76 results about "Epiphyte" patented technology

The invention relates to procedures of soil disinfection to prevent and wipe out facility vegetable soil-borne diseases, operation steps are as follows: smoothing and watering the soil during the season with high temperature and good illumination to make sure relative humidity of the soil is 65%-80%, liming calcium cyan amide equably 6-100kg per 1000 m2 two days after watering, ploughing under to 30-40cm to make sure that the calcium cyan amide and the soil are mixed equably, confining the surface of the soil with transparent film and the booth or glasshouse totally for 12-20 days. The invention is mainly used for preventing and curing facility vegetable soil-borne diseases of cucumber, tomato, hot pepper, eggplant and so on, caused by pathogeny epiphyte, bacteria and wireworm. The invention prevents and cures soil-borne diseases greatly and duratively, offering vegetables nitrogen, decrease the accumulation of nitrate, accelerating plant growth. Said procedures of soil disinfection are novel to operate with easy steps, low cost, high efficiency and safety, significant economic benefit.

Soil-free living roof and wall systems comprising a living layer of Bryophytes, Lichen, And Vascular Epiphytes (BLAVE) on the surface of a thin, light-weight, flexible, fire-resistant mat of mineral wool having a density in the range of about 6-12 lbs / cu ft and thickness in the range of about ⅜″ to about ¾″. Mats are provided in modular units on the order of 2′×4′; no irrigation or growth medium is used. The mats are adhered to a roof or wall with adhesive or mastic, or may be secured with fasteners. In the preferred embodiment, modular units include flexible mineral wool mats over a base sheet of non-woven plastic fibers, on the order of from 2-4 mm thick, and a wide-mesh plastic netting over the BLAVE layer, sewn through to the base layer. Methods of mat module manufacture, innoculant mix production, installation and cultivation / propagation of BLAVE are disclosed.

The invention discloses a method for culturing an orchid and a special bacterium thereof. The name of the bacterium of the invention is GDB181 the preservation number of which is CGMCCNo.2574. The method of the invention includes the steps for together culturing the bacterium of the invention and the orchid. The bacterium of the invention is applicable for the root culturing of an orchid bacteria and builds an epiphyte bacterium with excellent root which effectively coexists with the orchid. The method of the invention for culturing the orchid can better control the inoculation amount and can fix the inoculation position, thus fully ensuring the tissue culture seedling of the orchid to build the coexistence relation with the epiphyte bacterium with excellent root, simultaneously simplifying the inoculation steps and reducing the inoculation times. Permanent effect can be achieved by being inoculated for once, thus avoiding the dead seedling phenomenon caused by a plurality of manual inoculations, thereby improving the survival rate of transplanting and obtaining the root orchid seedling which grows strongly. Therefore, the method for culturing bacterium, the culture medium of the bacterium and the culture medium of the orchid of the invention has important economic values and is applicable for popularization and application.

The invention relates to synthetic antibacterial peptide comprising polypeptide selected from SEQ ID NO:1-SEQ IDNO:13 amino acid sequences, and polypeptide of a functional equivalent obtained from the synthetic antibacterial peptide through cyclization, N-terminal and C-terminal modification, translation from L-type amino acid to D-type amino acid, and sequence terminal deletion. The synthetic antibacterial peptide is synthesized by a solid phase chemical method. The synthetic antibacterial peptide has broad-spectrum killing activity to gram negative bacteria, gram positive bacteria and epiphytes, has stronger bactericidal activity than natural antibacterial peptide, has no toxic action on animal and plant cells, and can be used for preparing medicaments for curing infectious diseases.

A dendrobium candidum planting method specifically includes the steps that dendrobium candidum seedlings are planted on tree species with longitudinal grooves in the surface of bark; before planting, the roots of the dendrobium candidum seedlings dip in a solid nutrient solution prepared through a high-water-absorbent material and a Jin Weiguo effective foliar fertilizer produced by the America Grow More Inc; during planting, the dendrobium candidum seedlings are bound on trunks through binding strips manufactured through shading nets so that the dendrobium candidum seedlings can be attached to the trunks. According to the method, the trunks serve as epiphytes, tress are used for shading, plastic greenhouses and other infrastructure do not need to be built, substrates are not used, cost is reduced by nearly a half, and environment is not influenced. Meanwhile, dendrobium candidum products cultivated through live trees are more ecological, higher in polysaccharide content and higher in quality. Dendrobium candidum is planted on the trees, does not occupy fertile farmland suitable for grains and does not occupy forest land suitable for the trees, and comprehensive benefits of the forest land can be better achieved. Most importantly, the live trees are planted in the wild, restoration of wild populations is facilitated, and sustainable development is achieved.

