74 results about "Carnation" patented technology

The invention discloses health tea which is manufactured by mixing 0 to 80 weight parts of herbal tea, 0 to 50 weight parts of multicolor tea and 0 to 10 weight parts of medicinal and edible nourishing medicinal material, wherein the herbal tea is manufactured by adopting one or more from prunus persica, flos rosae rugosae, tulipa, rosa chinensis, chrysanthemum, aloe, apple flower, hibiscus sabdariffa, jasminum sambac, lemon slices, lavandula pedunculata, lilium, lemon grass, prunus mume, trollius chinensis bunge, superslim, sterculia lychnophora, fiveleaf gynostemma herbs, stevia rebaudiana, radix notoginseng, osmanthus fragrans, jade beauty, stellaria alsine grimm, dianthus caryophyllus, apocynum venetum, cordyceps, flos lonicerae, paeonia lactiflora and arctium lappal; the multicolor tea is manufacrured by adopting one or more from green tea, black tea, dark green tea and white tea; and the medicinal and edible nourishing medicinal material is manufactured by adopting one or more from panax quinquefolius, radix glycyrrhizae, dendrobium nobile, salvia miltiorrhiza, cornus officinalis, radix pseudostellariae and codonopsis pilosula. The health tea has the advantages that fragrance, sweetness and mellowness of scented tea are maintained, a health care function of the nourishing medicinal material is further fulfilled, and besides, tea soup is rich in taste and is sweet, refreshing and fragrant.

The invention provides carnation high-efficiency rootone which is characterized by containing the following materials according to weight part: 10 parts of rhodofix, 1-2 parts of seradix, 80-120 parts of mildothane, 15-30 parts of streptomycin and 6000-6400 parts of talcum powder. In the invention, aiming at the problems of root rot and stem rot of the carnation easily harmed by epiphyte and bacteria and being combined with the characteristics that roots produced by rhodofix NAA induction are less in quantity and thick and adventitious roots produced by seradix IBA action are thin and long, NAA and IBA are mixed in the rootone, and meanwhile, mildothane used for sterilizing and agricultural streptomycin are added; therefore, the rooting ratio of the carnation is high, the growing roots are long and thick, the antibiosis and disease-resisting capacity is enhanced, and the root rot ratio is greatly reduced, and the invention has the advantages of tidy rooting, high field production survival rate, convenient usage, low cost, good effect, and the like and is very convenient for the growth of the carnations.

The invention provides a method for cryopreservation and plant regeneration of Dianthus caryophyllus shoot tips. Growth-restored regenerated Dianthus caryophyllus plants are obtained through preculture, loading, dehydration, freezing, thawing, restoration culture and the like. By adopting the method provided by the invention, the regeneration rate of Dianthus caryophyllus can reach 52.4 percent, the device for the vitrification cryopreservation of the Dianthus caryophyllus shoot tips is simple, the process is simplified, the operation is easy, the freezing effect is good, the restoration growth of the preserved Dianthus caryophyllus is good, the hereditary variation probability is small and the method is an effective way for the in vitro preservation of Dianthus caryophyllus germ plasm resources.

The invention relates to an internal standard gene suitable for detecting a transgene carnation exogenous gene. According to the three standards of the intraspecies non-specificity, the interspecies specificity and the single-copy or low-copy number of the internal standard gene and by the analysis of biologic informatics and the experimental verification of molecular biology, the invention searches out an ANS gene conforming to the standards to be used as the internal standard gene for the qualitative and quantitative PCR detection of transgene carnation in the carnation. A qualitative PCR primer is ANS-F1 / ANS-R1, a quantitative PCR primer is ANS-F2 / ANS-R2, and a quantitative PCR probe is ANS-probe. The ANS gene is used as the internal standard gene for the qualitative and quantitative PCR detection and the copy number analysis of the transgene carnation.

The invention relates to a fresh cut flower preservative made by adopting a surfactant and a preparation method thereof, belonging to the technical field of preservation of fresh cut flowers. The fresh cut flower preservative consists of sucrose, 8-hydroxyquinoline (8-HQ) and the surfactant of Tween (polyoxyethylene sorbitan fatty acid ester). The surfactant (Tween) is taken as the raw material, and the chemical mixing treatment is further carried out for preparing the fresh cut flower preservative which can play the preservation role for the fresh cut flowers (such as Dianthus Caryophyllus and the like). Preserving solution containing the surfactant can well defer the water loss of flower branches of the fresh flowers, keep a certain expansion pressure of the flower branches, and achieve the purpose of prolonging the service life of bottle inserts, and the service life of the bottle inserts of the fresh cut flowers can be prolonged by 9-12 days in compared with a control group.

