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44results about How to "High purification factor" patented technology
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Nylon affinity membrane preparing method and use
InactiveCN1935339AHigh purification factorSmall pressure dropSemi-permeable membranesHydrolasesCibacron Blue F3GABiological macromolecule
The present invention discloses a preparation method of nylon affinity membrane. Said method includes the following steps:(1) on the nylon membrane chemically-bonding chitosan so as to obtain the chitosan modified nylon membrane; and (2) bonding the chitosan modified nylon membrane and affinity dye Cibacron Blue F3GA so as to obtain the described nylon affinity membrane which can be used for separating and purifying papain.
Owner:DONGHUA UNIV
Method for coextracting uranium, plutonium and neptunium
ActiveCN102776372AHigh yieldLow extraction rateNuclear energy generationRecycling and recovery technologiesAfter treatmentPu element
The invention belongs to the technical field of nuclear fuel cycles, and discloses a method for coextracting uranium, plutonium and neptunium. The method is implemented in a way that: neptunium is coextracted with uranium and plutonium at the co-decontamination section, so that the neptunium enters an organic phase 1AP. The key point is to oxidate Np (V) in an extraction apparatus 1A into Np (VI) which can be extracted by the extraction apparatus. The method implements high yields of uraniumm, plutonium and neptunium, does not increase the salt content in the after-treatment process, and can implement quantitative extraction of the uranium, plutonium and neptunium.
Owner:CHINA INSTITUTE OF ATOMIC ENERGY
Method for purifying alliinase by double-water-phase separation
The invention belongs to the field of deep processing of garlic, and particularly discloses a method for purifying alliinase by double-water-phase separation. The method comprises the following steps: (1) peeling and precooling garlic squamous bulbs, immersing in a precooled extract, homogenizing, centrifuging, and discarding garlic slag, thereby obtaining the supernate crude enzyme solution; (2) dissolving ammonium sulfate, sodium dihydrogen phosphate or trisodium citrate in a Britton-Robinson buffer solution, oscillating to completely dissolve, adding a PEG (polyethylene glycol) solution, evenly mixing by oscillation, and standing for phase splitting, thereby obtaining a double-water-phase extractant; (3) adding the crude enzyme solution into the double-water-phase extractant, oscillating, centrifuging, and standing for phase splitting; and (4) ultrafiltering the lower phase of the enriched alliinase, and carrying out vacuum freeze drying on the trapped fluid to obtain the alliinase solid. The method is simple to operate, has the advantages of simple apparatuses, time saving, low cost, and higher alliinase purification multiple and enzyme activity recovery rate than the prior art, and can easily implement scale-up production.
Owner:QILU UNIV OF TECH
Preparation method of graphene microsphere biological separation medium with controllable particle size
ActiveCN104759269ALarge specific surface areaImprove adsorption capacityOther chemical processesFiltration membraneMicrosphere
The invention relates to a preparation method of a graphene microsphere biological separation medium with a controllable particle size. The preparation method comprises the following steps: uniformly stirring and mixing graphene oxide and deionized water to obtain a mixed solution; performing ultrasonic treatment and cooling on the mixed solution, then atomizing into aerosol droplets, passing the aerosol droplets through a quartz tube, collecting by using a polytetrafluoroethylene (PTFE) filtration membrane, and drying to obtain graphene microspheres; and preparing a graphene microsphere separation medium taking Reactive Blue 4 as a ligand. According to the method provided by the invention, the affinity separation medium is used for separating and purifying a lot of papain from papaya powder, testing the enzymatic activity and the protein content, and calculating purification folds; and the method provided by the invention is quick, simple and convenient, is large in separation quantity, good in activity and high in purity of an extracted enzyme, and is suitable for scale production.
Owner:佛山市富馨科技实业有限公司
Method for separating and purifying protease capable of degrading soybean protein allergens
InactiveCN104531644AIncreased specific enzyme activityGood inhibitory effectHydrolasesVegetable proteins working-upHeterologousFiltration
The invention discloses a method for separating and purifying a protease capable of degrading soybean protein allergens, belonging to the technical field of protein purification. The method comprises the following step: separating and purifying the protease which is sourced from a wild strain Bacillus sp.BBE201108 and has the effect of degrading soybean protein allergens by sequentially adopting ammonium sulfate distributed precipitation, dialysis, anion exchange chromatography (Q FF) and gel filtration chromatography (Superdex 75). The protease disclosed by the invention can be successfully prepared by virtue of separation and purification, and a foundation for analyzing enzymatic properties and amino acid sequences of the protease and achieving heterologous expression research of the protease can be provided.
