44results about How to "High purification factor" patented technology

The invention provides a preparation method of an earthworm extract for relieving cough, eliminating phlegm, anti-inflammation and anti-microbial. The extraction method provided by the invention is mainly to extract the earthworm homogenate through NaAC buffer solution of pH 4.8-5.4 and acetone. After repeated tests, the present invention optimizes the extraction process provided by the applicant of the present invention, which greatly shortens the preparation time of the earthworm extract from 42-45 hours to 15-18 hours; and the yield increases from 73% to 100%. 82%, the recovery rate of the total activity has been improved, the specific activity has been increased from 1141 to 4443, and the specific activity has been increased by 3.9 times; the purification factor has increased from 41 times to 158 times, and the purification factor of the deoxyribonuclease active ingredient has increased by nearly four times. The preparation method provided by the invention has simple process, low cost and is easy to be popularized and applied in industrialized production. The purity and activity of the product prepared by the preparation method provided by the invention are significantly higher than that of related similar products prepared by the existing method.

The invention relates to a method for purifying bromelain by means of a metal chelate affinity membrane. The method comprises the following steps that: (1) activated nylon membrane is modified through hydroxyethyl cellulose bonding; (2) the modified nylon membrane reacts with the mixed solution of epichlorohydrin, sodium hydroxide and sodium borohydride, and then the membrane is respectively dipped in the mixed solution of sodium carbonate, sodium borohydride and a coupling agent and a zinc sulfate solution, thereby generating a chelated zinc ionic metal affinity membrane; (3) the metal affinity membrane is piled up into a membrane stack, and is fed into a membrane bridge; a peristaltic pump pumps in a Tris-HCl buffer solution and an eluent and then pumps in a bromelain solution so as tocarry out elution by eluant, thereby generating bromelain; (4) enzymatic activity and protein content are tested to calculate purification multiple. The method has quickness, high efficiency, simplicity, convenience, great fractional dose and high activity of extracted enzyme, and is suitable for industrialscale production.

The invention relates to the field of separation of purification of t-PA, particularly to a separation and purification method of Trichoderma reeseire combination t-PA. The separation and purification method comprises the following steps: pretreating t-PA-containing crude enzyme liquid prepared by fermenting Trichoderma reesei; decoloring the crude enzyme liquid with D296 strong anion exchange resin column, eluting the crude enzyme liquid with a phosphatic buffer solution to obtain eluent, and salting out the eluent with ammonium sulfate to obtain precipitates; dissolving the precipitates with the phosphatic buffer solution, and performing dialysis desalting; separating and purifying desalted enzyme liquid with Q-Sepharose-High-Performance ion exchange chromatography, performing gradient elution, and collecting to obtain active component liquid; freeze-drying to obtain a t-PA finished product. The whole method is simple and feasible; the molecular mass A of the obtained t-PA is about 65,000; the isoelectric point is about 4.24; the specific activity is greater than 3,200 U / mg; the purification factor is greater than 51.5, and the recycling rate is over 75 percent.

The invention discloses a method for extracting lactoperoxidase by the ultrafiltration assisted two aqueous phase extraction technology. The method comprises the steps of using bovine whey as the raw material; enriching all lactoperoxidase from bovine whey into a salt phase by the two aqueous phase extraction technology; processing the salt phase solution through an ultrafiltration membrane to remove small impure protein; further purifying lactoperoxidase; desalting through a dialysis bag; then freezing and drying, so as to prepare a high-activity lactoperoxidase product which can be used in natural food additives. With the adoption of the method, lactoperoxidase with natural antibacterial activity can be directly prepared and directly added as a preservative into foods or toothpaste and other daily supplies.

The invention provides a purification method of acetylcholinesterase from earthworm serum. The method comprises the following steps: placing earthworm in clean water for 24 hours to eliminate excrement in the earthworm body, and storing the earthworm in a refrigerator for later use; homogenizing with phosphoric acid buffer solution in an ice-water bath condition to prepare homogenate, centrifuging to obtain crude enzyme extract, and storing in the refrigerator; adding surfactant Triton X-114 solution and citric acid buffer solution into 100mu L of crude extract, and diluting with secondary water until the volume is 10mL, sufficiently oscillating and shaking uniformly; placing the solution in a constant-temperature water bath with the water bath temperature of 35 DEG C to balance for 9 minutes; and centrifuging for layering to obtain a lower product which is the purified acetylcholinesterase product. The purification extraction method of the acetylcholinesterase from earthworm serum has wide enzyme sources and low price, high sensitivity, simple and stable preparation process and low production cost.

The invention relates to a distillation-free concentration method for alcohol, which comprises the following steps: 1, adding ammonium sulfate in an alcohol solution with the concentration of 20 to 30 percent, dissolving the ammonium sulfate through fully agitating, standing for layering to obtain an upper-phase solution and a lower-phase solution, and then removing the lower-phase solution and collecting the upper-phase solution; and 2, adding ammonium citrate in the upper-phase solution, dissolving the upper-phase solution through fully agitating, standing for layering to obtain another upper-phase solution and another lower-phase solution, and then removing the lower-phase solution and collecting the upper-phase solution, so as to obtain concentrated alcohol. The method provided by the invention has the advantages of convenience in operation, simple process and quickness in separation; and the alcohol obtained through the method has high concentration and wide application, and is convenient for large-scale industrialized production.

The invention belongs to the technical field of spent fuel post-treatment, and discloses a process for purifying zirconium in a Purex flow plutonium purification cycle. 30% tri-butyl-phosphate (TBP)-kerosene is adopted as an extraction agent 2AX in a 2A extractor, so as to recover uranium and plutonium and purify fission fragments. The process comprises an extraction section and a washing section; and the concentration of a nitric acid in a 2AF feed liquid is 3.5-3.7mol / L, and 2AS is an HNO3 solution of which the concentration is 0.8-0.85mol / L. The process has the advantages that the plutonium yield is greater than 99.9%, and the purification coefficient of zirconium is greater than 100.