The invention provides carnation high-efficiency rootone which is characterized by containing the following materials according to weight part: 10 parts of rhodofix, 1-2 parts of seradix, 80-120 parts of mildothane, 15-30 parts of streptomycin and 6000-6400 parts of talcum powder. In the invention, aiming at the problems of root rot and stem rot of the carnation easily harmed by epiphyte and bacteria and being combined with the characteristics that roots produced by rhodofix NAA induction are less in quantity and thick and adventitious roots produced by seradix IBA action are thin and long, NAA and IBA are mixed in the rootone, and meanwhile, mildothane used for sterilizing and agricultural streptomycin are added; therefore, the rooting ratio of the carnation is high, the growing roots are long and thick, the antibiosis and disease-resisting capacity is enhanced, and the root rot ratio is greatly reduced, and the invention has the advantages of tidy rooting, high field production survival rate, convenient usage, low cost, good effect, and the like and is very convenient for the growth of the carnations.

The invention relates to antibacterial disinfection liquid soap and a preparation method, which is characterized in comprising steps as follows, (1) adding dodecyl sodium sulfate into the purified water, heating up till 70 to 80DEG C, cooling till the ambient temperature after the solution, to get liquid A; (2) dissolving povidone iodine in the liquid A, to get liquid B; (3) mixing and stirring well nonyl phenol polyethenoxy ether and dodecyl benzene sulfonic acid sodium salt, to get liquid C; (4) stirring well the liquid B and the liquid C, adding glycerin and stirring, to get the antibacterial disinfection liquid soap. The invention adopts povidone iodine which can kill the germ, the virus and the epiphyte efficiently, and combines with by the surface-active material which can remove the stains efficiently to make the liquid soap which has the effect of anti-bacterium, disinfection, decontamination, cleanliness, and no pungency. The invention is applicable to the families, the hotels, the workplaces and other places.

The invention relates to a cultivation of epiphyte that has plant infestation wireworm poisoning function and the metabolite manufacture method and the application. The strain of the epiphyte is Asipergillus. niger snf0407009 and the conserving code is CGMCC No.1714. It is made up from normal liquid fermenting and cultivating, and its metabolite has high poisoning function to plant infestation wireworm, especially root wireworm. The influence to the Meloidogyne incognita has been measured indoor. Different thickness fermenting liquid would have obviously influence to Meloidogyne incognita. One time fermenting liquid has the similar function as 10ug / mL aldicarb. It has good application prospect.

The invention relates to a method for cultivating economic fungus by adopting prickly ash seed. The method comprises extracting grease from the prickly ash seed by high-temperature squeezing to obtain prickly ash seed oil meal, mixing with wood flour and / or cotton seed hulls, sucrose, lime powder and / or gypsum powder to form economic fungus prickly ash seed-based compost with the the pH value value of 5-7.5, water content of 60-65%, and carbon-nitrogen ratio of 23-38.5, sterilizing, cooling, inoculating economic fungus strain suitable for cultivation into the compost, culturing in dark light in a cultivation chamber for 40-90 days, separating the compost from fungus fruiting body, and collecting. The method can widen application range of prickly ash seed resource, avoid resource wasting, abate environmental pressure, increase real income of farmers, effectively protect forest resource and greatly promote economic industry development of prickly ash producing area.

The invention discloses endophytic fungi from huperzia serrata. The invention is obtained through being separated from the living body of pteridophyta huperzia serrata by adopting endophytic fungi separation and purification technology, and is identified through biosystematics to be acremonium endophytium. The preservation number of the strain is CCTCC M 206118. The invention generates Huperzine A analogous compound through the strain liquid fermentation of the endophytic fungi from huperzia serrata, and is significant micro organism for searching the Huperzine A new resource of the active component for curing the presenile dementia.

