100 results about "Dianthus" patented technology

The invention discloses an efficient virus-induced phytoene desaturase gene silence system for Chinese pink. A specific primer is designed according to a coding gene sequence of PDS (phytoene desaturase), and about 500bp cDNA (complementary deoxyribonucleic acid) fragments are amplified in the Chinese pink through RT-PCR (reverse transcription-polymerase chain reaction). Obtained PDS cDNA fragments are connected to TRV2, and acetosyringone concentration, seedling age and preculture temperature after infection as well as light conditions for plants after preculture are integrated in an assorted manner by optimizing an agrobacterium-mediated virus-induced gene silence system according to photobleaching frequency, bleached leaf area and bleached conditions of the plants, so that the rapid efficient virus-induced silence system is established, virus-induced gene silence character can be obtained, and further, the problem that Chinese pink gene functions are verified effectively is solved.

The present invention relates to a Chinese medicine preparation for curing heat strangury. The prescription of said Chinese medicine is formed from 10 Chinese medicinal materials of lonicera flower, bearded scutellaria, dianthus, knotgrass, pyrrosis leaf and others.

The invention discloses a Paris polyphylla and rhizome polygonati efficient interplanting method. A three-layer circulating interplanting method is used. The method comprises: in first layer soil, interplanting Chinese torreya trees on the upper layer, in a three-dimensional growing environment whose ground surface plant is pipewort, interplanting two rows of Paris polyphylla between each two rows of Chinese torreya trees, interplanting a row of pipewort between two rows of Paris polyphylla, in second layer soil, interplanting pink on the upper layer, interplanting two rows of Paris polyphylla between each two rows of pink, interplanting a row of rhizome polygonati between two rows of Paris polyphylla, in third layer soil, interplanting dogwood on the upper layer, interplanting two rows of Paris polyphylla between each two rows of dogwood, interplanting a row of milk vetch between two rows of Paris polyphylla, planting area of each layer being 1-2 mu, and inter-layer height vertical throw being 1-2 m. The interplanting method uses a same land to produce a plurality of kinds of medicinal materials at the same time, and cropping index is improved. The method saves labor cost, reduces production cost, improves yield, reduces pest and disease damages, and economic benefits are improved by times.

The invention relates to a sweet broomwort herbal tea and a making method of the sweet broomwort herbal tea. The sweet broomwort herbal tea is prepared from the raw materials such as green tea, tea stems, dianthus, stevia rebaudiana, momordica grosvenori flowers, selfheals and roxburgh roses. The making method comprises the following steps: (1) drying and grinding the green tea and the tea stems; (2) drying and smashing the dianthus and the stevia rebaudiana; (3) selecting the roxburgh rose and drying and grinding the roxburgh rose; (4) drying and grinding the momordica grosvenori flowers and the selfheals; and (5) packaging the product after the raw materials are mixed uniformly. The sweet broomwort herbal tea made by the making method disclosed by the invention has the health-protection effects of relieving pharyngitis, quenching the thirst, promoting digestion, reducing three-highs (high cholesterol, high blood pressure, high blood sugar), improving constipation and the like and in particularly has good health-protection effects on the pharyngitis, the diabetes and the constipation.

The invention discloses a method for preparing the filial generation of dianthus caryophyllus and dianthus chinensis, which comprises: 1, obtaining the protoplast of the dianthus caryophyllus and the dianthus chinensis respectively; 2, performing protoplast asymmetric fusion of the protoplast of the dianthus caryophyllus and the protoplast of the dianthus chinensis to obtain fused protoplast; and 3, culturing the fused protoplast to grow the protoplast into a hybrid plant. The invention provides the first method suitable for culturing the filial generation of the protoplast of the dianthus caryophyllus and the dianthus chinensis, overcomes the drawback that it is difficult to obtain the filial generation of the dianthus caryophyllus and the dianthus chinensis in the prior art and, by optimizing cross conditions, cultures novel filial generation which has good plant shape, high ornamental performance and high quantity of mu plant rate for the first time.

The invention relates to a tissue culture method of dianthus deltoids. The tissue culture method comprises the steps of taking stem apex of dianthus deltoids twigs as an explants; subjecting the stem apex to sterilization; and culturing by inoculating the stem apex to a primary induction medium composed of MS, 0.01 mg / L 6-BA and 0.05 mg / L NAA, a subculture medium composed of MS, 0.01 mg / L 6-BA and 0.05 mg / L NAA and a rooting medium composed of 1 / 2 MS and 0.05 mg / L IBA successively under culture conditions that a culture temperature is 25+ / -1 DEG C; illumination intensity is 2,500 Lx and light is supplemented for 4 hours / day at night except natural light in the daytime. The culture method can obtain large-scale indoor bred dianthus deltoids seedlings in a short time, has high production rate and has potential ecologic benefits and social benefits.