Owner:JIANGNAN UNIV
Method for extracting lactoperoxidase by ultrafiltration assisted two aqueous phase extraction technology
ActiveCN104450639AHigh recovery rateHigh purification factorOxidoreductasesFood additiveUltrafiltration
The invention discloses a method for extracting lactoperoxidase by the ultrafiltration assisted two aqueous phase extraction technology. The method comprises the steps of using bovine whey as the raw material; enriching all lactoperoxidase from bovine whey into a salt phase by the two aqueous phase extraction technology; processing the salt phase solution through an ultrafiltration membrane to remove small impure protein; further purifying lactoperoxidase; desalting through a dialysis bag; then freezing and drying, so as to prepare a high-activity lactoperoxidase product which can be used in natural food additives. With the adoption of the method, lactoperoxidase with natural antibacterial activity can be directly prepared and directly added as a preservative into foods or toothpaste and other daily supplies.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Preparation and application of nylon affinity membrane with reactive blue 4 as ligand
InactiveCN101259405AEasy to operateShort timeHydrolasesOther chemical processesBuffer solutionMethanol
The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenient and has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.
Owner:DONGHUA UNIV
Modified beer waste yeast adsorbent, preparation method and application thereof
InactiveCN102228823AImprove adsorption capacityReduce dosageOther chemical processesEnzymesCross-linkCitric acid
The invention relates to the technical field of biosorbent preparation and application, and specifically discloses a modified beer waste yeast adsorbent, a preparation method and an application thereof. The modified beer waste yeast adsorbent provided by the present invention is a novel adsorbent which is prepared from a cross-linked beer waste yeast modified through citric acid, and provides good absorbability for lysozyme such that the modified beer waste yeast adsorbent can be applicable for extracting the lysozyme.
Owner:SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
Method for purifying velardon with cobalt ion metal chelate affinity film
The invention relates to a method for purifying the papain by using a chelating affinity membrane of a cobalt ion metal, which comprises the following steps: (1) an activated nylon membrane is modified by the bonding of the chitosan; (2) the modified nylon membrane reacts with the mixed solution of epichlorohydrin, sodium hydroxide and sodium borohydride, then the membrane is immerged respectively into the mixed solution of sodium carbonate, sodium borohydride and coupling agent, and cobalt chloride solution to obtain the metal affinity membrane of the chelating cobalt ion; (3) the metal affinity membrane is piled up to a membrane stack, put onto a membrane bridge, transferred into Tris-HCl buffer and eluate by a peristaltic pump, then into the papain solution, is eluted by eluting agents, and then the papain is produced; and (4) the activity of enzyme and the protein content are measured, and the multiple of purification is calculated. The method has the advantages of rapidness, high efficiency, simpleness and big separation capacity. The extracted enzyme is good in activity and applicable to commercial production.
Owner:DONGHUA UNIV
Tyrosinase inhibitor and preparation method thereof
InactiveCN102336809AEnhanced inhibitory effectLow cytotoxicityMicrobiological testing/measurementPeptide preparation methodsCytotoxicityBiocompatibility Testing
The invention relates to a tyrosinase inhibitor and a preparation method thereof. The sequence of the tyrosinase inhibitor is Ile-Gln-Ser-Pro-His-Phe-Phe. The preparation method comprises the following steps: (1) with tyrosinase molecules as targeting molecules, screening considerable bacteriophages having different affinity for the tyrosinase molecules; (2) amplifying screened bacteriophages with affinity for subsequent biospanning; (3) determining affinity of a plurality of screened monoclonal bacteriophages and selecting monoclonal bacteriophages with strongest bonding force for DNA sequencing and synthesis of specific ligands. The tyrosinase inhibitor provided in the invention has a good inhibitory effect; the tyrosinase inhibitor is an endogenous substance of human bodies and has good biocompatibility and low cytotoxicity, and therefore, the tyrosinase inhibitor is more suitable for being applied in fields of whitening cosmetics, preservation of fruit and vegetable, biopesticides, etc. The preparation method has the advantages of simple operation and little time consumption; the prepared tyrosinase inhibitor has a high purification fold and high enzyme activity.
