1974 results about "Biopesticide" patented technology

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

The invention discloses a biopesticide bactericide prepared from Chinese herbs and the use of the biopesticide bactericide. The bactericide is prepared from the following components in percentage by weight: 45%-55% of garlic extract, 15%-25% of heartleaf houttuynia herb extract, 10%-20% of purslane extract, 8%-15% of extract of folium artemisiae argyi, gingko and sweet wormwood, and 3%-5% of sulfur powder. The biopesticide bactericide prepared from Chinese herbs has a strong killing effect on pathogenic bacteria of pythium rot, damping off, rice blast and the like of crops such as rice, wheat and vegetables; the bactericide is capable of achieving the purpose of preventing and treating fungus and bacterial disease, and also capable of promoting the expression of a defensive enzyme system in the bodies of plants, improving the disease resistance of the plants and promoting the increase of production of crops; meanwhile, the prevention and treatment effect of the bactericide on soil-borne diseases propagated via soil is above 90%.

The present invention utilizes the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect attractants and / or pathogens. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material, attracting the insect and optionally introducing insect-specific pathogenic fungi. More than one fungus and substrate can be used in combination. The matrix of preconidial fungi can optionally be dried, freeze-dried, cooled and / or pelletized and packaged and reactivated for use as an effective insect attractant and / or biopesticide. Attractant extracts of the preconidial entomopathogenic mycelium are disclosed.

The invention relates to an ecological agricultural drug and a preparing method compounded by a content of Chinese traditional herb plant insecticide and amino group oligosaccharide. The ecological agricultural drug is refined by blending and processing the plant gradients containing one or a plurality of insecticide and disinfecting components and the Chitosante raw materials and solutions which are extracted with the amino group oligosaccharide. The weight percentages of all components are respectively: five to thirty percent of plant materials, seventy to ninety five percent of Chitosante material, and the solvents are alcohol and water. When in blending and configuring process, additives are added; wherein, the additives are one or a plurality of the agents, such as an antiseptic agent, a dispersing agent, a surface active agent, a penetrant or an emulsifying agent. The plant materials are materials such as Sophora flavescens, tobacco, Indian chinaberry fruit, pyrethrum, climbing groundsel or Aztec marigold, etc., the invention has the beneficial effects of multifunction of insecticide and disinfection, anti disease, disease prevention; easy degradation of the product, no residues and extensive raw material source. The working process of the invention simplifies the processes of extraction, condensation and filter with low producing cost.

The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9> / mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.

The invention relates to microcapsules prepared from viable microbes such as pseudomonas, bacillus and paenibacillus serving as main active components, belonging to the technical field of microorganism and biochemistry. The method for preparing microcapsules of viable microbe biopesticide comprises the following steps of: adsorbing microbial fermentation culture solution through an adsorption effect to prepare capsule cores and solidifying the capsule cores, and adding wall materials, so as to prepare a microcapsule preparation. The microcapsule preparation prepared by adopting the method has high content of viable microbes, high storage property and strong high-temperature and ultraviolent resistances, and stable and good prevention and control effect on plant diseases and insect pests.

The invention provides a tobacco matrix composite biopesticide and a preparation method thereof. The composite biopesticide which comprises at least a tobacco botanical insecticidal component and at least a microbial insecticidal component is prepared through fermenting insecticidal microbes with selecting a tobacco waste as a raw material. The preparation method comprises the following operationsteps: preparation of a medium, amplification culturing of spawns, fermentation of the biopesticide, and extraction. A fermentation technology can adopt liquid state fermentation or solid state fermentation. The tobacco matrix composite biopesticide of the invention which not only contains the insecticidal microbes and metabolites thereof, but also contains tobacco insecticidal components (nicotine and the like for example) has dual insecticidal effects. The method of the invention which allows resources of the tobacco waste which is not reasonably utilized at present to be fully utilized allows grain resources to be saved, and good economic benefits, ecological benefits and social benefits to be generated.

The invention relates to a multi-effect biological pesticide aqueous solution agent and a preparation method thereof. The multi-effect biological pesticide aqueous solution agent is characterized in that the agent contains 5-50 percent of pesticide active ingredient plant essence oil, 5-50 percent of simple organic carboxylic acid with a synergistic effect, 5-50 percent of pesticide assistant and 10-85 percent of water, wherein the plant essence oil is one or a mixture of wintergreen oil, camphor tree oil, clove oil, peppermint oil, eucalyptus oil, citionella oil, tea tree oil, lemongrass oil and cinnamon oil, the simple organic carboxylic acid with the synergistic effect is one or a mixture of formic acid, acetic acid and propionic acid, and the pesticide assistant is one or a mixture of dodecylbenzene sulfonic acid, Tween-80, Span-80 and OP-10. The agent can be used for insect killing, insect expelling, sterilization and antivirus in mosquito repelling, family agriculture, family flowering, organic agriculture and green agriculture.

