64 results about "Antifungal protein" patented technology

An object of the present invention is to search and identify novel antifungal proteins capable of inhibiting the growth of plant pathogenic microorganisms including Magnaporthe grisea and Rhizoctonia solani causing two major rice diseases at relatively low concentrations, and further to clone a gene for said protein. The present invention provides an antifungal protein which can be obtained from fraction(s) precipitated by ammonium sulfate precipitation using an aqueous extract from Pleurotus cornucopiae, wherein said protein has an antifungal activity against at least rice blast, and exhibits existence of a component having a molecular weight of about 15 kDa as determined by SDS-PAGE method; a gene encoding said protein and uses thereof.

The invention relates to a class of antibacterial cationic reactive dyes and a preparation method thereof. The dye matrix is ​​an anthraquinone structure, the active group is a chloro-s-triazine or a fluoro-s-triazine, and the water-soluble group is a quaternary ammonium salt structure with a long carbon chain. The chemical structure is as follows: This type of dye can be used for cellulose fibers , protein fiber, polyamide fiber, acrylic fiber, cationic dyeable polyester fiber dyeing and antibacterial finishing, with high antibacterial effect, excellent color fastness, antibacterial durability. The preparation method of the dye includes: a. dissolving aminoanthraquinone A and trichloro or trifluoro-s-triazine in a nitrobenzene organic solvent, heating to 90°C, rising to 120°C after 1 hour, cooling and filtering to obtain the dye intermediate B; b. Dissolve the dye intermediate B prepared in step a in DMF organic solvent, add tertiary amine intermediate C, react at 55°C for 3-4 hours, evaporate the solvent DMF to obtain dye intermediate D, and dry it in vacuum c. Dissolve the dye intermediate D obtained in step b in DMF organic solvent, add haloalkyl E, heat to about 120°C, react for 4-5 hours, evaporate the solvent DMF, wash with ether, and recrystallize with ether-ethanol , Dye F was obtained and dried in vacuum. When this type of dye is used for textile dyeing, it can also endow textiles with certain functions, and has the advantages of water saving, energy saving, and ecological protection.

The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.