127 results about "Magnaporthe grisea" patented technology

The invention relates to the technical field of plant genetic engineering, in particular relates to isolating clone and functional verification of a DAN fragment containing a gene OsWRKY45-2 related to rice disease resistance. The gene OsWRKY45-2 codes WRKY proteinoids and can endow rice with resistance to the diseases caused by bacterial pathogenic bacteria, namely the leaf blight bacteria (Xanthomonas oryzae pv.oryzae) and fungal pathogenic bacteria, namely the rice blast bacteria (Magnaporthe grisea). The fragment and the exogenous regulatory sequence thereof are directly transferred into rice, thus the resistance capability of transgenic rice with over-expression OsWRKY45-2 against bacterial blight in and rice blast is improved remarkably.

The invention relates to primers for molecular detection of nontoxic genes of Magnaporthe grisea and a use method thereof, is used for rapid molecular detection of nontoxic genes Avrpik and Avrpizt of Magnaporthe grisea, and belongs to the fields of crop disease control and breeding for disease resistance. According to the invention, two pairs of primers Avrpik-F/R and Avrpizt-F/R are designed, and used for respectively detecting the nontoxic genes Avrpik and Avrpizt of Magnaporthe grisea, wherein the lengths of the specifically amplified DNA (Deoxyribonucleic Acid) fragments are 184 bp and 153 bp respectively. The primers are suitable to be used for analyzing nontoxic gene composition of pure Magnaporthe grisea culture and Magnaporthe grisea colony from disease position.

The invention discloses a new Pseudomonas corrugate strain (P94 GMCC No.1895), which is characterized by the following: manufacturing relative HCN to prevent bacteria, proteinase and phospholipase and IAA; inhibiting partial plant pathogenic fungus (Botrytis cinerea, Ceratocystis fimbriata, Monilinia laxa, Magnaporthe grisea, Pythium aphanidermatum and Phytophthora capsici) and partial pathogenic bacteria (Pseudomonas syringae, Acidovorax avenae and Ralstonia solanacearum); accelerating the growth of tomato and cucumber to improve production by 22%-28%.

The present invention discloses a plant pesticide which restrains the rice blast and the application of the plant pesticide for restraining rice blast. The plant pesticide is a low-toxicity and high-efficient novel biological product which is developed by using the plant of Chinese scholar tree as raw material. The pesticide is applied with a mode of foliage spray and can be applied to the agricultural field. The plant pesticide for restraining the rice blast according to the invention is obtained through the following steps: using the Chinese scholar tree as raw material, putting the raw material into an extraction kettle after breaking the tissue of raw material, adding the organic solvent with the weight of 2-3 times of the weight of raw material, immersing and extracting for 12-24 hours in the condition of room temperature, extracting for 2-3 times; condensing the extracted liquid to solid-state concentrate; dissolving the solid-state concentrate into the organic solvent with the weight of 0.5-1.5 times of the weight of solid-state concentrate, adding the emulsifying agent with the weight of 0.1-0.15 times of the weight of solid-state concentrate, sufficiently mixing, adding the water with the weight of 20-40 times of the weight of solid-state concentrate, and the plant pesticide aqueous emulsion for restraining rice blast is prepared. The plant pesticide aqueous emulsion for restraining rice blast can effectively restrain the magnaporthe grisea, increases the quality of arable farming and strengthens the capability of crop for resisting disasters and pests.

The invention relates to the technical field of plant gene engineering, in particular to separation, cloning and function verification of a DNA fragment containing rice disease resistance related genes GH3-2. The genes GH3-2 code auxin amide synthetase. The genes GH3-2 can endow the rice with function of resisting the diseases caused by bacterial pathogenic bacteria Xanthomonas oryzae pv. oryzae, bacterial Xanthomonasoryzae pv. oryzicola and fungal pathogenic bacteria Magnaporthe grisea. The fragment and the exogenous adjusting sequence thereof are directly transferred into the rice, and the transgenic rice for excessively expressing the GH3-2 has the remarkably enhanced resistance to the Xanthomonas oryzae pv. oryzae, bacterial Xanthomonasoryzae pv. oryzicola and the Magnaporthe grisea.

The invention relates to a biocontrol pseudomonas sp. and an application thereof, specifically to a biocontrol strain GZ9 with a preservation number of CCTCC M 20191037. The strain has a significant inhibition effect on botrytis cinerea, and shows a good inhibition effect on phytophthora capsici, magnaporthe grisea and gibberella zeae. The fermentation liquor of the strain has a good control effect on tomato gray mold, and can reach a control effect of 78.66%. Thus, the strain has good biocontrol application prospect.

