64 results about "Antifungal protein" patented technology

The invention specifically discloses a microbial inoculant, in particular to a microbial inoculant prepared from swine borne bacillus licheniformis YHSH-02 and the application thereof in livestock breeding. The culture is preserved in CGMCC, 3#, Yard #1, West Beichen Road, Chaoyang District, Beijing, with the preservation number of CGMCC NO. 15454 and the preservation date of 15th, March, 2018. The bacillus licheniformis YHSH-02 is separated from a swine intestinal tract, can generate rich lipopeptides bacilli, antifungal proteins and the like, and can inhibit escherichia coli, pseudomonas aeruginosa, enterococcus faecalis, enterobacter cloacae, pseudomonas aeruginosa, proteusbacillus vulgaris, staphylococcus aureus, salmonella and clostridium. The bacillus licheniformis YHSH-02 obtained from the swine intestinal tract is a biological control potential culture which is high in preventing and treating efficiency and wide in preventing and treating range and can promote growth obviously,and has a good application and development prospect in non-resistant breeding.

The invention discloses yersinia pseudotuberculosis-sourced antifungal protein and a coding gene and application thereof, and belongs to the technical field of genetic engineering. The antifungal protein is protein comprising the amino acid sequence shown as SEQ ID NO: 1, or protein obtained by substituting, deleting or inserting one or several amino acid sequences in the amino acid sequence shownas SEQ ID NO: 1 and having equivalent activity. The invention further provides the coding gene, an expression vector and engineering bacteria of the antifungal protein. The invention further providesa separation and purification method of the yersinia pseudotuberculosis-sourced antifungal protein. The protein is applied to an antifungal agent and the coding gene of the protein is applied to a transformed plant. The YPK_AFP1001 antifungal gene and the protein, related by the invention, have higher and more broad-spectrum antifungal activity.

The present invention belongs to the field of gene engineering technology, and especially a kind of active promoter segment F1 of Bacillus pumilus, its fusion gene constructing body and new recombinant expression vector carrying the constructing body. The present invention also relates to the said random alveolus mutation, antifungal protein acquisition, expression vector transformed Bacillus pumilus cell and transformed cell generated antifungal function. The said promoter activity improvement can resist chloromycetin as high as 900 mcg / ml, and the obtained antifungal protein secretion obtained under the directing of the promoter has certain antifungal characteristic.

The invention discloses an application of a domestic silkworm cocoon antifungal protease inhibitor in fungus prevention and a separation method of the domestic silkworm cocoon antifungal protease inhibitor. The separation method comprises the following steps: layering and cutting a silkworm cocoon into pieces, then adding 100mm of Tris-HCL buffer solution with pH of 7.5, and extracting for 30 minutes under the condition that the temperature is 37 DEG C and the rotation speed is 220 r / pm, centrifuging for 15 minutes under the rotation speed of 10000rpm, filtering supernatant by utilizing a 0.22-micrometer filter membrane, to obtain the domestic silkworm cocoon antifungal protease inhibitor. The prepared protease inhibitor can inhibit the activity of fungal protease secreted by Tritirachium album limber, can inhibit the spore germination of silkworm pathogenic fungal beauveria bassiana and still can maintain the activity after being incubated for 10 minutes in boiled water. The prepared domestic silkworm cocoon antifungal protease inhibitor belongs to the natural, high-activity and high-stability antifungal protease and is non-toxic and harmless; and meanwhile, the silk used in the method is rich in resource, and a novel way is provided for diversified development of the silk.

The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.

An object of the present invention is to search and identify novel antifungal proteins capable of inhibiting the growth of plant pathogenic microorganisms including Magnaporthe grisea and Rhizoctonia solani causing two major rice diseases at relatively low concentrations, and further to clone a gene for said protein. The present invention provides an antifungal protein which can be obtained from fraction(s) precipitated by ammonium sulfate precipitation using an aqueous extract from Pleurotus cornucopiae, wherein said protein has an antifungal activity against at least rice blast, and exhibits existence of a component having a molecular weight of about 15 kDa as determined by SDS-PAGE method; a gene encoding said protein and uses thereof.

The invention relates to a class of antibacterial cationic reactive dyes and a preparation method thereof. The dye matrix is ​​an anthraquinone structure, the active group is a chloro-s-triazine or a fluoro-s-triazine, and the water-soluble group is a quaternary ammonium salt structure with a long carbon chain. The chemical structure is as follows: This type of dye can be used for cellulose fibers , protein fiber, polyamide fiber, acrylic fiber, cationic dyeable polyester fiber dyeing and antibacterial finishing, with high antibacterial effect, excellent color fastness, antibacterial durability. The preparation method of the dye includes: a. dissolving aminoanthraquinone A and trichloro or trifluoro-s-triazine in a nitrobenzene organic solvent, heating to 90°C, rising to 120°C after 1 hour, cooling and filtering to obtain the dye intermediate B; b. Dissolve the dye intermediate B prepared in step a in DMF organic solvent, add tertiary amine intermediate C, react at 55°C for 3-4 hours, evaporate the solvent DMF to obtain dye intermediate D, and dry it in vacuum c. Dissolve the dye intermediate D obtained in step b in DMF organic solvent, add haloalkyl E, heat to about 120°C, react for 4-5 hours, evaporate the solvent DMF, wash with ether, and recrystallize with ether-ethanol , Dye F was obtained and dried in vacuum. When this type of dye is used for textile dyeing, it can also endow textiles with certain functions, and has the advantages of water saving, energy saving, and ecological protection.

The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences of Monascus pilosus BCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained from M. pilosus culture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such as Paecilomyces variotii BCRC 33174 and Helminthosporium panici BCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in other Monascus species such as M. Barkeri, M. floridanus, M. lunisporas, M. pilosus, M. ruber and the like. It is also been proved that the mafp1 gene and cDNA thereof in four Monascus strains, M. pilosus (BCRC 38072, BCRC 38093 and BCRC 31502) and M. ruber BCRC 31533, have the same DNA sequences.

The invention provides an application of anthraquinone compound in preparing an antifungal drug and an antifungal composition, which belongs to the technical field of medicine. The anthraquinone compound is 9,10-dioxo-N-(5-phenylthiazole-2-groupyl)-9,10-dihydroanthracene-2-sulfonyl; and an in vitro cell experiment proves that the anthraquinone compound has good antifungal effect for various clinical fungi strains, the application dosage of the antifungal drug can be obviously reduced, the inhibition effect for the drug-resistant fungi can be improved, and the effect of the antifungal drug for the drug-resistant fungi can be restored.