The invention discloses a compound plant source agricultural bactericide and manufacturing method, which is characterized by the following: boiling traditional Chinese medicine with chenopodium ambrosioides, sickle wormwood, toosendanin and natural tanning; targeting plant fungus; displaying strong sterilizing specificity for cucumber, strawberry, tomato and potato.

The invention relates to a natural green biology organic pesticide composition and use thereof, wherein the technique features are: A producing natural biology pesticide using natural camphor, rhubarb, cinnamon, oleum eucalypti, ginger, common fennel, red pepper and auxiliary material; B producing natural biology pesticide using atractylodes rhizome,magnolia bark (prepared with ginger), tuckahoe,dried pinellia, patchouli oil, dried old orange peel, dahurian angelica root, areca peel, licorice extract, purple operilla leaf oil and auxiliary material. The pesticide can be directed applied on melon type, fruit type, leaf type vegetables, leguminous vegetables, tea leaf, traditional Chinese medicinal material, flower, fruit, rape, cotton, tobacco to control the pest. The vegetable and fruit using the biology organic pesticide can be directly eaten without residual pesticide harm to human.

Disclosed herein are pest controlling compositions (i.e., biopesticides) comprising one or more entomopathogenic fungi which. Further disclosed are methods of using such compositions for controlling invasive pests, particularly agriculturally relevant pests.

A wettable powder of bacillus thuringiensis and beauveria bassiana relates to a mixed biopesticide, aiming to the shortcomings of prevention and treatment limitations of beauveria bassiana and bacillus thuringiensis (Bt), particularly poor quick-acting property of microbial pesticides. The wettable powder provided in the invention is prepared from the following components: 10-14wt.% of bacillus thuringiensis, 4-14wt.% of beauveria bassiana, 3-5wt..% of wetting agent, 10-20wt.% of dispersant, 0.1-0.5wt.% of ultraviolet protectant and 50-60 wt.% of carrier. The wettable powder of bacillus thuringiensis and beauveria bassiana provided in the invention is high in activity, stable in effect, long in persistence and convenient for use.

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

The invention discloses a strain of bacillus subtilis and application thereof. The bacillus subtilis is Bs-03 CGMCC No. 3038 and can be used as a biological fertilizer and a biological pesticide whichcan prevent and control various diseases of plants. Experiments indicate that fermentation liquid of the bacillus subtilis Bs-03 CGMCC No. 3038 has significant effect on preventing and controlling verticillium dahliae in cotton, and cotton grows vigorously without causing phytotoxicity because of using the bacillus subtilis Bs-03 CGMCC No. 3038.

The invention discloses a highly-efficient planting method of dragon fruits. The highly-efficient planting method of dragon fruits comprises following steps of: (1) standing columns; (2) applying fertilizers; (3) performing field planting; (4) pinching; (5) topdressing; (6), performing pollination; (7) pruning branches; and (8) performing disease and pest control. The highly-efficient planting method of dragon fruits has following beneficial effects: the highly-efficient planting method combines organic fertilizers with inorganic fertilizers by reasonable fertilizing in order to increase utilization rate and decrease manufacturing cost of fertilizers; nutrition demands in different periods of dragon fruits are satisfied; biopesticides are adopted during an overall process in order to prevent diseases and pests without using any pesticide so that fruit without pollution can be made; the highly-efficient planting method helps improve yield and quality of dragon fruits and generates good economic benefit and is worth of promotion.

The invention discloses a bacillus amyloliquefaciens and application thereof in prevention and treatment of walnut anthracnose. The strain is bacillus amyloliquefaciens strain SDF-005 (preservation number CGMCC (China General Microbiological Culture Collection Center) No.6672). The strain, fermentation liquid prepared from the strain or bacterium agent prepared from the strain can serve as a biopesticide for the prevention and treatment of the walnut anthracnose. As shown in an experiment, the bacillus amyloliquefaciens strain SDF-005 disclosed by the invention has the characteristics of novelty, high efficiency and high living bacteria; wettable powder of the bacillus amyloliquefaciens strain SDF-005 has an obvious effect on the prevention and treatment of the walnut anthracnose with the prevention and treatment effect reaching 78.22%; and walnut can grow well without the production of phytotoxicity.