Owner:DONGHUA UNIV
Purification method of acetylcholinesterase from earthworm serum
ActiveCN102660516ASources are cheap and wideHigh sensitivityHydrolasesWater bathsPurification methods
The invention provides a purification method of acetylcholinesterase from earthworm serum. The method comprises the following steps: placing earthworm in clean water for 24 hours to eliminate excrement in the earthworm body, and storing the earthworm in a refrigerator for later use; homogenizing with phosphoric acid buffer solution in an ice-water bath condition to prepare homogenate, centrifuging to obtain crude enzyme extract, and storing in the refrigerator; adding surfactant Triton X-114 solution and citric acid buffer solution into 100mu L of crude extract, and diluting with secondary water until the volume is 10mL, sufficiently oscillating and shaking uniformly; placing the solution in a constant-temperature water bath with the water bath temperature of 35 DEG C to balance for 9 minutes; and centrifuging for layering to obtain a lower product which is the purified acetylcholinesterase product. The purification extraction method of the acetylcholinesterase from earthworm serum has wide enzyme sources and low price, high sensitivity, simple and stable preparation process and low production cost.
Owner:武汉中科志康生物科技有限公司
Antifungal protein, method for preparing same and application thereof
InactiveCN101591385AHigh purification factorReduced purification stepsBiocideFood preservationChromatographic separationIon exchange
The invention provides an antifungal protein, which is obtained by purifying a water soluble extract of hulless oat seed, wherein the apparent molecular weight of the antifungal protein is about 35kDa. The preparation method comprises the following steps: crushing and degreasing the hulless oat seed; extracting the hulless oat seed by buffer solution to obtain a coarse protein; and obtaining the antifungal protein by the two-step chromatographic separation which is the Resource S cation exchange and Superdex 75 gel exclusion chromatographic purification. The antifungal protein has obvious effect of inhibiting the growth of white rot fungus and trichoderma viride, and the minimum inhibition for the white rot fungus is 0.42mu g, and 0.21mu g for the thetrichoderma viride. In addition, the antifungal protein also has certain effect of inhibiting the growth of fusarium. The antifungal protein can be used as a food anticorrosion additive, a crop antifungal damage spraying agent and the like, and has high practical values in the aspects of food anticorrosion, crop prophylaxis and crop disease resistance.
Owner:SHANXI UNIV
Intermittent operation type high-level liquid waste evaporation-denitration equipment
InactiveCN108447578AEasy to carryHigh purification factorRadioactive decontaminationLiquid wasteEvaporation
The invention provides intermittent operation type high-level liquid waste evaporation-denitration equipment. The equipment comprises an evaporation kettle and a purification column, wherein the evaporation kettle comprises a kettle body, and a heating jacket outside the kettle body; the kettle body is provided with a material inlet and outlet and a steam outlet; the heating jacket is provided with a heating medium inlet and a heating medium outlet; the purification column comprises a column body, and a cooling jacket outside the column body; a steam inlet is arranged at the bottom of the purification column, and an exhaust gas outlet and a water inlet pipe are arranged at the top of the purification column; a spray head is connected to an opening end of the water inlet pipe inside the purification column; a lower orifice plate and an upper orifice plate are arranged in the purification column, and a filler is filled between the lower orifice plate and the upper orifice plate; and thecooling jacket is provided with a cooling medium inlet and a cooling medium outlet.
Owner:CHINA INSTITUTE OF ATOMIC ENERGY
A kind of preparation method of antitussive, expectorant, anti-inflammatory, anti-microbial extract from earthworms
ActiveCN109260231BEasy to prepareShort manufacturing timeAntibacterial agentsAntipyreticBiotechnologyMicroorganism
The invention provides a preparation method of an earthworm extract for relieving cough, eliminating phlegm, anti-inflammation and anti-microbial. The extraction method provided by the invention is mainly to extract the earthworm homogenate through NaAC buffer solution of pH 4.8-5.4 and acetone. After repeated tests, the present invention optimizes the extraction process provided by the applicant of the present invention, which greatly shortens the preparation time of the earthworm extract from 42-45 hours to 15-18 hours; and the yield increases from 73% to 100%. 82%, the recovery rate of the total activity has been improved, the specific activity has been increased from 1141 to 4443, and the specific activity has been increased by 3.9 times; the purification factor has increased from 41 times to 158 times, and the purification factor of the deoxyribonuclease active ingredient has increased by nearly four times. The preparation method provided by the invention has simple process, low cost and is easy to be popularized and applied in industrialized production. The purity and activity of the product prepared by the preparation method provided by the invention are significantly higher than that of related similar products prepared by the existing method.
