52results about How to "Easy to extract and purify" patented technology

The invention discloses a double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C. According to the method, different surfactants are added into a liquid culture medium for changing the permeability of an antrodia camphorate bacterial cell membrane, the negative feedback inhibition effect of the target product antrondin C synthesis is reduced, on the basis, different extracting agents are coupled for carrying out in-situ extraction double-liquid-phase fermentation, and active products which are secreted from cells and attached on the bacterial ball surface are extracted into the extracting agents, so the negative feedback inhibition intensity of the target product is reduced, the surfactant coupling in-situ extraction fermentation technology is adopted, the efficient synthesis and extraction of the antrodia camphorate active product can be realized in the same fermentation period, the yield of the antrodia camphorate active product antrondin C can be obviously improved, the active product in the extraction agents is extracted into ethanol phases through ethanol extraction agents, the post-processing process is simple, the extraction and the purification of the active product are convenient, and the production cost can be effectively reduced.

The invention provides a method for extracting carotenoids derived from microbial fermentation, the method comprising: (1) spray-drying microbial fermentation liquid containing carotenoids to obtain a first bacterial powder; (2) drying the dried The bacteria powder is broken by a wall-breaking machine to obtain the second bacteria powder; (3) saponifying the broken-wall bacteria powder and filtering to obtain the third bacteria powder; (4) extracting the third bacteria powder and filtering , collecting the filtrate; (5) Concentrating the filtrate to obtain a concentrated solution, stirring and crystallizing at low temperature, adding a small amount of absolute ethanol to rinse, filtering to obtain crystals, and vacuum-drying the crystals to obtain pure carotenoid crystals. The method has a wall breaking rate of 99%, an extraction rate of over 98%, and a total yield of over 90%. The process is simple in operation, mild in conditions, less in carotenoid loss, stable in product quality, and high in solvent recovery, effectively reducing The cost is reduced, and it is suitable for industrial production.

The invention discloses a pantoea amidase mutant, a gene, an engineering bacterium and an application thereof. The amidase mutant is obtained by conducting single mutation or multiple mutation on the105th, 175th, 301st, 305th or 309th site of an amino acid sequence of pantoea amidase shown as SEQ ID No.2. The activity of the pantoea amidase mutant provided by the invention, in comparison with a parent, is improved by 2-3 times; a tolerance capacity to a substrate, namely 2-chloronicotinamide, exceeds 200mM, and the tolerance capacity can even exceed 1M by virtue of a material supplementing method; and a conversion rate can be still kept above 95%. With the application of the material supplementing method provided by the invention, an inhibitory effect of 2-chloronicotinic acid to an amidase product or bacterium product can be effectively relieved, catalysis efficiency can be kept at relatively high level, and meanwhile, the separation-out of the finished product, namely the 2-chloronicotinic acid, can be promoted to the greatest extent, so that the extraction and purification of the finished product are facilitated. Therefore, the amidase mutant provided by the invention is applicable to the enzymatic industrial production of the 2-chloronicotinic acid.

The invention relates to a method for purifying bromelain by means of a metal chelate affinity membrane. The method comprises the following steps that: (1) activated nylon membrane is modified through hydroxyethyl cellulose bonding; (2) the modified nylon membrane reacts with the mixed solution of epichlorohydrin, sodium hydroxide and sodium borohydride, and then the membrane is respectively dipped in the mixed solution of sodium carbonate, sodium borohydride and a coupling agent and a zinc sulfate solution, thereby generating a chelated zinc ionic metal affinity membrane; (3) the metal affinity membrane is piled up into a membrane stack, and is fed into a membrane bridge; a peristaltic pump pumps in a Tris-HCl buffer solution and an eluent and then pumps in a bromelain solution so as tocarry out elution by eluant, thereby generating bromelain; (4) enzymatic activity and protein content are tested to calculate purification multiple. The method has quickness, high efficiency, simplicity, convenience, great fractional dose and high activity of extracted enzyme, and is suitable for industrialscale production.

The invention relates to the field of organic synthesis and in particular relates to a preparation method of pentafluorophenol. The structural formula of pentafluorophenol is as shown in a formula I. The preparation method specifically comprises the following steps of: (1) decarboxylic reaction; (2) Grignard reaction; and (3) oxidizing reaction. According to the preparation method of pentafluorophenol, raw materials are easily available and are low in cost, the production cost is low, the waste of bromine resources is avoided, and adverse effects on the environment are reduced; meanwhile, the preparation method of pentafluorophenol is mild in reaction condition, high in process safety, few in product impurity, easy in extraction and purification and stable in quality; and the content of pentafluorophenol obtained by refining is greater than 99%, so that the preparation method is completely suitable for large-scale industrial production.