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52results about How to "Easy to extract and purify" patented technology
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Yellow-green halimasch fibrinolytic enzyme and production method thereof
The invention discloses an armillaria luteo-virens fibrinolytic enzyme and a production method thereof. Fermentation and cultivation are made to armillaria luteo-virens ZJUQH CGMCC No.1884 to get fermented liquid and the armillaria luteo-virens fibrinolytic enzyme is separated and purified from the fermented liquid. The invention first realizes that artificial cultivated armillaria luteo-virens ZJUQH CGMCC No.1884 can be used for producing the fibrinolytic enzyme. The produced armillaria luteo-virens fibrinolytic enzyme takes agricultural and sideline products such as bean pulp, soybean flour and wheat bran with low cost as the main raw materials, thereby being safe and nonpoisonous. Being produced with a liquid fermentation method, the armillaria luteo-virens fibrinolytic enzyme is a hyper strain with relatively higher yield among the reports of epiphytes. Extraction and purification can be carried out easily and the armillaria luteo-virens is harmless, frozen-dry health products that contain thalli and enzyme liquid can be directly developed, thus providing a new thought and an evidence for developing and using novel edible fungus used in foods and medicines.
Owner:ZHEJIANG UNIV
Preparation method of scutellaria root flavone general aglycone extract
InactiveCN101008022AIncrease profitEasy to extract and purifyOrganic chemistryFermentationEnzymeMedicinal herbs
The invention provides a method for preparing baikal skullcap root chromocor aglycone extract, comprising following steps: disintegrating baikal skullcap root, adding water with amount being 4- 10 times of baikal skullcap root, heating to 40- 70 Deg. C and thermal insulating for 5- 24 hours, enzymolyzing, filtering after reaction, throwing away filtering liquid, extracting dregs of a decoction with ethanol, recovering ethanol, depositing, filtering and drying, getting baikal skullcap root chromocor aglycone extract. The reaction time can be adjusted along with enzymolysis temperature to get optimum enzymolysis effect and increase medicinal materials availability ratio; the water soluble foreign substance can be remove buy filtering hydrolysate, which is convenient for following chromocor aglycone extraction. The invention is characterized by high productivity, simple operation, low cost and suitability for industrial production.
Owner:SICHUAN INST OF CHINESE MATERIA MEDICA
Glucomannan enzyme preparation method
InactiveCN101182500AEfficient degradationIncrease enzyme activityFungiHydrolasesCell wallOligosaccharide
The invention relates to a preparation method of glucomannanase, which is characterized in that Trichoderma viride (Trichoderma viride) CGMCC3.2942 selected and bred by ultraviolet mutagenesis is used as an enzyme-producing strain, and the koji is cultivated in a solid koji culture medium. The solid koji medium is composed of solid medium and nutrient saline with a mass ratio of 1:2.2-2.3, wherein the solid medium is composed of bagasse 65%-73%, bran 15%-20%, yeast cell wall 10%-15% %, 2.0% to 4.0% of konjac powder, and then through the steps of enzyme liquid extraction, enzyme sludge precipitation, purification, etc., to obtain glucomannanase. The invention has the advantages of simple process, low equipment investment, low cost, no pollution, easy product extraction and purification, and the obtained glucomannanase can efficiently degrade plant-like glucomannan to obtain glucomannan oligosaccharides, and can more effectively degrade Yeast-like cell walls obtain yeast glucomannan-oligosaccharides.
Owner:GUANGDONG INST OF MICROORGANISM +1
Method for extracting syringin
InactiveCN102070684AIncrease contentLow costSugar derivativesSugar derivatives preparationChemistryActive carbon
The invention relates to a method for extracting syringin, comprising the following steps of: using Ilex rotunda as material, crushing the material to 20-80 meshes, adding 6-20 folds of water to extract for 2-3 times at 60-80 DEG C; concentrating the extraction liquid under reduced pressure, adding low-carbon alcohol to the extraction liquid for azeotropy, adding 0.5-2% of active carbon for decolorizing; adjusting the pH of decolorized liquid to 1-3, precipitating and filtering the impurity, adjusting the filtrate to be neutral by the aqueous alkali, concentrating the filtrate under reduced pressure until no alcohol exists, adding water to be saturated so as to extract n-butyl alcohol; collecting n-butyl alcohol extract liquor; recovering the n-butyl alcohol under reduced pressure, adding proper amount of water and storing the mixture to form crystal, filtering the crystal; dissolving the crystal with water and acetone solution for re-crystallization; and drying the crystal under reduced pressure to obtain the syringin product. By using the method for extracting the syringin, the one-time investment on devices for the method is less, process operation is simple and product content is high, so that the method is suitable for industrial production.