The invention detects the harmful metabolite produced by epiphyte, especially aflatoxin. The outline of the invention is as follows. The filter paper, polyester cellulose mat, film of pyroxylin and filter paper are pasted on the plastic long strip bed plate. A layer of colloidal gold-monoclonal antibody is spray coated on the polyester cellulose with 50-80 OD / ml. The following two materials are spray-coated on the film of pyroxylin alternately: aflatoxin-BSA compound body and ovine antibody for anti the immunoglobulin G1 antibody of little white rat. Both of their concentration is in 1-2 mg / ml. Both of application doses is in 0.7-1.5 microlitre / cm.

The invention discloses a high throughout microbiological assay method which comprises the following steps of sampling; extracting germ plasm; mass spectrometric analysis; data analysis; and microbiological assay. The method has the advantages that thousands of kinds of bacteria, virus, epiphytes and protozoans can be identified at the same time, while other systems only can identify little infective pathogene at the same time, so that the development of the systems are limited by quantity; the method can provide the relative quantity of a plurality of pathogens in a single sample, but most systems do not have the ability currently; the method is rapid and needs not to culture, one sample can be analyzed within less than 4 hours, while a plurality of weeks are spent on culturing, certainly, some samples can not be cultured at all; environmental or clinical samples which range from air to most complex biological samples in all mediums can be analyzed, so that the method is very universal; as no sequence measurement is needed, the method is the proposal with very good cost performance for the identification of infective pathogene.

The invention discloses an armillaria luteo-virens fibrinolytic enzyme and a production method thereof. Fermentation and cultivation are made to armillaria luteo-virens ZJUQH CGMCC No.1884 to get fermented liquid and the armillaria luteo-virens fibrinolytic enzyme is separated and purified from the fermented liquid. The invention first realizes that artificial cultivated armillaria luteo-virens ZJUQH CGMCC No.1884 can be used for producing the fibrinolytic enzyme. The produced armillaria luteo-virens fibrinolytic enzyme takes agricultural and sideline products such as bean pulp, soybean flour and wheat bran with low cost as the main raw materials, thereby being safe and nonpoisonous. Being produced with a liquid fermentation method, the armillaria luteo-virens fibrinolytic enzyme is a hyper strain with relatively higher yield among the reports of epiphytes. Extraction and purification can be carried out easily and the armillaria luteo-virens is harmless, frozen-dry health products that contain thalli and enzyme liquid can be directly developed, thus providing a new thought and an evidence for developing and using novel edible fungus used in foods and medicines.

The invention is an organic waste fast-decaying bacterium agent and its constructing method, including different kinds of microbes: bacteria, actinomyces, epiphytes, etc, and different microbes have good cooperative effect. It can be obtained by directly separating and screening lignocellulose-degrading bacteria in the form of compound bacteria. It has not only high-efficacy stable degrading function to organic wastes like stalks, dejecta of domestic animals fowls, life rubbishes, etc but also strong adaptability to different conditions and antipollution ability, and has wide application prospect in regenerating and reusing organic wastes resources, environmental protection, etc.

This invention relates to a epiphyte co-culture ferment Laccase production method, which is to inoculate the bacterium detergent of the Trametes AH28-2(Trametes sp. AH28-2) in the ferment culture medium under 22-37deg C for 1-4 days, then adding the bacterium detergent of Trichoderma ZH1(Trichoderma sp. ZH1) to culture for 5-10 days, finally separating to obtain the Laccase preparation, wherein the enzyme activity is up to 6, 000U / L(guaiacol method). The culture medium is the liquid culture medium, which mainly contains a certain quantity of carbon, azotes, phosphate of kalium and natrium, sulfate of copper and ferrum, adenine and vitamin B and so on. This invention doesn't' use the aromatic compound and heavy metal iron induce, which is safe and clean, and the enzyme activity is high and stable. '

The invention publishes a kind of compound of chitosan betaine type surfactant. It contains: 0.05-1 wt% acetum, 0.025-1 wt% betaine type surfactant and the rest being water. The preparation method is: first, dissolve the chitosan in 0.1-0.2 wt% acetum to obtain 0.1-2 wt% chitosan liquid; secondly, accommodate pH to 3-6; finally, blend and insert 0.05-2 wt% betaine type surfactant and then blend sequentially for 1-3 hours to obtain the compound. The technology is secure and simple. Moreover, it produces no byproduct and answers for the request of environment-protecting. Additionally, the compound can restrain bacteria and epiphyte to grow for a long time and can be produced directly to cleaner of fruit and vegetable, sterilizing water of clothing and for various concourses, washing liquid and so forth.