The present invention relates to biological bacillus lipopeptide pesticide and its application, and belongs to the field of biotechnology. The lipopeptide pesticide is prepared through fermentation culture in culture medium of glucose, cane sugar or corn slurry at temperature of 30-37 deg.c, rotation speed of 300-1000 rpm and dissolved oxygen of 30% for 30-36 hr; centrifugation to collect supernatant, adding HCl to regulate pH to 2.0-2.5, centrifugal extraction to collect supernatant, methanol or ethanol extraction, merging extracted liquid, and vacuum drying. The present invention is used in the green prevention and control of pests and diseases of field.

The present invention belongs to the field of agricultural cultivation technology, and particularly relates to a high-yield cultivation method of organic selenium-enriched rice. The method includes the following steps: A, selecting rice seeds and sterilizing the rice seeds; B, arranging rice seedling beds; C, sowing the rice seeds and cultivating rice seedlings; D, preparing a large field; E, transplanting the rice seedlings; F, managing the field; and J, harvesting the rice. Insect disinfestation for the rice seeds is carried out using Tripterygium wilfordii extracts; meanwhile, Tripterygium wilfordii extracts are used to dissolve the selenium and biological pesticides in the rice production process, and further improvements on the prevention and control of plant diseases and insect pests are realized in the overall growth process of the organic selenium-enriched rice. Instead of chemical fertilizers, bio-organic fertilizers are used in the rice cultivating process, which ensures a variety of nutrients required in the rice cultivating process and improves the rice yields. The rice yields and the selenium content of the organic selenium-enriched rice are higher than those of rice planted with traditional cultivation technology, and pesticide residues are significantly lower than that of the rice planted with the traditional cultivation technology.

The invention relates to a bacillus subtilis strain, a preparation prepared from the bacillus subtilis strain, a preparation method of the preparation, application of the bacillus subtilis strain to preparation of a preparation for preventing and treating crop mycete diseases and application of the preparation to prevention and treatment of the crop mycete diseases, and belongs to the technical field of biopesticide. The bacillus subtilis with the biological name of bacillus subtilis was collected in China General Microbiological Culture Collection Center (CGMCC) on December 26, 2012 (the address is Rentun Road, Chaoyang District, Beijing and the postal code is 100101), wherein the collection number is CGMCC No.5652. An in vitro living plant test shows that the bacillus subtilis preparation has a certain effect of preventing and treating crop mycete diseases and particularly has an obvious effect of preventing and treating peronospora sparsa disease of crops.

The present invention relates to a bio-pesticide comprising a mixture of natural spices that kills insects through direct contact. Piper Nigrum, Cuminum Cyminum, Cinnamomum Verum, Allium Sativum, Coriandrum Sativum, Curcuma, Foeniculum Vulgare, Trigonella Foenum Graecum, Syzygium Aromaticum, Capsicum, Zingiber Officinale, Murraya Koenigii, Brassica Nigra, and Sodium Chloride are mixed with potting soil and applied to a plant. The bio-pesticide works through the processes of repellency, digestive inhabitancy, paralysis and suffocation. The bio-pesticide can be applied as a dust or liquid spray to the plants, and can eliminate the pests within 48-72 hours.

The invention provides a compound Chinese herbal botanical insecticide-fungicide. The botanical insecticide-fungicide comprises the following raw materials in parts by mass: 70-90 Celastrus Angulatus, 20-30 parts of golden larch bark, 30-50 parts of Cnidium Monnieri, 60-80 parts of Radix stemonae, 20-30 parts of Radix Euphorbiae Lantu, 10-30 parts of betel nut, 50-70 parts of thunder god vine, 30-50 parts of Radix Sophorae Flavescentis, 65-95 parts of CAOWU and50-80 parts of tobacco leaves. The product disclosed by the invention can kill insects and bacteria, has multiple functions and has higher efficiency compared with commercially available biological pesticides. The time from spraying the insecticide-fungicide to the death of insects is 40 hours earlier and the death rate of insects within 24 hours after he insecticide-fungicide is sprayed can reach up to 98%. The product is an efficient, slightly toxic, growth-promoting and wide-effect biological insecticide-fungicide, can effectively relieve pest resistance, increase the nutrition and yield, and has no toxicity or residue. The product has specific effects on Lepidoptera pests such as borer, busck, cabbage caterpillar and fall webworm.

