The invention provides a high-efficiency three-dimensional green intelligent mushroom cultivation room, and belongs to the field of three-dimensional intelligent mushroom cultivation in an original ecological environment under an imitating wild environment. A mushroom cultivation medium is spread on a mushroom support device in a thermal insulation cultivation room, and various fungus mushrooms oftoadstool, ycoperdon bovista, colorful mushroom, colorful lotus mushroom, shiitake, white beech mushroom, mushroom, drumstick mushroom, flammulina velutipes, red fir fungus, pleurotus cornucopiae, agrocybe cylindracea, ganoderma lucidum, hericium erinaceus, pleurotus ostreatus, tricholoma gambosum, pleurotus nebrodensis, pleurotus eryngii, flower mushroom, crab mushroom, and pleurotus geesteranusare cultivated through supplementing nutrition to the mushroom cultivation medium using the irrigation mode of infiltrating nutrient liquid from inside a nutrient supplying cavity to the outer side.The cultivation method of various mushrooms is simple and easy to operate, large-scale cultivation and industrialization are facilitated, a variety of mushrooms can be cultivated in four seasons, andthe supply of all kinds of mushrooms in the market in four seasons is ensured; the cultivated mushrooms are high in production yield, good in quality, and high in commercial value of industrialization; the mushroom cultivation medium can be repeatedly used for cultivating mushrooms, and the cost of mushroom cultivation is reduced.