The invention discloses a method for manufacturing pineapple leaf fiber antibiosis health-protection socks. The method comprises the working procedures of: spinning-dying-weaving socks, wherein the spinning process flow comprises the steps of: opening-wetting-mixing cotton-opening and cleaning cotton-carding cotton-drawing-roving-spinning. And the method is characterized in that the spinning adopts pineapple leaf fiber, cotton fiber and synthetic fiber as materials, wherein the weight percentage of the pineapple leaf fiber accounts for 10 to 60 percent and other fibers account for 40 to 90 percent. The pineapple leaf fiber contained in the materials adopted by the spinning leads the socks to be characterized by not only having soft and smooth property and comfortable feeling but also having better moisture absorbing, moisture releasing and ventilating properties and higher natural sterilization and antibiosis functions, which can effectively sterilize foot viruses, restrict the growth of epiphyte and microorganisms and largely reduce decomposition of sweat and cornifin so as to effectively prevent unpleasant smell from foot. A plurality of tests demonstrate that the pineapple leaf fiber antibiosis health-protection socks are still not smelly even without changing and washing after being worn for six or more than ten days.

The invention relates to worm grass fungus and the artificial culture method thereof, which belongs to the micro organism technology field. The worm grass fungus disclosed by the invention is high essence worm grass fungus Paecilomyces tenuipes(Peck)Samson=P. tenuipes, the artificial culture method comprises the strain activation, the mycelium culturing through liquid culture method and the fruiting body culturing with silkworm pupa. The invention adopts the main steps that the silkworm pupa is adopted as the host plant of the artificial cultured worm grass, and the fruiting body culturing with silkworm pupa is divided into two steps as follows: earlier stage (o to 5-15 days) and later stage (5 to 15 days until collection), lucifuge culturing is adopted in the earlier stage, illuminantion, abundant oxygen supply and more than 75 percent of ambient humidity are ensured in the later stage, and compared with the earlier stage, the ambient temperature of the later stage is obviously reduced; the invention can meet the scale production of the artificial worm grass.

The invention belongs to the biotechnology field, and relates to a drug potentiator biological flower foliage spraying fertilizer which is composed of materials by weight parts as follows: distilled water 9kg, brown sugar 3kg, enzymatic microorganism 40g, borax 35g, magnesium sulfate 35g, ferrous sulfate 45g, potassium dihydrogen phosphate 0.7g, manganese sulfate 35g, molybdenum trioxide 25g, zinc sulfate 35g, and copper sulfate 25g; The invention not only serves as a fertilizer, but also is a drug potentiator, and can be used together with acid pesticides with the pH value of 5.4 to 6.5. The fertilizer has adhesion after being sprayed on the foliage, thereby having good resistance to rain water, generating a protective film on the foliage, causing the flower foliage to turn green, increasing the brightness, the thickness and the width, preventing the flowers from being damaged by various epiphytes and bacteria and preventing diseases and insect pests, such as powdery mildew, acarid, red spider, etc. The drug potentiator biological flower foliage spraying fertilizer has the advantages of reliable performance, safe use, no side effects, poisonless, tasteless, no pollution and no damage to human bodies, and can be can be sprayed and flushed.