The invention discloses a method for rapidly breeding potted carnation through tissue culture. The method comprises the following steps of: cutting an explant and sterilizing; carrying out primary culture, wherein a primary culture medium comprises MS (Murashige and Skoog), 1.5mg / L of 6-BA (6-Benzylaminopurine), 0.2mg / L of NAA (Naphthalene Acetic Acid), 30g / L of sucrose and 8g / L of agar; carrying out subculture, wherein a subculture medium comprises MS, 0.8mg / L of 6-BA, 0.2mg / L of NAA, 30g / L of sucrose and 8g / L of agar; carrying out rooting culture, wherein a rooting culture medium comprises MS, 0.2mg / L of NAA, 0.1mg / L of IAA(Indole Acetic Acid), 30g / L of sucrose and 8g / L of agar; and washing the culture mediums away and then holding the temperature and humidity for seedling hardening and transplanting. The method is simple and easy to operate, simple in procedure and high in working efficiency, and is capable of effectively holding excellent properties of parents. According to the method, the differentiation rate of a tissue culture seedling is increased. The method has the characteristics of high multiplication speed, high rooting rate, high survival rate and low cost.

The present invention discloses a method for detoxification tissue culture and rapid propagation of dianthus caryophyllus. The method comprises steps such as a heat treatment, explant surface disinfection, stem apex peeling, stem apex culture and the like, wherein lateral bud growing between the dianthus caryophyllus plant leaf axils is adopted as the explant, the sufficient sterile explants can be obtained through match use of ethanol and sodium hypochlorite, the explant can be subjected to direct differentiation on an induction culture medium to obtain the regeneration plant, and the regeneration rate of the plant achieves 67%, wherein the induction culture medium comprises 1 L of an MS culture medium, 25-35 g of sucrose, 5-8 g of agar, 1-3 mg of IAA, 0.5-1.5 mg of NAA, and 0.5-2 mg of ZT.

The invention discloses a natural toxin-expelling young-keeping herbal tea. The herbal tea uses honey and 8 purely natural flowers and plants consisting of ginseng flower, trollflower, carnation, rose, roselle, shaddock flower, red date and wolfberry as raw materials and does not contain any artificial auxin component. The plurality of effective flower and plant components of the herbal tea can adjust endocrine, eliminate toxins in the body, regulate the exterior by tonifying the interior, remove foreign substances which are not inherent in the skin, purify the body and the heart, maintain beauty and keep young, and the herbal tea is convenient to drink and especially caters for taste of females. A great number of people taste the herbal tea by themselves, and the herbal tea wins public praise. The herbal tea has the advantages of scientific and reasonable compatibility, simple preparation, low cost and convenience in drinking.

The invention discloses a method for preparing the filial generation of dianthus caryophyllus and dianthus chinensis, which comprises: 1, obtaining the protoplast of the dianthus caryophyllus and the dianthus chinensis respectively; 2, performing protoplast asymmetric fusion of the protoplast of the dianthus caryophyllus and the protoplast of the dianthus chinensis to obtain fused protoplast; and 3, culturing the fused protoplast to grow the protoplast into a hybrid plant. The invention provides the first method suitable for culturing the filial generation of the protoplast of the dianthus caryophyllus and the dianthus chinensis, overcomes the drawback that it is difficult to obtain the filial generation of the dianthus caryophyllus and the dianthus chinensis in the prior art and, by optimizing cross conditions, cultures novel filial generation which has good plant shape, high ornamental performance and high quantity of mu plant rate for the first time.

A method of transforming a carnation (Dianthus L.) plant genome with a DNA molecule. The method comprises (a) preparing stem explants from carnation cuttings; (b) wounding the explants by microprojectile bombardment; (c) cocultivating the wounded explants with Agrobacterium comprising the DNA molecule under defined conditions of exposure to dark followed by light; (d) excising shoots from the cultivated wounded explants and removing the leaves from the shoots; and (e) culturing the leaves to obtain transgenic shoots transformed with the DNA molecule. Also disclosed are a rolC-transgenic carnation with improved agronomic traits and enhancement of flower fragrance by antisense suppression of the flavonoid gene fht.

