511 results about "Lipopeptide" patented technology

The invention relates to a method for preparing a bacillus subtilis lipopeptid biosurfactant, which is characterized in that the adopted bacterial strain is efficient lipopeptid producing strain bacillus subtilis BIT09S1,BIT09S2 and BIT09A2, and the lipopeptid biosurfactant is prepared by taking cheap molasses, pulse flour, konjac gum finemeal, guar gum and gum breaking liquid of modified guar gum as carbon sources for the culture medium; adding other nitrogen sources, phosphorus sources, inorganic salt components and nutrient elements into the culture medium; and carrying out fermentation, separation and purification. The raw materials used for producing lipopeptid by the method are easily obtained and can be enormously purchased. The crude extract of the obtained lipopeptid biosurfactant Surfactin can be used in various drilling and production processes such as oil-gas field fracturing, acidizing, de-plugging, controlling and water shut-off, oily water treatment, environment renovation and the like, and the biosurfactant has wide prospect.

The invention relates to a bacillus amyloliquefaciens growing in a disease-preventing and growth-promoting plant and application thereof, relating to bacillus amyloliquefaciens and the application thereof. The bacillus amyloliquefaciens TF28 growing in the disease-preventing and growth-promoting plant belongs to bacillus, has the storage number of CGMCC No.4038 and the storage data of July 26, 2010. The application of the bacillus amyloliquefaciens TF28 comprises the following steps of: (1) preparing activated bacilli liquid; (2) inoculating the activated bacilli liquid in an NYD culture fluid to obtain a strain fermentation solution; and (3) soaking seeds in a dilution solution 50 times of the strain fermentation solution. The strain TF28 in the invention can be colonized, reproduced and transmitted in plants, can simultaneously generate two antibacterial substances, i.e. antimicrobial proteins and lipopeptide antibiotic and has the multiple efficacy of preventing diseases, stimulating growth, increasing the yield, improving the quality, widening an antibacterial spectrum, and the like. Rice seeds treated by the dilution solution 50 times of the fermentation solution of the strain TF28 reach a room-temperature bakana resistance rate over 84.6 percent.

The invention discloses a preparing method of new type lipopeptide biosurfactant surfactin and appliance, which comprises the following steps: utilizing bacillus subtilis strain E8 (conserved in Chinese microbe conservative council common microbe center, the keeping number at 1107) to proceed microbe liquid ferment prepare surfactant surfactin; proceeding carbon source, nitrogen source and inorganic salt optimize for the needed liquid culture medium; getting fermentation liquor; centrifuging to remove thallus; acidifying supernatant; extracting rough extract; further-extracting through dissolvent; acidifying; collecting deposition; centrifuging; vacuum-drying; getting pre-refined product; freeze-drying through drainage pillar and FPLC extraction; getting product with high purity; making the output of acidifying extraction at 24-56 h reach 5-13g / L. The dominant fermentate molecular weight of high-yield strain E8 can reach 1022Da and CMC can reach 1 mu M, which is the lowest of reported surfactin CMC value.

The present invention provides a novel class of detergents referred to herein as lipopeptide detergents. Lipopeptide detergents comprise an amphipathic α-helical peptide having a hydrophobic or neutral face and a hydrophilic face. To each end of this peptide is covalently linked an aliphatic hydrocarbon tail, these aliphatic tails being linked thereto such that they associate with the hydrophobic or neutral face of the peptide. Lipopeptide detergents can advantageously be used to stabilize membrane proteins in the absence of a phospholipid bilayer in a manner that preserves the native conformation and permits the subsequent crystallization thereof.

Provided are novel lipoglycopeptides exhibiting bacterial signal peptidase inhibitory activity. Also provided are actinomycete strains that produce these lipoglycopeptides, methods of producing such lipoglycopeptides by culturing these strains, pharmaceutical compositions comprising these lipoglycopeptides, and methods of treating bacterial infections comprising contacting bacteria susceptible to treatment with the present bacterial signal peptidase inhibitor lipoglycopeptides. These lipoglycopeptides are useful in human and veterinary medicine.

The invention concerns mixed micelles or micro-aggregates for inducing an immune response containing at least a first lipopeptide comprising a CTL epitope and at least a first lipid motif; and a second lipopeptide comprising at least an auxiliary T epitope and at least a lipid motif, whereof the type can be different from the first lipopeptide motif. Said micelles can be used as medicines and vaccines.