A method of transforming a carnation (Dianthus L.) plant genome with a DNA molecule. The method comprises (a) preparing stem explants from carnation cuttings; (b) wounding the explants by microprojectile bombardment; (c) cocultivating the wounded explants with Agrobacterium comprising the DNA molecule under defined conditions of exposure to dark followed by light; (d) excising shoots from the cultivated wounded explants and removing the leaves from the shoots; and (e) culturing the leaves to obtain transgenic shoots transformed with the DNA molecule. Also disclosed are a rolC-transgenic carnation with improved agronomic traits and enhancement of flower fragrance by antisense suppression of the flavonoid gene fht.

The invention provides a Chinese medicinal composition for treating pig body surface parasites and a preparation method thereof, and relates to the technical field of preparation of a pig veterinary medicine. The Chinese medicinal composition is prepared from the main raw materials: cleistocalyx operculatus bark, radix ampelopsis, yellow lycoris bulb, tobacco, Incised Corydalis Herb, wintercherry, delphinium, maritime dock, golden larch bark, Chinese mugwort leaf, sessile stemona root, mint, lemna minor, philippine violet herb, eucalyptus grandis leaves, alderleaf sida leaf, roegneria thoroldiana, senecio dianthus franch and liquorice root. By scientific compatibility and a synergistic effect of various raw materials, the Chinese medicinal composition has effects of removing toxicity and killing parasites, dispelling wind and arresting itching, inhibiting bacteria and promoting granulation and the like, can effectively remove and treat parasites of louse, mite, tick, flies, mosquitoes and the like on body surfaces of pigs and symptoms of pruritus, bleeding, abscess, dermatitis and the like, which are caused by the parasites, meanwhile, can inhibit microorganism and mould breeding on the body surfaces of the pigs, promotes regeneration of damaged skin, is safe, has no irritation and is valuable to popularize and use.

The invention belongs to the field of ornamental plant transgenic technology, particularly relates to a method for culturing transgenic dianthus and more particularly discloses a method for culturing transgenic dianthus by taking agrobacterium-mediated embryogenic callus of the dianthus as a material. The method comprises the following steps of embryogenic callus induction, genetic transformation, screening and regeneration, GUS (glucuronidase) coloration detection and PCR (polymerase chain reaction) detection. The method is characterized in that the GUS report gene and the ACO target gene are introduced into the receptor which is the embryogenic callus of the ianthus through agrobacterium mediation, different factors affecting the embryogenic callus transformation are screened, kanamycin or hygromycin is used as a screening agent to screen the transformed embryogenic callus, and the screening is carried out through GUS coloration, PCR detection and other auxiliary methods to obtain the transgenic dianthus. The invention provides a novel technology platform for the innovation of the germplasm resources for the genetic transformation of the dianthus.

The invention relates to culture mediums for tissue culturing of a maiden pink. The optimal culture mediums for different stages in the tissue culturing of the maiden pink are screened; and the matching proportions of various hormones in the culture mediums are determined, so that the culture mediums prepared according to a formula provided by the invention can meet the nutritional requirement and the growing development requirement of the maiden pink in all stages. The culture media respectively comprise: an initial induction culture medium formed by MS, 0.01mg / L of 6-BA and 0.05mg / L of NAA (Naphthalene Acetic Acid), a mulitiplication culture medium formed by the MS, 0.01mg / L of 6-BA and 0.05mg / L of NAA, and a rooting culture medium formed by 1 / 2 of MS and 0.05mg / L of IBA (Indole Butyric Acid). The culture mediums are particularly suitable for the tissue culturing of the maiden pink.

A hybrid seed production method of Dianthus chinensis, in which the female parent adopts a combination of tissue culture and cuttings, and the male parent seedlings are directly sown, and after steps such as planting, field management, pollination, etc., the seeds are finally harvested. The invention has the advantages of high seed production yield, good seed quality, less virus infection, high production efficiency and the like.