The invention discloses lily and strawberry health-care rice and a processing method of the lily and strawberry health-care rice. The lily and strawberry health-care rice consists of the following ingredients in parts by weight: 10 to 15 parts of lilies, 8 to 12 parts of hibiscus rnanihot flowers, 20 to 30 parts of strawberries, 20 to 30 parts of mulberries, 8 to 15 parts of grapestone powder, 150 to 200 parts of polished round-grained rice, 60 to 80 parts of buckwheat, 5 to 10 parts of egg albumen powder, 10 to 14 parts of honey, 1 to 2 parts of syzygiumgrijsii, 1 to 2 parts of elecampane, 2 to 44 parts of apple flowers, 2 to 4 parts of globe amaranth, 2 to 4 parts of dianthus caryophyllus, 10 to 20 parts of rice wine and 8 to 12 parts of food additives. The lily and strawberry health-care rice has the advantages that the eating is convenient; the nutrition is rich; nutritional ingredients such as the strawberries and the lilies are merged; the fragrance, the silky smoothness, the sweetness, the softness and the tenderness are achieved; and after the lily and strawberry health-care rice is eaten for a long time, the effect of prompting the digestion can be achieved, the immunologic function of the body is improved, and meanwhile, the effects of moistening and whitening the skin, calming the nerves and realizing beautification are achieved.

The invention provides a pesticide for preventing and treating the root knot nematode disease, and belongs to the technical field of pesticides. The pesticide for preventing and treating the root knot nematode disease is prepared from, by weight, 10-30 parts of Chinese honeylocust fruits, 10-30 parts of rhizoma arisaematis, 10-30 parts of manyleaf paris rhizomes, 10-30 parts of lilac, 5-20 parts of cortex cinnamomi, 5-20 parts of dianthus caryophyllus, 5-20 parts of officinal magnolia bark, 5-15 parts of holy basil, 3-12 parts of cacumen biotae, 3-12 parts of pinellia ternate, 3-12 parts of banana roots, 1-10 parts of liquorice roots, 1-5 parts of pyroligneous liquid and 1-5 parts of biological fungicide. The biological fungicide is prepared from, by mass, 30%-40% of bacillus cereus and 60%-70% of trichoderma harzianum. The pesticide is obtained through scientific compounding, the raw materials cooperate to prevent and treat the root knot nematode disease, the pesticide effect can reach 97% or above, the effect is stable, and pesticide resistance is avoided.

The invention relates to cultivate medium composite, used for induce germ at bamboo. Wherein, it adds 6-BA at 0.05-1.2mg / L into MS cultivate medium, to effectively induce germ, while the MS added with 6-BA 1mg / L has best effect. The invention points out that growth adjuster 6-BA can accelerate the germ induction of bamboo. The invention also provides an application of said composite to cultivate the bamboo organism.

The invention discloses a paraffin slicing method for large explant leaf-buds and flower-buds of carnation, comprising steps as follows: material selection and fixation, cleaning and dyeing, dehydration, transparency, paraffin impregnation and embedding, slicing, section flatting and section sticking, deparaffinating and section sealing, etc. By the method of the invention, problems such as incomplete and discontinuous sections, cavity and the like due to overlarge explant leaf-buds and flower-buds of carnation can be effectively solved. Various growth stages of tissues can be greatly fixed, and the whole growth process of leaf buds and flower buds can be observed. In addition, the method is simple to operate and has good development and application value.

The invention discloses a tissue culture and rapid propagation method for dianthus caryophyllus L. The dianthus caryophyllus L belongs to caryophyllaceae dianthus herbaceous perennial flowers, has the advantages of high productbility, good ornamental value, easiness in storage and transportation, and the like, and is one of main culture varieties of commercial cut-flowers cultured most widely in the present world. A large number of high-quality seedlings can be obtained by plant in-vitro culture, the propagation coefficient is greatly increased, viruses carried in a plant body are reduced, the stress resistance of the whole plant is improved, the limitations of environmental conditions of seasons are avoided, and the defects of conventional cutting propagation are made up through the breeding mode. A lateral bud of dianthus caryophyllus L is taken as an explant, and a dianthus caryophyllus L in-vitro replanting plant is successfully obtained by adventive bud induction culture, multiplication culture, rooting culture, acclimatization and transplantation, and the like, a dianthus caryophyllus L tissue culture and rapid propagation system is established, and the large-scale production of dianthus caryophyllus L tissue culture seedlings is promoted.