Owner:NANJING ZELANG MEDICAL TECH
Method for continuously extracting flaxseed gum and secoisolariciresinol diglucoside from flaxseed meal
InactiveCN108409813AReduce manufacturing costReduce energy consumptionSugar derivativesSugar derivatives preparationChemistryBy-product
The invention relates to a method for continuously extracting flaxseed gum and secoisolariciresinol diglucoside from flaxseed meal. The method comprises the following steps: 1) separation of shells and cores of flaxseeds: pulverizing and sieving flaxseed meal, and separately collecting upper-layer flaxseed shells and lower-layer flaxseed cores which are sieved; 2) extraction of flaxseed gum: taking the flaxseed shells as raw materials, and extracting the flaxseed gum with hot water in a microwave assisted manner; 3) extraction of secoisolariciresinol diglucoside: mixing and extracting the degummed flaxseed shells and an ethanol-sodium hydroxide solution to obtain coarse secoisolariciresinol diglucoside; and 4) purification of the secoisolariciresinol diglucoside: purifying the secoisolariciresinol diglucoside coarse product by using macroporous resin to obtain high-purity secoisolariciresinol diglucoside. The flaxseed gum and the secoisolariciresinol diglucoside are continuously extracted from the flaxseed meal, a new way for fully utilizing the flaxseed meal is provided, meanwhile, a convenient and speedy method for preparing various effective constituents of the flaxseeds is provided, flaxseed oil making by-products are utilized effectively, and the production cost of target products is reduced. The method has the advantages of low energy consumption, high product extractingrate, high economic benefit, low environment pollution and the like, and is suitable for large-scale production.
Owner:广州利众生物科技有限公司
Preparation method of graphene microsphere biological separation medium with controllable particle size
ActiveCN104759269ALarge specific surface areaImprove adsorption capacityOther chemical processesFiltration membraneMicrosphere
The invention relates to a preparation method of a graphene microsphere biological separation medium with a controllable particle size. The preparation method comprises the following steps: uniformly stirring and mixing graphene oxide and deionized water to obtain a mixed solution; performing ultrasonic treatment and cooling on the mixed solution, then atomizing into aerosol droplets, passing the aerosol droplets through a quartz tube, collecting by using a polytetrafluoroethylene (PTFE) filtration membrane, and drying to obtain graphene microspheres; and preparing a graphene microsphere separation medium taking Reactive Blue 4 as a ligand. According to the method provided by the invention, the affinity separation medium is used for separating and purifying a lot of papain from papaya powder, testing the enzymatic activity and the protein content, and calculating purification folds; and the method provided by the invention is quick, simple and convenient, is large in separation quantity, good in activity and high in purity of an extracted enzyme, and is suitable for scale production.
Owner:佛山市富馨科技实业有限公司
Double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C
ActiveCN102827895AEfficient synthesisEfficient extractionOrganic chemistryMicroorganism based processesNegative feedbackCell membrane
The invention discloses a double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C. According to the method, different surfactants are added into a liquid culture medium for changing the permeability of an antrodia camphorate bacterial cell membrane, the negative feedback inhibition effect of the target product antrondin C synthesis is reduced, on the basis, different extracting agents are coupled for carrying out in-situ extraction double-liquid-phase fermentation, and active products which are secreted from cells and attached on the bacterial ball surface are extracted into the extracting agents, so the negative feedback inhibition intensity of the target product is reduced, the surfactant coupling in-situ extraction fermentation technology is adopted, the efficient synthesis and extraction of the antrodia camphorate active product can be realized in the same fermentation period, the yield of the antrodia camphorate active product antrondin C can be obviously improved, the active product in the extraction agents is extracted into ethanol phases through ethanol extraction agents, the post-processing process is simple, the extraction and the purification of the active product are convenient, and the production cost can be effectively reduced.