The invention relates to a phenylimidazole derivative, a synthesis method of the phenylimidazole derivative and application of the phenylimidazole derivative to pesticide, particularly application tothe fungicide pesticide aspect, and belongs to the technical field of pesticide. In order to solve the technical problems, the invention provides a novel phenylimidazole derivative, a synthesis methodof the novel phenylimidazole derivative and application of the novel phenylimidazole derivative to the pesticide. The structure of the compound is shown as a formula I. The compound structure is simple and novel; the synthesis is easy; meanwhile, the fungicidal activity is realized; a better bacteriostasis or sterilization effect is achieved on important plant pathogenic fungi such as tomato early blight, tomato botrytis cinereal, cucumber fusarium oxysporum, magnaporthe grisea and rice rhizoctonia solani.

The invention discloses a method for detecting magnaporthe grisea genes for enhancing magnaporthe grisea strain pathogenicity, and relates to the field of plant protection and biological technology. The method comprises the steps that a reaction of resistance or susceptibility and a real-time RT-PCR are inspected for analyzing defense related gene expression of rice early stage response and a qPCR is adopted for detecting the relative fungus growth amount of disease spots; a prokaryotic expression product of the genes is sprayed to leaves for 24 h on the basis of the concentration being 1.0 microgram/ml-6.0 microgram/ml, and then strong/weak pathogenic strains are inoculated; the reaction of resistance or susceptibility and the real-time RT-PCR are inspected for analyzing rice defense related gene expression; overexpression strains of the genes are utilized for being inoculated on rice blades in an in-vitro mode, and the reaction of resistance or susceptibility and the real-time RT-PCR are inspected for analyzing the rice defense related gene expression and the qPCR is inspected for analyzing relative fungus growth amount on disease spots. The method can comprehensively and accurately clear pathogen effect protein genes and improve the pathogen infective ability, and the accurate and comprehensive method is provided for determining the pathogen effect protein for improving the pathogen infective ability in the future.

The invention belongs to the technical field of genetic engineering and specifically relates to a rice blast resistance gene RMg4 or RMg5 or RMg6 and an application thereof. The invention discloses a nucleotide sequence of the rice blast resistance gene RMg4 or RMg5 or RMg6 and an encoded amino acid polypeptide sequence thereof. The three genes belong to members of an NBS-LRR (nucleotide-binding site and leucine-rich-repeat) type anti-disease gene family. The three blast resistant genes disclosed by the invention are cloned from a rice line expressing high resistance to magnaporthe grisea and converted to a magnaporthe grisea-susceptible variety, a magnaporthe grisea infection method is utilized for assessing the resistance against diseases of the genes, and then the three genes can be finally determined to have the resistance to rice blast.

The invention discloses a pathogenic protein MoSNT2 derived from Magnaporthe grisea, the protein is composed of the amino acid sequence shown in SEQ ID No: 1; the invention also discloses the gene MoSNT2 encoding the pathogenic protein, the gene It consists of the nucleotide sequence shown in SEQ ID No:2. The invention also discloses the use of the above-mentioned gene or protein as a target for designing or screening antifungal drugs. The invention provides an important target gene or target protein for the control of rice blast. The drug developed by using the gene or protein as a target can destroy the gene expression or protein function of the rice blast fungus, and can effectively control the pathogenicity and prevalence of the rice blast fungus.

The invention relates to a method for separating and screening out an antagonistic endophytic bacterium from a lycoris aurea bulb. According to the method, by using an endophyte separation method, an endophytic bacterium is obtained by separating the endophytic bacterium from the lycoris aurea bulb; an improved confrontation culture method is adopted, test magnaporthe grisea is dripped in the center of a flat plate, and is cultured for 2-3 days; four crosswise corners around the magnaporthe grisea are inoculated with four bacterial strains respectively, and culture is carried out for 5-6 days; through observation, a bacterium having a bacteriostatic effect is the antagonistic bacterium obtained through screening. The antagonistic bacterium having an obvious inhibiting effect on magnaporthe grisea of rice is obtained; an antimicrobial spectrum experiment proves that the antagonistic bacterium also has an inhibiting effect on fusarium graminearum, aspergillus fumigatus and candida albicans. By adopting the method disclosed by the invention, the screening efficiency can be remarkably improved, and the antagonistic bacterium obtained by separation can be used as a biocontrol bacterium for being prepared into a microbial preparation, thus providing a basis for biological control over diseases.