The invention discloses a traditional Chinese medicine unguent for treating the trauma accumulated toxin ulceration pain disease and a preparation method thereof. The traditional Chinese medicine unguent for treating the trauma accumulated toxin ulceration pain disease is prepared from, by weight parts, rhizoma coptidis, radix ampelopsis, milkvetch roots, cutch, dianthus caryophyllus, dandelions,herba portulacae, vaseline, 5-10 parts of dragon's blood, cuttle-bones, herba epimedii, honeysuckle flowers, snake oil, badger fat, fructus forsythiae, radices lithospermi, forged calamine, gypsum rubrum, gypsum, radix sophorae flavescentis, amur cork tree bark, radix angelicae, the root bark of white mulberry and pseudo-ginseng. The traditional Chinese medicine unguent has the advantages that theunguent has a remarkable treatment effect on bedsores, angiitis ulceration, acne, incised wounds and postoperative breast cancer pains and ulceration, pain relieving takes effect rapidly, use is convenient, the treatment cost is low, no scar is left after healing is achieved, and the traditional Chinese medicine unguent is a choice drug for treatment and is suitable for treating traumas in hospitals and homes.

The invention relates to cultivate medium composite, used for induce germ at bamboo. Wherein, it adds IAA 0.1-1.0mg / L or IBA 1.0mg / L into semi-density MS cultivate medium. The invention points out that growth adjuster IAA can accelerate the germ induction of bamboo. The invention also provides an application of said composite to cultivate the bamboo organism.

The invention belongs to the field of plant tissue culture, relating to a method for regenerating whole plant by in vitro propagation of flower and plant dianthus caryophyllus. The method comprises the following steps: a) inoculating the bud of the sterilized dianthus caryophyllus into an embryonic callus induction culture medium to induce the callus; b) transferring the callus with favourable development in step a) onto the embryonic callus induction culture medium to be proliferated; c) inoculating the callus with favourable development in step b) onto the embryonic callus induction culture medium, differentiating the induced somatic embryo to obtain a mature somatic embryo; and d) transferring the obtained mature somatic embryo onto the somatic embryo seedling culture medium to be regenerated into a whole plant. The explant adopted by the invention is not limited by seasons and can culture tissues and cells annually; in-vitro dianthus caryophyllus can be obtained by continuously differentiating the embryonic callus and has favourable differentiation effect; the embryonic callus of the dianthus caryophyllus can be successively stored for a long time and can keep the capability of being further differentiated into plantlets.

The invention discloses a method for cultivating polyploid dianthus caryophyllus germ plasm, which comprises the following steps: 2n gamete-containing diploid germplasm is cultivated which can be taken as a male parent to hybridize with a tetraploid female parent, the female parent is performed a castration and a bagging for 7 to 14 days during a full bloom stage, then an artificial pollination is performed, and the bag is removed and an ovary is taken off after pollinating for 15 - 45 days; the ovary is carried out a disinfection treatment and then cut off for taking off a white ovule, the ovule is performed an inoculation on an embryo culture medium for cultivating, the white ovule is grown to a black seed, a seed coat is cut off and a white embryo is taken off for continuous cultivating on a embryo culture medium, then a cultivation is conducted on a successive transfer culture medium for obtaining the polyploid dianthus caryophyllus germplasm. According to the invention, the diploid favorable gene and heterozygosity are successfully introduced in a tetraploid cultivation, which overcomes a problem of embryo abortion and greatly enriches a gene database of carnation genetic breeding, the invention is an effective approach of an objective trait introgression, and is beneficial to germplasmic introgression and gene flow of different ploidies, the hybridized progeny has more widely genetic variation and stable property as well.