Owner:JIANGNAN UNIV
Culture medium prescription for the industrial production of rhamnolipid fermentation liquor
InactiveCN101173238AIncrease productionShort cycleBacteriaMicroorganism based processesChemistryCulture mediums
The invention relates to a culture medium recipe for commercial production of rhamnolipid fermentation liquid, which is characterized in coin oil 2-5%, carbamide 0.2-1.5%, molasses 15-20%, potassium chloride 0.05-1.0%, KH2PO40.05-1.2%, K2HPO40.05-1.5%, yeast extract 0.005-0.1%, compound microelements 0.005-0.01% and the rest element is water; the pH value of the invention is 6.5-7.5. The invention has the advantages of low cost, high rhamnolipid content in fermentation, and is suitable for commercial production of rhamnolipid.
Owner:DAQING VERTEX CHEM
Genetically engineered bacterium for producing linear malt oligosaccharide generating enzyme and application of genetically engineered bacterium
ActiveCN105950528AMethod securityHigh starch conversion rate and product purityBacteriaMicroorganism based processesOligosaccharideBiotechnology
The invention discloses a genetically engineered bacterium for producing a linear malt oligosaccharide generating enzyme and application of the genetically engineered bacterium, and belongs to the technical field of microbial engineering. According to the genetically engineered bacterium, secretory expression of the linear malt oligosaccharide generating enzyme in bacillus subtilis is achieved, a method is safe and efficient, the hydrolytic activity reaches 36.6 U / mL, and the enzyme producing capacity is 10 times or above of that of an original bacterium; the obtained recombinant enzyme is an extracellular enzyme, convenience is brought for extraction and purification of large-scale production in the later period, and the product meets the food requirement. Starch is hydrolyzed by the purified recombinant linear malt oligosaccharide generating enzyme to generate linear malt oligosaccharide syrup which does not contain glucose and contains a high proportion of maltopentaose, and the linear malt oligosaccharide syrup can be directly used for producing functional food and also can be used for producing products such as high-purity linear malt oligosaccharides.
Owner:JIANGNAN UNIV
Preparation and application of nylon affinity membrane with reactive blue 4 as ligand
InactiveCN101259405AEasy to operateShort timeHydrolasesOther chemical processesBuffer solutionMethanol
The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenient and has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.
Owner:DONGHUA UNIV
Mutated NfpAB nanopore, test system, manufacturing method and application
ActiveCN111499705AImprove accuracyReduce error rateMicrobiological testing/measurementMaterial analysis by electric/magnetic meansMonomer compositionSingle strand
The invention discloses a mutant NfpAB nanopore which is a protein complex composed of at least one NfpA protein mutant monomer and at least one NfpB protein mutant monomer, or a protein complex composed of at least two mutant monomers expressed by NfpA and NfpB fusion mutant genes. The nanopore probe has a structure and recognition sites different from those of known nanopores, can be used for recognition, concentration detection and sequencing of polymers such as nucleic acid, polypeptide and protein, allows capture and transfer of single-stranded DNA and distinguishes adenine (A), thymine (T), cytosine (C) and guanine (G). The NfpAB mutant is formed by combining two different monomers, the modification space in a pore channel is enriched, the pore channel has a conical pore channel structure similar to MspA, and the NfpAB mutant is an ideal pore channel for nucleic acid sequencing. The invention also discloses a test system containing the nanopore, a manufacturing method and an application, through engineering site-specific mutagenesis in the nanopore, charge distribution in a pore channel is adjusted, the speed of nucleic acid passing through the pore channel is reduced, and accurate recognition of a basic group is realized.
