Double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C

Abstract

The invention discloses a double-liquid-phase fermentation method of coupling in-situ extraction antrodia camphorate active product antrondin C. According to the method, different surfactants are added into a liquid culture medium for changing the permeability of an antrodia camphorate bacterial cell membrane, the negative feedback inhibition effect of the target product antrondin C synthesis is reduced, on the basis, different extracting agents are coupled for carrying out in-situ extraction double-liquid-phase fermentation, and active products which are secreted from cells and attached on the bacterial ball surface are extracted into the extracting agents, so the negative feedback inhibition intensity of the target product is reduced, the surfactant coupling in-situ extraction fermentation technology is adopted, the efficient synthesis and extraction of the antrodia camphorate active product can be realized in the same fermentation period, the yield of the antrodia camphorate active product antrondin C can be obviously improved, the active product in the extraction agents is extracted into ethanol phases through ethanol extraction agents, the post-processing process is simple, the extraction and the purification of the active product are convenient, and the production cost can be effectively reduced.

Description

technical field [0001] The invention relates to a fermentation method of Antrondin C, an active product of Antrodia camphorata, in particular combining liquid fermentation for regulating cell permeability with in-situ extraction to establish a suitable two-liquid phase coupling extraction fermentation system, which belongs to the field of fermentation engineering. technical background [0002] Antrodia camphorata (Antrodia camphorata), also known as Antrodia camphorata and Red Antrodia camphorata, is an extremely precious edible and medicinal mushroom in the field of traditional Chinese medicine. camphor tree aroma. [0003] In the past few years, due to the wide range of pharmacological activities and low toxicity of Antrodia camphorata, it has gradually attracted international attention, and the natural products with medicinal effects extracted from it have also been valued by various scholars and industries. Many research institutes have devoted manpower and material res...

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problems of low output and few types of physiologically active products synthesized by conventional liquid fermentation of Antrodia camphorata, and provides a two-liquid phase coupling fermentation method for producing active products of Antrodia camphorata by coupling in-situ extraction and fermentation with surfactants. The active agent changes the permeability of the cell membrane, reduces the negative feedback inhibition effect of the physiologically active products of Antrodia camphorata, and can effectively increase the types and yields of physiologically active products of Antrodia camphorata. However, during the fermentation process, these products are difficult to dissolve in water, so The active product secreted from the cell and still attached to the surface of the fungus ball can be enriched into the extractant phase by extractant extraction, so as to realize the efficient synthesis and extraction of the active product of Antrodia camphorata in the same fermentation cycle. The active product in the extractant phase passes through Ethanol is extracted into the ethanol phase, which makes the extraction and purification of the physiologically active products of Antrodia camphorata easy, and the recovered extractant can be reused after distillation to remove residual ethanol, thereby reducing production costs