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Tissue culture method of dianthus deltoids

A technology of girl dianthus, cultivation method, applied in gardening methods, botanical equipment and methods, horticulture and other directions, can solve the problems of degradation, quality of disease infection, less seedlings in seedling cultivation time, etc.

Active Publication Date: 2014-06-04
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial propagation of dianthus still mainly adopts seeding method and cutting method, the number of reproduction is limited, the time is long, and it is easy to cause adventitious bud glass flower, disease infection and quality degradation
At present, there are few studies on tissue culture propagation that can be used to solve the problems of shortening seedling cultivation time, industrial production, vitrification of seedlings, and renewal and rejuvenation. Therefore, it has become one of the urgent tasks for scientific researchers to carry out in-depth research on tissue culture of dianthus.

Method used

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  • Tissue culture method of dianthus deltoids
  • Tissue culture method of dianthus deltoids
  • Tissue culture method of dianthus deltoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1. The influence of the selection and operation method of the girl carnation explant on the induction of adventitious buds

[0034] The dianthus used in the experiment was collected from the experimental nursery of the Drought Resistant Plant Research Institute of Hexinyuan Mengcao Drought Resistant Greening Co., Ltd. in Inner Mongolia. According to the progress of the experiment, samples were taken at different times and supplemented at any time. Harvest the stem tips of young dianthus shoots about 0.5-1.0cm long, wash them with running water, soak them in 70% alcohol for 10 seconds, then disinfect them with 0.1% mercury liter for 5 minutes, rinse them twice with sterile water, 5 minutes each time , inoculated on the improved primary medium (MS) to establish a clone of test-tube plantlets. Select the clustered buds of about 10 days of subculture, cut off the base of the clustered buds; take the shoot tips of the clustered buds, about 0.5-1.0 cm in length, as...

Embodiment 2

[0041] Example 2. Screening of optimal medium for each stage of girl carnation tissue culture

[0042] 1. Statistical calculation formula of screening effect

[0043] Survival rate = (number of viable explants / total number of inoculated explants) × 100%

[0044] Primary bud induction rate=(number of explants that germinated / total number of inoculated explants)×100%

[0045] Multiplication multiple of subculture = (number of clustered buds induced / total number of buds inserted into a single plant) × 100%

[0046] Rooting rate = (number of explants with differentiated roots / total number of inoculated explants) × 100%

[0047] 2. Selection of the best medium for each stage of Dianthus tissue culture

[0048] MS was used as the basic medium, 3.5g / L carrageenan was used as the solidifying agent, 3% edible sugar was used as the carbon source instead of sucrose, tap water was used instead of distilled water, and different ratios of cytokinin and auxin were added to the culture m...

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Abstract

The invention relates to a tissue culture method of dianthus deltoids. The tissue culture method comprises the steps of taking stem apex of dianthus deltoids twigs as an explants; subjecting the stem apex to sterilization; and culturing by inoculating the stem apex to a primary induction medium composed of MS, 0.01 mg / L 6-BA and 0.05 mg / L NAA, a subculture medium composed of MS, 0.01 mg / L 6-BA and 0.05 mg / L NAA and a rooting medium composed of 1 / 2 MS and 0.05 mg / L IBA successively under culture conditions that a culture temperature is 25+ / -1 DEG C; illumination intensity is 2,500 Lx and light is supplemented for 4 hours / day at night except natural light in the daytime. The culture method can obtain large-scale indoor bred dianthus deltoids seedlings in a short time, has high production rate and has potential ecologic benefits and social benefits.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for tissue culture of dianthus, which is particularly suitable for dianthus, and is far superior to the tissue culture methods of other dianthus, including explants of dianthus The selection of the subculture medium and the number of culture generations in the shoot tip tissue culture, the selection of the culture conditions in the tissue culture process, etc. Background technique [0002] Dianthus is a perennial grass flower cultivated as one or two years. It is an important ornamental flower because of its rich color, long flowering period and extensive cultivation and management. There are more than 600 species in the world, widely distributed in the northern temperate zone, mainly in Eurasia, especially in the Mediterranean region, and a few in North America and North Africa. According to "Flora of China", there are 17 species, 1 subspecies, and 9 vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马怀林王召明高秀梅田志来
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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