Owner:SHENZHEN MERRIME NANOPORE TECH CO LTD
Effective site group of daphne giraldii nitsche leaf, preparation method, medicinal composition and application thereof
InactiveCN102302615AEnhance anti-inflammatoryHigh content of active ingredientsAntipyreticAnalgesicsDiseaseVegetation
The invention relates to an effective site group of daphne giraldii nitsche leaf, a preparation method thereof, a medicinal composition containing the effective site group of the daphne giraldii nitsche leaf and application of the effective site group of the daphne giraldii nitsche leaf to the preparation of medicaments for treating diseases such as traumatic injury, arthritis and the like, and belongs to the technical field of pharmaceutics of traditional Chinese medicine. The effective site group of the daphne giraldii nitsche leaf is high in active ingredients and obvious in medicinal effect, medicinal sites do not damage vegetations and environment, the extraction process is easy and convenient, and the operability is high.
Owner:中国人民解放军第四五一医院
Swine vesicular disease virus viral protein 1 (VP1) protein secretion expression recombinant plasmid and application thereof
InactiveCN102978227AGood for extraction and purificationImprove purification efficiencyVirus peptidesFermentationProtein proteinCell culture
The invention relates to the protein expression field and discloses swine vesicular disease virus viral protein 1 (VP1) protein secretion expression recombinant plasmid. The recombinant plasmid is composed of a gene which expresses the swine vesicular disease virus VP1 protein and a melittin signal peptide gene sequence. The recombinant plasmid has the advantages of being capable of achieving secretion and expression of the swine vesicular disease virus VP1 protein outside an insect cell, thereby directly extracting the swine vesicular disease virus VP1 protein from cell culture fluid, effectively improving protein purification efficiency, reducing preparation cost of the swine vesicular disease virus VP1 protein and laying a solid foundation for a research based on the swine vesicular disease virus VP1 protein.
Owner:SHENZHEN AUDAQUE DATA TECH
Method for dewaxing and decoloring cannabidiol extract
PendingCN111943815AGuaranteed Extraction PurityGuaranteed withdrawal rateOrganic chemistryOrganic compound preparationWaxSolvent
The invention discloses a method for dewaxing and decoloring cannabidiol extract, which is characterized by comprising the following steps: (1) pretreatment of raw materials: crushing industrial hempflower leaves, conducting baking for decarboxylation, and conducting extracting with an extracting agent at normal temperature to obtain crude extract; (2) dewaxing: dissolving the extract crude extract with ethanol to obtain an extract mixed solution, then adding a filter aid into the extract mixed solution, and carrying out freezing, cold filtration, washing, evaporation and concentration to obtain a wax-free extract crude product; and (3) decolorization: dissolving the wax-free extract crude product with ethanol to obtain a dissolved solution, adding a decolorizing agent into the dissolvedsolution, and conducting decolorizing, filtering, washing, filtering again, evaporating and concentrating to obtain golden yellow or yellowish-brown wax-free CBD extract. The method has the advantagesof simplicity in operation, lower requirement on personnel, small solvent consumption, low equipment requirement, remarkable dewaxing and decoloring effects, reasonable process, environmental protection and high efficiency.
Owner:滇麻生物科技(曲靖)有限公司
Methods for preparing roasted epimedium and medicinal liquor of roasted epimedium
InactiveCN104147072AAccelerated precipitationEasy to extract and purifyPlant ingredientsEpimediumMedicine
The invention discloses methods for preparing roasted epimedium and medicinal liquor of the epimedium. The roasted epimedium is prepared by steaming epimedium and proper auxiliary materials in a certain ratio. The medicinal liquor is prepared by crushing the prepared epimedium into coarse powder, putting the coarse powder and grain spirit in a steeping container, standing, filtering and removing residues. The prepared epimedium can be processed into medicated liquor, capsules, tables, pills, granules and oral solutions together with medicinal carriers or additives. According to the roasted epimedium prepared by the method, the icariin can be quickly dissolved, few impurities are dissolved, the property of a finished product is changed from cold to mild, the taste is changed from bitter and astringent to slightly astringent, and the color is changed from greyish-green to chartreuse; and the prepared medicinal liquor is mild in property and slightly sweet in taste, and the color of the medicinal liquor is changed from greyish-green (not prepared) to light golden brown (prepared). The quality standard of the roasted epimedium and the medicinal liquor are easy to control, the operation is simple, quick and popular, the production cost is low, and the curative effect can be easily exerted.