The invention discloses a tissue culture and rapid propagation method for dianthus caryophyllus L. The dianthus caryophyllus L belongs to caryophyllaceae dianthus herbaceous perennial flowers, has the advantages of high productbility, good ornamental value, easiness in storage and transportation, and the like, and is one of main culture varieties of commercial cut-flowers cultured most widely in the present world. A large number of high-quality seedlings can be obtained by plant in-vitro culture, the propagation coefficient is greatly increased, viruses carried in a plant body are reduced, the stress resistance of the whole plant is improved, the limitations of environmental conditions of seasons are avoided, and the defects of conventional cutting propagation are made up through the breeding mode. A lateral bud of dianthus caryophyllus L is taken as an explant, and a dianthus caryophyllus L in-vitro replanting plant is successfully obtained by adventive bud induction culture, multiplication culture, rooting culture, acclimatization and transplantation, and the like, a dianthus caryophyllus L tissue culture and rapid propagation system is established, and the large-scale production of dianthus caryophyllus L tissue culture seedlings is promoted.

The invention provides a method for promoting secondary blooming of dianthus chinensis plants. The method comprises the following steps that after the dianthus chinensis plants bloom for the first time and are pruned and stumped at the positions away from the ground for designated height in a designated time period; residual branches are cleared, and a designated depth of water is watered; the roots of the dianthus chinensis plants grow out lateral buds again in a first designated maintenance time duration; lateral buds develop mature after a second designated maintenance time duration; lateral buds secondarily bloom after a third designated maintenance time duration. By adopting the method for promoting secondary blooming of the dianthus chinensis plants, it can be ensured that secondary blooming of the dianthus chinensis plants can be promoted without pinching processing after flower withering, and the ornamental value of the plants is embodied again.

The invention relates to an irrigant for diminishing the inflammation of the wound, wherein the scientific combination of Paris polyphylla, Bletilla striata, Senecio scandens, Senecio dianthus Franch and borneol is adopted; pure Chinese medicinal herbs are used; the raw materials are accessible and convenient to use; and the irrigant has the functions of diminishing inflammation and promoting granulation, curing the trauma in vitro, infection of wounds, scalds, burns, deep-rooted boils and ulcers, washing the sutured wounds, preventing and curing the infection of wounds and promoting the healing of wounds.

The invention discloses a method for feeding frankliniella occidentalis indoors. The method comprises the following steps: inserting dianthus carryophyllus plants into a container filled with water; inoculating frankliniella occidentalis adults onto the plants; putting into a breeding cage; after oviposition, taking stems of the dianthus carryophyllus plants as explants; washing with running water; disinfecting with 75% ethyl alcohol and 0.1% mercuric chloride solution; washing with sterile water; then inoculating the explants onto an inducing medium; inducing multiple shoots in a tissue culture chamber under the culture conditions that the temperature is 25+ / -2 DEG C and the photoperiod is 16L:8D, wherein emerged adults are clean tested insects; transferring the tested insects onto dianthus carryophyllus tissue culture bottle seedlings; paving a layer of sterilized sand on the surface of culture medium in a tissue culture bottle; removing the adults 12 hours after the oviposition; putting back the bottle seedlings with eggs into the tissue culture chamber; and culturing until adults are emerged. The method has the advantages that plant tissue culture seedlings are adopted for feeding frankliniella occidentalis larvae and adults, the feed does not need to be replaced frequently, feeding steps are simplified, the feed is sufficient, the feeding efficiency is improved, and the survival rate is high.

The invention relates to a method for preparing a high-frequency regeneration plant of pink, which comprises: 1, obtaining the protoplast of the pink; 2, subjecting the protoplast obtained by the step 1 to liquid shallow culture and enrichment culture to allow the protoplast to grow into regenerative cells and further into calluses; 3, subjecting the calluses obtained by the step 2 to bud initiation culture to grow seedlings on the calluses; and 4, subjecting a seedling obtained by the step 3 to rooting culture to grow the seedling into pink plant. The method of the invention adopts a protoplast culture technique and overcomes the technical drawback that it is difficult to obtain a regeneration plant of the pink from the protoplast of the pink in the prior art.

The invention provides a traditional Chinese medicine for treating bacterial vaginosis and a preparation method of the traditional Chinese medicine. Material medicines of the traditional Chinese medicine comprise: polygonum chinense, purslane, endive, Chinese osbeckia herb, horseweed herb, hairy euphorbia, wandering jew, amaranthus viridis, wideleaf osbeckia root, herb of sword brake, herba fibraureae recisae, dictamni cortex, lantana camara, cryptotaenia japonica hassk, senecio dianthus franch and root or leaf of redback christmashush. The traditional Chinese medicine disclosed by the invention is mainly used for clearing away heat and removing dampness, removing pattogenic heat from the blood and toxic material from the blood, sterilizing and relieving itching, has extract curative effect on bacterial vaginitis, is small in toxic and side effect, and does not easily have a relapse.

