A kind of extraction method and application of tamarind polysaccharide

Abstract

The invention provides a method for extracting tamarind polysaccharides and its application. The extraction method comprises: pretreating tamarind seeds to obtain tamarind rubber powder, adding trichloroacetic acid aqueous solution, stirring and reacting at 100-150°C, The tamarind polysaccharide mixture is obtained, the filtrate is taken after solid-liquid separation, concentrated, alcohol extracted and dried to obtain the obtained product. The extraction method of the present invention utilizes the high-temperature-resistant and difficult-to-degrade properties of tamarind polysaccharides. On the basis of pretreatment, pressurized water extraction is performed under ultra-high temperature acidic conditions to ensure that tamarind polysaccharides can be fully dissolved, and to promote protein extraction at ultra-high temperatures and trichloroacetic acid. Denaturation and precipitation under double conditions; ultrafiltration and concentration are used to further remove small molecular impurities such as some polypeptide proteins, amino acids and inorganic salts degraded by high temperature, and combined with ethanol precipitation to separate and obtain high-purity tamarind polysaccharides; high extraction efficiency, easy to scale up, suitable for batches Chemical production, the prepared tamarind polysaccharide protein content is lower than 0.3wt%.

Description

A kind of extraction method and application of tamarind polysaccharide technical field The present invention relates to the extraction technical field of plant polysaccharide, more specifically, relate to a kind of extraction method of tamarind polysaccharide law and its applications. Background technique Tamarind is a kind of large-scale tree of the genus Hematoxylinaceae, also known as sour bean, sour bean; its seed is approximate rectangle or egg Round shape, hard skin, reddish-brown, shiny, containing 60-73wt% of non-fibrous polysaccharides and 15- of protein 20wt%, fat and other impurities 8-13wt%. In the 1960s, Japan took the lead in realizing the industrialized production of tamarind polysaccharide, and was sold as a food additive. After that, the United States, Britain, France, China and other countries have also started commercial production of tamarind polysaccharide. It is widely used in weaving, latex, construction, wood, adhesives, explosives, paperm...

AI Technical Summary

Problems solved by technology

However, the development and application of tamarind polysaccharides in China started relatively late, and there are few related research reports. It is only widely used in food production, and its deep-processed high value-added products are extremely scarce. High-purity tamarind polysaccharides There is no relevant domestic research report on the mass production preparation technology

[0005] The Chinese invention patent application with the publication number CN1034278A discloses a preparation method of tamarind gum, which is prepared by heating tamarind seed kernels, cooling them, grinding and decolorizing them, soaking in unleaded gasoline for degreasing, crushing, and sieving; the method The preparation process is simple and easy, the cost is low, and it can meet the requirements of tobacco sheet adhesives, but the purity of tamarind polysaccharide is not high, and the application range is narrow

[0006] The Chinese invention patent application with the publication number CN107501424A discloses a method for purifying tamarind polysaccharide gum, which mainly adopts the method of dispersing the calendered and broken tamarind polysaccharide gum in low-concentration ethanol-water solution to remove tamarind polysaccharide gum through enzymatic degradation. The purification method of protein in Wangzi polysaccharide gum improves product quality; this method can effectively avoid the problem of high viscosity of polysaccharide gum aqueous solution system, and can significantly reduce the protein content in the product, but the protein content in the product obtained by its best embodiment The impurity content is as high as 6-8wt%, that is, the product purity still cannot meet the needs of practical application

[0007] Publication number is that the Chinese invention patent application of CN107892722A discloses a kind of preparation method of water-soluble tamarind polysaccharide, adopts citric acid solution to carry out extraction alcohol precipitation, obtains the white powder product that protein content is lower than 3wt%; Although the extraction rate of this method is higher High, but repeated alcohol precipitation is required, the operation steps are complicated, and the consumption of ethanol is large, which is not conducive to industrial production

[0008] The Chinese invention patent application with the publication number CN106496350A discloses a method for preparing tamarind gum with high color yield, through carboxymethyl modification, microbial modification and grafting of hydrophilic groups to tamarind gum to solve the problem of tamarind offset printing. The problem of poor color yield of flowers, but the products of this solution are mainly used in the field of fabric printing technology