Owner:首都儿科研究所
Process for purifying zirconium in Purex flow plutonium purification cycle
The invention belongs to the technical field of spent fuel post-treatment, and discloses a process for purifying zirconium in a Purex flow plutonium purification cycle. 30% tri-butyl-phosphate (TBP)-kerosene is adopted as an extraction agent 2AX in a 2A extractor, so as to recover uranium and plutonium and purify fission fragments. The process comprises an extraction section and a washing section; and the concentration of a nitric acid in a 2AF feed liquid is 3.5-3.7mol / L, and 2AS is an HNO3 solution of which the concentration is 0.8-0.85mol / L. The process has the advantages that the plutonium yield is greater than 99.9%, and the purification coefficient of zirconium is greater than 100.
Owner:CHINA INSTITUTE OF ATOMIC ENERGY
Method for purifying hyaluronidase by affinity membrane chromatography
The invention provides a method for purifying a hyaluronidase by an affinity membrane chromatography, which uses a nylon membrane as a matrix, a method of bonding chitosan is used to modify the nylonmembrane, so that the non-specific adsorption of the membrane is greatly reduced, and a novel dye affinity membrane is obtained, which has good adsorption to hyaluronidase and has a high purificationratio.
Owner:青岛冠龙生物制药有限公司
Purification method of natural thrombin regulatory protein
PendingCN109354621AHigh purification factorHigh purityPeptide preparation methodsAnimals/human peptidesProtein solutionPurification methods
The invention discloses a purification method of natural thrombin regulatory protein. The method includes the following steps: (1) selecting a composite chromatography column with hydrophobic groups and anisole as a ligand, and then preparing a balancing solution and an eluent; balancing the composite chromatography column by phosphate with 3-5 times of column volume until the conductivity and pHvalue are consistent with that of the balancing solution; (2) taking a thrombin regulating protein solution intermediate with a concentration of 0.005-10 mg / mL, adjusting the pH value of the thrombinregulating protein solution intermediate to 4.0-8.0, sampling and performing chromatography purification on the composite chromatography column, using the balancing solution with a pH value of 4.0-8.0for flushing after the sampling is completed, then using a flushing liquid with a pH value of 4.0-8.0 for flushing, eluting with eluent, and collecting the eluent; (3) ultrafiltering the eluent obtained in the step (2) to obtain a concentrated solution; (4) pouring the concentrated solution obtained in the step (3) to a freeze-drying plate for freeze-drying to obtain a thrombin regulatory proteinfinished product. The method is simple in operation, high in yield, and high in purity.
Owner:YANGZHOU AIDEA BIOTECH
Preparation method of antitussive, expectorant, anti-inflammatory, and antimicrobial extract from earthworm
ActiveCN109260231AEasy to prepareShort manufacturing timeAntibacterial agentsAntipyreticAntibiotic resistanceAnti-inflammatory analgesics
The invention provides a preparation method of an antitussive, expectorant, anti-inflammatory and anti-microbial extract from earthworms. The extraction method provided by the invention mainly extracts the earthwormhomogenate through NaAC buffer and acetone of pH 4.8-5.4. The invention optimizes the extraction process provided by the applicant through repeated experiments, greatly shortens the preparation time of earthworm extract, and reduces the preparation time of earthworm extract from 42to 45 hours to 15 to18 hours; the yield was increased from 73% to 82%, and the recovery of total activity was increased from 1141 to 4443, and the specific activity was increased by 3.9 times. The purification times increased from 41 times to 158 times, and the purification times of the active component of deoxyribonuclease increased nearly fourfold. The preparation method provided by the invention is simple in process, low in cost and easy to be popularized and applied in industrial production. The purity and the activity of the product prepared by the preparation method provided by the invention are significantly higher than those of the related similar products prepared by the prior art.