The invention discloses a method for cultivating polyploid dianthus caryophyllus germ plasm, which comprises the following steps: 2n gamete-containing diploid germplasm is cultivated which can be taken as a male parent to hybridize with a tetraploid female parent, the female parent is performed a castration and a bagging for 7 to 14 days during a full bloom stage, then an artificial pollination is performed, and the bag is removed and an ovary is taken off after pollinating for 15 - 45 days; the ovary is carried out a disinfection treatment and then cut off for taking off a white ovule, the ovule is performed an inoculation on an embryo culture medium for cultivating, the white ovule is grown to a black seed, a seed coat is cut off and a white embryo is taken off for continuous cultivating on a embryo culture medium, then a cultivation is conducted on a successive transfer culture medium for obtaining the polyploid dianthus caryophyllus germplasm. According to the invention, the diploid favorable gene and heterozygosity are successfully introduced in a tetraploid cultivation, which overcomes a problem of embryo abortion and greatly enriches a gene database of carnation genetic breeding, the invention is an effective approach of an objective trait introgression, and is beneficial to germplasmic introgression and gene flow of different ploidies, the hybridized progeny has more widely genetic variation and stable property as well.

The invention belongs to the technical field of cleaning agents and relates to a lagerstroemia indica cleaning solution for a refrigerator. According to the cleaning solution for the refrigerator, which is prepared by extracting lagerstroemia indica and other aromatic plant petal essence, peculiar smell and bacteria in the refrigerator are effectively removed; the lagerstroemia indica cleaning solution is prepared from the following raw materials in parts by weight: 5 to 10 parts of rose, 1 to 10 parts of osmanthus fragrans flower, 5 to 10 parts of violet, 5 to 8 parts of arabian jasmin, 10 to 30 parts of lemon, 10 to 30 parts of lagerstroemia indica, 5 to 10 parts of dianthus chinesis, 5 to 10 parts of lily of the valley, 5 to 10 parts of allium cepa, 8 to 12 parts of sansevieria trifasciata, 8 to 12 parts of chlorophytum comosum, 10 to 20 parts of aloe, 5 to 8 parts of turpentine oil, 8 to 15 parts of floor wax and 10 to 20 parts of lithopone, wherein the allium cepa has the effects of sterilizing and adsorbing peculiar smell; the arabian jasmin and the lemon have the effect of killing probiotics such as diphtheroid; the lagerstroemia indica, the dianthus chinesis, lily of the valley, the violet, the rose and the osmanthus fragrans flower can be used for inhibiting growth of bacteria; the aloe, the chlorophytum comosum and the sansevieria trifasciata have an effect of purifying air; a preparation process of the lagerstroemia indica cleaning solution is mature, safe and reliable; a formula of the lagerstroemia indica cleaning solution is reasonable; if the lagerstroemia indica cleaning solution is used for a long time, long-acting sanitation and cleanness of the refrigerator can be benefited, and an environment-friendly application environment is obtained.

The invention relates to rosaroxbunghii and dianthus chinensis tea and a making method for the same. Rosaroxbunghii is used as a raw material. The rosaroxbunghii and dianthus chinensis tea is characterized in that dianthus chinensis and stevia are also used as raw materials. The making method comprises the steps of (1) fruit selection and drying of rosaroxbunghii; (2) preparation of rosaroxbunghii particles; (3) screening and drying of dianthus chinensis and stevia; (4) preparation of dianthus chinensis tea particles and stevia particles; and (5) uniform mixing and packaging. According to the rosaroxbunghii and dianthus chinensis tea and the making method for the same, the formula is unique, reasonable compatibility of medicines is ensured, the nature of medicaments and the taste of tea supplement each other, blood sugar, blood lipid and blood pressure can be reduced, inflammation can be diminished, the rosaroxbunghii and dianthus chinensis tea has the healthcare effects of body nourishing and strengthening, stomach reinforcement, gut purging, constipation remission and the like, can be drunk by general populations, and is particularly suitable for diabetics and patients suffering from gastroenteritis, hyperglycemia, hypertension, hyperlipidemia and constipation, the types of the tea are rich, the making method is convenient and easy, great amounts of active ingredients are dissolved out, and the active ingredients can be easily absorbed by a human body after the tea is drunk.