Owner:CHONGQING UNIV OF POSTS & TELECOMM
Method for purifying velardon with cobalt ion metal chelate affinity film
The invention relates to a method for purifying the papain by using a chelating affinity membrane of a cobalt ion metal, which comprises the following steps: (1) an activated nylon membrane is modified by the bonding of the chitosan; (2) the modified nylon membrane reacts with the mixed solution of epichlorohydrin, sodium hydroxide and sodium borohydride, then the membrane is immerged respectively into the mixed solution of sodium carbonate, sodium borohydride and coupling agent, and cobalt chloride solution to obtain the metal affinity membrane of the chelating cobalt ion; (3) the metal affinity membrane is piled up to a membrane stack, put onto a membrane bridge, transferred into Tris-HCl buffer and eluate by a peristaltic pump, then into the papain solution, is eluted by eluting agents, and then the papain is produced; and (4) the activity of enzyme and the protein content are measured, and the multiple of purification is calculated. The method has the advantages of rapidness, high efficiency, simpleness and big separation capacity. The extracted enzyme is good in activity and applicable to commercial production.
Owner:DONGHUA UNIV
Extraction method of biologically fermented carotenoid
ActiveCN111995880AAvoid the disadvantages of prolonged exposure to high temperaturesAvoid the problem of difficult recyclingNatural dyesHydrocarbonsBiotechnologyCarotenoid
The invention provides a method for extracting carotenoid from a microbial fermentation source, comprising the following steps of: (1) carrying out spray drying on a microbial fermentation solution containing carotenoid to obtain first bacterial powder; (2) carrying out wall breaking on the dry bacterial powder by using a wall breaking machine to obtain second bacterial powder; (3) carrying out saponification treatment on the wall-broken bacterial powder, and filtering to obtain third bacterial powder; (4) extracting the third bacterial powder, filtering, and collecting a filtrate; and (5) concentrating the filtrate to obtain a concentrated solution, carrying out low-temperature stirring crystallization, adding a small amount of absolute ethyl alcohol for rinsing, filtering to obtain crystals, and carrying out vacuum drying on the crystals to obtain pure carotenoid crystals. According to the method, the wall breaking rate reaches 99%, the extraction rate reaches 98% or above, the totalyield reaches 90% or above, the technological operation is simple, the conditions are mild, the carotenoid loss is small, the product quality is stable, the solvent recovery rate is high, the cost iseffectively reduced, and the method is suitable for industrial production.
Owner:HEC PHARM
Method for extracting and purifying pravastatin sodium from fermentation liquor
ActiveCN109796333AReduce typesAvoid the risk of cross-contaminationCarboxylic acid esters separation/purificationFermentationImpurity
The invention provides a method for extracting and purifying pravastatin sodium from a fermentation liquor, and belongs to the field of biological medicine preparation. According to the method for extracting and purifying pravastatin sodium from the fermentation liquor, a large amount of impurities and pigments are removed by alkalizing and acidifying the fermentation liquor, and the effective components in an acidizing liquid are enriched by an adsorbent, so that the purity of an extraction substrate is improved, the quantity of the extraction substrate is greatly reduced, and the quality ofan extraction liquid is further improved; in the whole production process, a single solvent system is adopted, the process is not complicated, the generated three wastes are low in amount, and the method is suitable for large-scale production. The content of pravastatin sodium produced by the method is more than 98.8 percent, the content of main impurity 6-epipravastatin is less than 0.15%, the content of total related substances is less than 0.4%, the product quality accords with the EP9.6 edition quality standard (the content of the pravastatin sodium is between 97.0% and 102.0%; a main impurity A, namely6-epipravastatin, is less than or equal to 0.3%; the content of the total related substances is less than or equal to 0.6%) of qualified pravastatin sodium, the total yield can reach more than 75%, and the large-scale production prospect is good.