The invention discloses a compound health care pressed salted duck with a nourishing function and a processing method thereof. The compound health care pressed salted duck is prepared from the following raw materials in parts by weight: 1000-1200 parts by weight of eviscerated duck, 40-60 parts by weight of tremella, 100-120 parts by weight of Chinese yam, 60-80 parts by weight of waxy corn, 30-50 parts by weight of sarcodia montagneana, 20-40 parts by weight of crosne, 20-40 parts by weight of salt, 2-3 parts by weight of ricebird flower, 1-2 parts by weight of codonopsis pilosula, 1-2 parts by weight of dianthus caryophyllus, 2-3 parts by weight of lotus fruit base, 2-3 parts by weight of cacumen biotae, 2-3 parts by weight of colquhoumia, a proper amount of red wine and a proper amount of nutrient solution. The compound health care pressed salted duck with the nourishing function, processed by adopting the processing method, is fresh, elegant and mellow, soft and tender in meat, special in flavor, rich in nutrients, high in health care value, and convenient to eat, and has functions of tonifying middle-Jiao and Qi, tonifying the spleen and the stomach, clearing liver and improving vision, and enriching Yin and nourishing kidney; the nourishing function is improved.

The invention discloses a method for cloning unknown flanking sequences of a PS1 gene 5' of dianthus caryophyllus, belonging to the technical field of bioengineering. According to the method, five degenerate primers and two specific primers are designed according to homologous sequences, and the unknown flanking sequences of the PS1 gene 5' of dianthus caryophyllus can be obtained by adopting nested PCR (Polymerase Chain Reaction) amplification. The method disclosed by the invention has the characteristics of simplicity and convenience in operation, few operating restrictions, long cloned fragments, strong pertinence, low cost and the like.

The present invention relates to one specific flower promoter obtained through cloning carnation CMB2 gene, and the expression box and plant expression vector of the promoter. The specific flower promoter can drive the high efficiency specific expression of target gene in the flower organ, especially in petal and filament, of transgenic plant without expression in root, stem, leaf and other vegetative organs. The promoter of the present invention may applied in flower gene engineering to cultivate novel beautiful durative strain of carnation and other flowers.

The invention provides a botanical pesticide for preventing and treating diseases and insect pests of tea trees, belonging to the technical field of pesticides. The botanical pesticide is prepared from the following raw materials in parts by weight: 10-30 parts of Chinese honeylocust fruit, 10-30 parts of rhizoma arisaematis, 10-30 parts of manyleaf paris rhizome, 10-30 parts of syringa oblate, 5-20 parts of cassia bark, 5-20 parts of dianthus caryophyllus, 5-20 parts of cortex magnoliae officinalis, 5-15 parts of holy basil, 3-12 parts of cacumen platycladi, 3-12 parts of rhizoma pinelliae, 3-12 parts of banana root and 1-10 parts of liquorice. By virtue of scientific compounding of Chinese herbal medicines, a synergistic interaction effect is achieved, the anti-microbial and insecticidal capacities of the pesticide are remarkably enhanced, the anti-microbial and insecticidal spectra are relatively wide, and the possibility of generating drug resistances of pests is low.

The invention discloses a cultivation method of dianthus caryophyllus. The cultivation method comprises the following steps of S1, preparation of a matrix, S2, cuttage of seedlings, S3, pinching careand S4, disease and insect pest prevention and treatment. The cultivation method of the dianthus caryophyllus has the advantages that broken ceramsite and turf are adopted as the cultivation matrix sothat the cultivation matrix can obtain a good cultivation effect, the growth and development of the dianthus caryophyllus are facilitated, a used nutrient solution and the reasonable dilutability ofthe nutrient solution provide trace elements required for the growth of plants, and the dianthus caryophyllus is cultivated by the method to greatly increase the chlorophyll content and ear harvestingquantity of dianthus caryophyllus blades.