The invention discloses a lipopeptide biosurfactant, a preparation method thereof, and an application thereof. The method for preparing the lipopeptide biosurfactant comprises steps that: bacillus subtilis is fermented, such that a fermentation broth is obtained; and the lipopeptide biosurfactant is obtained. When the lipopeptide biosurfactant is formulated with other surfactants, a good synergic effect is provided. As a result of a core oil-displacement experiment employing an alkali-surfactant-polymer flooding complex system of the lipopeptide biosurfactant, water content can be greatly reduced, and recovery efficiency can be improved.

The invention provides methods for administering a therapeutically effective amount of daptomycin while minimizing skeletal muscle toxicity. The methods provide daptomycin administration at a dosing interval of 24 hours or greater. This long dosing interval minimizes skeletal muscle toxicity and allows for higher peak concentrations of daptomycin, which is related to daptomycin's efficacy. The invention also provides methods of administering lipopeptide antibiotics other than daptomycin while minimizing skeletal muscle toxicity by administering a therapeutically effective amount of the lipopeptide antibiotic at a dosage interval that does not result in muscle toxicity. The invention also provides methods of administering quinupristin / dalfopristin while minimizing skeletal muscle toxicity by administering a therapeutically effective amount of quinupristin / dalfopristin at a dosage interval that dos not result in muscle toxicity.

An oil-in-water emulsified composition suitable for external preparation for skin and cosmetic product, which comprised 0.1 to 5% by mass of (A) lipopeptide compound derived from a microorganism such as surfactin and its analogous compound, 0.05 to 1.5% by mass of (B) xanthan gum, 25 to 70% by mass of (C) oil component and (D) water, free from non-ionic surfactants and acrylic water-soluble polymers, and is excellent in feeling upon use, moisture retention, emollient property and stability as well as environmental suitability and safety for living organisms, and external preparation for skin and cosmetic product using the composition.

Bacteria of the sp. Bacillus that produce a lipopeptide are found to be effective in the treatment and prophylaxis of gastro-intestinal disease when administered as a probiotic. In particular, a strain of Bacillus bacteria identified as PB6 is useful for the treatment of Antibiotic Associated Diarrhea (AAD) or the more serious condition Clostridium difficile associated diarrhea (CDAD) when administered as a probiotic. Additionally, these bacteria have been found efficient for the treatment of immunorelated diseases such as Inflammatory Bowel Disease (IBD).

The present invention relates to crystalline and crystal-like forms of lipopeptides, including daptomycin, a lipopeptide antibiotic with potent bactericidal activity against gram-positive bacteria, including strains that are resistant to conventional antibiotics. The present invention relates to methods of purifying lipopeptides, including daptomycin, a lipopeptide antibiotic with potent bactericidal activity against gram-positive bacteria, including strains that are resistant to conventional antibiotics. The present invention also relates to pharmaceutical compositions comprising the purified form of the lipopeptide and methods of using these compositions.

The invention relates to a production method of bacillus subtilis antimicrobial lipopeptide and application thereof in feeds, belonging to the fields of feed additives and the biotechnology. The production method of the bacillus subtilis antimicrobial lipopeptide comprises the following steps of carrying out amplification culturing on an acillus subtilis fmbj seed culture medium, inoculating by 2-5 percent at the temperature of 28-35 DEG C, deeply fermenting 5000L of fermentor liquid for 30-48h, then extracting, and carrying out spray drying after concentration or directly carrying out spray drying to produce an antibacterial peptide product. The antimicrobial lipopeptide is added into basic daily feeds for broilers by a certain titer, thereby enhancing the feed utilization ratio and having better effect on growing development and immunity strengthening of broilers. The bacillus subtilis is a safe microorganism, and an antibacterial product thereof has high safety. In addition, compared with flavomycin used in the traditional broiler feeds, the bacillus subtilis antimicrobial lipopeptide has better effect and has an important significance on replacing feed antibiotic addition and guaranteeing food safety.

The present invention relates to novel lipopeptide compounds. The invention also relates to pharmaceutical compositions of these compounds and methods of using these compounds as antibacterial compounds. The invention also relates to methods of producing these novel lipopeptide compounds and intermediates used in producing these compounds.