The invention discloses dried asparagus and blueberry heat-clearing honey which is prepared from the following raw materials in parts by weight: 190-210 parts of osmanthus-scented honey, 10-15 parts of dried asparagus powder, 20-25 parts of blueberry, 7-8 parts of nutrition additives, 5-6 parts of shredded coconut stuffing, 14-15 parts of ham, 4-5 parts of Chinese hawthorn seed powder, 5-6 parts of purple-skin onion skin, 3-4 parts of blue-green algae powder, 4-5 parts of fresh minced fish, 3-4 parts of millet congee, 3-4 parts of biscuit, 5-6 parts of rum, 4-5 parts of loosestrife, 2-3 parts of tanakaea omeiensis, 2.1-2.3 parts of pink, 1.2-2 parts of pine bark, 2.8-3 parts of herba aristolochiae mollissimae and 2-3 parts of allii macrostemi bulbus. On the basis of remaining the natural nutritional ingredients of honey, a food with nutrition and health care effects is produced through scientific formula and advanced manufacturing process. After being eaten frequently, the effects of dispelling wind and eliminating dampness, activating blood and promoting granulation, descending qi and eliminating stagnation and breaking blood to stimulate the menstrual flow can be achieved, and thus the product is an optimal health food for modern women in pursuit of beauty.

The invention provides a dianthus chinensis cuttage breeding method. The dianthus chinensis cuttage breeding method comprises following steps of A, cutting selection; B, cuttage preparation; C, cuttage; and D, cuttage aftertreatment. According to the invention, semi-lignified shoots are employed as cuttings, compared with hardwood branches, the shoots are advantaged in that the rooting rate is increased; before cuttage, the lower ends of the cuttings are immersed into a growth regulator; after the cuttings are cut in the field, the growth regulator is added to the substrate around the cuttings drop by drop, by means of effect and influence of two-time growth regulator on the lower ends of the cuttings, the activity and the rooting rate of the cuttings can be greatly increased; water is sprayed to the cuttings intermittently, the humidity in the shading shed can be effectively maintained, and rootage of the cuttings can be promoted. By heating the seedbed, influence of diurnal temperature difference on the cuttings can be avoided, and the normal rooting environment of the cuttings can be guaranteed. In conclusion, the dianthus chinensis cuttage breeding method is simple in procedure, convenient to manage, short in period and high in survival rate, and a lot of seedlings can be bred within a short time.

The invention discloses a method for feeding frankliniella occidentalis indoors. The method comprises the following steps: inserting dianthus carryophyllus plants into a container filled with water; inoculating frankliniella occidentalis adults onto the plants; putting into a breeding cage; after oviposition, taking stems of the dianthus carryophyllus plants as explants; washing with running water; disinfecting with 75% ethyl alcohol and 0.1% mercuric chloride solution; washing with sterile water; then inoculating the explants onto an inducing medium; inducing multiple shoots in a tissue culture chamber under the culture conditions that the temperature is 25+ / -2 DEG C and the photoperiod is 16L:8D, wherein emerged adults are clean tested insects; transferring the tested insects onto dianthus carryophyllus tissue culture bottle seedlings; paving a layer of sterilized sand on the surface of culture medium in a tissue culture bottle; removing the adults 12 hours after the oviposition; putting back the bottle seedlings with eggs into the tissue culture chamber; and culturing until adults are emerged. The method has the advantages that plant tissue culture seedlings are adopted for feeding frankliniella occidentalis larvae and adults, the feed does not need to be replaced frequently, feeding steps are simplified, the feed is sufficient, the feeding efficiency is improved, and the survival rate is high.

The invention discloses a traditional Chinese medicine formula for treating eczematous dermatitis and a preparing method of traditional Chinese medicine. The traditional Chinese medicine comprises, by weight, the raw materials of 8g of Gynura pseudochina, 4 g of Flueggea virosa, 15 g of cajeput, 8 g of garden erphorbia herb, 6 g of nauclea officinalis pierre ex petard, 12 g of mottle leaves, 9 g of radix tinosporae, 9 g of tripterygium hypoglaucum hutch, 6 g of clamshell powder, 12 g of herba leucatis molloissimae, 8 g of sweet broomwort herb, 4 g of Sedum sarmentosum, 12 g of Polygonum posumbu, 15 g of Vitis flexuosa Thunb. leaves, 8 g of cynanchum amplexicaule, 10 g of Senecio dianthus Franch, 5 g of herb of three-leaved pronephrium and 8 g of Securidaca inapfondiculata Hassk. The cajeput is cajeput leaves. The traditional Chinese medicine formula for treating eczematous dermatitis is obvious in medicine effect, convenient to use, low in recurrence rate, free from side effect and low in price.