Owner:NEW FOUNDER HLDG DEV LLC +2
Double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C
ActiveCN102827895BReduce negative feedback inhibitionEfficient synthesisOrganic chemistryMicroorganism based processesNegative feedbackCell membrane
Owner:JIANGNAN UNIV
A kind of extraction method of biological fermentation carotenoid
ActiveCN111995880BAvoid the disadvantages of prolonged exposure to high temperaturesAvoid the problem of difficult recyclingNatural dyesHydrocarbonsBiotechnologyPure crystal
The invention provides a method for extracting carotenoids derived from microbial fermentation, the method comprising: (1) spray-drying microbial fermentation liquid containing carotenoids to obtain a first bacterial powder; (2) drying the dried The bacteria powder is broken by a wall-breaking machine to obtain the second bacteria powder; (3) saponifying the broken-wall bacteria powder and filtering to obtain the third bacteria powder; (4) extracting the third bacteria powder and filtering , collecting the filtrate; (5) Concentrating the filtrate to obtain a concentrated solution, stirring and crystallizing at low temperature, adding a small amount of absolute ethanol to rinse, filtering to obtain crystals, and vacuum-drying the crystals to obtain pure carotenoid crystals. The method has a wall breaking rate of 99%, an extraction rate of over 98%, and a total yield of over 90%. The process is simple in operation, mild in conditions, less in carotenoid loss, stable in product quality, and high in solvent recovery, effectively reducing The cost is reduced, and it is suitable for industrial production.
Owner:HEC PHARM CO LTD
Extraction method of Araceae polysaccharide
InactiveCN111171170AEasy outflowEasy to extract and purifyCentrifugesCleaning using liquidsCell wallAraceae
The invention discloses an extraction method of Araceae polysaccharide, which comprises the following steps: soaking Araceae in a sodium chloride solution, carrying out ultrasonic treatment, treatingin a dialysis bag, pouring out liquid in the dialysis bag, carrying out distillation treatment, adding an ethanol solution, and carrying out repeated purification to obtain the Araceae polysaccharide.The operation method is simple, and cell walls of Araceae are broken by utilizing a sodium chloride solution, so that contents in cells are dissolved out; rapidly concentrating of the filtrate is carried out by using the dialysis bag to obtain a liquid containing less impurities; purifying is carried out for multiple times by utilizing an ethanol solution to obtain the high-purity Araceae polysaccharide.
Owner:西藏天虹科技股份有限责任公司
Escherichia coli fermentation medium and fermentation technology for producing succinic acid
InactiveCN108611377AHigh activityHigh densityMicroorganism based processesFermentationEscherichia coliDistilled water
The invention relates to an escherichia coli fermentation medium and a fermentation technology for producing succinic acid. A formula of the medium comprises 1.4-1.6g / dL corn steep liquor dry powder,0.5-2.5g / L yeast extract, 0.2-1.4g / L MgSO4, 2.5-4.5g / L (NH4)2HPO4, 0.6-2.0g / L C5H11NO2.HCl, 0.2-1.0g / L KCl, 50-60mg / L CuSO4, and 35-50mg / L FeSO4.7H2O; a pH of the medium is 6.5-6.8; and a required volume is reached by distilled water. The fermentation technology comprises the steps of taking escherichia coli as a fermentation strain, and performing aerobic enrichment in a primary seeding tank anda secondary seeding tank, and anaerobic fermentation in a fermentation tank to form succinic acid, wherein a formula of the fermentation medium is the above formula. According to the medium and the technology, the total fermentation cost can be lowered by 22%.
Owner:山东兰典生物科技股份有限公司
Culture medium for promoting synthesis of antibacterial polypeptide gramicidin and culture method thereof
PendingCN110272931AEasy to extract and purifyPromote application developmentMicroorganism based processesGramicidins S/CInhibition zoneShort stem
The invention relates to the technical field of biology, in particular to a culture medium for promoting the synthesis of antibacterial polypeptide gramicidin and a culture method thereof. The culture medium comprises the following raw materials in parts by weight: 15-25 parts of carbon source, 2-3 parts of nitrogen source, 1-3 parts of K2HPO4 and 1-3 parts of MgSO4.7H2O. In the aspect of promoting the synthesis of the linear short-stem peptide in the bacillus brevis, compared with conventional NA and NB culture mediums, the finished product of SAM liquid culture medium can greatly promote the expression of the linear gramicidin synthetase coding gene, and the expression amount is about 7-9 times of that of the conventional NB liquid culture medium, so that the synthesis amount of the linear gramicidin is greatly improved, and for specific performance, the inhibition zone on the pathogenic bacteria of Candida albicans is increased. The linear gramicidin cultured by the culture method not only can meet the research requirements, but also can promote the extraction, purification, development and application of the linear gramicidin.