The present invention relates to biological bacillus lipopeptide pesticide and its application, and belongs to the field of biotechnology. The lipopeptide pesticide is prepared through fermentation culture in culture medium of glucose, cane sugar or corn slurry at temperature of 30-37 deg.c, rotation speed of 300-1000 rpm and dissolved oxygen of 30% for 30-36 hr; centrifugation to collect supernatant, adding HCl to regulate pH to 2.0-2.5, centrifugal extraction to collect supernatant, methanol or ethanol extraction, merging extracted liquid, and vacuum drying. The present invention is used in the green prevention and control of pests and diseases of field.

Lipophilic conjugates comprise a peptide coupled to a fatty acid The peptide comprises at least two positively charged amino acid residues; the peptide after conjugation to the fatty acid possessing antibacterial, antifungal, and / or anticancer activity higher than prior to conjugation. The lipophilic conjugates are suitable for treatment of infections caused by pathogenic organisms such as bacteria and fungi. The lipophilic conjugates are also suitable for sanitation, as disinfectants, or for food preservation.

The present invention provides a method for allowing foreign particles to penetrate, very efficiently, the cell wall, cell membrane, organelle membrane and / or nuclear membrane of a cell and hybridizing or binding to the complimentary target in the cell. The cells may be from a culture or from specimens obtained from a patient. The foreign particle can be a probe consisting of, for example, either individually or in any combination of two or more of the following: DNA, RNA, peptide nucleic acids (PNA), glycopeptides, lipopeptides, glycolipids or prions. The target is a cell, a cell component or, preferably, a pathogen or pathogen component. The pathogen can be, for example, bacteria, fungi, yeast or viruses.

The present invention provides synthetic immunogenic lipopeptide molecules comprising co-linear T-helper and B cell epitopes, and methods for their production and use in the generation of primary and secondary immune responses, and for the vaccination of animal subjects against particular antigens. More particularly, the present invention provides highly soluble lipopeptides wherein the lipid moiety is attached to the terminal side-chain group of an internal lysine or lysine analog, preferably to the terminal side-chain group of an internal diamino acid residue. Preferably the internal lysine or lysine analog is positioned between the T-helper epitope and the B cell epitope or within the T-helper epitope.

The invention relates to a method for the enzymatic elimination of the N-acyl side chain from lipopeptides to form the corresponding nucleus, wherein the lipopeptide is prepared by fermentation, the lipopeptide being bound to the cells of the biomass, and the biomass is removed with the adhering lipopeptide, the biomass with the adhering lipopeptide is resuspended in an aqueous system, a suitable deacylase is added in dissolved or solid form to the suspension of the biomass, and the corresponding nucleus is formed, and the nucleus is optionally isolated and purified, wherein the lipopeptide obtained by fermentation is reacted after the end of the fermentation as cell-bound biomass without further purification directly with a deacylase, whereby the N-acyl chain linked via an amide linkage is eliminated.

The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.

The invention provides bacillus amyloliquefaciens HAB-2, which has the collection number of CCTCC M 2015070. Although the key lipopeptide synthetase gene sfp gene is deleted, the surfactin substances can still be generated, indicating that the strain has a specific and distinctive biosynthetic pathway and has a remarkably high research value. The bacillus amyloliquefaciens HAB-2 has very strong ability of inhibiting pathogenic fungi, has a wide antibacterial spectrum and can inhibit multiple kinds of pathogenic fungi while the antibacterial effect is good and the antibacterial rate is high. Further research finds that the active ingredient can be directly obtained through simple treatment on the fermentation broth of the strain, and the antibacterial active ingredient is stable and has very high activity in strong acid and strong alkaline environments; the antibacterial activity is not changed after 24 hours of ultraviolet irradiation, and high temperature does not influence the antibacterial active ingredient; and the fermentation broth of the biocontrol bacteria realizes certain growth promoting effect on a plant.