Owner:首都儿科研究所
Recombinant beta-galactosidase and construction method and application thereof
ActiveCN111592600AFast self-purificationSimple self purificationBacteriaAntibody mimetics/scaffoldsEngineeringBioengineerings
The invention provides recombinant beta-galactosidase and a construction method and application thereof, and belongs to the technical field of bioengineering. The recombinant beta-galactosidase beta-Gal-LEH is constructed in the invention, and beta-Gal and ELP (VPGVG) 50 are connected to construct the recombinant beta-galactosidase beta-Gal-LEH which can be self-purified and has good stability andhigh lactose hydrolysis efficiency.
Owner:JIANGSU UNIV
Method for extracting lipoxygenase from soybean whey waste water through inverse pH gradient
ActiveCN109852591AIncrease enzyme activityHigh recovery rate of enzyme activityOxidoreductasesSocial benefitsUltrafiltration
The invention discloses a method for extracting lipoxygenase from soybean whey waste water through inverse pH gradient, and belongs to the technical field of utilization of waste water obtained duringfood processing. The method comprises the following steps of after the soybean whey waste water is pretreated, extracting the lipoxygenase by an inverse pH gradient method, firstly removing hybridprotein at the pH of isoelectric points of the lipoxygenase, then separating the lipoxygenase at the isoelectric points of the lipoxygenase, and then combining ultrafiltration with gel chromatography toobtain refined lipoxygenase. The soybean whey waste water is used for obtaining the lipoxygenase high in industrial value, and the extraction rate of the lipoxygenase is high. The lipoxygenase is highin purity and good in enzymatic activity. The method is convenient to operate, safe, effective, and low in cost, and can be used for development and utilization of the lipoxygenase in the soybean whey waste water in industrial use so as to realize source recycling. The method has notable economic benefits and social benefits.
Owner:QILU UNIV OF TECH
Process for purifying bromelain by chelated metal affinity membrane
Owner:DONGHUA UNIV
Movable radioactive waste water far infrared treatment device
InactiveCN104299667AHigh purification factorEasy to moveRadioactive decontaminationAutomatic controlPollution dispersion
The invention discloses a movable radioactive waste water far infrared treatment device. The device comprises a transport cart, a heat-preserving cabin, a waste water treatment system, an automatic control device and an external pipeline. The heat-preserving cabin is arranged on the transportation cart. The waste water treatment system and the automatic control device are arranged in the heat-preserving cabin. The automatic control device is used for controlling the operation of the waste water treatment system. Radioactive waste water high in salt content and oil content can be treated, a purification coefficient is high, a safety coefficient is high, the device is small and high in mobility and applicability, and the technical effect of reducing pollution dispersal is achieved.
Owner:NUCLEAR POWER INSTITUTE OF CHINA
A kind of purification method of malt limit dextrinase
InactiveCN102268416AGood reproducibilityHigh purification factorHydrolasesAmmonium sulfateIon exchange
The invention discloses a purification method of malt limit dextrinase. The method includes: 1) Ammonium sulfate step-by-step precipitation: add ammonium sulfate with a saturation of 25-35% to the enzyme crude extract Ⅰ to remove impurity proteins, and then add ammonium sulfate to the supernatant to a saturation of 75-35%. 85%, salting out to get the crude enzyme solution II; 2) Ion exchange chromatography: After the filler is pretreated and packed, equilibrate with 15~20mmol / LTris-HCl buffer solution with a pH value of 7.0, load the sample, and then use 0~ Gradient elution with 1mol / L NaCl buffer solution; 3) Gel filtration chromatography: After the sample separated by ion exchange chromatography column is further purified by gel filtration chromatography column, it is freeze-dried to obtain a highly purified limit dextrinase product . The method has the advantages of simple process, safety, low cost, high product purity and good purification effect, and is an effective and reliable method for purifying limit dextrinase.
Owner:SOUTH CHINA UNIV OF TECH
Method for purifying xylanase
The invention belongs to the technical field of biological enzymes, and particularly relates to a method for purifying xylanase. The method disclosed by the invention takes coarse xylanase powder which is sold in the market as a raw material, and the raw materials can be produced through steps, including pH regulation, hollow fibrous membrane filtering, ultrafiltration, freeze drying and the like,to obtain purified xylanase. By use of the method disclosed by the invention, ultrafiltration stage treatment is adopted, a purification multiple and efficiency can be effectively improved, production cost is lowered, in addition, liquid nitrogen can be adopted to realize quick cryopreserving of enzyme liquid, enzyme crystal structure change and enzyme activity loss caused by slow pre-freezing can be reduced, and product performance is improved. The method disclosed by the invention has the characteristics of a simple production technology, convenience in production, a high product yield, a low equipment investment and operation cost, a high resource comprehensive utilization rate and the like. The xylanase product purified by the method disclosed by the invention has high activity, can meet the requirement of the medicine industry for high-purity enzyme preparations, and can be widely applied to industries, including medicines, food, chemical engineering and the like.