The invention relates to an irrigant for diminishing the inflammation of the wound, wherein the scientific combination of Paris polyphylla, Bletilla striata, Senecio scandens, Senecio dianthus Franch and borneol is adopted; pure Chinese medicinal herbs are used; the raw materials are accessible and convenient to use; and the irrigant has the functions of diminishing inflammation and promoting granulation, curing the trauma in vitro, infection of wounds, scalds, burns, deep-rooted boils and ulcers, washing the sutured wounds, preventing and curing the infection of wounds and promoting the healing of wounds.

The invention belongs to the technical field of medicines, and particularly relates to a method of preparing senecio dianthus franch total flavonoids and applications of the total flavonoids. The method includes a) drying senecio dianthus franch at 60 DEG C, and crushing to obtain crude powder, b) performing microwave-ultrasonic wave extraction, c) purifying with macroporous adsorptive resin, eluting with ethanol and collecting eluates, and d) concentrating and drying to obtain the total flavonoids. Pharmacodynamic evaluation proves that the extract has good oxidation resistance and obvious effects, can be used for preparing antioxidant medicines and has advantages of obvious curative effects, using convenience, and the like.

The present invention relates to a compound Chinese Mongolian medicine for effectively curing diabetes and its preparation method. It is made up by using Chinese medicinal materials of Chinese angelica root, typha pollen, leonurus, dianthus, bamboo leaf, black ant, seal's genitals, sea horse and dragon's blood as raw material and adding carboxymethyl cellulose sodium and magnesium stearate as excipient through a certain preparation process.

The invention discloses a traditional Chinese medicinal composition used for treating marek's disease of chicken and a preparation method thereof. The traditional Chinese medicinal composition is prepared from the following raw herbal materials: cynanchum thesioides, mylabris, persicaria perfoliata, dichroae radix, myrsinaceae, gelidium amanisi, trametes robinioplila, senecio dianthus franch, luffa water, vervain, veronicastrum herb, tree beautyberry roots and leaves, selaginella tamariscina, diuranthera minor root, wikstroemia dolichantha root, balanophoora involucrate hook, gomphocarpus fruticosus, polygonatum kingianum, sawtooth oak bark, roxburgh rose, Chinese gall, polygonatum odoratum, rhizoma imperatae, smoked plum, salviae miltiorrhizae, embelia laeta and panax notoginseng. The traditional Chinese medicinal composition used for treating marek's disease of chicken has the beneficial effects that the traditional Chinese medicinal composition disclosed by the invention can be used for treating the marek's disease of chicken and enhancing operation of normal functions of viscera of the chicken, and is short in treatment course, quick in effect, low in cost, purely natural and free of pollution.

The invention relates to a pink male sterile line transformation and a cultivation method for the breed and coenospecies, belonging to the technical field of plant cultivation. The method uses the existing pink male sterile line as a female parent and at least two pink self compatible lines as male parents, and biparental cross is carried out respectively with the pink male sterile line as the female parent; a single plant mostly close to the economic character of the male parent is selected from the later generations of the biparental cross as the female parent and then backcrossed with the original male parent; the backcrossing is repeated, and after at least five generations of backcrossing and transformation, the pink male transformation sterile line is obtained. The pink male transformation sterile line obtained by adopting the method of the invention has the advantages of stable male sterility, better combining ability, and the like; by means of multiple self-crosses and line-crosses, most combinations show the heterobeltiosis of disease resistance, growing luxuriance, flowering branch increasing, colored flower, and the like; the labor and cost can be obviously saved.

The invention belongs to the technical field of gene engineering of decorative plants, and particularly relates to a method for improving dianthus conversion rate based on the tobacco rattle virus silencing system. The silencing system comprises a PDS gene sequence shown in SEQ ID NO: 1, a specific primer is designed according to the gene fragment of the dianthus, a 305bp cDNA fragment is amplified in the dianthus by PCR, the obtained fragment is connected to a TRV2 carrier, and a recombinant expression carrier TRV2-DcPDS is constructed. The dianthus is infected by using an agrobacterium-mediated mechanical damage method, and the gene function is identified by using the recombinant expression carrier TRV2-DcPDS to transform the dianthus by using a transient transformation method to inducesilencing of the gene of the dianthus. The method can be used for fast function identification and transgenic operation of the dianthus gene.