidly breeding potted carnation through tissue culture
A tissue culture, carnation technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as provenance degradation, inter-variety hybridization, and flower color confusion, and achieve the effect of high work efficiency and simple procedures.
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Embodiment 1
[0022] [embodiment 1] a kind of method of tissue culture rapid breeding potted Carnation, it comprises the following steps:
[0023] S1: Explant explants are cut and sterilized, which includes the following sub-steps:
[0024] (1) Select the side buds of potted Carnation without disease, wash them in running water for 1 hour, and then absorb the water;
[0025] (2) Put it into the ultra-clean workbench and disinfect it with 70% alcohol for 35 seconds;
[0026] (3) Rinse 4 times with sterile water, put in 0.1% mercuric chloride to sterilize for 8 minutes;
[0027] (4) Rinse 4 times with sterile water and place on sterile paper to dry.
[0028] S2: Primary culture: cut the sterilized lateral buds into 1.5cm-diameter stems with buds, and inoculate them in the primary medium for culture, wherein the primary medium is MS+6-BA1.5mg / L+NAA 0.2mg / L + sucrose 30g / L + agar 8g / L, the culture conditions are: light intensity 2200LX, light time 15h, temperature 23°C, incub...
Embodiment 2
[0032] [embodiment 2] a kind of method of tissue culture rapid breeding potted carnation, it may further comprise the steps:
[0033] S1: Explant explants are cut and sterilized, which includes the following sub-steps:
[0034] (1) Select the side buds of potted Carnation without disease, wash them in running water for 2 hours, and then absorb the water;
[0035] (2) Put it into the ultra-clean workbench and disinfect it with 75% alcohol for 28 seconds;
[0036] (3) Rinse 5 times with sterile water, put in 0.2% mercuric chloride to sterilize for 5 minutes;
[0037] (4) Rinse 6 times with sterile water and dry on sterile paper.
[0038] S2: Primary culture: Cut the sterilized lateral buds into 2.5cm-diameter stems with buds and inoculate them in the primary medium for culture, wherein the primary medium is MS+6-BA1.5mg / L+NAA 0.2mg / L + sucrose 30g / L + agar 8g / L, the culture conditions are: light intensity 1800LX, light time 20h, temperature 25°C, incubation ti...
Embodiment 3
[0042] [embodiment 3] a kind of method of tissue culture rapid breeding potted Carnation, it comprises the following steps:
[0043] S1: Explant explants are cut and sterilized, which includes the following sub-steps:
[0044] (1) Select the side buds of potted Carnation without disease, wash them in running water for 1.5 hours, and then absorb the water;
[0045] (2) Put it into the ultra-clean workbench and disinfect it with 72.5% alcohol for 30 seconds;
[0046] (3) Rinse 5 times with sterile water, put in 0.15% mercuric chloride to sterilize for 6 minutes;
[0047] (4) Rinse 5 times with sterile water and dry on sterile paper.
[0048]S2: Primary culture: Cut the sterilized lateral buds into 2cm-diameter stems with buds, and inoculate them in the primary medium for culture, wherein the primary medium is MS+6-BA1.5mg / L+NAA 0.2mg / L L + sucrose 30g / L + agar 8g / L, the culture conditions are: light intensity 2000LX, light time 18h, temperature 24°C, incubation...
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