The invention discloses bacillus methylotrophicus wettable powder which comprises 100 parts by mass of bacillus methylotrophicus active components, 4-10 parts by mass of a wetting agent and 4-10 parts by mass of a dispersing agent. A method for preparing the bacillus methylotrophicus wettable powder comprises the following steps: weighing the bacillus methylotrophicus active ingredients, the wetting agent and the dispersing agent according to a ratio, and uniformly mixing; and milling through a jet mill, thereby obtaining the bacillus methylotrophicus wettable powder. The wettable powder is stable in quality and convenient to preserve and use for a long time, has good effects of preventing and treating cucumber gray mold and apple rot, solves the problems such as environmental toxic and side effects and pesticide residues brought by chemical agents, has the characteristics of high efficiency, no toxicity, safety and no residue, is a safe, effective, low-residue and environment-friendly microbial pesticide and has wide application prospects in the field of microbial control.

The invention provides a compound Chinese herbal medicinal bactericide, relating to the field of pesticides. The formula of the bactericide comprises 2-8 parts of pubescent holly root, 1-8 parts of amur corktree bark, 1-9 parts of scutellaria laterifolia, 3-11 parts of rheum officinale, 7-12.5 parts of indigowoad leaf, 6-11.5 parts of lithospermum, 9-13.5 parts of Wufei, 9.5-15 parts of honeysuckle, 6-11.5 parts of fructus forsythiae, 5-11 parts of dandelion, 5-10.5 parts of corydalis bungeana and 3-7 parts of coptis chinensis. The bactericide has the following beneficial effects: the bactericide overcomes the defects of single sterilization functions and slow effects of other biological pesticides, not only is wide and fast in sterilization and is free of residues but also can control various bacterial plant diseases, is not resistant to drugs after long-term use, is harmless to plants, people and livestock and is environment-friendly.

The invention relates to a pesticide compound for preventing plant disease, wherein said compound comprises emodin monomethyl ether and chrysophanol at 1:1-1:9 mass percentages; the compound can be obtained from Polygonaceae and soybean plant Chinese drug or made from manual purification or manual composition. Said compound added with other carrier and agent can be made into liquid, powder, emulsion, etc, whose effective component is 0.1-30%. The invention can generate sub metabolite with significant bacterial resistance.

The invention discloses a eugenol biological pesticide which comprises the following ingredients in percentage by weight: 0.1% to 70% of eugenol, 6% to 20% of surfactant, 0.01% to 0.5% of plant immunity regulator, 10% to 50% of synergist, and raw materials of alcohol and turpentine for the rest part. With the advantage of reasonable formula, the eugenol biological pesticide is characterized in that the active volatility of the active ingredient eugenol is improved, the pesticide effect of the eugenol is enhanced, pesticide doses are reduced, and the pesticide cost for farmers is reduced; pureplant solvents are used in the eugenol biological pesticide which belongs to low-carbon and environment-friendly pesticide, so pollution and harm to the environment are reduced, and the eugenol biological pesticide is safe and environment friendly for use. The eugenol biological pesticide is used for preventing and treating powdery mildew, botrytis and downy mildew and regulating plant immunity, and has the effects of synergy, simultaneous treatment, resistance prevention, resistance delay, etc.

A biological product in the form of liquid or wettable powder for preventing and eliminating the bacterial soft rot of konjak is prepared from the Bacillus amyloliquefaciens C3 (CGMCC No.1553) through liquid fermenting to obtain the cultured substance, adding wetting agent, adhesive and additive, and preparing liquid or wettable powder.

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

The invention relates to a natural green bio-organic pesticide composition and its application. The technology is characterized in that: A, natural camphor, rheum officinale, Chinese cassia tree, eucalyptus oil, dry ginger, common fennel fruit, capsicum and auxiliary materials are made into a natural biological pesticide; and B, rhizoma atractylodis, magnolia cortex (prepared with ginger), Poria cocos, raw Pinellia tuber, oil of patchouli,dried orange peel, dahurian angelica root, areca peel, extractum glycyrrhizae, perilla leaf oil and auxiliary materials are made into a natural biological pesticide. The pesticide provided by the invention can be directly applied to melon, fruit, leaf, vegetable legumes, tea, traditional Chinese medicines, flower, rape, cotton, tobacco and the like for the control of insect diseases. After applied with the biological pesticide, vegetables and fruits can be directly eaten without residual pesticide harm to human body.