A method for artificially transplanting endophytic fungi from the natural grass to the lawn grass comprises the steps as follows: inoculating the seeds which are determined to be aseptic onto the surface of a water-agar culture medium under the aseptic condition, and slightly pressing; sealing the culture dish, placing the culture dish into a constant-temperature incubator, and carrying out the dark culture to obtain aseptic coleoptile; longitudinally cutting a small opening at the upper part of the coleoptile with a depth that the coleoptiles is not penetrated, injecting mycelia into the opening, inoculating, placing into a constant-temperature incubator and carrying out the dark culture for 5 days; culturing in the dark and in the light alternately for 4 days, transplanting into the aseptic vermiculite, sealing the flower pot opening by fresh-keeping film to increase the humidity of ambient air for one week; removing the fresh keeping film, culturing at the room temperature for one month, and detecting the infection rate. The method has a homologous transplanting success rate of 93% and a heterogenous transplanting success rate of 76%, has the advantages of rapidness, convenience, low cost, high success rate, etc., and overcomes the defect of low success rate in the prior art.

The invention relates to a leather substance and a detection method of the mould inhibition effect of a product thereof. The detection method comprises the following steps: firstly, adding NaCI solution or PBS buffer solution into a mould inclined plane culture and adjusting the concentration of spore suspension into 1*10 to 10*10 cfu / ml to prepare mould spore suspension or mixing various single mould spore suspension according to equivalent volume to prepare mixed mould spore suspension; secondly, placing the leather substance and the product thereof after being radiated by ultraviolet radiation into a culture medium and dropping the spore suspension on the leather substance to culture for 3 to 5 days at the temperature of 28 DEG C plus or minus 1 DEGC; and thirdly, carrying out visual evaluation and microscopic examination to the growth degree of epiphyte on a sample by using a microscope*50 if necessary. The detection method has the advantages of simple operation, shorter detection period and strong practicability; only mould spore is inoculated on the surface of the sample, so that the effect caused by the excessive growth of mycelium due to more mould spores is voided, the natural environment is simulated, and the growth situation of mould is analyzed.

The invention relates to a hand cream, belonging to the chemical commodity field. The hand cream is made of the plant extract and is provided with the functions of killing the epiphyte and accelerating the chap recovery on the hand skin and good market prospect.

The invention refers to a specific bacterium plant fused by the protoplast of two epiphytes and one bacterium and its structuring method which fuses the protoplast of true nucleus cell of white rotten epiphyte (phanerochaete chrysosporium), original nucleus cell (Pseudomonas) of aboriginal bacterium YZ1 and real nucleus cell of vintage yeast (Saccharomyces cerevisiae), and by three relative genes' reformation and conformity in a same cell, structures the gene-engineering specific bacterium. The technique consists of parent and three-parent transboundary fusion.

The present invention discloses an armillaria luteo-virens fibrinolytic enzyme and a production method thereof. Fermentation and cultivation are made to armillaria luteo-virens ZJUQH CGMCC No.1884 to get fermented liquid and the armillaria luteo-virens fibrinolytic enzyme is separated and purified from the fermented liquid. The armillaria luteo-virens fibrinolytic enzyme and the production method thereof of the present invention first realizes that artificial cultivated armillaria luteo-virens ZJUQH CGMCC No.1884 can be used for producing the fibrinolytic enzyme. The produced armillaria luteo-virens fibrinolytic enzyme takes agricultural and sideline products such as bean pulp, soybean flourand wheat bran with low cost as the main raw materials, thereby being safe and nonpoisonous. Being produced with a liquid fermentation method, the armillaria luteo-virens fibrinolytic enzyme is a hyper strain with relatively higher yield among the reports of epiphytes. Extraction and purification can be carried out easily and the armillaria luteo-virens is harmless, frozen-dry health products that contain thalli and enzyme liquid can be directly developed, thus providing a new thought and an evidence for developing and using novel edible fungus used in foods and medicines.

The invention discloses a construction method for a tropical rainforest hanging garden. The construction method comprises the following steps that 1, a stereoscopic simulation tree-shaped framework is built with metal pipes, a layer of bark pieces are attached to the outer surface of the framework and tied with iron wires for fixation, and a simulation landscape tree is obtained; 2, epiphytes are wound and fixed to the outer surface of the simulation landscape tree through transparent fishing lines; 3, a vessel containing liquid is arranged in the simulation landscape tree, the simulation tree-shaped framework and the vessel are connected through multiple liquid guide pipes, and then the tropical rainforest hanging garden is obtained. According to the construction method, the epiphytes can be exhibited and scientifically popularized in a warm and wet artificial environment, the simulation landscape tree can be used for a long time by serving as an exhibition carrier, an automatic water supply system can achieve automatic irrigation and feed, and a large quantity of manpower and material resources are saved.