Provided is a technology for making a dianthus caryophyllus crystal flower. All petals of dianthus caryophyllus are pushed aside and separated through tweezers, so that the whole flower is kept in a fully spread state; under the circumstance that the original shapes of the petals are not damaged, drying is carried out through the method that embedding drying by drying agents and drying by a microwave oven are combined, the drying efficiency can be greatly improved through the method, and the dried petals are made into a stereoscopic dry dianthus caryophyllus flower; the surface of the dried stereoscopic dianthus caryophyllus flower is coated with a layer of protecting film through crystal glue, water factors in the air are prevented from influencing the flower, and therefore the stereoscopic dianthus caryophyllus crystal flower which is stable in flower form, not liable to discoloration caused by moistening and high in ornamental value and has certain crystal texture glossiness is obtained. The technology is simple and convenient to implement, low in investment, short in period, suitable for dianthus caryophyllus in various colors and capable of being used for do-it-yourself (DIY) making of dianthus caryophyllus artware, the requirement of customers for DIY making of dried flowers and crystal flowers of dianthus caryophyllus is met, and the commercial value of the dianthus caryophyllus on the market is increased.

The invention discloses a biofilm culturing disinfectant, which is prepared from the following raw material components: 40 to 60g of violet, 24g to 36g of carnation, 12g to 18g of amber and 64g to 96gof west vanilla; 24 mL to 36 mL of peony essential oil, 40 mL to 60 mL of cornus officinalis and 240 mL to 360 mL of hydrogen peroxide silver ion composite liquid; and the content of the Jinkang stone water as 900L-1000L. The invention has the beneficial effects that the adhesive force of the hydrogen peroxide silver ion composite disinfectant to the surface of an object is increased, the concentration of sterilization and disinfection can be kept for a long time after spraying, the virus killing time is greatly prolonged, and therefore propagation of novel coronaviruses is better controlled;due to the fact that sterilization time efficiency of one-time spraying is increased, the number of disinfection times can be reduced, and labor cost is saved.

The invention discloses fermented rich-selenium ficus carica pellets prepared from the following raw materials: ficus carica, calystegia hederacea, roselle, sweet osmanthus flowers, carnation, stock gillyflower, camomile, ficus carica leaves, piscidia leaves, futokadsura stem, selenium-rich glutinous rice, lactosucrose, lactobacillus, vitamin C and lysozyme. The fermented rich-selenium ficus carica pellets provided by the invention are low in price, simple in preparation, rich in nutrition, aromatic in flavour, red and bright in color, palatable in sourness and sweetness, rich in organic selenium and comprehensive in healthcare function, is capable of enhancing the immunity, promoting metabolism, protecting heart and cerebral vessels, promoting gastrointestinal functions, resisting oxidization, resisting aging and resisting cancer; after being fermented through lactobacillus, the fermented rich-selenium ficus carica pellets are slightly sour in taste, relatively more micromolecule nutritive and fragrant ingredients are produced, nutrient absorption is promoted, and the fermented rich-selenium ficus carica pellets can keep natural and aromatic flavour for a long time; the selenium-rich glutinous rice is capable of enriching nutrition, increasing the organic selenium content, and keeping the complete shape and softness of the product; lysozyme is capable of increasing nutrition, naturally resisting bacteria and ensuring the tissue uniformity.

The invention relates to the technical field of plant rapid propagation, and particularly discloses a high-retainment dianthus caryophyllus seedling propagation method which comprises the following steps: inoculating a dianthus caryophyllus branch serving as an initial explant onto a fixed bud induction culture medium, and performing adventitious bud induction pre-culture and adventitious bud induction culture to obtain clustered buds. Test results show that the adventitious buds can be directly induced to form buds only by about 2 weeks, the growth speed is remarkably higher than that of callus induced bud formation, and the direct induced bud formation has good hereditary stability and has good application and popularization prospects.

The invention provides a tissue culture and rapid propagation method of a carnation. The method comprises the following steps: cutting the hypogenetic tissue culture carnation seedlings into fragments; putting the fragments into different culture mediums to cultivate for 15-30 days alternately, wherein each time, the culture temperature is 22-26 DEG C, the light intensity is 2000-3000lx and the light duration is 10-14 hours per day; cutting the proliferous tissue culture seedlings into fragment and putting the fragments into the next culture medium for further cultivation to obtain well-developed tissue culture seedlings. The tissue culture and propagation method provided by the invention can effectively solve the problems that during the tissue culture and propagation of the carnation, due to the reason of variety or increase of subculture times, the stems of the tissue culture seedlings are thin and delicate, the propagation coefficient is decreased, growth is slow, yellowing, vitrifying, weak propagation ability or no propagation is generated, and the like. The tissue culture and rapid propagation method of the carnation has important significance on carnation germplasm resource preservation and industrialized production of good seedlings.