Owner:GUIZHOU MEDICAL UNIV
Application of soybean RNA extract in preparation of medicine for preventing and treating enteritis
ActiveCN111249299AImprove colonic pathologyImproves inflammation levelsOrganic active ingredientsDigestive systemBiotechnologyDisease
The invention provides an application of soybean RNA extract in preparation of a medicine for preventing and treating enteritis, and belongs to the technical field of biology. In-vitro experiments show that the soybean RNA extract and gma-miR159a have no effect on proliferation activity of normal colonic epithelial cells, and have good safety; in-vivo experiments show that the soybean RNA can significantly improve the colon pathology and inflammation level of model mice with colitis, and improve the health level of the mice; therefore, the soybean RNA extract and gma-miR159a can effectively prevent and treat enteritis-related diseases. The soybean RNA extract and gma-miR159a are directly derived from soybeans, and have specific targeting, and the characteristics of safety, high efficiencyand wide sources; and the soybean RNA extract and gma-miR159a are easy to extract, have broad prospects in the fields of food and medicine, and can be used to prepare the medicine or functional foodsfor preventing and treating the enteritis.
Owner:NANJING UNIV OF FINANCE & ECONOMICS
A kind of pantophyte amidase mutant, gene, engineering bacteria and application thereof
ActiveCN107937376BIncreased parental vitalityReduce inhibitionBacteriaHydrolasesSingle mutationAmidase activity
The invention discloses a pantoea amidase mutant, a gene, an engineering bacterium and an application thereof. The amidase mutant is obtained by conducting single mutation or multiple mutation on the105th, 175th, 301st, 305th or 309th site of an amino acid sequence of pantoea amidase shown as SEQ ID No.2. The activity of the pantoea amidase mutant provided by the invention, in comparison with a parent, is improved by 2-3 times; a tolerance capacity to a substrate, namely 2-chloronicotinamide, exceeds 200mM, and the tolerance capacity can even exceed 1M by virtue of a material supplementing method; and a conversion rate can be still kept above 95%. With the application of the material supplementing method provided by the invention, an inhibitory effect of 2-chloronicotinic acid to an amidase product or bacterium product can be effectively relieved, catalysis efficiency can be kept at relatively high level, and meanwhile, the separation-out of the finished product, namely the 2-chloronicotinic acid, can be promoted to the greatest extent, so that the extraction and purification of the finished product are facilitated. Therefore, the amidase mutant provided by the invention is applicable to the enzymatic industrial production of the 2-chloronicotinic acid.
Owner:ZHEJIANG UNIV OF TECH
Process for purifying bromelain by chelated metal affinity membrane
The invention relates to a method for purifying bromelain by means of a metal chelate affinity membrane. The method comprises the following steps that: (1) activated nylon membrane is modified through hydroxyethyl cellulose bonding; (2) the modified nylon membrane reacts with the mixed solution of epichlorohydrin, sodium hydroxide and sodium borohydride, and then the membrane is respectively dipped in the mixed solution of sodium carbonate, sodium borohydride and a coupling agent and a zinc sulfate solution, thereby generating a chelated zinc ionic metal affinity membrane; (3) the metal affinity membrane is piled up into a membrane stack, and is fed into a membrane bridge; a peristaltic pump pumps in a Tris-HCl buffer solution and an eluent and then pumps in a bromelain solution so as tocarry out elution by eluant, thereby generating bromelain; (4) enzymatic activity and protein content are tested to calculate purification multiple. The method has quickness, high efficiency, simplicity, convenience, great fractional dose and high activity of extracted enzyme, and is suitable for industrialscale production.