Owner:CHONGQING TECH & BUSINESS UNIV
A kind of separation and purification method of Trichoderma reesei recombinant t-pa
ActiveCN105754976BReduce operating costsEasy to operateHydrolasesMicroorganism based processesSalting outIon exchange
The invention relates to the field of separation of purification of t-PA, particularly to a separation and purification method of Trichoderma reeseire combination t-PA. The separation and purification method comprises the following steps: pretreating t-PA-containing crude enzyme liquid prepared by fermenting Trichoderma reesei; decoloring the crude enzyme liquid with D296 strong anion exchange resin column, eluting the crude enzyme liquid with a phosphatic buffer solution to obtain eluent, and salting out the eluent with ammonium sulfate to obtain precipitates; dissolving the precipitates with the phosphatic buffer solution, and performing dialysis desalting; separating and purifying desalted enzyme liquid with Q-Sepharose-High-Performance ion exchange chromatography, performing gradient elution, and collecting to obtain active component liquid; freeze-drying to obtain a t-PA finished product. The whole method is simple and feasible; the molecular mass A of the obtained t-PA is about 65,000; the isoelectric point is about 4.24; the specific activity is greater than 3,200 U / mg; the purification factor is greater than 51.5, and the recycling rate is over 75 percent.
Owner:HUNAN UNIV OF SCI & ENG
A method for extracting lactoperoxidase using ultrafiltration-assisted two-phase extraction technology
ActiveCN104450639BHigh recovery rateHigh purification factorOxidoreductasesFood additiveUltrafiltration
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
A system for purifying and treating tail gas of fluorination process
ActiveCN106582198BSimple structureSimple and fast operationDispersed particle separationEmission standardElution
The invention belongs to the field of uranium conversion, and particularly relates to a purification treatment system of fluorine-containing and uranium-containing process tail gas generated in the uranium hexafluoride production process. According to the provided fluorinated process tail gas purification treatment system, the purification treatment on harmful ingredients in the fluorinated tail gas generated in the uranium hexafluoride production process is realized by a charcoal adsorption-alkali liquor elution technology determined through the groping for a long time; the purified process tail gas reaches the emission standard. The technical method has the advantages that the system structure is simple; the operation is simple and convenient; the control conditions are easily realized; the security coefficient is high, and the like.
Owner:THE 404 COMPANY LIMITED CHINA NAT NUCLEAR
Purification method of acetylcholinesterase from earthworm serum
ActiveCN102660516BVitality has no effectKeep full structureHydrolasesWater bathsPurification methods
The invention provides a purification method of acetylcholinesterase from earthworm serum. The method comprises the following steps: placing earthworm in clean water for 24 hours to eliminate excrement in the earthworm body, and storing the earthworm in a refrigerator for later use; homogenizing with phosphoric acid buffer solution in an ice-water bath condition to prepare homogenate, centrifuging to obtain crude enzyme extract, and storing in the refrigerator; adding surfactant Triton X-114 solution and citric acid buffer solution into 100mu L of crude extract, and diluting with secondary water until the volume is 10mL, sufficiently oscillating and shaking uniformly; placing the solution in a constant-temperature water bath with the water bath temperature of 35 DEG C to balance for 9 minutes; and centrifuging for layering to obtain a lower product which is the purified acetylcholinesterase product. The purification extraction method of the acetylcholinesterase from earthworm serum has wide enzyme sources and low price, high sensitivity, simple and stable preparation process and low production cost.
Owner:武汉中科志康生物科技有限公司
Distillation-free concentration method for alcohol
InactiveCN102491875BEasy to operateSimple processHydroxy compound separation/purificationHigh concentrationAlcohol
Owner:DONGHUA UNIV
Purification process of zirconium in purex process plutonium purification cycle
Owner:CHINA INSTITUTE OF ATOMIC ENERGY
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