Application of biopesticide tetramycin in preventing and controlling Magnaporthe grisea
A technology of rice blast and wuningmycin, which is applied in the directions of chemicals, applications, biocides for biological control, etc., can solve the problems of chemical residues, no other, environmental pollution, etc.
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Embodiment 1
[0122] Embodiment 1: the preparation of Wuningmycin fermented liquid
[0123] (1) Seed fermentation medium: every 100 ml contains: soybean cake powder 1.0g, starch 1.0g, glucose 0.7g, peptone 0.5g, magnesium sulfate 0.03g, ammonium sulfate 1.0g, dipotassium hydrogen phosphate 0.02g, Calcium carbonate 0.7g, sodium chloride 0.1g, vegetable oil 0.2g, pH natural;
[0124] (2) Submerged fermentation medium: every 100 ml contains: glucose 2.5g, starch 1.5g, cornmeal 1.0g, soybean cake powder 2.0g, peptone (or corn steep liquor) 0.5g, ammonium sulfate 0.2g, magnesium sulfate 0.03g, 0.02g of dipotassium hydrogen phosphate, 0.7g of calcium carbonate, 0.1g of sodium chloride, 0.2g of vegetable oil, after sterilization, add 0.4% of the medium volume of n-propanol, and the pH is natural.
[0125] (3) Preparation of seed fermentation broth: get Streptomyces anygroscopicus subsp. Wuzhouensis n.subsp strain at an inoculum size of 5-20%, and cultivate it in a seed fermentation medium at 25-3...
Embodiment 2-11
[0127] Example 2-11: Application example of Wuningmycin for controlling rice blast
[0128] Statistical table of raw data of field efficacy test of Wuningmycin on rice blast control (Yiquan Town, Donggang City, Dandong City, Liaoning Province)
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[0131] Statistical table of raw data of field efficacy test of Wuningmycin on controlling rice blast (experimental field of Agricultural Technology Center, Fujin, Heilongjiang)
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