The invention discloses a peganum harmala lipid transfer protein (PHP) and relates to the preparation and use of new protein with antifungal, anticancer and antivirus activities. The method uses seeds of a peganum harmala plant as raw materials and comprises the following steps: preparing a coarse product, performing cationic exchange chromatographic separation and purifying by using a molecular sieve; and gradually separating and collecting a purified component having antibacterial activity, dialyzing, desalting, freeze-drying and thus, obtaining purified PHP. In the invention, a filter paper disc agar diffusion method is adopted, and researches prove the protein has growth inhibiting effect on five plant pathogenic fungi, namely alternaria alternate, penicillium degitatum, rhizopus stuolonifer, magnaporthe grisea and penicillium italicum. An 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method proves that the protein has inhibiting effect on proliferation of human esophageal carcinoma cell Eca109, human cervical cancer cells HeLa, human stomach cancer cells MGC-7, mouse melanoma cells B16 and the like. The protein can inhibit the activity of human immunodeficiency virus 1 (HIV-1) reverse transcriptase. The protein can be used for developing medicines for resisting agricultural pathogenic fungi, tumors and viruses.

The invention discloses a new necessary MgPTH12 gene and utility to make pear spore bacterium attach to mature and lead to disease, which is characterized by the following: the gene and its cDNA and coding product possess the sequence in the SEQ ID No.:1, No.:2 and No.:3; the protein of coded gene possesses homeo structural region, which is not similar to known functional protein; the gene removes abnormal and matured attaching cell, which reduces forming frequency of infecting tin and extending infecting hypha without pathogenic ability for rice; the homologous gene of gene lies in the plant pathogenic bacteria widely, which can be applied to design and sieve new drug to prevent fungus.

The invention belongs to the technical field of pesticide and relates to a broad spectrum fungicide, in particular to a farming fungicide comprising Azoxystrobin. The invention consists of the original pesticide of the Azoxystrobin, an accessory ingredient and an excipient; and microemulsion and missible oil are manufactured by a normal production technique. The Azoxystrobin is a methoxy acrylate fungicide developed by Syngenta Company, is used for controlling powdery mildew, rust disease, glume blight, scab, downy mildew and Magnaporthe grisea on cereal crops, fruit trees and vegetables, and has specific effects on powdery mildew of cucumber, downy mildew of grape and apple scab.

The invention provides a rice induced disease-resistant gene OsAAA1 which is doubly induced by salicylic acid and magnaporthe grisea. The nucleotide sequence of the rice induced disease-resistant gene OsAAA1 is as shown in SEQ ID NO:1. The amino acid sequence of a rice disease-resistant protein coded by the rice induced disease-resistant gene OsAAA1 is as shown in SEQ ID NO:2. The rice induced disease-resistant gene OsAAA1 provided by the invention can be applied to breeding of a rice variety with resistance to rice blast and bacterial blight. A specific application mode is as follows: an excessive expression vector is constructed according to the rice induced disease-resistant gene OsAAA1 provided by the invention; the excessive expression vector is transferred into a receptor variety by utilizing agrobacterium-mediated genetic transformation. The variety shows the antibacterial property for magnaporthe grisea and xanthomonas oryzae under the induction of salicylic acid.

The invention relates to a method for treating paddy rice tissues by using a prokaryotic expression product of a magnaporthe grisea effect protein gene and inducing generation of callose, belonging to the technical field of plant protection and biology. The method is an improvement on the prior art, comprises a prokaryotic expression technology which is the same as that of the conventional method, a protein purifying technology, the wavelength ranges of exciting light and emitted light of a used fluorescence microscope and an aniline blue dyeing method, and is characterized by comprising the following steps of: treating the root tissues of Nipponbare serving as a disease-resistant paddy rice variety with the prokaryotic expression product which is 1.0-10.0 mg/ml in concentration of the magnaporthe grisea effect protein gene is used for treating the root tissues for 12 hours; performing aniline blue dyeing; and directly observing the formation of callose under the fluorescence microscope. The method is easy, convenience, accurate and rapid; and a large amount of spontaneous fluorescence interference can be avoided effectively, and the situation of basic defense reaction of paddy rice can be obtained directly and qualitatively.

The invention relates to the technical field of gene engineering, and particularly discloses magnaporthe oryzae avirulence gene AvrPib and application thereof. The avirulence gene comprises a DNA (deoxyribonucleic acid) sequence, with a promoter, as shown in SEQIDNO:1 or a cDNA (complementary deoxyribonucleic acid) sequence as shown in SEQIDNO:2. Molecular targets of a novel pesticide can be designed on the basis of the structure of the gene and application test results thereof; by covalently introducing the gene and relevant anti-disease genes into host plants, such as rice, new disease-resistant varieties can be bred; specific molecular markers produced according to the gene sequence can be applied to monitoring field magnaporthe grisea groups; monitoring results obtained can be used for guiding the reasonable layout of the disease-resistant varieties; the gene has a wide application value, and has great significance in rice production and breeding research.