The invention discloses a bacillus and application thereof, and belongs to the field of biological control. A bacillus strain is bacillus methylotrophicus C1, and a preservation number of the bacillus strain is GDMCC No.60046. The application includes that the bacillus can be used as a biological control strain to be applied to antagonistic crop pathogenic bacteria; the bacillus can be used as a lipopeptide and / or protein fermentation strain for inhibiting crop pathogenic bacteria; the bacillus can be used as a growth promotion strain to be applied to promoting growth of crops; the bacillus can be used as a fermentation strain for auxin. The bacillus and the application have the advantages that the strain has broad-spectrum antibacterial activity; lipopeptide substances with effects of inhibiting fusarium graminearum cannot be generated, but proteins with effects of inhibiting the fusarium graminearum can be generated; effects of promoting growth of corn can be realized by the strain C1, the root lengths and the stalk lengths of the corn of experiment groups are 3.15 and 3.05 times the root lengths and the stalk lengths of contrast groups respectively as compared with contrast, the root, stalk and seed weights of the corn of the experiment groups are 5.02, 4.33 and 1.4 times the root, stalk and seed weights of the contrast groups, and only little auxin can be generated by the strain C1.

New synthetic Lipid-A analogs based on monosaccharide (1) and disaccharide (2) derivatives were designed and prepared in the present invention. Both structures (1) and (2) incorporate novel lipid structures (3) and (4) that are not found in nature. Also, novel disaccharide Lipid-A structures (2) that incorporate novel contingents of uniform lipids and where R1, R4 and R5 are the same substitution group of structure (III) were synthesized. Liposome formulations containing totally synthetic components such as synthetic Lipid-A and synthetic lipopeptide derived from tumor-associated MUC1 mucin are described along with their therapeutic utility. Comparative test results of immunostimulating properties and toxicity of Lipid-A analogs (1) and (2) are included.

There is provided a lipid peptide that is capable of forming a hydrogel with an extremely small amount thereof over a liquid property range from acidic to alkaline, and a hydrogel having high environmental suitability, biocompatibility and biodegradability. A lipid peptide represented by Formula (1):(where R1 represents an aliphatic group having 9 to 23 carbon atoms; R2, R3, R4 and R5 independently represent a hydrogen atom, an alkyl group having 1 to 7 carbon atom(s) which optionally has a branched chain having 1 to 3 carbon atom(s), a phenylmethyl group, a phenylethyl group or a —(CH2)—X group, and at least one of R2, R3, R4 and R5 represents a —(CH2)n—X group;n represents the number of 1 to 4; X represents an amino group, a guanidine group, a —CONH2 group or a 5-membered ring, a 6-membered ring or a fused heterocyclic ring composed of a 5-membered ring and a 6-membered ring which optionally have 1 to 3 nitrogen atom(s); and in represents 1 or 2), and a hydrogel comprising the lipid peptide.

The related preparation method for antibiotic peptide comprises: with ES-2 strain of Bacillus amyloliquefaciens, amplifying culture with medium, taking 2% inoculation, and deep fermenting for 30-48h at 28-35h, or taking inoculation with solid fermentation medium for culturing 2-4day at 28-37Deg; extracting, concentrating, adsorbing, and drying to obtain the product with molecular weight as 994, 1008, 1022, 1036 and 1449, 1463, 1477, 1491 Da as the mixture of surfactin and fangycin. This product can bear heating at 100Deg for 30min, keeps activity in pH 2-12, and can be used as food preservative and additive.

The invention relates to an anhydrous foaming aerosol for removing makeup. The anhydrous foaming aerosol is characterized by comprising the following components in percent by weight: 2.5-7% of polyglycerol-2 laurate, 1.5-6% of polyglycerol-5 trioleqate, 0.1-1% of sodium bacillus-subtilis lipopeptide, 60-80.8% of soothing oil, 0.1-1% of antioxidant and 15-25% of propellant. The anhydrous foaming aerosol has the advantages that foam is soft and dense without easy flowing and dripping, the use is convenient, the makeup removing capability is strong and the skin is moisturized without dryness after makeup removal.

The present invention provides a method for allowing foreign particles to penetrate, very efficiently, the cell wall, cell membrane, organelle membrane and / or nuclear membrane of a cell and hybridizing or binding to the complimentary target in the cell. The cells may be from a culture or from specimens obtained from a patient. The foreign particle can be a probe consisting of, for example, either individually or in any combination of two or more of the following: DNA, RNA, peptide nucleic acids (PNA), glycopeptides, lipopeptides, glycolipids or prions. The target is a cell, a cell component or, preferably, a pathogen or pathogen component. The pathogen can be, for example, bacteria, fungi, yeast or viruses.