The present invention discloses a novel anti-crop-epiphyte polypeptide and a preparation method thereof. A recombined anti-crop-epiphyte polypeptide is formed by linking gene which codes white candida pheromone and the gene which codes colicin. Compared with the prior anti-crop-epiphyte medicine, the novel anti-crop-epiphyte polypeptide of the present invention has the advantages that the novel anti-crop-epiphyte polypeptide directly forms ionic passage at epiphytic cell membrane to kill the epiphyte, so the killing efficiency is thousands times stronger than the prior anti-crop-epiphyte medicine; the epiphyte can hardly amend the geometrical damage at the cell membrane caused by the polypeptide by the mutation-expression form, so the polypeptide can not induce the epiphyte producing the traditional drug resistance; the polypeptide is a biological preparation and has target killing towards the epiphyte, so the novel anti-crop-epiphyte polypeptide does not have the toxic and side effects of the prior anti-crop-epiphyte medicine.

The invention relates to a fungi Rhizopus arrhizus and its application in Pu'er tea production, belongs to the biotechnology field.The present Rhizopus arrhizus is obtained by traditional primary screening, secondary screening, cultivation on natural medium, purification cultivation using Rhizopus arrhizus)Prhi501 as production bacterial strain, and auxiliary material.(Rhizopus arrhizus)Prhi501,The strain(Rhizopus arrhizus a) Prhi501 is preserved at CGMCC on March 1st, 2007, address being Beijing Haidian District Zhong Guan-cun northern one 13 number, preserving number being CGMCC No.1963.The inventive Rhizopus arrhizus has the effect on improving the Pu'er tea quality, and can apply to the production technology of Pu'er tea.The advantage of the invention lies in: 0.1-0.8%'s proportion of Rhizopus arrhizus is arranged in the big production process of Pu'er tea so tea polyphenols and tea brown can be controlled in appropriate proportion and reach the effect that improves the quality, and shortens process time, which stabilize and improve the pu'er tea is quality.

The invention relates to a revulsant composition for promoting the formation of mycorrhizas homobium of orchid and a preparation method thereof. The revulsant composition mainly comprises active ingredients, surface active agent and latent solvent, wherein the active ingredients are flavonoid substance and calcium salt. The invention has the advantages that the revulsant composition better exerts the function of an inoculated strain by enhancing the infestation of endogenetic epiphyte at the root of orchid or the inoculated strain, fully ensures that the orchid root and the endogenetic epiphyte establish a symbiotic relationship to form effective mycorhiza, enhances the livability of the orchid and promotes the growth of the orchid; the revulsant composition has simple use method and little dosage; and the endogenetic epiphyte at the root of the orchid or the inoculated strain can establish the symbiotic relationship with the epiphyte at the root so as to promote the formation of the mycorrhizas homobium of the orchid in such a way that only a very small quantity of the revulsant composition is needed to be added to the orchid root when irrigating the orchid from the root, thereby having important economic value and being suitable for application and popularization.

A method for predicating biomass of a submerged plant by establishing a growth simulation model comprises the following steps that firstly, a relevant dynamic mass conservation equation is obtained according to the relation among algae, organic carbon, phosphorus, nitrogen and dissolved oxygen in a lake; secondly, a relevant dynamic mass conservation equation is obtained according to the relation among the stem and root of the submerged plant and aerophyte growing around the stem; thirdly, coupling is carried out on a water quality module and a submerged plant module, and the functions of the submerged plant are added to the relevant dynamic mass conservation equations; fourthly, each model time step differential equation is solved, the biomass of the submerged plant is finally obtained, and the biomass predicating of the submerged plant is finished.

The invention discloses a epiphyte of ulmus feed addictive, its preparing process and application, wherein the product is prepared from epiphyte of ulmus solid zymogeneous bacteria and bacterium mass through solid fermentation culturing, disintegrating the culture and drying, finally packaging the obtained disintegrated articles.