Owner:DONGHUA UNIV
A kind of extraction method and application of tamarind polysaccharide
ActiveCN112794926BFully dissolvedPromote denaturationChemical industryUltrafiltrationEthanol precipitation
The invention provides a method for extracting tamarind polysaccharides and its application. The extraction method comprises: pretreating tamarind seeds to obtain tamarind rubber powder, adding trichloroacetic acid aqueous solution, stirring and reacting at 100-150°C, The tamarind polysaccharide mixture is obtained, the filtrate is taken after solid-liquid separation, concentrated, alcohol extracted and dried to obtain the obtained product. The extraction method of the present invention utilizes the high-temperature-resistant and difficult-to-degrade properties of tamarind polysaccharides. On the basis of pretreatment, pressurized water extraction is performed under ultra-high temperature acidic conditions to ensure that tamarind polysaccharides can be fully dissolved, and to promote protein extraction at ultra-high temperatures and trichloroacetic acid. Denaturation and precipitation under double conditions; ultrafiltration and concentration are used to further remove small molecular impurities such as some polypeptide proteins, amino acids and inorganic salts degraded by high temperature, and combined with ethanol precipitation to separate and obtain high-purity tamarind polysaccharides; high extraction efficiency, easy to scale up, suitable for batches Chemical production, the prepared tamarind polysaccharide protein content is lower than 0.3wt%.
Owner:WUHAN BIBIWEISHA BIOLOGICAL TECH
A kind of preparation method of pentafluorophenol
ActiveCN106810423BMild reaction conditionsIncreased process safetyOrganic compound preparationGroup 3/13 element organic compoundsOrganic synthesisBiochemical engineering
Owner:上海万溯药业有限公司 +1
Application of orientin in the preparation of drugs for inhibiting angiogenesis
ActiveCN104000812BSuppress generationLow cytotoxicityOrganic active ingredientsAntineoplastic agentsOrientinCancer drugs
The invention discloses the application of a compound orientin in the preparation of an angiogenesis-inhibiting drug. The angiogenesis-inhibiting drug comprises orientin and a pharmaceutically acceptable carrier. The orientin provided by the invention has a strong inhibitory effect on angiogenesis when applied to an angiogenesis inhibitor, is a novel and effective method for treating malignant tumors, and has low cytotoxicity, and is an efficient and low-toxicity inhibiting angiogenesis and then treating The drug resource of cancer is a monomer compound with natural origin, which exists in a variety of Chinese herbal medicines. It is very convenient to extract and purify, and it can also be synthesized.
Owner:SOUTHEAST UNIV
Method for preparing olivine type five-element high-entropy lithium battery precursor by recycling lithium battery
ActiveCN112093787ASimple recycling processReduce recycling costsWaste accumulators reclaimingPhosphorus compoundsLithium iron phosphatePhysical chemistry
The invention belongs to the field of lithium battery recycling, and particularly relates to a method for preparing an olivine-type five-element high-entropy lithium battery precursor material by recycling retired lithium batteries. The method comprises the following steps: mixing and roasting retired nickel-cobalt-manganese ternary and lithium iron pole pieces in an air atmosphere; introducing air into the obtained pole piece in dilute acid for reaction to obtain first filtrate and filter residues; adding phosphoric acid and a hydrogen peroxide solution into the filter residues for dissolving, adjusting the pH of the solution after adjusting the concentration of metal ions in the solution, and reacting to generate an olivine type five-element high-entropy lithium battery material precursor; and washing, filtering, washing, drying and roasting the obtained precursor slurry to obtain a five-element high-entropy lithium battery material precursor. According to the method, the retired nickel-cobalt-manganese ternary and lithium iron phosphate positive plates are recycled in a unified manner, so that the retired lithium battery material recycling process is greatly simplified, and theprepared olivine type five-element high-entropy lithium battery precursor material is relatively high in capacity, stable in high-temperature performance and relatively slow in capacity attenuation.
Owner:SINOSTEEL NANJING NEW MATERIALS RES INST CO LTD +1
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