The invention relates to separation and purification of antifungal lipopeptide of marine microorganisms, in particular to a method for separating and purifying the lipopeptide of anti-plant-fungal diseases. The method comprises the following steps that: (1) a marine bacillus subtilis3512A is adopted to conduct fermentation and culture under the suitable condition; (2) centrifugation, acid precipitation and methyl alcohol extraction are conducted after fermentation and culture, and then lipopeptide crude extract is obtained; (3) silica gel high-speed chromatography is adopted to collect active components with antifungal property; and (4) RP-HPLC is utilized to prepare and obtain an antifungal lipopeptide pure product. The invention effectively provides a method which is separated and purified from the metabolite of the marine bacillus subtilis and has anti-plant-pathogenic-fungi lipopeptide, and has the advantages of fast, simple and convenient operation, good purification effect, difficult loss of activity, low cost and the like.

The invention relates to an extracellular antibacterial lipopeptide of plant endophytic Bacillus subtilis Jaasedl and a separation and purification method. The distribution range of the molecular weight of the extracellular antibacterial lipopeptide is mainly between 1,000Da and 2,200Da. The extracellular antibacterial lipopeptide contains an Iturin homologue, a Fengycin homologue and a Surfactin-like Compound homologue. The extracellular antibacterial lipopeptide is a group of uncommon antibacterial lipopeptide mixture produced by a single bacterial strain. The separation and purification method of the extracellular antibacterial lipopeptide comprises the following steps of: after salting out to obtain the crude extract of the antibacterial lipopeptide by using ammonium sulfate from the fermentation liquor of the endophytic Bacillus subtilis Jaasedl, performing Sephedex G-25 molecular sieve chromatography, Cellulose DEAE-52 anion exchange chromatography and FPLC300SB-C18 column chromatography successively, wherein 34 to 37 min of collecting peak has bacteriostatic activity; detecting by Tricine-SDS-PAGE after concentrating; and achieving electrophoretically pure at only one strip to obtain pure extracellular antibacterial lipopeptide. The antibacterial lipopeptide has extremely high research and application value for the development of broad-spectrum antifungal medicaments, and has wide application prospect for natural quality protection of crops and pesticide residue reduction.

The invention relates to a method for quantifying indigenous microorganisms for producing lipopeptide surfactant in microbial flooding reservoir. Primers are designed according to lipopeptide synthetase genes of microorganisms for producing lipopeptide surfactant, and fluorescent quantitative PCR (polymerase chain reaction) is performed. In optimized reaction procedures and reaction system, the lipopeptide synthetase genes obtained from a standard lipopeptide-producing strain is connected with a carrier to construct a recombinant plasmid used as a standard sample for real-time quantitative PCR; the standard sample is subjected to 10-time gradient dilution and used as a template for fluorescent quantitative PCR, and a standard curve is drawn; a microbial flooding reservoir sample is subjected to simple treatment and used as a template for fluorescent quantitative PCR; and whether data of replicate samples are identical and whether initial templates having different numbers of copies have the same amplification efficiency are judged according to linear relationship R2 and amplification efficiency E. When being used for detecting microorganisms for producing lipopeptide surfactant in reservoir under optimized reaction conditions, the lipopeptide synthetase specific primers have higher stability and sensitivity, thereby being widely used in the field of microbial enhanced oil recovery.

The invention relates to a microbe generating antifungal lipopeptid and a preparation method and application of the antifungal lipopeptid thereof, wherein a strain generating the antifungal lipopeptid through microbial fermentation is classified to belong to Bacillus subtilis BI1, and the collection number is CGMCC No. 3412. The invention also relates to a preparation method of fermented antifungal lipopeptid, comprising the following steps of: inoculating cultured seeds into a fermentation culture medium according to 1%-10% (v / v) of inoculation quantity by adopting fermentation strain BI1, wherein culture temperature is 30-40 DEG C, fermentation ventilatory capacity is 5-13 m<3> / h, tank pressure is 0.02-0.09 MPa, stirring speed is 100-150 turns / minute, and fermentation time is 30-60 hours; introducing sterile air in the fermentation process; and maintaining the pH of fermentation liquor between 6.5 and 7.5 by using ammonia water or liquid caustic soda, wherein the output of the antifungal lipopeptid in the fermentation liquor is 1-2 g / L. The invention also discloses the application of the antifungal lipopeptid in preparing drugs treating dermatophytosis.